Soluble Fiber Content Research Articles

Effect of induced electric field treatment on structural and physicochemical properties of wheat bran to enhance soluble dietary fiber content.

Improving the content and physicochemical properties of soluble dietary fiber (SDF) in wheat bran (WB) is conducive to enhancing the palatability and processing adaptability of bran-containing products. In this study, induced electric field (IEF) was employed for the modification of WB. The IEF modification conditions were optimized, and the effects on the structural and physicochemical properties of WB and its SDF were evaluated. The results showed that under the excitation voltage of 500V, treatment time of 40min, and NaCl concentration of 0.1%, the SDF content in WB increased from 7.69% to 12.02%. The IEF-modified WB exhibited a porous structure and better hydration capabilities. Furthermore, the IEF modification resulted in a reduction in the crystallinity of WB, from 10.07% to 9.06%, and in the thermal stability, from 300.02℃ to 287.58℃ of the maximum decomposition. The SDF extracts study showed that IEF treatment destroyed the compact structure, reduced the thermal stability, and decreased the molecular weight and particle size from 1010nm to 227nm. In conclusion, IEF can effectively increase the SDF content and improve the quality of WB, which offers a theoretical reference for the comprehensive utilization of WB.

Impact of Cooking Techniques on the Dietary Fiber Profile in Selected Cruciferous Vegetable

Cruciferous vegetables of the plant order Brassicales are an attractive dietary component and a valuable source of fiber. However, the nutritional–physiological properties are different when comparing soluble and insoluble fibers. Another significant impact is the transformation of fibers by different influencing factors during food preparation. Cruciferous vegetables, especially, are dominantly processed to soften the matrix. As a result, during cooking, the polysaccharides are dissolved, swelled, or degraded to a certain extent, influencing the composition and the nutritional–physiological properties. The aim of the present study was to analyze the impact of different cooking procedures on changes in the dietary fiber content profile of three different plants: white cauliflower (Brassica oleracea L. var. botrytis), broccoli (B. oleracea L. var. italica), and Brussels sprouts (B. oleracea L. var. gemmifera). The sample material was subjected to direct (“in the water”) and steam cooking. The dietary fiber content and the content of its fractions were determined using an enzymatic analysis method. The results of the research show that the cooking process had a significant influence on the content of dietary fiber fractions in cruciferous vegetables. The concentration of insoluble dietary fiber decreased, whereas the content of soluble dietary fiber increased. When considering the average influence of each process, both steam cooking and direct cooking had a similar impact on changes in the concentrations of dietary fiber fractions. It can therefore be concluded that, when considering dietary fiber content, both processes can be equally well chosen as a thermal treatment for cruciferous vegetables.

Enhancing Rice Bran Soluble Dietary Fiber Yield Through Sequential Ultrasound–Xylanase Treatment

The main aim of this study was to enhance the content of soluble dietary fiber (SDF) derived from rice bran (RB) through various treatments, including physical methods (ultrasound and alternating magnetic field (AMF)) and enzymatic approaches (cellulase and xylanase), applied individually or in combination. The results revealed that AMF treatment was the most effective single modification technique for increasing SDF yield, followed by treatments with xylanase, cellulase, and ultrasound. Notably, among the combined approaches, the sequential ultrasound–xylanase treatment (U-X) demonstrated the highest potential for enhancing SDF yield. Further optimization experiments revealed that under the conditions of a xylanase addition of 4.3 mg/g sample, a material-to-liquid ratio of 50 mL/g, and an ultrasonic power of 72 W, the yield of U-X-SDF significantly increased from 1.03% to 18.4%. Compared to unmodified samples, the modified SDF groups exhibited marked enhancements in water holding capacity (42.5–86.4%) and water solubility (21.0–30.6%), while the unmodified SDF displayed superior oil holding capacity than the modified groups. In summary, the sequential ultrasound–xylanase treatment not only improves the SDF yield but also enhances the functional properties of RB-derived SDF, positioning it as a valuable health-promoting food additive with potential benefits for both laboratory and industrial food applications. The optimized treatment process can contribute to the development of new functional food ingredients from RB, thereby promoting health and wellness in consumers.

