Soldier Fly Research Articles

Antibacterial peptides from black soldier fly (Hermetia illucens) larvae: mode of action and characterization

Antibacterial peptides from black soldier fly larvae extract were prepared using Flash column chromatography. Three out of five fractions (F2, F3 and F4) showed antibacterial activity against Listeria monocytogenes DMST 17303 with a minimum inhibitory concentration (MIC) of 1 mM, followed by Salmonella enterica Enteritidis DMST 15679 and Escherichia coli O157:H7 DMST 12743 with a MIC ranging from 4 to 8 mM. Due to the higher yield, F2 and F3 were further analyzed on their mode of action against L. monocytogenes DMST 17303. Both fractions, particularly F2, exerted antibacterial activity through inducing bacterial cell membrane disintegration and interaction with intracellular compounds including fatty acids, proteins, and nucleic acids. The F3 did not show significant hemolytic activity up to 4 mM, while F2 showed lower than 5% hemolysis up to 8 mM. Time-to-kill analysis revealed that F2 was more effective and exerted a sustainable killing effect after 2 and 4 h, depending on the concentration of 1 and 2×MIC, respectively, while the F3 at 2×MIC could completely kill the test bacteria within 24 h. Among the identified peptides in the fractions, those with charged, either positively or negatively, and moderate hydrophobicity of ranging 6.68–15.70, namely CGPPRQGPFPR, HLEEELK, LEEAEERAD, TEELEEAKKK, and KGNSELEEAKKK, are potential antimicrobial peptides.

Nutritional composition of black soldier fly (Hermetia ilucens) larvae reared on vegetable wastes: effects on growth of village chickens

Context Black soldier fly larvae are a promising alternative ingredient in poultry feed. They might be able to replace soybean and fishmeal because the have high concentrations of protein and fat, and are also rich in vitamins and minerals, and because partial replacement of soybean and total replacement of fishmeal produce no adverse effect on the growth performance of village chickens. Therefore, they are a suitable replacement for these traditional ingredients in poultry feed. Aims This study determines the nutritive values of black soldier fly larvae and evaluates the effects of feeding diets containing black soldier fly larvae on the growth performance of village chickens. Methods Samples of black soldier fly larvae were subjected to proximate analysis before a total of 280 1-day-old village chicks were randomly divided into four groups. All groups were fed a basal diet with partial replacements of corn and soybean with black soldier fly larvae at 0% (Control), 5% (T1), 10% (T2) and 15% (T3) for 70 days. Each treatment group consisted of seven replicates, with 10 birds per replicate. The bodyweights, bodyweight gains and feed conversion rates were determined. Key results Dry rendered black soldier fly larvae contained 773.0 ± 0.08 g/kg dry matter, 36.1 ± 0.09 g/kg ash, 408.8 ± 0.28 g/kg crude protein, 283.0 ± 0.16 g/kg ether extract and 40.9 ± 0.44 g/kg crude fibre. The larvae also contained 2.041 MJ/kg of metabolisable energy, 20.4 g/kg of calcium and adequate concentrations of vitamins. The feeding trial showed an average bodyweight gain of 1231.45 ± 23.37 g and feed conversion ratio of 2.03 ± 0.15, which were most efficient with Diet T3. Conclusions Inclusion of 15% black soldier fly larvae in diets can enhance the growth performance of village chickens without adverse effects. Implications Partial replacement of soybean and total replacement of fishmeal in the diet of chickens by black soldier fly larvae are not affecting the growth performance of village chickens but might reduce the feed cost.

Effect of Black Soldier Fly Larvae Frass as Organic Fertilizer on Postharvest Quality and Shelf Life of Open-field-grown Tomato (Solanum lycopersicon L.)

