Glyphosate is largely used to control weeds in wetland soils of Brazil. We investigated changes in the chemistry of soluble manganese (Mn) and iron (Fe) in these soils as affected by glyphosate dosage. Triplicate samples of the A horizon of wetland soils with different organic-matter contents were incubated with deionized water (1:2) for 1, 3, and 30 days under flooding. Three different glyphosate doses (0, 0.048, and 0.096 g L−1 m−2) were spiked on the flooded water at the beginning of the incubation periods. After incubation, pH was measured and samples of the supernatant were collected for determination of Mn/Fe concentrations by atomic absorption. Glyphosate application impacted Mn but had no effect on pH and Fe. Soluble Mn concentrations decreased as glyphosate dosage increased for the high organic-matter soil after 3 days of incubation. It indicated that glyphosate application can change the chemistry of soil metals. The intensity of these changes depends on the glyphosate dosage, evolved metal, incubation time, and soil properties.