Abstract Six accessions of edible amaranths (Amaranthus spp. L.) of varied geographic and genotypic origin were grown in a soil enriched with 0, 50, or 100 kg·ha–1N. Leaves were harvested at 25, 35, 45, 55, and 65 days after germination (DAG) and analyzed for crude protein (CP), neutral detergent fiber (NDF), and NO3– N. In grain-bearing accessions, leaf CP content increased with N application but declined linearly over harvest dates. In vegetable types, leaf CP levels tended to fluctuate over time. In both types, NDF content declined with N application, whereas response to harvest date varied. Leaf NO3– increased two-fold in plants from fertilized plots compared to plants from unfertilized plots, but declined rapidly with time. Leaf content of NO3– did not exceed 239 mmol·kg–1 dry weight with any N fertilization treatment. Edible amaranth appeared to be adapted to soils and climate of the southeastern United States. A. tricolor was most susceptible to disease among the accessions evaluated.