Soils In Poland Research Articles

An investigation of rare earth elements in sewage sludge generated in Poland

According to Statistics Poland, an average of around 1 million tonnes DM of sewage sludge has been generated in Poland annually over the past several years, of which approximately 30% has been used in nature, e.g. in agriculture, to grow plants for compost production, or for land reclamation (Statistics Poland 2004–2022). Most research on sewage sludge has focused on investigating its fertilizing value (nitrogen, phosphorus), identifying the composition of organic matter and determining the total content of heavy metals (including primarily cadmium, copper, nickel, lead, zinc, mercury and chromium) and the forms of their occurrence that determine their mobility and bioavailability. The occurrence of rare earth elements (REEs) in sewage sludge has hardly been addressed in research, even though their presence in production processes and everyday objects is increasingly common. The results presented in this article of studies of the concentrations of individual REEs in sewage sludge from selected industrial and municipal wastewater treatment plants located in Poland indicate that they are significantly lower than the average lanthanide level in the Earth’s crust. This may suggest that anthropogenic sources of REEs do not affect the composition of the wastewater and sludge studied. The calculated median concentration of ΣREE in sludge from industrial wastewater treatment plants is 9.47 mg/kg, whereas in municipal sewage sludge, the midpoint value for REE concentration is 13.5 mg/kg. Normalization of the obtained results with respect to Post-Archean Australian Shale (PAAS) and to topsoil and subsoil from Poland shows that the sludge is generally depleted in REE relative to the standards used. An assessment of the contamination of sewage sludge with rare earth elements, based on the calculated values of the geoaccumulation index (Igeo) for these elements, also shows that the content of lanthanides in the studied sewage sludge is lower than in the soils of Poland.

Assessment of soil CO2, CH4, and N2O fluxes and their drivers, and their contribution to the climate change mitigation potential of forest soils in the Lublin region of Poland

AbstractForests can play a key role in the mitigation of climate change, although there have been limited regional scale assessments that account for variations in soil type and tree species. Most of the focus has been on their ability to sequester atmospheric CO2, while there is less information on the two other major greenhouse gases (GHGs), N2O and CH4. We examined the GHG budgets of ten forest soils in Poland, considering all three major GHGs, where no previous long-term measurements had been made, which encompassed different tree species, stand age, and contrasting edaphic conditions. In addition to the quantification and assessment of seasonal variability in the major soil GHG fluxes over two years, the aims of the present study were (i) the identification of the main drivers of the soil-based GHG fluxes, (ii) the determination of the contribution of each gas to the Global Warming Potential (GWP), and (iii) to assess the mitigation potential of these fluxes over different forest systems. All the forest soils were sources of CO2 and N2O and sinks for atmospheric CH4 with pronounced seasonal variations in CO2 and CH4 driven by soil moisture and temperature. The soils showed significant differences in annual GHG fluxes, with average values of 16.7 Mg CO2 ha−1, − 3.51 kg CH4 ha−1, and 0.95 kg N2O ha−1. The annual total GWP ranged from 13.1 to 22.0 Mg CO2 eq ha−1 with CO2 making the highest contribution, and forest-specific CH4 uptake resulting in a reduction in GWP, ranging from − 0.08% (in the youngest forest) to -0.97% (in the oldest forest). Mixed forests showed the greatest potential for climate change mitigation, with the highest soil C sequestration, and the lowest GWP values when compared to sites with monocultures. The results suggest that a mixture of tree species could eventually be incorporated into management plans to increase the effectiveness of forests in climate change mitigation.

