Solanum Lyratum Thunb Research Articles

Explore on screening COX-2 inhibitors from the essential oil of Solanum lyratum Thunb. By molecular docking and molecular dynamics simulation

This study aimed to investigate Solanum lyratum Thunb. with respect to the potential ingredients with anti-inflammatory activity from its essential oil by silico study. To this regard, the essential oil of Solanum lyratum Thunb. was extracted by hydrodistillation. 25 compounds were identified by GC-MS. Using virtual screening, molecular docking and molecular dynamics simulation of the 25 identified compounds, the ones showing anti-inflammatory activity on COX-2 were identified. According to the drug-like principle and the prediction of ADEMT properties, the six compounds of Spathulenol, Cedrol, Juniper camphor, Santalol, Nootkatone and 7,9-Di-tert-butyl-1-oxaspiro[4.5]deca-6,9-diene-2,8-dione were identified and then studied for molecular docking, and based on which the top two compounds of binding free energy were studied by the molecular dynamics simulation. The molecular docking data indicated that the binding free energies of Spathulenol, Cedrol, Juniper camphor, Santalol, Nootkatone and 7,9-Di-tert-butyl-1-oxaspiro[4.5]deca-6,9-diene-2,8-dione to COX-2 protein were −5.65, −7.19, −6.35, −4.94, −5.82 and −5.14 kcal/mol, respectively. The findings showed the steady interactions of hydrogen bonds and hydrophobic bonds between both the top two compounds of binding free energy and the active site residues of COX-2 (4M11) throughout the simulation via hydrogen bonds and hydrophobic bonds. The very study shall be supportive for in vitro and in vivo studies in developing drug products using the lead bioactive ingredients for anti-inflammatory in the future.

Research progress on chemical components and pharmacological action of Solanum lyratum Thunb.

Solanum lyratum Thunb (SLT) is a perennial plant of the Solanaceae family, and is extensively used in the clinical practice of traditional Chinese medicine. Malaria, oedema, gonorrhoea, cancer, wind and fever, jaundiced hepatitis, cholecystitis and rheumatoid arthritis are among the diseases that it is used to treat. To offer a foundation for further development and usage of SLT, the pieces of literature about the chemical composition and pharmacological action of SLT were reviewed and analysed. The chemical constituents of SLT mainly included steroids, alkaloids, flavonoids, terpenoids, anthraquinones, phenylpropanoids and others. Pharmacological action mainly contains anti-tumour, antibacterial, anti-inflammatory, anti-oxidation and other pharmacological actions, among them, the anti-tumour effect is particularly outstanding. At present, studies on the pharmacological effects of SLT mainly focus on alkaloids and steroidal saponins. In the follow-up studies, studies on the pharmacological activities of other chemical components in SLT, such as flavonoids and terpenoids, should be strengthened. It has the potential to pave the way for more research and development of novel SLT medicines.

Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway

Ovarian cancer is a type of common gynecological tumors with high incidence and poor survival. The anticancer effects of the traditional Chinese medicine Solanum lyratum Thunb (SLT) have been intensively investigated in various cancers but in ovarian cancer is rare. The current study is aimed at investigating the effect of SLT on ovarian cancer cells. Lactate dehydrogenase (LDH) and MTT assays indicated that SLT concentrations of 0.25 and 0.5 μg/mL were not cytotoxic and had significant inhibitory effects on the cell viabilities of A2780 and SKOV3 cells, hence were used for subsequent experiments. Flow cytometric and western blot analysis revealed that SLT effectively suppressed ovarian cancer cell proliferation via inducing cell cycle arrest and increasing apoptosis. Cell cycle and apoptosis-related protein expressions were also regulated in SLT-treated cells. Moreover, DCFH-DA and western blot assays demonstrated that SLT enhanced ROS accumulation and subsequently activated the p53 signaling pathway. However, SLT-regulated ovarian cancer cell proliferation, apoptosis, migration, invasion, and EMT were significantly reversed by an ROS inhibitor (NAC, N-acetyl-L-cysteine). Furthermore, A2780 and SKOV3 cells cocultured with M0 macrophages showed that SLT activated the polarization of M0 macrophages to M1 macrophages and inhibited the polarization to M2 macrophages, with the increased percentage of CD86+ cells and decreased percentage of CD206+ cells were detected. In summary, this study illustrated the anticancer effects of SLT on ovarian cancer cells, suggesting that SLT may have the potential to provide basic evidence for the discovery of antiovarian cancer agents.

