Due to the basic topographical characteristics of the gentle and long slope lengths in the Mollisol region of Northeast China, severe soil erosion is easily aggravated by the concentration of surface flow. The spatial distribution of water depth and hydrological connectivity index were introduced to evaluate the effects of typical soil and water conservation practices on the overland flow path and hydrological connectivity based on the GIS and SIMWE (SIMulated Water Erosion) model. We analyzed the effects of different soil and water conservation practices on the hydrological connectivity, water flow path, and spatial distribution of soil erosion and sediment yield by quantifying the variations of soil infiltration rate and surface manning roughness, as well as by constructing an artificial terrain digital elevation model (DEM). The results showed that: 1) terraces could effectively affect the hydrological connectivity of the slope and regulate flow path, with significant differences between the responses of hydrological connectivity and flow path under different forms of terraced fields and ridges. The characteristics of spatial distribution of soil erosion and sediment yield varied with changes in water flow path, which would eventually lead to the intensification of local erosion; 2) practices of vegetated buffer strips and contour tillage presented limited effectiveness on runoff path controlling, though they played a significant role in sediment retention; and 3) conservation tillage could reduce the hydrological connectivity and improve the retention capacity of runoff by increasing surface roughness. This study quantified the effects of different soil and water conservation practices on the hydrological connectivity, flow path, and spatial distribution of soil erosion and sediment yield, and could provide a theoretical reference for scientific layout of soil and water conservation practices in black soil region.