Biomaterial-based nanocomposites have been increased in demand in industries for a purpose of packaging and sensing because of their low cost and environmentally friendly nature. But less work has been done on conductive, biodegradable film preparations. This article is devoted to the preparation of Polyaniline/ Carboxymethyl guar gum nanocomposites film and their applications. Controlled synthesis of polyaniline in the presence of carboxymethyl guar gum solution has been carried out for the synthesis of these nanocomposites. Then nanocomposite films have been obtained by solution casting method in one step. The object of this research work was to prepare a conductive, biodegradable, flexible film. Such made nanocomposites film has been further tested for FTIR, SEM, UTM, Conductivity, Swelling behavior and biodegradability. The results have been concluded that by this method a fine flexible, conductive, biodegradable film may be prepared that can be applied in different fields for further study.