Open-field-grown tomatoes in Saudi Arabia are stressed by poor soil fertility because of the low organic matter content, low nutrient availability, and high pH. Thus, high chemical fertilizer inputs are crucial for commercial production; however, they cause economic loss as well as negatively affect environmental sustainability. The use of efficient organic fertilizers would be useful for developing more cost-effective, environmentally friendly, and sustainable agriculture. The current study evaluated the impact of Black soldier fly (Hermetia illucens L.) frass (BSFF) fertilizer applications as organic fertilizer on postharvest fruit quality and shelf life of open-field-grown F1 hybrid ‘Sinag Tala’ tomatoes compared with those grown under control treatment (received recommended doses of chemical fertilizers for tomato production under open field conditions). The experiment was conducted using a randomized complete block design with four replicates. The results showed that the application of BSFF fertilizer, especially at the higher rate (5 ton/ha; BSFF3), improved the overall quality of tomato fruit. The BSFF fertilizer treatments reduced weight loss, maintained higher firmness, antioxidants [vitamin C, total phenol content (TPC) and total flavinoid (TFC)], total soluble solids (TSS), and titratable acidity (TA) contents, and lowered the TSS/TA ratio of fruits during shelf life compared with those of the control. The decay incidence was only detected after 9 days of shelf life and was significantly lower in all the BSFF fertilizer treatments (range, 8.40%-12.05%) than in the control (15.9%). In addition, BSFF fertilizer treatments reduced both disease incidence and severity of gray mold following pathogen inoculation during shelf life compared with those of the control. These results might be attributable to the higher antioxidants content (vitamin C, TPC, and TFC) and higher polyphenol oxidase activity in BSFF-fertilized tomatoes. In conclusion, BSFF fertilizer could be used as a potential eco-friendly alternative to chemical fertilizers to improve tomato fruit quality during shelf life.

Black soldier fly larvae (Hermetia illucens) do not bioaccumulate ferulic and caffeic acids from wheat bran

Black soldier fly larvae (Hermetia illucens) do not bioaccumulate ferulic and caffeic acids from wheat bran

Effect of temperature on growth, metabolism, and gas exchange in Hermetia illucens larvae reared under commercial and laboratory conditions

Abstract The world’s socio-economic development is continuously increasing the demand for efficient production of food, feed, and energy from the agricultural sector. In this respect, the emerging production of black soldier fly larvae (BSFL) represents a promising system to upscale low-quality resources to higher-quality resources usable as feed or biodiesel. To optimize BSFL production it is critical to establish methods that are relevant to examine how rearing conditions, including temperature, affect growth, metabolism, and body composition at large scale. In a first set of experiments, we therefore compared how small-scale laboratory respirometry chambers (600 larvae) support gas exchange, growth, and metabolism in BSFL with similar measurements obtained from large-scale commercial conditions (>12 million larvae) at ∼34-35 °C. Having observed that small- and large-scale systems generated comparable measurements of growth and metabolism, we used our small-scale respirometry system to examine in detail how substrate rearing temperature ranging from 27.0 to 41.9 °C influenced growth, metabolism, and body composition in BSFL. Here we found that 7-day growth of BSFL was stable and high at 27, 35, and 39 °C, while a rearing temperature of ∼42 °C caused a severe, but sublethal, depression of growth and metabolism. Despite the general similarities in total growth at 27, 35, and 39 °C, we found considerable acceleration of metabolism and development at the two higher rearing temperatures (35 and 39 °C). In contrast, the lower rearing temperature (27 °C) resulted in reduced CO2 production per kg larvae produced and the was associated with a higher crude protein to crude lipid ratio in larvae after the 7-day growth period. Based on these findings we discuss how continued monitoring of gas exchange in production systems holds a potential to optimize production in the emerging industry of large-scale commercial insect production.

Partial or complete replacement of soybean meal with black soldier fly larvae meal improves feed efficiency in laying hens between 22 to 30 weeks of age

Abstract The European Commission recently authorised the inclusion of insect meal in poultry feed. Black soldier fly larvae meal (BSF) has comparable nutritional value to soybean meal (SBM) and higher calcium content, making it an attractive alternative protein source for laying hens While a few studies have explored this objective, inconsistent results have been reported, likely due to variations in hen age, breed, husbandry systems, and sunflower meal composition across studies, making it difficult to draw clear conclusions. We hypothesised that partial or complete replacement of SBM with BSF has no adverse effects on egg production and quality in laying hens at the initiation of laying (22-30 weeks of age). This study consisted of three dietary treatments and nine replicates per treatment. Diets were a control diet, and two diets containing 5% (BSF5%) and 10% (BSF10%). The experimental unit was a pen (100 × 200 × 200 cm) of 14 Brown Nick laying hens resembling an aviary setting. We found that complete replacement of SBM with BSF10% in the diet of laying hens improved feed efficiency attributed to a lower feed intake in comparison to laying hens fed the BSF5% or the control diets, while production performance, body weight, and egg quality were maintained and the colour of the egg yolk increased. Therefore, replacing SBM with up to 10% BSF in brown laying hens in aviary pens at the initiation of laying had no adverse effects on production performance, chemical and physical characteristics, and sensory attributes of eggs. Maintaining production and egg quality with a lower intake of nutrients requires further investigation.