Enzymatic Modification of Apple Pomace and Its Application in Conjunction with Probiotics for Jelly Candy Production

This study aimed to evaluate the applicational possibilities of enzymatically modified apple pomace (AP) in conjunction with probiotics as value-added ingredients for the production of jelly candies. AP was enzymatically modified with Pectinex® Ultra Tropical, Viscozyme® L, and Celluclast® 1.5 L (Novozyme A/S, Bagsværd, Denmark), and the soluble and insoluble dietary fibre content was determined using the Megazyme kit (Megazyme International Ireland Ltd., Wicklow, Ireland), reducing sugar content using the 3,5-dinitrosalicylic acid assay. The technological properties of the modified AP, such as its swelling capacity, water-retention capacity, oil-retention capacity, bulk density, and static and thermal emulsion stability, were evaluated. Enzymatically modified AP hydrolysed with Celluclast® 1.5 L was used for the production of jelly candies supplemented with Bifidobacterium animalis DSM 20105. The survival of probiotics in the jelly candies during in vitro digestion, the viability of probiotics during candy storage, and candy quality characteristics were analysed. Enzymatically modified AP had different carbohydrate compositions and technological properties, depending on the enzyme preparation used. Although the viability of probiotics in the jelly candies decreased during storage, a significantly higher viability of B. animalis was determined in jelly candies supplemented with hydrolysed AP compared with control candies made without AP after digestion in the saline, gastric, and intestine phases. This study shows that Celluclast® 1.5 L can be used for increasing the soluble dietary fibre in AP (18.4%), which can be further applied, in conjunction with B. animalis, for added-value jelly candy production.

Effects of UV-A irradiation and microbial fermentation on the physicochemical, microstructure and functional properties of okara

Whole utilization of okara has important economic value, but there are two technical barriers: coarse mouthfeel caused by insoluble dietary fiber (IDF) and undesirable “beany” off-odors. UV-A irradiation and/or microbial fermentation were used to modify okara. The results indicated that single and combined treatments increased the soluble dietary fiber (SDF) content. Saccharomyces cerevisiae fermentation (YUO), Lactiplantibacillus plantarum fermentation (LUO), and mixed fermentation (MUO) followed by UV-A irradiation of okara significantly reduced the IDF/SDF ratio to 2.48, 1.86 and 2.25, respectively. The modifications significantly reduced the lipid and total nitrogen contents and decreased the E-nose sensor values associated with beany odors. The combined treatment of microbial fermentation and UV-A irradiation partially destroyed the crystalline, resulting in a loose and porous surface, further enhanced the functional properties of water holding capacity, water solubility, antioxidant properties and cation exchange capacity. In particular, the DPPH and ABTS scavenging abilities of okara subject to microbial fermentation followed by UV-A irradiation were greater than that of other samples. These results indicate that the treatment sequence is very important for the functional properties of okara and microbial fermentation followed by UV-A irradiation is most conducive to improve the physicochemical properties and functionalities of okara.

Millet bran dietary fibres treated by heating, dual enzymolysis united with acrylic-grafting or hydroxypropylation: effects on the properties of heat-induced egg white protein gel

IntroductionMillet bran is an abundant dietary fibre resource, but it is rarely used in Foods.MethodsTo enhance the functional properties and applications of foxtail millet bran dietary fibre (MBDF), MBDF modified by heating, cellulase and xylanase hydrolysis combined with acrylic-grafting (MBDF-HDEAG) or hydroxypropylation (MBDF-HDEH) were prepared, and the effects of these modified MBDFs on heat-induced egg white protein gel (H-EWPG) were studies.Results and discussionThe results showed that heating and enzymolysis united with acrylic-grafting or hydroxypropylation both enhanced the surface area, soluble fibre content, water-retention and expansion abilities of MBDF. The addition of unmodified MBDF, MBDF-HDEAG and MBDF-HDEH increased the β-sheet content of H-EWPG and made its microstructure denser and granular. Compared with MBDF, MBDF-HDEAG and MBDF-HDEH more effectively improved the gel and texture properties of H-EWPG including water-holding ability (from 20.45 to 34.63 g/100 g), pH (from 4.53 to 7.66), hardness (from 63.92 to 104.53 g), chewiness (from 57.97 to 122.84 g), and gumminess (from 63.92 to 118.18), and a reduction in transparency and springiness (p < 0.05). MBDF showed the highest reducing effect on the freeze-thaw dehydration of H-EWPG (from 39.02 to 21.62%). Therefore, addition of MBDFs modified by heating, enzymolysis united with acrylic-grafting or hydroxypropylation can improve gel properties of H-EWPG.

