Bovine Serum Albumin Solution Research Articles

Influence of PEG-PPG-PEG Block Copolymer Concentration and Coagulation Bath Temperature on the Structure Formation of Polyphenylsulfone Membranes.

The effect of amphiphilic block copolymer polyethylene glycol (PEG)-polypropylene glycol (PPG)-PEG concentration in the polyphenylsulfone (PPSU) casting solution and coagulation bath temperature (CBT) on the structure, separation, and antifouling performance of PPSU ultrafiltration membranes was studied for the first time. According to the phase diagram obtained, PPSU/PEG-PPG-PEG/N-methyl-2-pyrrolidone (NMP) systems are characterized by a narrow miscibility gap. It was found that 20 wt.% PPSU solutions in NMP with the addition of 5-15 wt.% of PEG-PPG-PEG block copolymer feature upper critical solution temperature, gel point, and lower critical solution temperature. Membrane composition and structure were studied by Fourier-transform infrared spectroscopy, scanning electron and atomic force microscopies, and water contact angle measurements. The addition of PEG-PPG-PPG to the PPSU casting solution was found to increase the hydrophilicity of the membrane surface (water contact angle decreased from 78° for the reference PPSU membrane down to 50° for 20 wt.%PPSU/15 wt.% PEG-PPG-PEG membrane). It was revealed that the pure water flux increased with the rise of CBT from 18-20 L·m-2·h-1 for the reference PPSU membrane up to 38-140 L·m-2·h-1 for 20 wt.% PPSU/10-15 wt.% PEG-PPG-PEG membranes. However, the opposite trend was observed for 20 wt.% PPSU/5-7 wt.% PEG-PPG-PEG membranes: pure water flux decreased with an increase in CBT. This is due to the differences in the mechanism of phase separation (non-solvent-induced phase separation (NIPS) or a combination of NIPS and temperature-induced phase separation (TIPS)). It was shown that 20 wt.% PPSU/10 wt.% PEG-PPG-PEG membranes were characterized by significantly higher antifouling performance (FRR-81-89%, DRr-26-32%, DRir-10-20%, DT-33-45%) during the ultrafiltration of bovine serum albumin solutions compared to the reference PPSU membrane prepared at different CBTs (FRR-29-38%, DRr-6-14%, DRir-74-89%, DT-88-94%).

Protein Interaction and Molecular Docking Analysis by Schiff Base Derived from 2,4-Dinitro Phenyl Hydrazine

A novel and cost-effective 2,4-dinitrophenyl hydrazine derived from Schiff base 1-benzylidine-2-(2,4-dinitrophenyl) hydrazine ( L) was designed and characterized using various spectroscopic techniques. The interaction between L and bovine serum albumin (BSA) has been carried out using UV–Vis and fluorescence spectroscopy, DSC, SEM, and molecular docking methods. The phosphate buffer ([Formula: see text]) solution of BSA showed fluorescence emission maxima at 342[Formula: see text]nm. Upon addition of L to the BSA solution, it quenched the fluorescence emission at 342[Formula: see text]nm. The quenching of the fluorescence emission is due to the formation of a complex between L and BSA. The binding constant was calculated from the fluorescence titrations and found to be [Formula: see text][Formula: see text]M[Formula: see text]. Further, molecular docking analysis was carried out to establish the binding between L and BSA.

Scalable, flexible, ultra-strong, free-standing papery all carbon nanotube membrane with excellent separation and antifouling properties

Carbon nanotube (CNT) based membranes have shown tremendous promise for water purification. However, fabricating free-standing all CNT membranes with excellent separation and antifouling properties remains challenging. Here, a densified flexible, ultra-strong, free-standing papery all CNT membrane with excellent separation and antifouling properties is reported. The chemically densified free-standing CNT membrane has an extremely high tensile strength up to 330 MPa, four times higher than the original CNT membrane and one order of magnitude higher than most polymeric membranes. The negatively charged surface of the densified CNT membrane enables efficient separation of two dyes even with similar molecular sizes. Meanwhile, the densified CNT membrane exhibits superior antifouling performance during the filtration of dyes, humic acid and bovine serum albumin solutions, and oil-in-water emulsions due to its reinforced hydration layer induced by the hydrophilic functional groups resulting from chemical densification. The chemically densified CNT membrane shows not only excellent separation and antifouling performance, but also superior long-term stability and regeneration performance in the filtration of foulant-containing solutions. This study may pave the way for large-scale application of densified free-standing all CNT membranes in the realm of water treatment.

