Floor Soil Research Articles

Elevated blood lead and metal/metalloid levels and environmental exposure sources in urban Ecuadorian school-age children and mothers.

Lead and other toxic and potentially toxic metals and metalloids are significant contributors to the global burden of disease and disability. Studies characterizing blood metal/metalloid levels and potential sources of environmental exposures are limited for populations living in the major urban centers of Andean-area countries. We used ICP-MS to quantify blood levels of lead (PbB), cadmium (CdB), manganese (MnB), total arsenic (AsB), and total mercury (HgB) in school-age children (n=47) and their reproductive-age mothers (n=49) from low-resource households in Quito, Ecuador. These were compared to published 95th percentile reference values (RV95) and for PbB, also to CDC reference values. We used a detailed environmental questionnaire to examine the contribution of residential and neighborhood environmental exposure sources with participant blood metal/metalloid levels. We used ICP-MS to measure Pb levels in residential windowsill dust, floor dust, and drinking water samples and used XRF for paint samples. Forty-five percent of the mothers had PbBs ≥ 5μg/dL; 14.3% had PbBs ≥10μg/dL. Maternal blood levels exceeded RV95s for PbB (76%), CdB (41%), MnB (88%), HgB (57%), and AsB (90%). Of children, 68% had PbBs ≥ 5μg/dL, and 21.3% had PbB ≥10μg/dL. Most child blood levels exceeded the RV95s for PbB (100%), CdB (100%), MnB (94%), and total HgB (94%) and AsB (98%). Most mothers (97%) and all children had blood levels indicating exposure to multiple metal/metalloid mixtures. Maternal and child PbBs were moderately correlated with each other but the other four metals/metalloids were not. Factors associated with maternal blood metal/metalloid levels were residence in a home with an earthen floor (PbB) or bare cement block walls (MnB), living near a dirt-paved or cobblestone street (PbB), <50m from a heavily trafficked major roadway (PbB, HgB), living in the Los Chillos (PbB) or Cotocollao neighborhoods (PbB, HgB), or in areas where street vendors grilled food using charcoal (MnB). Factors associated with child blood metal/metalloid levels were residence in a dirt floor home (PbB, CdB, AsB), living near a dirt-paved or cobblestone street (PbB), living in the El Camal or Cotocollao neighborhoods (AsB), or in local neighborhoods where scrap metal smelters (MnB) and LPG gas depositories (PbB) were present. The elevated blood levels of PbB, other metal/metalloids, and metal/metalloid mixtures identified mothers and children in this exploratory study is an urgent public health and clinical concern. The exposure patterns suggest that traffic-related exposures, especially the resuspension of legacy Pb in dust, as well as other anthropogenic and geogenic sources may be important environmental contributors to metal/metalloid exposures in urban Ecuadorian mothers and children. Future studies are needed to confirm these findings and explore other potential exposure sources. Biomonitoring is also needed in order to formulate effective intervention strategies to reduce population exposure to toxic levels of environmental metals/metalloids.

Urban ecology of hosts and vectors of Rickettsia in a rickettsiosis-endemic city of the Yucatan peninsula, Mexico