Effects of Modified Oil Palm Kernel Expeller Fiber Enhanced via Enzymolysis Combined with Hydroxypropylation or Crosslinking on the Properties of Heat-Induced Egg White Protein Gel.

Due to its poor hydration properties, oil palm kernel expeller dietary fiber (OPKEDF) is rarely used in the food industry, especially in hydrogels, despite its advantages of high availability and low cost. To address this situation, the effects of enzymolysis combined with hydroxypropylation or crosslinking on the structure and hydration properties of OPKEDF were investigated, and the impact of these modified OPKEDFs on the properties of egg white protein gel (EWPG) was studied. Enzymolysis combined with hydroxypropylation or phosphate crosslinking improved the soluble fiber content (5.25-7.79 g/100 g), water-retention and expansion abilities of OPKEDF (p < 0.05). The addition of unmodified OPKEDF or modified OPKEDF increased the random coil content of EWPG and increased the density of its microstructure. Moreover, enzymolysis combined with hydroxypropylation or crosslinking enhanced the effect of OPKEDF on the properties of EWPG, including improvements in its water-retention ability, pH, hardness (from 97.96 to 195.00 g), chewiness (from 78.65 to 147.39 g), and gumminess (from 84.63 to 152.27) and a reduction in its transparency (p < 0.05). Additionally, OPKEDF and enzymolysis and hydroxypropylated OPKEDF increased the resilience (0.297 to 0.359), but OPKEDF treated via enzymolysis and crosslinking reduced it. Therefore, OPKEDF modified by means of enzymolysis in combination with hydroxypropylation or crosslinking improved the gel properties of EWPG. However, further work is required to determine the effects of these modifications on the nutritional profile, scalability, and economic feasibility of OPKEDF and egg white gel.

Effects of combining ultrasound and dual enzymolysis with hydroxypropylation, acrylate grafting, or phosphate grafting on the physicochemical and functional properties of oil palm mesocarp expeller dietary fiber

Oil palm mesocarp expeller is an abundant and low-cost dietary fiber resource, but its poor hydration and adsorption properties limit its utilization in foods. Therefore, ultrasound and enzymolysis combined with hydroxypropylation, acrylate grafting, or phosphate grafting were used to improve the adsorption properties of oil palm mesocarp expeller fiber (OPMEDF) for the first time. The results revealed that these composite modifications made the microstructure of OPMEDF more porous, and significantly increased its soluble fiber content, surface area, hydration properties, and sorption abilities of glucose, nitrite, copper, and lead ions but decreased its brightness. OPMEDF modified by ultrasound, enzymolysis, and acrylate grafting had the highest content of extractable polyphenol and sorption abilities on oil and nitrite ion. OPMEDF subjected to ultrasound, enzymolysis, and phosphate grafting showed the highest water-swelling volume (3.84 mL g−1) and sorption abilities of copper (II) and lead (II) ions. Notably, OPMEDF modified by ultrasound, enzymolysis, and hydroxypropylation exhibited the highest soluble fiber content (14.72 g∙100 g−1), water-retention ability (5.22 g g−1), viscosity (13.90 cp), and glucose adsorption capacity (38.18 g∙100 g−1). These results indicated that these composite modifications are good options to improve the adsorption capacities of OPMEDF and can expand its potential hypoglycemic and hypolipidemic effects.

Influences of superfine-grinding and mix enzymolysis alone or combined with hydroxypropylation or acetylation on the hypolipidemic and hypoglycemic properties of coconut endosperm residue fiber.