Dietary full-fat or defatted black soldier fly larvae can replace protein sources with no detrimental effect on growth performance or intestinal health of nursery pigs.

This work aimed to determine the effects of dietary full-fat or defatted black soldier fly larvae (BSFL) to replace protein sources on growth performance, blood parameters, intestinal morphology, and intestinal microbiota in nursery pigs and to investigate the effects of dietary defatted BSFL at up to 30% at the expense of protein sources on growth performance in nursery pigs. In Exp. 1, a total of 36 barrows with an initial body weight of 7.0kg (standard deviation = 0.8) were allotted to 3 dietary treatments in a randomized complete block design with 4 replicate pens per treatment and 3 barrows per pen. A corn-soybean meal (SBM)-whey-based control diet was prepared with soy protein concentrate and fish meal as additional protein supplements. Two additional diets were prepared to include 20% full-fat BSFL or 20% defatted BSFL to replace soy protein concentrate and fish meal to maintain the same energy and nutrient concentrations in all diets. In the 28-day feeding trial, pigs fed the diet containing defatted BSFL tended to consume more feeds (P < 0.10) than other groups during days 14 to 28 and the overall period. On day 28, the serum blood urea nitrogen in pigs fed the control diet was less (P < 0.05) than that fed the full-fat or defatted BSFL, but fecal score and jejunal morphology did not differ among the treatment groups. Relative abundance of Mycoplasma in the ileal digesta was less (P < 0.05) in the pigs fed the diet containing full-fat or defatted BSFL compared with the control group. In Exp. 2, a total of 192 pigs with an initial body weight of 7.8 (standard deviation = 1.2kg) were randomly allotted to one of 4 dietary treatments in a randomized complete block design with 6 replicate pens per treatment and 4 barrows and 4 gilts per pen. A control diet was mainly based on corn, SBM, fermented SBM, fish meal, and spray-dried plasma protein. Three additional diets were prepared to contain 10%, 20%, and 30% defatted BSFL to replace SBM, fermented SBM, fish meal, and spray-dried plasma protein to maintain for the same energy and nutrient concentrations. Average daily gain, average daily feed intake, gain:feed, and fecal score were not affected by increasing dietary defatted BSFL. Overall, dietary black soldier fly larvae did not compromise growth performance or intestinal health in nursery pigs. Black soldier fly larvae can be used in nursery pig diets to replace other protein sources without negative effects.

Dielectric drying of black soldier fly larvae (Hermetia illucens): impact on microbiological product quality, safety and stability

Abstract Along with the increasing scale of industrial production of insects as food and feed, the need for processing methods applicable on large scale is rising within the insect sector. One important processing technology in this context is drying, which aims at stabilising the quality of the insects. This study investigated the impact of dielectric drying technologies, specifically microwave (μW) and radio frequency (RF) drying, on the microbiological quality, safety, and stability of black soldier fly (BSF) larvae in comparison to the traditional methods oven drying and freeze drying. Before, during and after subjecting a large batch of blanched BSF larvae to the four drying technologies, their water activity and moisture content as well as general microbial and pathogen counts were assessed. Dielectric drying effectively reduced water activity, thus enhancing product stability. Microbiological analyses revealed the efficacy of blanching in microbial reduction, with dielectric (especially RF) drying further contributing to these reductions. The results, however, do not conclusively support nonthermal microbial inactivation through dielectric treatment. Specific analyses targeting foodborne pathogens indicated the absence of most selected bacterial pathogens, except for (presumptive) Bacillus cereus. This pathogen was detected in a few samples but remained below safety thresholds and was effectively eliminated by dielectric drying. Finally, a shelf life stability evaluation showed minimal changes in dielectrically dried BSF larvae over six months, indicating a microbiologically stable product. In conclusion, dielectric drying technologies can retain or even improve the microbiological quality and stability of BSF larvae in the insect industry and can be considered as valuable and more efficient (time, energy consumption) alternatives to oven and freeze drying.