First report of tobacco vein banding mosaic virus infecting Solanum lyratum Thunb in China

First report of tobacco vein banding mosaic virus infecting Solanum lyratum Thunb in China

A study on the inhibitory effect of <i>Solanum lyratum thumb</i> extract on lewis lung carcinoma lines

The objective of this paper was to observe the effects of Solanum lyratum Thunb extract on tumour inhibition, immune function and survival time of tumour-bearing mice. Lung carcinoma-bearing mouse model was established, the tumour-bearing mice were divided into model group, CTX group, Solanum lyratum Thunb extract high-dose group and low-dose group. By the examination of tumour inhibition rate of Solanum lyratum Thunb extract in Lewis lung carcinoma-bearing mice and determination of the number of NK cells and T cell subsets, the survival rate of tumour-bearing mice was observed. Solanum lyratum Thunb extract had some anti-tumour effect in Lewis tumour-bearing mice. The tumour inhibition rate of high-dose group reached 46.28%, and the tumour inhibition rate of low-dose group was 31.42%. Solanum lyratum Thunb extract can improve the NK cell activity of Lewis tumour-bearing mice, increase the number of CD4 cells in the tumour-bearing mice, and significantly increase the survival rate of tumour-bearing mice. The study concluded that Solanum lyratum Thunb extract has some anti-tumour effect and can improve immune function and survival rate of tumour-bearing mice.

Study on anti-tumor effect of <i>Solanum Lyratum</i> thunb. Extract in S<sub>180</sub> tumor-bearing mice

The objective of the study was to investigate the anti-tumour effect of ethanol extract of Solanum lyratum Thunb. in S₁₈₀ tumour-bearing mice, and to preliminarily explore its mechanism of action. Mice were made into S₁₈₀ solid tumour model, grouped and administered. Tumour inhibition rate was measured by harvesting the tumours. Serum IL-2, TNF-a contents were measured by taking blood samples, and thymus index and spleen index were measured by harvesting the thymus and spleen. The results showed that the Solanum lyratum Thunb. extract had certain tumour inhibitory effect, which can elevate the serum IL-2, TNF-a contents, and increase the thymus and spleen indices to a certain extent. The study concluded that Solanum lyratum Thunb. extract has certain in vivo anti-tumour effect which may be exerted through enhancing the body immunity.

Induction of Cell Cycle Arrest and Apoptosis in Human Osteosarcoma U-2 OS Cells by Solanum lyratum Extracts

This research focused on a Chinese herb medicine, Solanum lyratum Thunb (Solanaceae) by ethanol extracts (SLE) for investigating the molecular anticancer mechanism in vitro for exploring the means of cell death through the effects on mitochondrial function. We found that SLE induced cytotoxic effects in human osteosacroma U-2 OS cells, and these effects include cell morphological changes, a decrease of the percentage of viable cells and induction of apoptosis. The results suggest that cell death induced by SLE is closely related to apoptosis based on the observations of DAPI staining and sub-G1 phase in U-2 OS cells. Flow cytometric assays also showed that SLE promoted the production of reactive oxygen species and nitric oxide but decreased the levels of mitochondrial membrane potential and promoted the activations of caspase-8 and -9 in U-2 OS cells. SLE inhibited the level of Bcl-2 but promoted the Bax level, and both proteins led to the release of cytochrome c from mitochondria to cytosol and activation of caspase-9 and -3, resulting in the apoptotic death which is mediated through the mitochondrial pathway. Taken together, SLE was demonstrated to be effective in killing U-2 OS osteosacroma cells via the ROS-promoted and mitochondria- and caspase-dependent apoptotic pathways.

Karyotype analysis of three Solanum plants using combined PI-DAPI staining and double fluorescence in situ hybridization with 45S and 5S rDNA probes

In this study, mitotic metaphase chromosomes of Solanum surattense Burm ., Solanum lyratum Thunb. and Solanum photeinocarpum Nakam. were well prepared using an advanced chromosome preparation method. The chromosomes were distinguished by combined PI-DAPI (CPD) staining and double fluorescence in situ hybridization (FISH) with 45S and 5S rDNA probes and their molecular cytogenetic karyotypes were established. Although, the karyotype of S. surattense Burm. and S. photeinocarpum Nakam was first established, the karyotype formulas of the three Solanum plants ( S. surattense Burm., S. lyratum Thunb. and S. photeinocarpum Nakam) can be described as follows: 2n=24=18m +4sm+2st (2SAT), 2n=24=18m +4sm+2st (2SAT) and 2n=24=18m +6sm (2SAT). Moreover, the karyotype asymmetry of the three species belongs to 2A type. After CPD staining, the centromeres of all chromosomes in the three species were shown as red CPD bands, indicating the presence of GC-rich DNA sequences in the chromosomes. Subsequential double FISH shows that S. surattense Burm., S. lyratum Thunb. and S. photeinocarpum Nakam, all have a pair of 45S rDNA sites located on chromosomes, and all of these 45S rDNA sites correspond to the respective prominent CPD banded regions. S. surattense Burm., S. lyratum Thunb. and S. photeinocarpum Nakam correspond to the CPD banded regions at the ends of chromosomes 8, 10 and 12, respectively. The three Solanum plants all have a pair of 5S rDNA sites. In S. surattense Burm., the 5S rDNA sites are located on the long arm of the 6th chromosome pair, while the 5S rDNA sites of S. lyratum Thunb. and S. photeinocarpum Nakam are all located on the short arms of the 4th chromosome pair. Our study shows that the CPD bands and rDNA FISH signals provide effective chromosome markers allowing us to establish accurate molecular cytogenetic karyotypes of the three species tested. Key words: Solanum surattense Burm. Solanum lyratum Thunb., Solanum photeinocarpum Nakam , karyotype, CPD staining, fluorescence in situ hybridization (FISH).