Coconut endosperm residue is an abundant and low-cost resource of dietary fiber, but the low soluble fiber content limits its functional properties and applications in the food industry. To improve the hypolipidemic and hypoglycemic properties, coconut endosperm residue fiber (CERF) was modified by superfine-grinding and mix enzymatic hydrolysis alone, or combined with acetylation or hydroxypropylation. The effects of these modifications on the structure and functional properties were studied using scanning electron microscopy, Fourier-transformed infrared spectroscopy, and in vitro tests. After these modifications, the microstructure of CERF became more porous, and its soluble fiber content, surface area, water adsorption, and expansion capacities were all improved (p < 0.05). Moreover, superfine-grinding and mix enzymolysis combined with acetylation treated CERF showed the highest surface hydrophobicity (48.96) and cholesterol and cholate adsorption abilities (33.72 and 42.04mg∙g‒1). Superfine-grinding-, mix enzymolysis-, and hydroxypropylation-treated CERF exhibited the highest viscosity (17.84 cP), glucose adsorption capacity (29.61µmol∙g‒1), and glucose diffusion inhibition activity (73.96%), and water-expansion ability (8.60mL∙g‒1). Additionally, superfine-grinding and mix enzymatic hydrolyzed CERF had the highest α-amylase inhibiting activity (42.76%). Therefore, superfine-grinding and mix enzymolysis alone or combined with hydroxypropylation were better choices to improve hypoglycemic properties of CERF; meanwhile, superfine-grinding and mix enzymolysis combined with acetylation can effectively improve its hypolipidemic properties. PRACTICAL APPLICATION: This study offered three composite modification methods to improve the soluble fiber content and in vitro hypolipidemic and hypoglycemic properties of coconut endosperm residue fiber. These modification methods were practicable and low-cost. Moreover, it provides good choices to improve the functional properties and applications of other dietary fibers in the food industry.

Effect of spray-drying or fermentation on the solubility and carbohydrate profile of chickpea hydrolysates for beverage formulation

Powder beverage bases were developed using extruded and hydrolyzed chickpea supernatant through freeze-drying. This study evaluated the effects of spray-drying and adjuvant incorporation for producing powders and supernatant fermentation with LAB strains prior freeze-drying on the carbohydrate composition, structural integrity, and physical properties of chickpea beverage base powders. All powders had low insoluble (<0.2 %) and soluble (<3 %) dietary fiber contents due to processing. Starch content decreased below 9.49 % when using adjuvants and 10.61 % during fermentation. Both treatments also reduce raffinose oligosaccharide content below 6.1 mM/100 g. Spray-drying and adjuvant addition promoted a more amorphous starch structure, enhancing solubility, while fermentation induced more crystallinity, according to FTIR data. Microscopy showed small particle clusters and lack of birefringence. SEM images revealed starch granule damage from both fermentation and spray-drying, with more uniform particles in spray-dried powders. Spray-drying resulted in the smallest particle sizes (455-457 nm) and higher absolute zeta potential (8.12-9.51), indicating greater stability. Fermentation had the opposite effect, negatively impacting the colloidal system stability. Samples had a pH close to 6, except fermented powders with a pH of 3.6, affecting colloidal stability. Fermentation significantly reduced solubility but without practical implications, as all samples had solubility values over 93 %, which is desirable for beverage-based powders. Spray-drying and fermentation altered the carbohydrate profile and structure of the powders. Fermentation prior producing powders may provide benefits, but it slightly decreases the system stability. Spray-drying can be used instead of freeze-drying to produce stable chickpea base powders that are highly soluble.

Enhancement of Corn Flour with Carob Bean for Innovative Gluten-Free Extruded Products.

The aim of this work is to study new, extruded products based on corn flour enriched with carob bean and the evaluation of its functional quality to develop novel gluten-free food products. Five samples based on corn flour with added carob bean flour (5 to 12.5%) were formulated. Extrusion was performed using a single-screw laboratory extruder at pilot plant scale. Extrusion parameters such as color and carbohydrate content (fiber, sucrose, and starch) were evaluated. Carob bean addition led to an increase in starch, soluble fiber, and insoluble fiber. Texture parameters related to hardness (crunchiness) were significantly reduced with the addition of CB (p < 0.05), detectable from a 5% addition of CB and not significant with more CB content. Samples became browner with the addition of CB; however, when the concentrations of CB are high (>5%) no major differences in color were observed. The extrusion process reduced the content of soluble and insoluble fiber, and sucrose in all formulated samples. Extruded samples with 5-7.5% CB seem to be the best formulation in terms of fiber content, color, and texture parameters. These innovative gluten-free foods could be considered as a source of fiber, and a healthier alternative to some commercially available snacks.