Unravelling protein corona formation on pristine and leached microplastics

Upon entering the environment, the surface properties of pristine plastics are rapidly altered due to interactions with exogenous biomolecules, contaminants, and even microbiota, which ultimately alter their ecological impacts. When present in biological fluids or high protein environments, micro(nano)plastics bind with proteins, which form a protein corona around the particle. Although a significant body of literature exists on protein corona formation on nanomaterials, less is known about how the physiochemical properties of microplastics may influence protein corona formation. This study utilises quantitative proteomics to quantify protein binding to pristine and leached microplastics. Pristine polyethylene (PE) beads (50 and 500 μm), polyamide (PA) fibres (100 μm), polyethylene terephthalate fibres (500 μm), and fragments (< 300 μm), as well as pristine and leached textile microfibres comprised of PET, recycled PET, PA or cotton were incubated for 24 h in bovine serum albumin solution (2 mg mL−1) to form a protein corona. Protein adsorption to microplastics was dependant on particle surface area to volume ratio but only when additives were absent. For environmentally relevant textile microfibres, cotton microfibres adsorbed significantly more protein than synthetic microfibres. Fourteen-day aqueous leaching increased the zeta potential of all microfibres. However, only PA fibres adsorbed significantly higher protein on the leached fibres compared to their pristine counterparts. Overall, the presence of chemical additives in microplastics strongly influenced protein corona formation, and this phenomenon should be incorporated into routine microplastic toxicity assessment.Graphical

Synthesis and characterization of a nanostructure conductive copolymer based on polyaniline and polylactic acid as an effective substrate in proteins impedimetric biosensing.

Despite of all the developments in DNA microarray technology, there is not sufficient knowledge about protein abundance or their function in processes such as proteolysis, phosphorylation. Therefore, there is a significant need for direct detection and quantification of proteins, especially in processes such as proteomics, drug design and disease prediction. The present work introduce the new generation of polymeric substrate based on polyaniline and, polylactic acid, which it was used for impedimetric sensor in detection of proteins in particular for bovine serum albumin (BSA). In this copolymerization, the polylactic acid-block-polyaniline copolymer (PLA-b-PANI) was synthesized to attach polylactic acid and polyaniline using epichlorohydrin as a coupling agent. The structure of synthesized compounds in all steps, were confirmed by FT-IR and, 1H-NMR. The thermal properties and, morphology were analyzed by DSC, TGA, and, SEM. Also the electrochemical characteristics of fabricated PLA-b-PANI electrode were investigated by Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV). The results demonstrated that morphology of the PLA-b-PANI is sphere shape nanoparticles with dimension less than 100 nanometer diameters and, reasonable thermal properties. PLA-b-PANI was used to modify a screen-printed carbon electrode (SPCE) to fabricate a BSA impedimetric sensor. In order to increase the performance of the proposed impedimetric sensor, optimization of incubation time, pH and amount of PLA-b-PANI were investigated. The results show that the impedimetric sensor has the highest response when the electrode surface is covered with 5 microliters of PLA-b-PANI, and is incubated in BSA solution with pH 6.5 for 5 min. Impedimetric results showed that the PLA-b-PANI has excellent properties in reducing the charge transfer resistance and increasing the electron charge transfer rate. The final impedimetric sensor exhibited good repeatability, reproducibility, and chemical stability within the linear concentration range of 0.1-20 μg L-1 of BSA, and a detection limit of 0.05 μg L-1.

Design, Fabrication and Performance Analysis of a Portable, Antenna Analyzer Based, Quartz Crystal Microbalance Measuring System with Energy Dissipation

Impedance measurements play a critical role in analyzing the electrical behavior of piezoelectric biosensors in general. Antenna analyzers are engineered to measure the specific case of input impedance for antenna systems. In this study small form factor antenna analyzer is repurposed to work as driving circuit for a Quartz Crystal Microbalance (QCM) biosensor in combination with a single board computer as an indication of how small and portable an impedance measuring system can be made, while allowing monitoring of important parameters of series and parallel resonance frequencies together with dissipation factor. A QCM crystal with a 10 MHz fundamental resonance frequency is employed to determine the limit of detection of the system in Bovine Serum Albumin (BSA) and glycerol solutions. Dissipation factor and phase angle were monitored during the experiments. Limit of detection is 20 µg/ml BSA in phosphate buffer saline (PBS) and 250 µl of glycerol in 100 ml of deionized water.