Rickettsioses are vector-borne zoonotic diseases that occur in urban environments. Currently, they are associated with the presence of domestic and synanthropic animals, the ectoparasites that they harbor, and their local habitat. The implementation of prevention actions relies on the understanding of the local ecology of interactions between hosts, vector species, and the etiologic agents. In this context, this study aimed to explore and describe the occurrence of infected mammals and their ectoparasites in human urban dwellings, and those characteristics of urban dwellings associated to the presence of Rickettsia infected animals in groups of households where at least one human case of rickettsiosis has occurred in the previous year of the study. Briefly, blood-samples and ectoparasites from synanthropic and domestic animals, were obtained from groups of households from different areas of an urban settlement. Serologic and molecular diagnostics helped to identify Spotted Fever Group (SFG) and TG (Typhus Group) Rickettsia in animal and ectoparasite samples. A total of 99 mammals were sampled, 29 opossums (Didelphis virginiana), 13 house mice (Mus musculus), seven black rats (Rattus rattus) and 50 dogs. Infection occurrence in opossums was 8.3% of SFG, 50% for TG, and 4.2% of undetermined group. For house mice 46.2% for SFG and 30.8% were undetermined. Black rats 28.6% of SFG and 57.1% undetermined. Finally, dogs were 19.1% of SFG, 57.4% to TG, and 23.4% belonged to undetermined group. A total of 424 ectoparasites were collected from the mammals. In opossums occurred the ticks Ambyomma sp., Ornithodoros (Alecterobius) nr. talaje, and the flea Ctenocephalides felis. In dogs we found the ticks Rhipicephalus sanguineus s. l., Amblyomma sp., O. (A.) nr. talaje, and the flea Ct. felis. No ectoparasites were collected from rodents. The occurrence of infected animals was associated primarily with the material of the backyard floor, the type of sanitary system in the household, the presence of garbage in the backyard, presence of firewood storage, stored polyethylene terephthalate (PET) containers for sale to recyclers, and the store of construction supplies in the backyard. Nonetheless a generalized linear model showed that the household with a backyard with a dirt floor or other non-concrete material has more chances of harboring infected animals (RR= 1.74, 95% CI= 1.07-2.84 and RR= 1.03, 95% CI= 0.39-2.32 respectively). In contrast, when the house has a sanitary system of urban sewer system or a latrine outside de house, the chances of having infected animals decreased significantly (RR= 0.39, 95% CI= 0.12-0.94 and RR= 0.46, 95% CI= 0.03-2.22). We conclude that both SFG and TG rickettsioses occur in animals and their ectoparasites in peridomiciles of urban households were at least one human rickettsiosis case had occurred.

Empirical Path Loss Channel Characterization Based on Air‐to‐Air Ground Reflection Channel Modeling for UAV‐Enabled Wireless Communications

The purpose of this work was to investigate the air‐to‐air channel model (A2A‐CM) for unmanned aerial vehicle‐ (UAV‐) enabled wireless communications. Specifically, a low‐altitude small UAV needs to characterize the propagation mechanisms from ground reflection. In this paper, the empirical path loss channel characterizations of A2A ground reflection CM based on different scenarios were presented by comparing the wireless communication modules for UAVs. Two types of wireless communication modules both WiFi 2.4 GHz and LoRa 868 MHz frequency were deployed to study the path loss channel characterization between Tx‐UAV and Rx‐UAV. To investigate the path loss, three types of experimental channel models, such as CM1 grass floor, CM2 soil floor, and CM3 rubber floor, were considered under the ground reflection condition. The analytical A2A Two‐Ray (A2AT‐R) model and the modified Log‐Distance model were simulated to compare the correlation with the measurement data. The measurement results in the CM3 rubber floor scenario showed the impact from the ground reflection at 1 m to 3 m Rx‐UAV altitudes both 2.4 GHz and 868 MHz which was converged to the A2AT‐R model and related to the modified Log‐Distance model above 3 m. It clear that there is no ground reflection effect from the CM1 grass floor and CM2 soil floor. This work showed that the analytical A2AT‐R model and the modified Log‐Distance model can deploy to model the path loss of A2A‐CM by using WiFi and LoRa wireless modules.

Dynamics in Stoichiometric Traits and Carbon, Nitrogen, and Phosphorus Pools across Three Different-Aged Picea asperata Mast. Plantations on the Eastern Tibet Plateau

