One of the major universal threats is water pollution. The untreated distilleries effluent discharge into the environment creates a serious problem to the living systems. It may contain carcinogenic aromatics amines, dyes, organic and inorganic chemical compounds. The physicochemical characterization of effluent was collected from Trichy Distilleries & Chemicals pvt Ltd, Tiruchirappalli, Tamilnadu, India and the chemical analyzed in Soil Testing Laboratory, Tiruchirappalli. The effluent was analyzed by different parameters. The physical parameters are BOD, COD, and DO, TDS, pH and electrical conductivity. Heavy metal analysis included Zinc, Lead, Manganese, Arsenic, Iron, Mercury, Copper, Chromium, Cadmium and Nickel. The soil sample was taken from Trichy Distilleries and Chemicals Ltd and analyzed by serial dilution technique. The three microbes such as Escherichia coli, Bacillus subtilis and Pseudomonas putida, were isolated from soil source. The isolated organism was treated with the effluent for various concentrations in various hours. The metal accumulations were identified in soil test lab, Trichy. The hyper accumulations of metal in microbes were identified. The present study explores the effectiveness of bioremediation has a simultaneous removal of Zinc, Lead, Manganese, Arsenic, Iron, Mercury, Copper, Chromium, Cadmium and Nickel from the distilleries effluent. Bacillus subtilis bacterial strains effectively remove the heavy metal from the distilleries effluent.