Magnetic toroidal dipole resonance terahertz wave biosensor based on all-silicon metasurface

Optical sensing is crucial in many applications and has received widespread attention in recent years. Transmission spectroscopy with narrow linewidth resonances is indispensable for improving metasurface sensing performance. However, the narrower linewidth results in a smaller resonant modulation range, which seriously hinders the application of metasurfaces in sensing detection. We designed an all-silicon metasurface sensor. The structural layer of the metasurface works together with the substrate layer. Multiple magnetic ring dipoles are generated in the structural layer and multiple magnetic dipoles are generated in the substrate layer. The two work together to stimulate sharp Fano resonance, with a quality factor of up to 272.84. Based on the characteristics of metasurface, when applied to sensing and detection, the theoretical sensitivity can reach 59.64 GHz/RIU. Metasurface samples were successfully prepared using photolithography and dry etching. After characterizing the transmission spectrum of the prepared metasurface, the actual quality factor reached 76.8. At the same time, the metasurface sensor was used to detect bovine serum albumin (BSA) solutions of different concentrations. Based on the test results, it is calculated that the detection limit of the metasurface sensor for detecting BSA can reach 0.027 mg/mL, and the detection sensitivity can reach 9.7 GHz/(ng/mm2). The results of theoretical simulation and biological experiments show that the sensor has good sensing performance. This work is of great significance to the research of all-silicon metasurfaces and the application of metasurface sensors.

Concentration Dependence of Elastic and Viscoelastic Properties of Aqueous Solutions of Ficoll and Bovine Serum Albumin by Brillouin Light Scattering Spectroscopy.

The cellular environment is crowded with macromolecules of different shapes and sizes. The effect of this macromolecular crowding has been studied in a variety of synthetic crowding environments: two popular examples are the compact colloid-like Ficoll macromolecule and the globular protein bovine serum albumin (BSA). Recent studies have indicated that a significant component of bound or surface-associated water in these crowders reduces the available free volume. In this work, Brillouin light scattering experiments were performed on aqueous solutions of Ficoll 70 and Ficoll 400 with concentrations ranging from 1 to 35 wt % and BSA with concentrations of 1 to 27 wt %. From the dependence of spectral peak parameters on polymer concentration, we determined fundamental solution properties: hypersound velocity, adiabatic bulk modulus and compressibility, apparent viscosity, and hypersound attenuation. The existing theory that ignores intermolecular interactions can capture only the observed linear trends in the frequency shift up to a threshold concentration, beyond which a quadratic term accounting for intermolecular interactions is necessary. This likely indicates a transition from the dilute to semidilute regime. In the Ficoll solutions (but not BSA), we see evidence for a central mode, which is indicative of relaxation in the hydration shell of Ficoll.

Versatile Capillary Cells for Handling Concentrated Samples in Analytical Ultracentrifugation.

In concentrated macromolecular dispersions, far-from-ideal intermolecular interactions determine the dispersion behaviors including phase transition, crystallization, and liquid-liquid phase separation. Here, we present a novel versatile capillary-cell design for analytical ultracentrifugation-sedimentation equilibrium (AUC-SE), ideal for studying samples at high concentrations. Current setups for such studies are difficult and unreliable to handle, leading to a low experimental success rate. The design presented here is easy to use, robust, and reusable for samples in both aqueous and organic solvents while requiring no special tools or chemical modification of AUC cells. The key and unique feature is the fabrication of liquid reservoirs directly on the bottom window of AUC cells, which can be easily realized by laser ablation or mechanical drilling. The channel length and optical path length are therefore tunable. The success rate for assembling this new cell is close to 100%. We demonstrate the practicality of this cell by studying: (1) the equation of state and second virial coefficients of concentrated gold nanoparticle dispersions in water and bovine serum albumin (BSA) as well as lysozyme solution in aqueous buffers, (2) the gelation phase transition of DNA and BSA solutions, and (3) liquid-liquid phase separation of concentrated BSA/polyethylene glycol (PEG) droplets.