Origin, transformation and classification of organic soils in Poland

Origin, transformation and classification of organic soils in Poland

Fertility and quality of arable soils in Poland: spatial–temporal analysis of long-term monitoring

Soil quality is a globally important issue from the point of view of food production, maintenance of appropriate environmental quality and ecological balance. Assessing its quality and fertility using indices is one of the methods that make it possible to formulate recommendations for good soil management. The aim of the study was, therefore, to determine the spatial and temporal distribution of soil properties in the context of soil quality assessment (SQI) and soil fertility assessment (SFI, SEF, NIV, and SSFI) using the example of a Central European country (Poland). The inverse distance-weighted spatial interpolation technique was used to estimate the spatial distribution of soil quality and fertility indicators. So far, no research has been carried out that would include a comprehensive analysis of soil fertility and quality indicators in such a large research area over many years. The results of 216 points of monitoring the chemistry of arable soils in Poland from the period 1995–2020 for 28 physicochemical soil parameters were considered. The analyses carried out showed significant variability in the properties of the described soils in Poland, as evidenced by the principal component analysis (PCA) and the coefficient of variation (exceptions: bulk density, pH). It was found that heavy metals (Zn, Cd, Cu) were responsible for the deterioration of soil properties. The results obtained for different indices were different (SFI – most soils with good fertility, SEF – high fertility, NIV – soils highly enriched with nutrients, SQI – average soil quality). It was found that the SSFI is the most appropriate indicator among the analyzed indicators for Polish conditions in relation to arable soils, as it is adapted and calibrated to the specifics of this area. The Spearman correlation matrix showed that the largest group of statistically highly significant parameter pairs belonged to heavy metals (Ni/Zn, Zn/Pb, Zn/Cu, Ni/Cu). In contrast, the strongest correlations were recorded for Ni (with effective cation exchange capacity (ECEC), Mn, Mgav, Zn, Cu). The assessment revealed limiting factors and temporal trends in the overall evaluation of the quality of the country’s agricultural soils, which are important from the point of view of both sustainable development and agricultural production.

Assessment of heavy metal contamination of agricultural soils in Poland using contamination indicators

Heavy metals are considered a major pollutant due to their resistance to biodegradation, toxic effects on organisms, the fact that they can persist for long periods of time in soils and translocation. So far, no comprehensive, retrospective analyses have been carried out in Poland to assess spatial and temporal changes in contamination of agricultural soils with heavy metals (Cr, Cd, Cu, Ni, Pb, Zn, Co, V) using toxicity indices. The main objectives of the present study were to comprehensively assess the status of contamination of agricultural soils in Poland with selected heavy metals based on several indicators: Geoaccumulation Index (Igeo), Contamination Factor (CF), Potential Ecological Risk Index (ER), Enrichment Factor (EF), Pollution Index (PI) and Nemerow Pollution Index (NPI). Multivariate statistical analysis was also carried out, including principal component analysis (PCA) and descriptive statistics presented in box plots. Analyses were conducted over a period of 25 years at 216 study points within the framework of the chemistry monitoring of Poland's arable soils. This study will provide considerable assistance in the different soil types of Poland on the basis of toxicity indices. The presented research results show the spatial and structural diversification of soil contamination with metals and can be used in practice to formulate recommendations for rational soil use and planning investment activities in the field of environmental protection. In addition, these studies may be useful in the development of planning documents, especially in the development of postindustrial areas. At the analysed points, the potential toxicity of soils in relation to heavy metals was influenced by industrial activities in southern Poland (Upper Silesia) and south-western Poland (Lower Silesia); however, these exceedances affected few points (usually 2–3 of the 216 assessed). This situation persisted at these sites throughout the study period. The dominant type of contaminated soils was cambisols and phaeozems.

The Use of CPTU and DMT Methods to Determine Soil Deformation Moduli—Perspectives and Limitations

Abstract The article presents the concept of determining constrained modulus—M0 , initial shear modulus—G0 , Young modulus—E, and rigidity index—IR on the basis of parameters from static penetration tests CPTU (Piezocone Penetration Testing), SCPTU (Seismic Piezocone Penetration Testing) and dilatometer tests DMT (Flat Dilatometer Test), SDMT (Seismic Flat Dilatometer Test). The basis for constructing the empirical relationships between the mentioned modules and parameters from the CPTU and DMT studies was to determine the factors that affect these relationships. The article discusses the impact of the following factors; geological and geotechnical conditions, conditions of recording measurements in CPTU and DMT tests, factors relating to the CPTU and DMT testing methods, factors affecting reference parameters from laboratory tests, factors related to subsoil properties. The basis for obtaining the empirical relationships for determining the analyzed modules and rigidity index were extensive research of the soils of various origins, in Poland. Measurement uncertainties and factors influencing the recorded parameters in the CPTU study were documented by the studies of the Norwegian Geotechnical Institute and the former Department of Geotechnics of the Agricultural University in Poznań. In these studies, penetrometers from several reputable manufacturers were used. The article summarizes the established empirical relationships for individual modules, taking into account the effect of overconsolidation. It also comments on the interrelationship between constrained modulus M0 from CPTU and DMT test for soils in Poland.