Effect of extrusion on physicochemical and functional properties of cladode flour from Opuntia cochenillifera and Opuntia ficus-indica

In this study, the physicochemical and functional properties of flours obtained from mature cladodes of two nopal species, Opuntia cochenillifera (OC) and Opuntia ficus indica (OF), subjected to different extrusion conditions, varying moisture (20 % and 26 %) and temperature levels (120 °C, 140 °C and 160 °C) were evaluated. The results showed significant differences in chemical composition, physical, functional and phytochemical properties between the two species. OC had a higher ash and ether extract content (29.27 % and 1.28 % respectively), while OF had more protein and carbohydrates (7.83 and 55.31 % respectively). The expansion index was higher (1.06 – 1.27) in OF, especially under high temperature extrusion and low moisture conditions. Bulk density decreased with increasing extrusion temperature, and color was significantly altered by temperature and moisture, with greater changes observed in OF. Functional properties such as oil absorption capacity, water absorption capacity, and water solubility index, were significantly affected by the extrusion process in both species of nopal. In terms of dietary fiber, extrusion increased (4.99 to 10.38 %) the soluble fiber content and decreased (36.91 to 32.30 %) the insoluble fiber content in both species. However, OC had a higher percentage of soluble and total dietary fiber. Phenolic compounds, flavonoids and antioxidant activity were significantly affected by the extrusion process in most treatments, although some maintained or improved these properties. These results highlight the influence of species and extrusion conditions on the properties of nopal flours, providing valuable information for the development of nopal-enriched food products, thereby optimizing their nutritional and technological properties.

Solid-State Fermentation of Wheat Bran with Clostridium butyricum: Impact on Microstructure, Nutrient Release, Antioxidant Capacity, and Alleviation of Ulcerative Colitis in Mice.

This study investigated the effects of solid-state fermentation with Clostridium butyricum on the microstructure of wheat bran, the release of dietary fiber and phenolic compounds, and antioxidant capacity. Compared with unfermented wheat bran, insoluble dietary fiber and phytic acid content decreased, whereas soluble dietary fiber and water-extractable arabinoxylan content increased in C. butyricum culture. Because of the increased release of phenolic compounds, such as ferulic acid and apigenin, and organic acids, such as isobutyric acid, the antioxidant capacity of the culture was considerably improved. Furthermore, the culture of C. butyricum treated with dextran sulfate sodium-induced ulcerative colitis in mice enhanced the expression of intestinal mucus and tight-junction proteins, modulating the gut microbiota structure, increasing the levels of short-chain fatty acids in the intestine, and restoring the essential functions of the gut microbiota. These anti-inflammatory effects stemmed from the combined action of various effective components.

Mechanisms of Degradation of Insoluble Dietary Fiber from Coconut Chips by Ultra-High Pressure

Coconut chips are a popular leisure food, but the residual crumbly feeling after chewing affects the eating experience. To address this problem, we investigated the mechanism of degradation of insoluble dietary fiber (IDF) from coconut chips by ultra-high pressure (UHP). The optimal conditions for UHP treatment were 100 MPa and 40 min. After UHP treatment, the hardness decreased by 60%, and the content of soluble dietary fiber (SDF) increased by 55%. So far, the meaning of SDF has not been defined. The microstructure of IDF was damaged and the surface was rough. There was no obvious change in the chemical structure. The position of the characteristic diffraction peaks was basically unchanged, but the crystallinity dropped by almost three times. The thermal stability decreased, and the composition of the monosaccharides changed. Together, UHP treatment can improve the problem of the residual crumbly feeling after chewing coconut chips and improve the quality of the product.