Understanding the variations in soil and plants with stand aging is important for improving management measures to promote the sustainable development of plantations. However, few studies have been conducted on the dynamics of stoichiometric traits and carbon (C), nitrogen (N), and phosphorus (P) pools across Picea asperata Mast plantations of different ages in subalpine regions. In the present study, we examined the stoichiometric traits and C, N, and P stocks in different components of three different aged (22-, 32-, and 42-year-old) P. asperata plantations by plot-level inventories. We hypothesized that the stoichiometric traits in mineral soil could shape the corresponding stoichiometric traits in soil microbes, tree roots and foliage, and the C, N, and P stocks of the total P. asperata plantation ecosystem would increase with increasing stand age. Our results show that the N:P ratio in mineral soil was significantly correlated with that in tree foliage and herbs. Additionally, the C:N ratio and C:P ratio in mineral soil only correlated with the corresponding stoichiometric traits in soil microbes and forest floor, respectively. Both the fractions of microbial biomass C in soil organic C and microbial biomass N in soil total N decreased with increasing stand age. The C, N, and P stocks of the total ecosystem did not continuously increase across stand development. In particular, the P stock of the total ecosystem exhibited a trend of increasing first and then decreasing. The aboveground tree biomass C accounted for more than 55% of the total ecosystem C stock regardless of stand age. In contrast, mineral soil and forest floor were the major contributors to the total ecosystem N and P stocks in all stands. This study suggested that all three different stands were N limited, and the stoichiometric homeostasis in the roots of P. asperata was more stable than that in the foliage. In addition, the soil microbial community assembly may change with increasing stand age for P. asperata plantations in the subalpine region.

Socio-demographic and environmental factors associated with diarrhoeal disease among children under five in India

BackgroundGlobally, diarrhoea is the second leading cause of death in children under five and a major public health problem. Despite several health care initiatives taken by the government, a large proportion of children still experience diarrhoeal diseases which cause high childhood death in India. This study aims to examine the socio-demographic and environmental factors associated with diarrhoea in children under five in India.MethodsA cross-sectional study was designed using secondary data from the recent round of the National Family Health Survey (NFHS-4), conducted in 2015–16. A total of 247,743 living children below 5 years of age were included in the analysis. Bivariate and multivariate logistic regression models were carried out to assess the factors associated with childhood diarrhoeal disease.ResultsIn India, about 9% of under-five children experience diarrhoeal disease in the past 2 weeks preceding the survey. Children living in rural areas (Adjusted odds ratio [aOR]: 1.05; 95% CI: 1.01, 1.09), children belonged to scheduled tribe (aOR: 0.83; 95% CI: 0.79, 0.89) and other castes (aOR: 0.92; 95% CI: 0.88, 0.97), Muslim children (aOR: 1.18; 95% CI: 1.13, 1.24), and children resided in the central (aOR: 1.61; 95% CI: 1.52, 1.70) and west (aOR: 1.08; 95% CI: 1.01, 1.15) regions were significantly associated with higher likelihood of diarrhoea in the past 2 weeks. Concerning environmental factors, child stool disposal (aOR: 1.06; 95% CI: 0.98, 1.09), floor materials (aOR: 1.08; 95% CI: 1.03, 1.12) and roof materials (aOR: 1.08; 95% CI: 1.04, 1.13) of the household were found to be significant predictors of childhood diarrhoea occurrence.ConclusionsDiarrhoeal disease is common among children who lived in rural areas, scheduled castes, Muslims, and children from poor families. Regarding environmental factors, stool disposal practices in the household, dirt floor, and thatch roof materials of the household unit are risk factors for diarrhoeal disease. Targeted approach should be initiated to mitigate the problem of the poor health status of children by providing adequate health care. The policy-makers and stakeholders should address adverse environmental conditions by the provision of latrine and improved housing facilities.

Composition of soil bacterial and fungal communities in relation to vegetation composition and soil characteristics along an altitudinal gradient.

The objective of the present study was to evaluate how altitudinal gradients shape the composition of soil bacterial and fungal communities, humus forms and soil properties across six altitude levels in Hyrcanian forests. Soil microbiomes were characterized by sequencing amplicons of selected molecular markers. Soil chemistry and plant mycorrhizal type were the two dominant factors explaining variations in bacterial and fungal diversity, respectively. The lowest altitude level had more favorable conditions for the formation of mull humus and exhibited higher N and Ca contents. These conditions were also associated with a higher proportion of Betaproteobacteria, Acidimicrobia, Acidobacteria and Nitrospirae. Low soil and forest floor quality as well as lower bacterial and fungal diversity characterized higher altitude levels, along with a high proportion of shared bacterial (Thermoleophilia, Actinobacteria and Bacilli) and fungal (Eurotiomycetes and Mortierellomycota) taxa. Beech-dominated sites showed moderate soil quality and high bacterial (Alphaproteobacteria, Acidobacteria, Planctomycetes and Bacteroidetes) and fungal (Basidiomycota) diversity. Particularly, the Basidiomycota were well represented in pure beech forests at an altitude of 1500m. In fertile and nitrogen rich soils with neutral pH, soil quality decreased along the altitudinal gradient, indicating that microbial diversity and forest floor decomposition were likely constrained by climatic conditions.