Evaluating Black Soldier Fly and Bloodworm Larvae Meals as Sustainable Protein Sources for Growth and Survival of Red Hybrid Tilapia (Oreochromis spp.) in the Nursery Phase

Aims: This study aimed to assess the effects of using insect larvae meals, such as BSF and BW larvae, as alternative feeds to commercial pellets on the growth performance and survival of Oreochromis spp. at the nursery stage. Study Design: This study followed the randomized control treatment with three different diet. Place and Duration of Study: This study was carried out at the Fisheries Research Institute Glami Lemi (FRIGL) in Negeri Sembilan, Malaysia, within 35 days from July to August 2023. Methodology: A total of 360 red hybrid tilapia (Oreochromis spp.) fingerlings weighing 0.62 ± 0.06 g were randomly distributed into 12 rectangular aquarium tanks (250mm) at a stocking density of 30 fish per tank. They were fed with commercial pellets (CP), Black Soldier Fly (BSF) larvae, and Bloodworm (BW). The feed amount was calculated at 10% of the total body weight per tank, with feeding conducted three times daily. The experimental setup was performed in triplicate. For the analysis of growth performances and fish survival, sampling was conducted weekly. Results: BSF larvae meal had high lipid and protein content, measuring 35.35 ± 0.21% and 29.67 ± 0.98%, respectively. BW larvae meal also showed high lipid and protein content, with 28.23 ± 1.33% and 27.94 ± 0.52%, respectively. Fish fed with BSF and BW larvae exhibited significantly higher survival rates, 93.3 ± 3.3% and 86.7 ± 14.5%, respectively, compared to the control group. Meanwhile, the group fed with the commercial pellet showed the lowest survival rate, just 74.4 ± 12.6%. Fish fed with the BW larvae recorded the highest weight gain, 1.07 ± 0.16 g, followed by fish fed with BSF larvae, 0.66 ± 0.11 g, and then fish fed with commercial pellets, 0.41 ± 0.09 g. Conclusion: Both BSF and BW larvae exhibited significant growth response properties to tilapia fingerings compared to commercial pellets. The findings from this study are promising and should be further verified at the farm scale.

Effects of Black Soldier Fly Larvae Oil on Growth Performance, Blood Biochemical Parameters, Carcass Quality, and Metabolomics Profile of Breast Muscle of Thai Native Chickens

This study aimed to evaluate the effects of the replacement of rice bran oil (RBO) with black soldier fly larvae oil (BSFLO) on growth performance, blood biochemicals, carcass quality, and metabolomics profile of breast muscle of Thai native chickens. A total of 192 1-day-old, mixed-sex, Pradu Hang Dam (Mor Kor 55) chickens were randomly allocated to one of three dietary groups. Each treatment had four replicates with 16 chicks per replicate (8 males and 8 females). Three dietary treatments were used: (T1) the control group, based on a corn–soybean meal with RBO, and two treatment groups that replaced 50% (T2) and 75% (T3) of RBO in the basal diet with BSFLO, respectively. Results showed that BSFLO inclusion at 50% and 75% did not adversely affect the productive performance of Thai native chickens (p &gt; 0.05). Regarding blood profiles, on day 28, chickens fed 75% BSFLO exhibited significant increases in hemoglobin, hematocrit, and MCHC (mean corpuscular hemoglobin concentration) with lower eosinophil percentages compared to the control group (p &lt; 0.05). Additionally, BSFLO supplementation raised glucose levels but decreased globulin and total protein levels (p &lt; 0.05). On day 63, BSFLO inclusion primarily affected MCV (mean corpuscular volume), with higher values in the 50% BSFLO group (p &lt; 0.05). It also increased globulin and HDL (high-density lipoprotein) levels while lowering AST (aspartate transaminase) concentrations (p &lt; 0.05). For carcass and meat quality, BSFLO supplementation did not affect dressing percentage or edible meat yield (p &gt; 0.05). However, it influenced meat pH and shear force (p &lt; 0.05), with a higher pH at 24 h post-mortem in BSFLO-fed chickens. Metabolomics showed that arginine biosynthesis; phenylalanine, tyrosine, and tryptophan metabolism; alanine, aspartate, and glutamate metabolism; arginine and proline metabolism; and taurine and hypotaurine metabolism were the most differentially abundant. These findings suggest that BSFLO can be used for a partial replacement (50 to 75%) for RBO in Thai native chicken diets, potentially offering benefits for animal health and meat quality without compromising growth performance.