Microplastic Pollution in EU Farmland Soils: Preliminary Findings from Agricultural Soils (Southwestern Poland)

Agricultural soils are considered as “hot-spots” of plastic particles; however, due to a lack of standardized method of microplastic determination in soils, as well as no legal regulations requiring the monitoring of the soil environment in the context of microplastic contamination, the data on MP abundance and occurrence in European soils are very limited. In this first study of MPs pollution in agricultural soils in Poland, we developed a method of microplastic extraction from soil samples with different properties (particle size distribution, clay and organic matter content) and used optical microscopy for MP determination and quantification. In this study, we analyzed 44 soil samples from five sampling site locations with differing soil type, agricultural activity, including farmland soils on floodplains and past records of sewage sludge and compost applications. We found evidence that 93% of cultivated soils in the SW part of Poland contained MPs. The content of MP varied between soil types and present/former use of the land. Loamy and clay soils contained more MPs, 1540 ± 912 particles per kg soil and 933 ± 682 particles per kg, respectively, compared with sandy soils at 383 ± 188 particles per kg of soil. The highest MP concentrations were determined in soils amended with sewage sludge, wastewaters and green-waste composts (up to 4050 ± 2831 particles per kg of soil). The wide distribution of MPs with a dominance of plastic fibers (up to 60% of determined MP types) can be associated with agricultural sources such as soil mulching, the use of organic fertilizers, seed coating or unintentional waste dumping and air deposition.

Copper and cadmium content in Polish soil: Analysis of 25‐year monitoring study

AbstractMetals can accumulate in agricultural soils, presenting a serious threat to human health; therefore, it is important to analyze the quality of these soils to avoid possible harm related to their contamination in the future. Despite the importance of monitoring arable soil quality, few studies have examined the current state of Cu and Cd soil contamination through analysis of historical data and temporal trends in heavy metal content. Therefore, this study was aimed at analyzing the spatial variability of Cu and Cd content (expressed by toxicity indices) and assessing the level of contamination of arable soils in Poland over the last 25 years (1995–2020). The average Cu content in soil throughout the duration of the study was ~10 mg/kg. The average Cd content increased from 0.7 mg/kg (in 1995) to 3.4 mg/kg (in 2020). The evaluation of Cu and Cd soil contamination showed that soils contaminated with Cd and Cu constituted 1.4% and 2.3% of the total monitoring points, respectively. The geoaccumulation index and pollution index ranged for Cu from −5.23 to 3.09 (mean: −2.50) and from 0.02 to 6.40 (mean: 0.20), and for Cd from −5.23 to 6.92 (mean: −1.67) and from 0.01 to 60.58 (mean: 0.44). The soil was practically uncontaminated with Cu and Cd in 98.77%/98.92% and 93.44%/97.92% of cases, respectively. On a national scale, the contents of Cu and Cd in soils depend on soil properties (pH, C, OM, ST, and CM) to a very small extent. An assessment of the spatial distribution of Cu and Cd concentrations in Polish arable soils indicated regional differences related to the degree of industrialization/urbanization. The obtained results show the impact of human activity on the level of heavy metals present in soils.

Soil-borne Ophiostomatales species (Sordariomycetes, Ascomycota) in beech, oak, pine, and spruce stands in Poland with descriptions of Sporothrixroztoczensis sp. nov., S.silvicola sp. nov., and S.tumida sp. nov.