Managing botulism outbreaks associated with poultry litter: balancing perceived animal welfare with disease prevention

Should we rear animals outside to allow them to better express natural behaviours, or should we keep them indoors to protect them from pathogens that cause pain and distress? The...

Comparable canopy and soil free-living nitrogen fixation rates in a lowland tropical forest

Biological nitrogen fixation (BNF) is a fundamental part of nitrogen cycling in tropical forests, yet little is known about the contribution made by free-living nitrogen fixers inhabiting the often-extensive forest canopy.We used the acetylene reduction assay, calibrated with 15N2, to measure free-living BNF on forest canopy leaves, vascular epiphytes, bryophytes and canopy soil, as well as on the forest floor in leaf litter and soil. We used a combination of calculated and published component densities to upscale free-living BNF rates to the forest level.We found that bryophytes and leaves situated in the canopy in particular displayed high mass-based rates of free-living BNF. Additionally, we calculated that nearly 2 kg of nitrogen enters the forest ecosystem through free-living BNF every year, 40% of which was fixed by the various canopy components.Our results reveal that in the studied tropical lowland forest a large part of the nitrogen input through free-living BNF stems from the canopy, but also that the total nitrogen inputs by free-living BNF are lower than previously thought and comparable to the inputs of reactive nitrogen by atmospheric deposition.

Effects of afforestation with different species on carbon pools and soil and forest floor properties

Land use and land use change are factors that affect carbon and nutrient stocks in ecosystems. The aim of this research was to determine the effects of forest land use types on carbon pools and soil and forest floor features. This study was conducted in areas afforested with black pine (Pinus nigra Arn. subsp. pallasiana Lamb. Holmboe) and Scots pine (Pinus sylvestris L.) and on adjacent bare land within the Akdağ Nature Park, which is located in the West-Central Anatolia Region of Turkey. Three 20 × 20 m sample plots were selected within each forest land use, and the diameters at breast height and heights of all trees were measured. Tree biomass and carbon stocks in the unit areas were calculated using tree biomass equations, and carbon conversion factors were developed for two tree species. Within each sample plot, disturbed and core soil samples and forest floor samples were taken at three points at depths of 0–10, 10–20 and 20–30 cm. The physical and chemical properties of the soil and forest floor samples were determined in the laboratory and measurements were converted to a unit area using volume values. The data were evaluated using independent sample t-tests and analysis of variance. The results showed that the ecosystem carbon (C) stocks differed significantly with forest land use type; black pine plantations, Scots pine plantations and bare land accumulated 235.2 t C ha−1, 206.1 t C ha−1 and 37.4 t C ha−1, respectively. We found that in addition to the positive effect of afforestation on soil, black pine had a greater impact on some forest floor and soil characteristics in the region than Scots pine. Thus, we suggest that priority should be given to black pine in afforestation of the region and in other ecosystems with similar climates. Additionally, our results reveal the importance of afforestation on bare lands for reducing the impact of global climate change.

Effect of plastic slatted flooring on growth and welfare of stall-fed kids

Male kids (36) at 6 months age, 12 each of Jamunapari, Jakhrana, Barbari breed were equally divided and allotted randomly in plastic and soil floor and reared up to 12 of age through April to September months, fed ad lib. roughage, green fodder and concentrate to meet nutrient requirements for optimum growth. Body weight was recorded fortnightly, physiological responses were recorded twice and blood sampling at 60th and 120th day of trial. The weight gain and ADG on plastic floor was similar as compared to soil floor. Kids were equally comfortable on both floors except RR; however, increased RR on plastic floor did not affect the weight gain in kids. Plasma AST and ALT enzymes, total protein, albumin, total cholesterol, triglycerides level differed non-significantly in kids reared on both plastic and soil floor indicating the suitability of plastic floor in goat shelters in semi-arid conditions.