The dynamics of PEG‐coated nanoparticles in concentrated protein solutions up to the molecular crowding range

AbstractPolymer‐coated nanoparticles are widely studied in the context of nanomedicine and it is therefore of utmost importance to understand not only how their structure but also how their colloidal dynamics are affected by physiologically relevant conditions. A characteristic feature of the cytosol of cells is the very high concentration of proteins among other matrix components, often termed macromolecular crowding. Here, the structure and colloidal dynamics of poly(ethylene glycol) (PEG)‐coated gold nanoparticles in the presence of bovine serum albumin (BSA) concentrations ranging from 0 to 265 mg/mL are studied with X‐ray photon correlation spectroscopy. For protein–nanoparticle mixtures with high BSA concentrations, comparable to intracellular levels, a significant deviation of the apparent viscosity from expectations for pure BSA solutions is found. The findings strongly indicate that the nanoscopic viscous properties of the dense protein solutions are significantly affected by the nanoparticles. At these high concentrations, the colloidal stability of the samples depends on the molecular weight of the coating PEG–ligand, whereas at lower concentrations no differences are observed.

Development of a radiochemical test procedure for the separation of aerosol-borne allergens

An innovative radiochemical method has been developed to determine the filtration efficiency of HEPA filters for biological nanoparticles. The setup based on EN 1822, for flat sheet media, is up to 40 times more sensitive for a single particle than methods based on particle counting, even for low particle concentrations. To achieve such a high sensitivity the radioactive tracer technique is utilised using a solution of bovine serum albumin (BSA) labelled with radioactive iodine (131I). With the aid of an aerosol generator, this solution is nebulised into nanoparticles of a defined size, which are then passed to the test filter via a compressed air flow. On the raw and clean gas side of the test filter, the 131I is measured with two NaI(Tl) detectors. By balancing the measurement data with each other, the filter efficiency can be determined. Initially, the setup has been validated with current particle counting methods, according to EN 1822, to ensure its functionality. Subsequently, the first experiments with radiolabelled nanoparticles gave promising results, as will be shown below. Further experiments also yielded information about filter clogging and its influence on the filter efficiency.

A novel radiotracer method for filter testing

Pollen allergens pose a significant health burden, affecting up to 30 % of the population in industrialized countries (Pablos, I.; Wildner, S.; Asam, C.; Wallner, M.; Gadermaier, G. Pollen Allergens for Molecular Diagnosis. Curr. Allergy Asthma Rep. 2016, 16, 1–12). With sizes typically ranging from 15 to 150 μ mm (Wafaa, K.T. Pollen Allergens of some Road Trees, Shrubs and Herbs in Alexandria, Egypt. OAJBS.ID.000143, 2020), they can be filtered out of the air. However, recent studies have revealed that many pollen allergens are transported in free vesicles called pollensomes (Prado, N.; de Linares, C.; Sanz, M.L.; Gamboa, P.; Villalba, M.; Rodríguez, R.; Batanero, E. Pollensomes as Natural Vehicles for Pollen Allergens. J. Immun. (Balt., Md. : 1950) 2015, 195, 445–449, Rutter, B.D.; Innes, R.W. Extracellular Vesicles as Key Mediators of Plant-Microbe Interactions. Curr. Opin. Plant Biol. 2018, 44, 16–22). These vesicles, which have a much smaller size of approximately 28–60 nm (Prado, N.; de Linares, C.; Sanz, M.L.; Gamboa, P.; Villalba, M.; Rodríguez, R.; Batanero, E. Pollensomes as Natural Vehicles for Pollen Allergens. J. Immun. (Balt., Md. : 1950) 2015, 195, 445–449), cannot be retained by conventional filters. However, novel filter materials have been developed (Gotschall, K. Personal Communication; KLAVEGO GmbH & Co KG, 2022) which are capable of binding even such small protein structures selectively. Standard test methods exist for determining the retention efficiency for common filter technologies. However, these methods cannot be directly applied to the aforementioned nanoparticles. This limitation arises from the fact that the particle concentration of the filtered gas is often too low for conventional aerosol measurement devices to yield meaningful results. In this study, a novel test procedure using a solution of bovine serum albumin (BSA) labeled with radioactive iodine (I-131) is developed to determine the retention efficiency of filters by a highly sensitive radiotracer technique.

The effect of biologically active substances on BSA and on the textures of films obtained by drying water-salt solutions of BSA

In the previously published results, we studied the effect of flavin mononucleotide (FMN), sodium halides NaF and NaBr, iron chloride FeCl3 and aluminum chloride AlCl3 on zigzag patterns of films obtained from saline solutions of bovine serum albumin (BSA). Changes in the properties of the biopolymer and its aqueous environment in solutions were monitored by UV absorption and fluorescence spectroscopy, microwave dielectrometry and dynamic light scattering. This work summarizes the obtained results on the influence of components of various nature on BSA and its film textures. Changes in the parameters of zigzag patterns are associated with polydispersity due to the presence of colloidal particles, aggregates and FMN associates, as well as a decrease in the surface potential and BSA hydration. The possibility of predicting the character of the influence of the studied biologically active substances on BSA is analyzed.