Ophiostomatales (Ascomycota) contains many species, most of which are associated with bark beetles. Some members of this order are plant or animal pathogens, while others colonize soil, different plant tissues, or even carpophores of some Basidiomycota. However, little is known about soil-inhabiting Ophiostomatales fungi. A survey of these fungi associated with soil under beech, oak, pine, and spruce stands in Poland yielded 623 isolates, representing 10 species: Heinzbutiniagrandicarpa, Leptographiumprocerum, L.radiaticola, Ophiostomapiliferum, O.quercus, Sporothrixbrunneoviolacea, S.dentifunda, S.eucastaneae, and two newly described taxa, namely Sporothrixroztoczensissp. nov. and S.silvicolasp. nov. In addition, isolates collected from fallen shoots of Pinussylvestris that were pruned by Tomicus sp. are described as Sporothrixtumidasp. nov. The new taxa were morphologically characterized and phylogenetically analyzed based on multi-loci sequence data (ITS, β-tubulin, calmodulin, and translation elongation factor 1-α genes). The Ophiostomatales species were especially abundant in soil under pine and oak stands. Leptographiumprocerum, S.silvicola, and S.roztoczensis were the most frequently isolated species from soil under pine stands, while S.brunneoviolacea was the most abundant in soil under oak stands. The results highlight that forest soil in Poland has a wide diversity of Ophiostomatales taxa, but further studies are required to uncover the molecular diversity and phylogenetic relationships of these fungi, as well as their roles in soil fungal communities.

Mercury and methylmercury in Baltic Sea sediments, and Polish and Lithuanian soils

We review the current environmental pollution by mercury in the soils of Poland and Lithuania and in the sediments of the Baltic Sea. Mercury is documented to have many negative impacts on the environment as a toxic trace element. In many different chemical forms, it is being released into the environment by both geogenic and anthropogenic activities, with most being released from anthropogenic sources. Methylmercury is considered one of the most toxic forms found in the environment. Mercury levels in sediment and various point sources increased after World War II in the Baltic Sea, which was used as a dumpsite. Previous studies show noticeable differences in total mercury in the Baltic Sea. In the Warta and Odra rivers in Poland, mercury levels are also higher than the background value, though recent findings suggest that river sediments are not the main source of mercury to marine sediments. Concentrations in soils in Poland and Lithuania were below the level of limit values (1 and 1.5 mg/ kg −1 respectively), but Upper Silesia showed concentrations (up to 4.01 mg·kg −1 ) above the limit values. Furthermore, between 1992 and 2006, mercury levels in Wrocław dropped dramatically. The dominant trees in the area can affect mercury accumulation. No data were available for comparison with the soils in Estonia and Latvia.

Efficiency of early spring feeding of winter wheat complex fertilizers on light podzolic soil in Poland

Field experiments were carried out on sandy loamy podzolic soil in the Łdź Voivodship (Wieluń commune, Masłowice parish) at the Experimental Station of the Polish Research Variety Testing Center, to study the effect of various winter wheat feeding systems with an intensive cultivation system on yield and grain quality indicators. The soil before conducting the experiments was characterized by a neutral reaction of the soil environment, a very high level of mobile phosphorus, and an average content of mobile potassium in 2017 and 2019, and high availability in 2018. The initial level of nitrate nitrogen in the soil was medium in 2017 and high in 2018 and 2019. The podzolic soils of the region, with a light granulometric composition, often have a low supply of sulfur available to plants. With a low content of humus in such soils, there is a small reserve of sulfur in organic form, and therefore, variants with sulfur-containing fertilizers were included in the intensive technology of winter wheat cultivation. Weather conditions of the growing seasons 2017-2018, 2018-2019 and 2019-2020 differed by an insufficient amount of atmospheric precipitation during the growth stages critical for grain formation. The yield of winter wheat grain in all years of research was maximum in variant 3 with the use of ammonium nitrate and complex sulfur-containing fertilizer Apaviva NPK(S) 15:15:15(10) as top dressing. The yield increase resulted from the autumn phosphorus-potassium application (double superphosphate and potassium chloride) was 25, 34 and 30 % in 2018, 2019 and 2020, respectively. The maximum grain yield of winter wheat in the experiment of 6.93 t/ha was obtained in variant 3 in 2019. Such a system of winter wheat top dressing provided a stable high additional income.