Behavioral responses and reproductive performances of cross bred dairy cows on soil and concrete bedded floors in small holders’ production system in Assam

The behavioral response to the selected micro environment is one of the important tools for scientific assessment of welfare of the dairy animals. The study was conducted in 5 number of soil bedded sheds and 5 number of concrete bedded sheds in Kamrup (Metro) District of Assam to assess the behavioral responses of the cross bred dairy cattle in small holders’ production system. From each shed 5 number of dairy cows were selected preferably of 1-2 months of lactation stage. The selected animals were observed for three occasions at 30 days interval for 3 hours daily on 12 hours basis. The time spent (Mean±SE) for rumination, resting and sleeping were recorded as 184.60±1.54, 262.70±0.87 and 53.78±1.38 minutes and 190.40±1.50, 265.60±0.72 and 49.40±1.26 respectively. There was a highly significant difference between both the types of floor (P≤ 0.01) for rumination and resting time. The overall prevalence of post parturition problems were higher (40%) in soil bedded floor than that of concrete bedded floor (20%). The animal welfare issues were compromised on the soil bedded floor type in comparison to concrete bedded floor type of shed.

Post-Harvest Handling Practices Impacted on Microbiological and Sensory Quality Attributes of Giant Freshwater Prawn Macrobrachium Rosenbergii in the Distribution Chain in Bangladesh

To understand the effect of post-harvest good handling practices on prawn(Macrobrachiumrosenbergii) quality, data were collected from stakeholders involved indistribution chain by semi-structured questionnaire, focus group discussion and transectwalking at Kishoreganj and Mymensingh. Most used containers were split bamboo basket(39%), split bamboo basket with polythene lining (18%), plastic crate (12%), styrofoamcrate (8%) and rectangular metal container (12%). 15%, 25%, 10%, 100% and 7% largeprawns were water washed and 9%, 35%, 22%, 100%, 60% large prawns were icedimmediately after harvest, later in fishing trip, immediately after landing, at wholesalehouse before transportation and during retail sale, respectively. Bacterial counts of waterused for ice preparation, ice crushed mechanically and on soil floor collected from BhairabBazar and Karimganj were 6.58±1.87×103, 4.77±0.19×102, 4.12±2.06×103 cfu/ml and4.82±1.56×102, 6.01±1.61×101, 6.90±1.61×102 cfu/ml, respectively. Ice melted watercollected after unpacking prawn containers at Mymensingh, transported from BhairabBazar and Karimganj, had 5.90±1.95×107 and 6.32±2.06×108 cfu/ml bacterial counts,respectively; indicated unhygienic handling environments. However, existing post-harvesthandling practices produce prawns acceptable for consumers as sensory assessment forprawns showed the quality defect points of 1.0 (Excellent), 2.1 (Good), 2.3 (Good) and 2.9(Good) at harvesting, landing, wholesale and retail shop, respectively.&#x0D; Res. Agric., Livest. Fish.7(2): 321-328, August 2020

Market integration and soil-transmitted helminth infection among the Shuar of Amazonian Ecuador.

BackgroundSoil-transmitted helminth (STH) infections have many negative health outcomes (e.g., diarrhea, nutritional deficiencies) that can also exacerbate poverty. These infections are generally highest among low-income populations, many of which are also undergoing market integration (MI; increased participation in a market-based economy). Yet the direct impact of MI-related social and environmental changes on STH infection patterns is poorly understood, making it unclear which lifestyle factors should be targeted to better control disease spread. This cross-sectional study examines if household infrastructure associated with greater MI is associated with lower STH burdens among Indigenous Ecuadorian Shuar.MethodsKato-Katz fecal smears were used to determine STH infection status and intensity (n = 620 participants; 308 females, 312 males, aged 6 months—86 years); Ascaris lumbricoides (ascarid) and Trichuris trichiura (whipworm) were the primary infection types detected. Structured interviews assessing lifestyle patterns (e.g., measures of household infrastructure) measured participant MI. Multilevel regression analyses and zero-inflated negative binomial regression models tested associations between MI measures and STH infection status or intensity, controlling for individual and community characteristics.ResultsParticipants residing in more market-integrated households exhibited lower infection rates and intensities than those in less market integrated households. Parasite infection status and T. trichiura infection intensity were lower among participants living in houses with wood floors than those with dirt floors, while individuals using well or piped water from a spring exhibited lower A. lumbricoides infection intensities compared to those using river or stream water. Unexpectedly, latrine type was not significantly related to STH infection status or intensity. These results suggest that sources of exposure differ between the two helminth species.ConclusionsThis study documents associations between household measures and STH infection among an Indigenous population undergoing rapid MI. These findings can help healthcare programs better target interventions and reduce STH exposure among at-risk populations.