Impacts of black soldier fly, Hermetia illucens, larval frass on the emergence and seedling vigor of three vegetable crop species

Abstract The waste material from rearing black soldier fly, Hermetia illucens (L.), (Diptera: Stratiomyidae), larvae (BSFL), frass, could serve as a partial replacement for fertilizer or growing media, such as peat. Studies have shown that depending on the crop life cycle, long or short, the incorporation of frass can have positive impacts on vegetable growth. Investigating the earlier stages of growth, such as emergence, can provide more information on the acute impacts of insect frass on plant production. Two greenhouse studies were designed to investigate the use of frass in vegetable production at the seedling stage: BSFL frass as a partial replacement for peat in a growth germination medium and BSFL frass as a fertility replacement in a growth germination medium. Tomato, lettuce, and arugula seeds were grown in a standard control growing media, a growing media amended with frass to partially replace peat, and a growing media with frass applications to substitute the fertility source. In all treatments and varieties, the control growing media produced better or comparable results to the treatments. As a peat replacement, the two tomato varieties, lettuce, and arugula seedlings grown in frass had comparable seedling vigour and days to emergence to those grown in the control. As a fertility replacement, seedlings grown in frass had significantly lower percent emergence in all four cultivars when compared to the control. The tomato varieties also had significantly lower seedling vigour in the BSFL fertility treatments than the control. Depending on crop and growing media composition, the impacts of frass on early plant development vary. The adoption of frass into growing practices should include preliminary tests for nutrient composition and microbial contamination.

Evaluation of black soldier fly larvae oil (Hermatia illucens L.) calcium salt as an alternative fat source for laying quail diets.

This study aims to determine the effect of adding saponified black soldier fly larvae oil calcium salt (BSFLO-SCa) to quail feed as an alternative source of fat on laying performance, blood lipid profile, egg quality, and gene expression in lipid metabolism. A total of 120 female Japanese quails (Coturnix japonica) aged 24 weeks were divided into 3 treatments, each with 8 replications, and each replication consisted of 5 quails in a completely randomized design. The applied treatments were the inclusion of basal feed as a control (T0) and basal feed supplemented with 1% BSFLO-SCa (T1) and 2% BSFLO-SCa (T2). The study indicated that the supplementation starting from 1% of BSFLO-SCa significantly decrease (p<0.05) in feed conversion ratio (FCR), blood lipid profile (total cholesterol, LDL-cholesterol). Gene expression on fat synthesis of fatty acid synthase (FAS) and cholesterol synthesis of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) downregulated (p<0.05). In addition, the other parameters did not affect by supplementation of 1% BSFLO-SCa. The inclusion at 2% of BSFLO-SCa significantly increased (p<0.05) protein content of yolk and albumen, egg weight, egg shape index, and gene expression on fat oxidation of carnitine palmitoyltransferase-1 (CPT-1). Egg yolk cholesterol, egg albumen ash, haugh unit (HU), and gene expression on fat synthesis of acetyl-CoA carboxylase (ACC) were significantly reduced (p<0.05). Addition of 2% BSFLO-SCa in the feed improves performance, egg quality, and reduces cholesterol in the blood and eggs of quail. This improvement is accompanied by a reduction in the expression of key genes involved in lipid metabolism. BSFLO-SCa oil has the potential to be an alternative oil source in quail feed.

Carbon footprints of centralized and decentralized food waste utilization pathways