Sustainable additive manufacturing of polysulfone membranes for liquid separations

Membranes serve as important components for modern manufacturing and purification processes but are conventionally associated with excessive solvent usage. Here, for the first time, a procedure for fabricating large area polysulfone membranes is demonstrated via the combination of direct ink writing (DIW) with non-solvent induced phase inversion (NIPS). The superior control and precision of this process allows for complete utilization of the polymer dope solution during membrane fabrication, thus enabling a significant reduction in material usage. Compared to doctor blade fabrication, a 63% reduction in dope solution volume was achieved using the DIW technique for fabricating similarly sized membranes. Cross flow filtration analysis revealed that, independent of the manufacturing method (DIW vs. doctor blade), the membranes exhibited near identical separation properties. The separation properties were assessed in terms of bovine serum albumin (BSA) rejection and permeances (pressure normalized flux) of pure water and BSA solution. This new manufacturing strategy allows for the reduction of material and solvent usage while providing a large toolkit of tunable parameters which can aid in advancing membrane technology.

What determines sub-diffusive behavior in crowded protein solutions?

The aqueous environment inside cells is densely packed. A typical cell has a macromolecular concentration in the range 90–450 g/L, with 5%–40% of its volume being occupied by macromolecules, resulting in what is known as macromolecular crowding. The space available for the free diffusion of metabolites and other macromolecules is thus greatly reduced, leading to so-called excluded volume effects. The slow diffusion of macromolecules under crowded conditions has been explained using transient complex formation. However, sub-diffusion noted in earlier works is not well characterized, particularly the role played by transient complex formation and excluded volume effects. We have used Brownian dynamics simulations to characterize the diffusion of chymotrypsin inhibitor 2 in protein solutions of bovine serum albumin and lysozyme at concentrations ranging from 50 to 300 g/L. The predicted changes in diffusion coefficient as a function of crowder concentration are consistent with NMR experiments. The sub-diffusive behavior observed in the sub-microsecond timescale can be explained in terms of a so-called cage effect, arising from rattling motion in a local molecular cage as a consequence of excluded volume effects. By selectively manipulating the nature of interactions between protein molecules, we determined that excluded volume effects induce sub-diffusive dynamics at sub-microsecond timescales. These findings may help to explain the diffusion-mediated effects of protein crowding on cellular processes.

Biosensor based on multiple metal layer-hole array

We propose a biosensor which is sensitive to the refraction index (RI) of the tested liquid. The sensor is composed of a metal-insulator-metal structure based on periodic nanopore arrays, whose absorption spectrum exhibits a narrow peak at the resonant wavelength. The finite difference time domain method is used to simulate the proposed structure. The results show that there is a narrow resonant peak at the wavelength of 800 nm with RI of 1.33. By adding grooves on the lower metal layer, the maximum absorption rate increases to 99.7%. When RI varies in the range from 1.33 to 1.35, it can have a high sensitivity of 550 nm /RIU, a narrow half peak full width of 5.87 nm, and a figure of merit of 93.7. In addition, the absorption peak value also changes significantly with a sensitivity of 1845%/RIU, which can be used to measure the concentration of bovine serum albumin and other solutions to achieve biosensors.

Induced Circular Dichroism Analysis of Thermally Induced Conformational Changes on Protein Binding Sites Under a Crowding Environment.

Protein-ligand interactions in crowded cellular environments play a crucial role in biological functions. The crowded environment can perturb the overall protein structure and local conformation, thereby influencing the binding pathway of protein-ligand reactions within the cellular milieu. Therefore, a detailed understanding of the local conformation is crucial for elucidating the intricacies of protein-ligand interactions in crowded cellular environments. In this study, we investigated the feasibility of induced circular dichroism (ICD) using 8-anilinonaphthalene-1-sulfonic acid (ANS) for local conformational analysis at the binding site in a crowding environment. Bovine serum albumin (BSA) concentration-dependent measurements were performed to assess the feasibility of ANS-ICD for analyzing protein interior binding sites. The results showed distinct changes in the ANS-ICD spectra of BSA solutions, indicating their potential for analyzing the internal conformation of proteins. Moreover, temperature-dependent measurements were performed in dilute and crowding environments, revealing distinct denaturation pathways of BSA binding sites. Principal component analysis of ANS-ICD spectral changes revealed lower temperature pre-denaturation in the crowded solution than that in the diluted solution, suggesting destabilization of binding sites owing to self-crowding repulsive interactions. The established ANS-ICD method can provide valuable conformational insights into protein-ligand interactions in crowded cellular environments.