Seasonal variation of microbial activity in soil and forest floor under three different fir plantations

Microbil activity is one of the important process for biochemical cycles in soil and forest floor of ecosystems. Because, , the carbon dioxide and nutrients needed by plants are released during the microbial activity. In this study, we were investigated releationships between environmental factors (mositure, temperature, pH, electric conductivity, C, N, Na, Ca, Mg, K, P) and seasonal variations of microbal respiration, microbial biomass-C and metabolic quotient (qCO2) in the forest floor and soil (0-5cm) under three adjacent Abies plantation plots (Abies nordmanniana ssp. bornmuelleriana Mattf. (Ab), Abies cilicica Carr. (Ac) and Abies nordmanniana ssp. nordmanniana Mattf (An)) in Atatürk Arboretum located in Istanbul-Turkey. Sampling was carried out by collecting bimonthly (from May-2012 to March-2013) both soil (54 sample) and forest floor (54 sample) samples within each species. According to the results of the study, soil microbial respiration (SMR) has significantly lower value in Ab plot. Although SMR and soil microbial biomass-C (SMBC) were correlated with moisture and temperature in An plot, they were correlated with nutrients in other plots. In general, an increase in soil respiration rates was showed in autumn and early spring. Forest floor microbial respiration (FFMR), microbial biomass-C (FFMBC) and metabolic quotent (qCO2) did not differ among the plots. The measured FFMR, FFMBC and qCO2 parameters were lower in autumn than spring. Forest floor microbial parameters were drived by the variation of nutrients amounts. As a result, microbial processes in both soil and forest floor changed with the effect of different factors although there was no clear difference among the plots,

Novel Evolutionary Algorithm Identifies Interactions Driving Infestation of Triatoma dimidiata, a Chagas Disease Vector.

Chagas disease is a lethal, neglected tropical disease. Unfortunately, aggressive insecticide-spraying campaigns have not been able to eliminate domestic infestation of Triatoma dimidiata, the native vector in Guatemala. To target interventions toward houses most at risk of infestation, comprehensive socioeconomic and entomologic surveys were conducted in two towns in Jutiapa, Guatemala. Given the exhaustively large search space associated with combinations of risk factors, traditional statistics are limited in their ability to discover risk factor interactions. Two recently developed statistical evolutionary algorithms, specifically designed to accommodate risk factor interactions and heterogeneity, were applied to this large combinatorial search space and used in tandem to identify sets of risk factor combinations associated with infestation. The optimal model includes 10 risk factors in what is known as a third-order disjunctive normal form (i.e., infested households have chicken coops AND deteriorated bedroom walls OR an accumulation of objects AND dirt floors AND total number of occupants ≥ 5 AND years of electricity ≥ 5 OR poor hygienic condition ratings AND adobe walls AND deteriorated walls AND dogs). Houses with dirt floors and deteriorated walls have been reported previously as risk factors and align well with factors currently targeted by Ecohealth interventions to minimize infestation. However, the tandem evolutionary algorithms also identified two new socioeconomic risk factors (i.e., households having many occupants and years of electricity ≥ 5). Identifying key risk factors may help with the development of new Ecohealth interventions and/or reduce the survey time needed to identify houses most at risk.