Nitrogen-containing compounds from Solanum lyratum Thunb

Abstract The medicinal plant Solanum lyratum Thunb（Solanaceae）is distributed widely in China. The present phytochemical investigation on S. lyratum led to the isolation of ten known Nitrogen-containing compounds. Compounds 1, 2, 3, 6, 9 were isolated for the first time from this plant and compounds 8,10 were obtained for the first time from Solanum . Compound 10 was isolated from Solanaceae family and obtained outside from Loganiaceae family for the first time. This has important chemotaxonomic significance on solanaceae.

Immunomodulatory activity of butanol extract from Solanum lyratum in tumor-bearing mice

Solanum lyratum Thunb (Solanaceae) has been widely used for cancer as a folk remedy in Chinese traditional medicine. In this study, the main active fraction n-butanol extract from S. lyratum (BESL) was evaluated for the therapeutic efficacies on mice transplantable tumor and immunomodulatory potentials on the immune response in tumor-bearing mice. The effects of BESL on the growth of mouse transplantable S180 sarcoma, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL), production of cytokines from splenocytes, and serum antigen-specific antibody levels in S180-bearing mice were measured. BESL could not only significantly inhibit the growth of S180 sarcoma transplanted in mice, but also remarkably promote splenocytes proliferation, NK cell and CTL activity, interleukin-2 and interferon-γ production from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice (P < 0.05, P < 0.01, or P <0.001). The results suggested that BESL might exhibit antitumor activity by improving immune response, and it could act as antitumor agent with immunomodulatory activity. This study provided evidence to understand the therapeutic effects of S. lyratum for treatment of cancer and a natural product to further researches to be developed as a cancer chemopreventive agent.

Isolation, purification and structural investigation of a water-soluble polysaccharide from Solanum lyratum Thunb

Polysaccharides, extracted from the herbs of Chinese natural Solanum lyratum Thunb (SLT), which is a traditional Chinese medicine with hot water and 0.04 M sodium hydroxide successively, were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation, periodate oxidation, NMR spectroscopy, partial and graded acid and enzymic hydrolysis analysis, the results indicated the d-glucan to be linear and to contain both (1 → 3)- and (1 → 4)-linkages. The anomeric NMR and IR spectra measurements confirmed that the sugar residues were β-glycosidically linked.

Abolition of anaphylactic shock by solanum lyratum Thunb

We investigated the effects of the aqueous extract of Solanum lyratum T hunb. (Solanaceae) (SLAE) on the anaphylactic reactions. SLAE inhibited compound 48/80-induced anaphylactic shock 100% with a dose of 1.0 mg/g body weight (BW) . When SLAE was pretreated at concentration ranging from 0.0001–1.0 mg/g BW, the serum histamine levels were reduced in a dose-dependent manner. Passive cutaneous anaphylaxis also inhibited to 69.3% by oral administration of SLAE (0.05 mg/g BW) . Moreover, SLAE dose-dependently inhibited the histamine release from the rat peritoneal mast cells (RPMC) by compound 48/80. The level of cAMP in RPMC, when SLAE was added, significantly increased compared with that of normal control. These results indicate that SLAE possess strong antianaphylactic activity.

Studies on the constituents of solanaceous plants. XIII. A new steroidal glucuronide from chinese Solanum lyratum.

A new glucuronide, (22R)-3β, 16β, 22, 26-tetrahydroxycholest-5-ene 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-gluc-uronopyranoside (1), was isolated from the aerial parts of Chinese Solanum lyratum THUNB. The structure of this new steroidal glucuronide was deduced by spectroscopic means and the identity of its aglycone was substantiated by comparison with an authentic sample derived from diosgenin acetate.