Food waste is usually utilized in centralized treatment facilities and is sometimes treated in decentralized facilities; however, the latter has not been evaluated systematically regarding carbon emissions. This study surveyed fifty-nine centralized and decentralized food waste treatment projects in Shenzhen, and incineration, anaerobic digestion, composting, and emerging technologies such as insect bioconversion and organic acid production were applied. Their carbon footprints were analyzed based on real data covering collection and transport, energy and material consumption, secondary pollutant control, and impurity disposal. The results showed that centralized facilities had a lower overall carbon footprint, although carbon emissions from their collection and transportation were higher. Anaerobic digestion performed best with an average carbon emission of −136.63 kg CO2-eq/t, followed by black soldier fly larvae cultivation (−12.86 kg CO2-eq/t), incineration (−11.02 kg CO2-eq/t), and organic acid production (−6.86 kg CO2-eq/t). All decentralized facilities were operated with net carbon emissions because they consumed a large amount of electricity or thermal heat without the full use of organic matter in the food waste. An optimal route was recommended by combining centralized anaerobic digestion and decentralized squeezing dewatering with adequate oil recovery, and this can make the overall carbon emission decrease from −6.72 kg CO2-eq/t to −17.75 kg CO2-eq/t. These results can provide decision-makers with guidance on food waste management to support net zero and sustainable development goal 12 and 13.

Invertebrate composting quality of the invasive alga Rugulopteryx okamurae, prospects for its bio-recycling, management and circular economy.

In recent decades, the invasive seaweed Rugulopteryx okamurae has had a huge environmental impact on marine biodiversity, fisheries, GHG emissions and public health along much of the Iberian Peninsula and islands coastline. Due to the enormous amount of algae biomass that is expelled to the beaches where it slowly rots, some circular economy business initiatives, such as composting, are emerging. In the present study, we compared the quality of compost obtained from earthworms (Dendrobaena veneta), cockroaches (Eublaberus sp.), mealworms (Tenebrio molitor) and black soldier fly larvae (Hermetia illucens). Batches fed with 100% organic kitchen waste (control group) were compared with batches fed with 50% algae and 50% kitchen waste (treatment group). Our results show that the most sensitive species (D. veneta and T. molitor) to R. okamurae toxins compost adequately. The C/N ratio, electrical conductivity (EC), pH, total organic matter (TOM), C, K, K2O, Mg, MgO, N, P, P2O5, B, Cu, Ni and Zn of the compost obtained were determined. A high quality compost was obtained in which only the EC values are slightly elevated. Particularly good was the compost obtained with H. illucens and Eublaberus sp. This quality is in agreement with previous research on the mass balance of composting. Therefore, both species offer, in the field of circular economy, encouraging prospects for the development of composting enterprises.

The on growth, histology of intestinal and hepatopancreas of using black soldier fly (Hermetia illucens) larvae meal in the diets of Pontastacus leptodactylus juvenile

ABSTRACT In 60 days of feeding, a trial was conducted to evaluate the effects on liver, gut histology, and growth performance of using defatted black soldier fly Hermetia illucens (BSF) larvae meal as a fish meal (FM) protein alternative in the diet of Pontastacus leptodactylus. Fish meal protein was replaced with BSF meal protein at 0%, 20%, 40%, 60%, 80%, and 100%. Six hundred crayfish with an initial weight of 0.033 g were randomly divided into 24 experimental groups with four repetitions. There were no significant differences observed in cheliped, molting numbers, or survival rates between the groups (p > .05). However, adding more than 60% BSF to feeds caused pathological findings in the hepatopancreas,and the intestines. The results of this study showed that 40% defatted BSF meal can be used as a protein source replacement for fish meal protein without affecting the survival rate and growth of P. leptodactylus juveniles.

Turning trash into treasure: Hermetia illucens microbiome and biodegradation of industrial side streams.

Organic side streams from various industries pose a challenge to the environment. They are often present in huge amounts and are mostly discarded, incinerated, used for biogas production, or as feed for ruminant animals. Many plant-based side streams contain difficult-to-digest fiber as well as anti-nutritional or even insecticidal compounds that could harm the animals. These challenges can be addressed using black soldier fly larvae, which are known to degrade various organic substrates and convert them into valuable biomass. This will help mitigate agro-industrial side streams via efficient waste management and will contribute to the more economical and sustainable farming of insects.