Prescribed burn frequency, vegetation cover, and management legacies influence soil fertility: Implications for restoration of imperiled pine barrens habitat

In fire-dependent ecosystems, the absence of fire can contribute to a positive feedback in which increased vegetation cover leads to increased accumulation of soil organic matter and nutrient stocks. These changes in turn can influence competitive shifts among plant communities, resulting in increased woody plant establishment, canopy closure, and ultimately leading toward mesophication. Poor soil conditions may be especially important for maintaining the open structure characteristic of pine barrens ecosystems, which are imperiled due to loss of key ecosystem processes such as fire and land conversion to cropland or pine plantations. Our objectives were to determine how soil characteristics are related to recent prescribed fire management, and how soils vary along gradients of current and historic vegetation cover in a barrens-forest mosaic in northern Wisconsin, USA. We sought to understand whether management with prescribed fire shifts soils toward barrens-type soil conditions, and whether soil conditions typify barrens habitat relative to shrub and forest habitat We analyzed organic (i.e. forest floor) and mineral soil horizons collected along gradients of recent prescribed burn history and current (barrens, pine woodlands, brush, and closed-canopy forest) and historic vegetation cover types (barrens, pine plantations, deciduous forests) to investigate the influence of each on specific components of soil fertility. Using a model selection approach, we found forest floor soil properties were most frequently associated with differences in current vegetation cover; for instance, pine woodland sites had greater organic matter stocks than barrens sites, and cation stocks were generally greater at brush sites than barrens and pine woodland sites. Some soil properties, including pH, however, appeared to be driven by prescribed burn frequency. Using ordination techniques to characterize multidimensional characterizations of soils, we identified a soil legacy effect related to historic vegetation cover and land management; native barrens sites had soil characteristics intermediate to restored barrens of pine woodland and deciduous forest origin. Our findings suggest restoration of pine plantations to barrens could benefit from fire activities that enhance consumption of the forest floor, while restoration of deciduous forest and brush habitats will likely be more related to the effective control of hardwood regeneration, after which soil conditions may return to a more archetypical barrens state.

Application of the Karst Disturbance Index (KDI) to Kobokwe Cave and Gorge, SE Botswana: Implications for the Management of a Nationally Important Geoheritage Site

Geoheritage sites can provide an excellent resource for sustainable development that represents a growing movement in Africa. Kobokwe Cave is formed within the Middle Proterozoic–aged sandstone of Kobokwe Gorge and is nationally important; the cave is the site where British missionary David Livingstone spent a night in 1847 and subsequently converted Kgosi (Chief) Kgari Sechele I, the first and only Christian “conversion” at that time by Livingstone. However, the site currently appears undervalued as there are little stewardship and minimal organized tourism and is affected by litter, graffiti, fires, illegal dumping and sand mining, and noise pollution from a nearby road; the building of the road itself destroyed a boulder of cultural significance. To test the level of environmental disturbance, the Karst Disturbance Index (KDI) was employed and this is the first known application to a non-carbonate karst environment and the first such application in Africa. Field observations were coupled with laboratory analyses of physico-chemical, nitrate, and heavy metal parameters in soil and water from the site. The final KDI score for Kobokwe of 0.29 is classed as showing “minor disturbance” and likely due to low visitor numbers although the sub-category of cultural disturbance scored 12 and is relatively high, as also found in previously published KDI studies from the USA, Italy, and Mexico. The analytical results are mostly at natural background levels, except for Pb in the cave floor soil (5.13 mg/L) and Pb in all water samples (0.3–0.63 mg/L) likely due to nearby vehicular traffic; also, Fe in all water samples (0.79–1.13 mg/L) that has possibly leached from the gorge sandstone and the spring water was slightly above standard background levels and possibly leached from nearby soil/rock. Future recommendations to avoid further disturbance and to promote environmental management of the site include community involvement, litter bin installation, graffiti removal, tour guides, information panels, entrance gate, tarred road, footpath installation, and enforcing of stronger site stewardship.

Nicaraguan Homeowner Showing Gratitude for Concrete Flooring as Part of a Public Health Mission Trip.

Description I'm Bree Kinnison, a fourth-year medical student and aspiring psychiatrist. When I'm not studying, I enjoy painting using acrylics. This painting depicts a very fond memory of mine. After completing the medical portion of our mission trip in Nicaragua, we began our public health portion. Along with fellow students, I laid concrete flooring in this woman's house. For all 76 years of her life, she had never experienced anything other than a dirt floor in her home. Traditional dirt flooring in Nicaraguan homes is responsible for many preventable illnesses. When shown the final product, she reached out and hugged the first person she could get ahold of. She began crying and thanking God for placing us in her life. We were equally grateful for this life-changing experience.