Effect of superfine grinding mulberry leaves (Morus alba Linn.) powder on biscuit properties

SummarySuperfine and regular grinding methods were used to mill mulberry leaves (ML), and the chemical composition and physical characteristics of the powders were investigated. Compared to regular grinding, superfine grinding reduced particle size from 121.44 μm to 18.69 μm. Compared to coarse mulberry leaf (CML), the fine mulberry leaf (FML) powder had a greater water solubility index, soluble dietary fibre content and 1‐deoxynojirimycin (DNJ) content. Both types of ML powders were substituted for flour at various percentages (3%, 6%, 9% and 12%), and their effects on the biscuits' textural qualities and sensory assessment were examined. The adhesiveness, cohesiveness and springiness of biscuit doughs were all reduced by ML powder addition, while their hardness was raised. The inclusion of ML powders increased the hardness of the biscuits significantly, whereas only the addition of FML powders decreased their fracturability. Additionally, the DNJ contents in FML biscuits were significantly higher than that in CML biscuits. The sensory assessment revealed that biscuits containing 9% FML powder received the best rating.

Effects of cantaloupe rind powder fortification on yogurt properties: Comparison between raw and enzyme-treated powder

This study investigated the potential of fortifying a probiotic yogurt with cantaloupe rind powder. The raw cantaloupe rind powder was subjected to enzyme treatment to enhance its soluble fiber content. The effects of incorporating raw and enzyme-treated cantaloupe rind powder (referred as RCRP and ECRP, respectively) on the physicochemical properties, textural and rheological properties, antioxidant properties and syneresis of yogurt were evaluated during a 15-day storage period at 4 °C. The results revealed that the inclusion of RCRP resulted in a reduction in yogurt hardness, viscosity, elasticity and stability compared to the control yogurt without cantaloupe rind powder fortification. In contrast, adding ECRP led to an increase in the hardness, viscosity, elasticity and stability of the yogurt. Specifically, on day 15 of storage, the syneresis of ECRP-fortified yogurt was approximately 3 times lower, while that value of RCRP-fortified yogurt was approximately 2 times higher compared to the control yogurt. Notably, the RCRP- and ECRP-fortified yogurts displayed 14- and 9-times higher antioxidant activity, respectively, compared to the control yogurt. This study suggests that employing enzyme treatment on cantaloupe rind powder holds promise as an approach to yield positive effects when incorporating the powder into yogurt, particularly in terms of stability, textural and rheological properties.

Comparative effect of FYM and Azotobacter on morphology and nutritional quality of high and low altitude grown knol khol (Brassica oleracea var. gongylodes L.) cultivar White Vienna

Extreme environmental conditions of high altitude (HA) expose the food crops to abiotic stresses that may impact their growth and nutritional composition, which also varies depending on agricultural practices being used. Therefore, the present study is designed to a compare the efficacy of organic agri-treatments viz. farm yard manure (FYM) and Azotobacter on morphological and nutritional traits of knol-khol cultivar White Vienna grown at HA and low altitude (LA). The field trial was conducted as a two-factorial experiment in a randomized block design. The first factor was treatments (T1-FYM, T2-Azotobacter, T3-FYM+Azotobacter and T4-(control) and second factor was cultivation locations (HA vs. LA). The findings revealed that the application of treatment T3 at HA resulted in higher total soluble solids (1.38-fold), titratable acidity (0.06-fold), total carbohydrate (1.9-fold), crude protein (3.7-fold), crude fat (3-fold) and dietary fiber content (78-fold) whereas, yield (137.6-fold) and ash content (0.85-fold) content were found higher at LA. The current study emphasizes upon the superior efficiency of combination treatment of FYM and Azotobacter at HA to improve the nutritional quality of food crops, with added benefits of environmental sustainability and nutritional security.

Effect of Physical Separation with Ultrasound Application on Brewers' Spent Grain to Obtain Powders for Potential Application in Foodstuffs.