Novel Antibacterial, Cytotoxic and Catalytic Activities of Silver Nanoparticles Synthesized from Acidophilic Actinobacterial SL19 with Evidence for Protein as Coating Biomolecule.

Silver nanoparticles (AgNPs) have potential applications in medicine, photocatalysis, agriculture, and cosmetic fields due to their unique physicochemical properties and strong antimicrobial activity. Here, AgNPs were synthesized using actinobacterial SL19 strain, isolated from acidic forest soil in Poland, and confirmed by UV-vis and FTIR spectroscopy, TEM, and zeta potential analysis. The AgNPs were polydispersed, stable, spherical, and small, with an average size of 23 nm. The FTIR study revealed the presence of bonds characteristic of proteins that cover nanoparticles. These proteins were then studied by using liquid chromatography with tandem mass spectrometry (LC-MS/ MS) and identified with the highest similarity to hypothetical protein and porin with molecular masses equal to 41 and 38 kDa, respectively. Our AgNPs exhibited remarkable antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. The combined, synergistic action of these synthesized AgNPs with commercial antibiotics (ampicillin, kanamycin, streptomycin, and tetracycline) enabled dose reductions in both components and increased their antimicrobial efficacy, especially in the case of streptomycin and tetracycline. Furthermore, the in vitro activity of the AgNPs on human cancer cell lines (MCF-7, A375, A549, and HepG2) showed cancer-specific sensitivity, while the genotoxic activity was evaluated by Ames assay, which revealed a lack of mutagenicity on the part of nanoparticles in Salmonella Typhimurium TA98 strain. We also studied the impact of the AgNPs on the catalytic and photocatalytic degradation of methyl orange (MO). The decomposition of MO was observed by a decrease in intensity of absorbance within time. The results of our study proved the easy, fast, and efficient synthesis of AgNPs using acidophilic actinomycete SL19 strain and demonstrated the remarkable potential of these AgNPs as anticancer and antibacterial agents. However, the properties and activity of such particles can vary by biosynthesized batch.

Genome-based classification of Streptomyces pinistramenti sp. nov., a novel actinomycete isolated from a pine forest soil in Poland with a focus on its biotechnological and ecological properties.

A genomic-based polyphasic study was undertaken to establish the taxonomic status and biotechnological and ecological potential of a Streptomyces strain, isolate SF28T, that was recovered from the litter layer in a Polish Pinus sylvestris forest. The isolate had morphological characteristics and chemotaxonomic properties consistent with its classification in the genus Streptomyces. It formed long straight chains of spores with smooth surfaces, contained LL-diaminopimelic acid, glucose and ribose in whole-organism hydrolysates, produced major proportions of straight, iso- and anteiso- fatty acids, hexa- and octa-hydrogenated menaquinones with nine isoprene units and had a polar lipid pattern composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, glycophospholipids and three uncharacterized components. Phylogenetic trees prepared using 16S rRNA gene and multilocus gene sequences of conserved housekeeping genes showed that the isolate formed a branch that was loosely associated with the type strains of several validly published Streptomyces species. A draft genome generated for the isolate was rich in natural product-biosynthetic gene clusters with the potential to produce new specialised metabolites, notably antibiotics, and stress related genes which provide an insight into how it may have become adapted to the harsh conditions that prevail in acidic forest soils. A phylogenomic tree based on the genomes of the isolate and its phylogenetic neighbours confirmed that it formed a distinct lineage well separated from its closest evolutionary relatives. The isolate shared low average nucleotide identity and digital DNA:DNA hybridization values with its phylogenomic neighbours and was also distinguished from them using a combination of cultural and micromorphological properties. Given this wealth of taxonomic data it is proposed that isolate SF28T (= DSM 113360T = PCM 3163T) be classified in the genus Streptomyces as Streptomyces pinistramenti sp. nov. The isolate showed pronounced antimicrobial activity, especially against fungal plant pathogens.