Insect Production: A Circular Economy Strategy in Iceland

In this review, the multifaceted issue of food security is addressed, emphasizing the need for innovative and culturally appropriate solutions. Exploring insect livestock farming emerges as a potential remedy, offering a pathway to alleviate food insecurity and promote food sovereignty, particularly when integrated with social acceptability. Stakeholder engagement on both production and consumption fronts, coupled with sustained support, is vital for successful implementation. The expanding landscape of commercial insect farming in the West prompts questions about its broader scalability and equitable deployment, especially for vulnerable populations. Existing research gaps underscore the need for a coordinated effort across international, national, and legal frameworks to effectively integrate insect farming into existing agricultural systems. In this review, we have delved into the industrial-scale production processes of mealworms and black soldier flies (BSFs), known for their high protein content and organic waste conversion, covering small and industrial cultivation methods, offering insights into mealworm production life cycles, innovative rearing systems, and harvesting techniques. This review concludes with climate-specific recommendations for insect facilities, stressing the importance of sustainable practices, continuous research and development, effective market strategies and economic feasibilities in Iceland. In the context of escalating demand for sustainable protein sources, industrial-scale insect production emerges as a pivotal player in addressing global food security challenges.

The use of alternative protein sources in quail diets: A review

The growth of the Earth’s population and people’s striving for healthy life style lead to an increase in the global demand for protein food. Quails are a valuable source of protein, which is thought to reduce the risk of obesity and cardiovascular diseases. Inclusion of alternative protein sources into the composition of feeds for quails is an answer to ecological problems associated with the use of traditional protein sources. Plants, insects, algae and mushrooms are among alternative protein sources that are mentioned most frequently in scientific studies. The aim of this review is analysis of scientific literature dedicated to the use of plants, insects, algae and mushrooms as alternative protein sources in feedstuff for quails. Plant sources of protein, such as soya and maize, are successfully used in feedstuff production. However, studies have shown that addition of other sources, including by-products of crop husbandry, will allow increasing sustainability and reducing the dependence of the industry on traditional raw materials. Flour from black soldier fly (Hermetia illucens) larvae is the promising and most widely studied source of protein among insects and can positively affect productivity and health of quails. Among algae, it is necessary to mention spirulina (Spirulina platensis and Arthrospira platensis). Upon addition of 4% of the spirullina powder, productivity parameters in poultry increased. Mushrooms are described as a promising protein source in feedstuff that is also capable of ensuring an increase in productivity and quality of poultry meat. Today, however, there are few studies on this subject. It is recommended to focus further studies of the application of alternative protein sources in quail diets on questions of safety of their use and more detailed analysis of economic feasibility.

Comparison of Black Soldier Fly (BSF) Maggot Nutrition with Different Culture Media

Maggot Black Soldier Fly (Hermetia illucens) is one of the natural feeds widely used by fish farmers. Maggot has a high nutritional content so that it is in great demand by the community. This study aims to determine the nutritional value contained in maggot cultured using different culture media. The culture media used in this study include fermentation of tofu waste and fermentation of fish waste. Fermentation of culture media was carried out for 7 days using EM4, sugar and water. The parameters analyzed in this study included protein content, fat content, water content, ash content and carbohydrates. The results showed that maggots cultured using fish waste fermentation culture media had a higher protein content compared to tofu waste fermentation culture media. The nutritional value of the fish waste fermentation treatment was 33.94% protein, 8.13% moisture content, 2.85% ash content, 3.31% fat and 51.16% carbohydrate.

A First Step Towards Black Soldier Fly Larvae (Diptera: Stratiomyidae) Welfare by Considering Dietary Regimes (Part I).

The insect farming sector is expanding, but knowledge of insect welfare is still limited. This article aims to optimize the dietary regime for "black soldier fly" (Hermetia illucens L., BSF) larvae by applying a holistic view of welfare. Four diets were tested: control (CONTR, commercial laying hen feed), vegetable (VEG), omnivorous (OMN), and carnivorous (MEAT) diet, conducting experiments at a large (2000 larvae) and small scale (100 larvae). Rearing parameters were calculated including the growth rate, substrate reduction, efficiency of conversion of digested food, waste reduction index, and survival rate. Chemical analyses were conducted on BSF larvae and the residual frass. While the MEAT diet appears to be non-well-performing for the larvae, the VEG diet performed comparably to the control diet. Interestingly, the OMN diet demonstrated improved efficiency when evaluating the growth process at both scales. The chemical composition of larvae and frass highlighted the nutritional adequacy of the OMN diet, with the BSF larvae showing adequate protein and lipid content without nutrient catabolism or signs of discomfort. Applying the five freedoms of Brambell's report as a welfare standard for animal rearing and evaluating performance as an indirect indicator of welfare, the OMN diet appears to promote larval welfare in rearing practices.