Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea(Received on October 30, 2013; Revised on November 12, 2013; Accepted on November 19, 2013)Totally sixty three bacteria were isolated from lower stems showing symptoms of bacterial wilt on pepperplants in 14 counties of 7 provinces, Korea. The isolates showed strong pathogenicity on red pepper (cv.Daewang) and tomato (cv. Seogwang) seedlings. All virulent bacteria were identified as Ralstoniasolanacearum based on colony types, physiological and biochemical tests and polymerase chain reaction(PCR). All R. solanacearum isolates from peppers were race 1. The bacterial isolates consisted of biovar 3(27%) and biovar 4 (73%). Based on polymorphic PCR bands generated by repetitive sequence (rep-PCR),the 63 R. solanacearum isolates were divided into 12 groups at 70% similarity level. These results will be usedas basic materials for resistant breeding program and efficient control against bacterial wilt disease of pepper.Keywords : Biovar, Classification, Pepper, Ralstonia solanacearum, Rep-PCR