Heat Capacity of Drained Peat Soils

Soil-specific heat capacity (cp) and volumetric heat capacity (Cv) are recognized as a fundamental soil property essential for the accurate prediction of soil temperature and heat flow. This study presents the analysis of these thermal properties for drained peat soils in Poland. The objectives of this study were to (i) measure and develop a method for determining cp, (ii) analyze the (Cv) data for undisturbed soil samples from surface layers, and (iii) test the applicability of the cp value for calculating Cv of drained peat soils using the mixing model concept. The cp value was measured under laboratory conditions using a modulated differential scanning calorimetry (MDSC) for 18 soil layers sampled in six degraded peat soil profiles. The Cv was estimated for undisturbed triplicate soil samples from the 22 depths (66 samples) by using a dual-needle probe. The cp data for the organic soils were linearly temperature-dependent (MDSC) for the temperature range considered (−20–30 °C). The overall average cp value was equal to 1.202 J g−1 K−1 at a temperature of 0 °C. An increment in temperature of 1 °C corresponded to an increase in cp of 0.0043 J g−1 K−1 on average. Nevertheless, the lowest cp value was obtained for moss samples whereas the highest value represents alder peats. The Cv data measured using the heat thermal probe (HTP) method changed linearly with changes in the soil moisture content (θv) of the moorsh soils. The volumetric heat capacity calculated using the mixing model was comparable to the mean of measured values obtained on the triplicate samples.

Effects of Using Calcium Mine Drainage Wastewater for Irrigation on Changes in Chemical Composition of Selected Forest Soils in Poland

Effects of Using Calcium Mine Drainage Wastewater for Irrigation on Changes in Chemical Composition of Selected Forest Soils in Poland

Circulation of Selenium in the Environment

Abstract In order to function properly, every living organism must have favourable conditions for its operation, i.e. it must be systematically supplied with the necessary nutrients. On the example of selenium (Se), it can be seen how important is the right balance between providing the right amount of it, without exceeding the level above which it is toxic. The amount of Se in soil is closely correlated with its content in the parent rock; therefore, it differs depending on the soil type and may change even in a small area. Considerable dispersion of the element in the soils of Poland is related to their formation, mainly from dump materials of various glaciations. The problem of maintaining the balance between deficiency and excess of Se in the diet of humans and animals is related primarily to the uneven distribution of this element in nature. In this paper, on the basis of scientific literature, the current issues related to the deficiency and excess of Se in the soil and its possible sources are presented. The relationship between the content in the soil and the supply of Se in plants, animals and humans was also shown. The aim of this study was to summarise the state of knowledge on the complexity of Se occurrence in the environment and its importance in the soil-plant-animals-human system.

Catenulispora pinistramenti sp. nov., novel actinobacteria isolated from pine forest soil in Poland.

The taxonomic status of two filamentous actinobacteria, isolates NF23 and NL8T, recovered from the litter layer of a pine forest soil in Poland was established in a genome-based polyphasic study. The isolates showed a combination of chemotaxonomic, morphological and physiological properties associated with their classification in the genus Catenulispora. They formed a well supported lineage within the Catenulispora 16S rRNA gene tree and were most closely related to the type strains of Catenulispora acidiphila (99.1%), Catenulispora pinisilvae (99.9 %) and Catenulispora rubra (99.1 %), and like them, were found to have large genomes (10.8 and 11.5 Mbp, respectively). A phylogenomic tree based on the draft genomes of isolates NF23 and NL8T and their phylogenetic neighbours showed that they formed a distinct branch in the Catenulispora clade that was most closely related to C. pinisilvae DSM 111109T. The isolates shared a combination of genomic, genotypic and phenotypic features, and had high average nucleotide index (ANI) and digital DNA:DNA hybridization (dDDH) similarities consistent with their assignment to the same species. The isolates were distinguished from the C. acidiphila, C. pinisilvae and C. rubra strains by a wealth of taxonomic data and by low ANI (84.9-93.9 %) and dDDH (29.6-54.7 %) values. It is proposed that the isolates be classified in the genus Catenulispora as C. pinistramenti sp. nov. with isolate NL8T (=DSM 111110T=PCM 3045T) as the type strain. The genomes of strains NF23 and NL8T are rich in natural product-biosynthetic gene clusters hence these strains have the potential to synthesize new specialised metabolites.

Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product.

Simple SummaryEntomopathogenic nematodes (Steinernematidae) are obligate insect parasites and are used for insect pest control, particularly on amenity grassland and in horticulture. Extensive surveys have been conducted across the globe to isolate locally adapted entomopathogenic nematodes species. The biological activity and morphology of three new isolates of Steinernema feltiae from Poland are described. New S. feltiae isolates from Poland showed close genetic similarity to other isolates of this species and exhibited a high reproductive rate and invasive capacity compared to the commercial biopesticide.Insect trap studies were carried out to determine the presence of entomopathogenic nematodes (EPN) from the family Steinernematidae in the soils of Poland and to compare the biological activities of field nematode isolates with nematodes from commercial biopesticide. The fauna of these organisms in central Poland is poorly studied in both taxonomic and biological terms. Tilled soils representative of this region were sampled from cultivated fields. EPN were isolated from soil samples under laboratory conditions and identified using a key for species identification and molecular analysis. Basic morphometric parameters of infective juveniles and adult males of the first generation were determined. The research showed that males and infective juveniles Steinernema feltiae from Łoniów were the largest. The smallest infective juveniles were found in the isolate from Oblasy, and the smallest males in the isolate from Danków. In Poland, new field isolates showed close genetic similarity to other S. feltiae isolates. The research showed that the field isolates from Poland had greater infectivity and rate of reproduction compared with nematodes from the commercial biopesticide. The findings indicate the potential use of field S. feltiae isolates from Poland (iso1Lon, iso1Dan and iso1Obl) to develop new biopesticide products.

Occurrence of Azotobacter spp. in cultivated soils in Poland

Azotobacter spp. are soil bacteria capable of fixing atmospheric nitrogen and making it available to plants. This makes them microorganisms of hight importance to the agriculture. The distribution of these bacteria is a complicated subject and is correlated with diverse factors, witch determine the presence or absence of the bacteria in a specific soil. The soil properties as soil and fertility and climate conditions affect the abundance of diazotrophs. Research material consisted of 182 soil samples taken in 2015 from agricultural land in various voivodeships. The number of Azotobacter spp. was determined based on plate method. The presented study evaluated the abundance of Azotobacter spp. in various Polish soils and confirmed the relationship between soil properties and the presence of these bacteria. The studied bacteria were present in 37% of soil samples and numbers of these bacteria varied widely, from 3 to 10801 cfu g-1 of the soil. In the case of Azotobacter spp., soil acidity was also an important factor restricting the occurrence of these bacteria in soils.

Anthropogenic records in a fluvial depositional system: The Odra River along The Czech-Polish border

Industrial revolution induced contamination of lowland rivers and floodplain soils with heavy metals worldwide. This pollution was particularly significant in former coal mining regions where hard coal was utilised in various industrial facilities like coke, iron and steel works. The upper Odra River draining Ostrava Coal District in Czech and Upper Silesia mining region in Poland is an example of such a European river with strong historical pollution followed by modern one originating from heavy industry in times of communistic industrialisation in both countries. In the presented studies we sought 19th and 20th century transboundary contamination of the upper Odra River waters recorded in floodplain soil by the most common potentially toxic elements, i.e. lead and zinc as well as by the presence of magnetic particles (spherules). We expected to reveal the general pattern of the Odra River floodplain soil contamination with risk elements which allow us to differentiate sediments of the industrial and pre-industrial era. We used robust regression and robust principal component analysis, using a log-ratio methodology of compositional data analysis, which is a fast and effective tool in assessing contamination levels. Significant increases in risk element concentrations were found downstream from the Ostrava–Bohumín agglomeration, and in some places they exceed the safety limits for agricultural soils in Poland. A comparison of sediments within and outside of the inter-embankment zone showed no systematic difference in their contamination levels suggesting that the sediments were probably contaminated before dike construction mainly since the half of 19th century.