Determination of Hansen solubility parameters of water-soluble proteins using UV–vis spectrophotometry

Determination of solubility parameters by dissolution tests are difficult for some valuable molecules, such as proteins, where the quantities available are small, UV–vis spectroscopy can determine dissolved concentrations of even small amounts of material, but accurate determination of dissolution is difficult in relatively poor solvents, due to difficulty with constructing a reliable calibration curve. In this work we report a new simple procedure to determine the relative dissolution of proteins in various solvents using UV vis spectroscopy for the determination of Hansen solubility parameters (HSP) of proteins. This method allows qualitative determination of the amounts of BSA dissolved in various solvents. The amounts of BSA dissolved in each solvent, can then be used to rank solvents as good or bad for HSP calculation purpose, which gives more reliable ranking than observation alone in dissolution tests. To be able to evaluate the HSP of any solid material, the solubility of the tested material in a range of solvents needs to be determined. Solvents are then scored as good or bad based on observations and from the known properties of those solvents, the HSP of the solute molecule in question can be calculated. Here, bovine serum albumin (BSA) was used as a model protein, but the procedure reported here can be applied to any soluble protein or other macromolecule with a clear UV/vis adsorption peak. This procedure requires the tested material to be highly soluble in water, therefore eliminating the need for the preparation of many standard solutions of BSA in different solvents. Only a set of standard solutions of protein in water is required. UV–vis spectroscopy was used to analyze the remaining solid resuspended in water after centrifugation of solutions of BSA dissolved in other solvents to separate any undissolved protein. The HSP of BSA obtained via this procedure was compared to values previously obtained for BSA using other methods. A very good agreement between the HSP obtained in this work with the that reported by Houen et al. which used amino acid analysis for the estimation of the solubility of BSA in various organic solvents.

Single-detector double-beam modulation for high-sensitivity infrared spectroscopy

Balanced detection based on double beams is widely used to reduce common-mode noises, such as laser intensity fluctuation and irregular wavelength scanning, in absorption spectroscopy. However, employing an additional detector can increase the total system noise due to added non-negligible thermal noise of the detector, particularly with mid-infrared (IR) detectors. Herein, we demonstrate a new optical method based on double-beam modulation (DBM) that uses a single-element detector but keeps the advantage of double-beam balanced detection. The sample and reference path beams were modulated out-of-phase with each other at a high frequency, and their average and difference signals were measured by two lock-in amplifiers and converted into absorbance. DBM was coupled with our previously reported solvent absorption compensation (SAC) method to eliminate the IR absorption contribution of water in aqueous solutions. The DBM-SAC method enabled us to acquire IR absorption spectra of bovine serum albumin solutions down to 0.02 mg/mL. We investigated the noise characteristics of DBM measurements when the wavelength was either fixed or scanned. The results demonstrate that DBM can lower the limit of detection by ten times compared to the non-modulation method.

A comparison of surface properties for bovine serum albumin and human serum albumin – Dynamic/equilibrium surface tension and dilational modulus

The biological functionalities of Human Serum Albumin (HSA) and Bovine Serum Albumin (BSA) have been extensively studied in the literature. However, limited information is available on their surface properties [dynamic/equilibrium surface tension (ST) and dilational modulus (E)]. This study compared the dynamic/equilibrium ST of HSA and BSA solutions and the E of the adsorbed films. A pendant bubble tensiometer was employed for measuring the relaxations of ST of HSA/BSA solutions at C = 0.01 – 90 (10−10 mol/cm3). The data revealed that while the dynamic ST of HSA(aq) and BSA(aq) exhibited a similar relaxation in general, the ST of HSA(aq) relaxed notably faster at the post-induction regime than BSA(aq). The equilibrium ST of both HSA and BSA was constant at 52.6 and 52.3 mN/m over a wide concentration range, C = 0.01 – 90 (10−10 mol/cm3). The E of HSA and BSA films exhibited a near-identical concentration dependency to each other: E was reasonably constant at ∼ 21 mN/m from C = 0.05 to 0.2 (10−10 mol/cm3), and at further concentration elevations, E rose notably, and eventually leveled off at ∼ 52 and ∼ 45 mN/m for HSA and BSA films, respectively.