Brewers' spent grain (BSG) is the primary by-product of beer production, and its potential use in food products is largely dependent on its processing, given its moisture content of up to 80%. This study aimed to evaluate the effects of physical separation with ultrasound application on the color, total phenolic content (TPC), antioxidant activity, proximate composition, total dietary fibers, and particle size distribution of BSG powders. Wet BSG (W) was subjected to two processes: one without ultrasound (A) and one with ultrasound (B). Both processes included pressing, convective air-drying, sieving, fraction separation (A1 and B1 as coarse with particles ≥ 2.36 mm; A2 and B2 as fine with particles < 2.36 mm), and milling. The total color difference compared to W increased through both processes, ranging from 1.1 (B1 vs. A1) to 5.7 (B1 vs. A2). There was no significant difference in TPC, but process B powders, particularly B2, showed lower antioxidant activity against ABTS•+, likely due to the release of antioxidant compounds into the liquid fraction during pressing after ultrasound treatment. Nonetheless, process B powders exhibited a higher content of soluble dietary fibers. In conclusion, ultrasound application shows potential for further extraction of soluble fibers. However, process A might be more practical for industrial and craft brewers. Further studies on the use of the resulting BSG powders as food ingredients are recommended.

Millet Bran Dietary Fibers Modified by Heating and Enzymolysis Combined with Carboxymethylation, Acetylation, or Crosslinking: Influences on Properties of Heat-Induced Egg White Protein Gel.

Applications of millet bran dietary fiber (MBDF) in the food industry are limited by its poor hydration properties. Herein, MBDF was modified by heating, xylanase and cellulase treatment separately combined with carboxymethylation, acetylation, and phosphate crosslinking, and the effects of the modified MBDFs on heat-induced egg white protein gel (H-EWG) were studied. The results showed that three composite modifications, especially heating and dual enzymolysis combined with carboxymethylation, increased the surface area, soluble fiber content, and hydration properties of MBDF (p < 0.05). MBDF and the modified MBDFs all made the microstructure of H-EWG denser and decreased its α-helix content. Three composite modifications, especially heating and dual enzymolysis combined with carboxymethylation, enhanced the improving effect of MBDF on the WRA (from 24.89 to 35.53 g/g), pH, hardness (from 139.93 to 323.20 g), chewiness, and gumminess of H-EWPG, and enhanced the gastric stability at 3-5 g/100 g. MBDFs modified with heating and dual enzymolysis combined with acetylation or crosslinking were more effective in increasing the antioxidant activity of the gastrointestinal hydrolysates of H-EWG than MBDF (p < 0.05). Overall, heating, xylanase and cellulase treatment separately combined with carboxymethylation, acetylation and crosslinking can enhance the hydration properties and the improving effect of millet bran fibers on H-EWG properties.

Development of technology for functional prebiotic fondant candies using powder made of jerusalem artichoke pulp

The main disadvantage of most fondant candies is high sugar content and calorie content, low content of useful essential nutrients, rapid drying and staling during storage. It is relevant to use jerusalem artichoke pulp powder instead of sugar, which contains a significant amount of dietary fiber and minerals with high moisture-retaining properties. The introduction of jerusalem artichoke pulp powder in an amount from 5 to 9% contributes to: acceleration of the structure formation of the fondant mass due to the intensification of the sucrose crystallization process; to change the rheological properties of the fondant, namely, to increase its viscosity and plastic strength; to increase the dispersion of the fondant, while the proportion of sucrose crystals less than 20 microns in size in the fondant increases by 9–16%; to reduce the drying process of sweets during storage; to reduce sugar content, calorie content and increase nutritional value by increasing the content of dietary fibers and mineral substances. When using jerusalem artichoke pulp powder in an amount of 9% instead of sugar, it allowed to obtain functional fondant candies in terms of the content of soluble dietary fiber (31.5% of the daily requirement), which have a prebiotic effect. Compared with the control sample, the carbohydrate content in the experimental sample decreased by 8.1%, the calorie content decreased by 27 kcal, the potassium content increased by 42.9 times, calcium by 3.5 times, magnesium by 4 times, phosphorus by 12 times, iron by 8 times. Therefore, the use of this concentrator is advisable and promising from the point of view of improving the quality and increasing the nutritional value of the product, the developed technology of fondant candies using jerusalem artichoke pulp powder can be recommended for implementation in confectionery enterprises.