Effect of floor on welfare of lactating cows in small farms of Sirajgonj district, Bangladesh

Animal welfare is a relatively new topic and there is a dearth of the report regarding the welfare assessment of dairy cattle in Bangladesh. Therefore, the present study was carried out to assess the effect of floor surface of the dairy sheds on mastitis, skin lesions, cleanliness of body and lameness of lactating cow in smallholders’ production system. To conduct this study, 5 soil type floor, 5 brick paved and 5 concrete floors of small holder dairy units were selected in Sirajgonj district. From each shed, 5 numbers of dairy cows were selected preferably in the first lactation stage. A total 75 milk samples from three different types of the shed were tested for subclinical mastitis using a California mastitis test. The results showed that 40%, 28%, and 32% cows were CMT positive for subclinical mastitis in soil, brick made and concrete type floor, respectively. The prevalence of SCM was not significantly differences among the different types of floor (P&gt;0.05). Cleanliness and hygienic status of the muddy soil type floor were found to be significantly poor in comparison to that of brick made and concrete type floor of dairy farms. The prevalence of skin lesions in different body parts was higher in brick made (62%) and concrete floor sheds (88%) compared to soil bedded (31%) floor. Hair loses in the concrete and brick made floor was significantly higher (P≤0.01) in comparison with the muddy soil floor. The overall prevalence of lameness of the brick made (72%) and concrete type floor was higher (65%) than that of soil bedded shed (48%). There was a significant difference of lameness between soil type and brick made floor of the shed (P&gt;0.05). The study recommends that in order to reduce the high prevalence of subclinical mastitis, skin lesions and lameness in smallholder farmers require to improve floor conditions through regular cleaning of the floor or upgrade to the concrete floor using rubber mat.&#x0D; Res. Agric., Livest. Fish.7(1): 87-95, April 2020

Factors associated with anaemia in a nationally representative sample of nonpregnant women of reproductive age in Nepal.

We used cross‐sectional data from the 2016 Nepal National Micronutrient Status Survey to evaluate factors associated with anaemia among a nationally representative sample of nonpregnant women 15– 49 years (n = 1, 918). Haemoglobin, biomarkers of iron status and other micronutrients, infection, inflammation, and blood disorders were assessed from venous blood. Soil‐transmitted helminth and Helicobacter pylori infections were assessed from stool. Sociodemographic, household, and health characteristics and diet were ascertained by interview. We conducted bivariate analyses between candidate predictors and anaemia (haemoglobin <12.0 g/ dL, altitude‐ and smoking‐adjusted). Candidate predictors that were significant in bivariate models (P < 0.05) were included in the multivariable logistic regression model, accounting for complex sampling design. Anaemia prevalence was 20.2% (95% confidence interval [CI] [17.6, 22.8]). Associated with reduced anaemia odds were living in the Mountain and Hill ecological zones relative to the Terai (adjusted odds ratio [AOR] 0.35, 95% CI [0.21, 0.60] and AOR 0.41, 95% CI [0.29, 0.59], respectively), recent cough (AOR 0.56, 95% CI [0.38, 0.82]), hormonal contraceptive use (AOR 0.58; 95% CI [0.38, 0.88]), ln ferritin (micrograms per litre; AOR 0.43, 95% CI [0.35, 0.54]), and ln retinol binding protein (micrograms per litre; AOR 0.20, 95% CI [0.11, 0.37]). Residing in a house with an earth floor (AOR 1.74, 95% CI [1.18, 2.56]), glucose‐6‐ phosphate dehydrogenase deficiency (AOR 2.44, 95% CI [1.66, 3.60]), and haemoglobinopathies (AOR 6.15, 95% CI [3.09, 12.26]) were associated with increased anaemia odds. Interventions that improve micronutrient status, ensure access to hormonal birth control, and replace dirt floors to reduce infection risk might help reduce anaemia in this population.