Soil Fertility Research Articles

Organic fertilizer integrated with marine waste derived CaCO3 nanocarrier system: A focus on enhanced yield and quality in tomato cultivation

Tomatoes are rich in lycopene, β-carotene, ascorbic acid and other mineral sources including phosphorus, potassium, zinc, magnesium and iron. Major constraints in tomato cultivation were high cost, poor cultivation due to adverse weather conditions, pest attacks, microbial infections and nutritional deficiency complications. Conventional fertilizers, pesticides, fungicides and growth regulators are effective at higher concentration, which induces specific toxic effects on soil fertility, plant yield and also affects the health status of humans, animals and soil associated microbes. The use of organic fertilizers to meet the soil nutrient demand increases the acidity of soil affecting plant growth, which turned the focus of researchers towards nanofertilizer. The present study focuses on synthesis of marine waste derived CaCO3 nanoparticles formulated with azadirachtin and panchakavya emulsion to develop a CaCO3 nanofertilizer. CaCO3 nanofertilizer developed through this study was investigated for its material properties and behavioral traits. Further, the in-vitro antifungal impact of the CaCO3 nanofertilizer was examined, and it was sprayed on tomato plants via foliar spray. CaCO3 nanofertilizer effectively inhibited fusarium wilt causing plant fungal pathogen and also exhibited enhanced growth and yield of tomatoes against pest attack and nutritional deficiency with effect to foliar treatment. Also, CaCO3 nanofertilizer enhanced the total carotenoid level and essential nutritional minerals in fruit yield of tomatoes. Overall, fabricated CaCO3 nanofertilizer exhibits synergistic role of fertilizer, pesticide, fungicide and growth regulator in tomato cultivation. It suggests that, CaCO3 nanofertilizer generated from renewable biowaste will become the innovative platform for sustainable agriculture.

Soil organic carbon, carbon fractions, and microbial community under various organic amendments

The impact of various organic amendments on soil organic carbon (SOC) have rarely been reported. To address this, a laboratory experiment was designed to scrutinize the effects of different amendments on soil carbon fractions, microbial communities, and the underlying interactive mechanisms. The experiment encompassed a no-amendment control (CK), as well as treatments with corn straw (CS), tobacco stalks (TS), and peanut shell biochar (PB). Over a 70-day incubation, the SOC in plots amended with CS, TS, and PB displayed significant boosts of 13.9%, 17.5%, and 44.8%, respectively, compared to the CK. For soil carbon fractions, amendments with PB, TS, and CS led to a dramatic rise in particulate organic carbon (POC) of 27.4%, 20.2%, and 105.7%, respectively, in contrast to the CK plots. Mantel analysis and structural Equation Modeling uncovered strong interrelationships among the cbbL, cbbM, Bacteroidota, TOC, and POC. Organic amendments enhance soil carbon fractions, modulating the microbial community by increasing Bacteroidetes abundance and suppressing Acidobacteria richness, thereby influencing the abundance of key carbon cycle genes such as cbbL and cbbM. These results suggest that the addition of peanut shell biochar significantly boosted TOC and key carbon fractions, enhancing carbon content and soil fertility.

Relationships between abiotic factors, foliage chemistry and herbivory in a tropical montane ecosystem.

Herbivore-plant interactions are fundamental processes shaping ecosystems, yet their study is challenged by their complex connections within broader ecosystem processes, requiring a nuanced understanding of ecosystem dynamics. This study investigated the relationship between nutrient availability and insect herbivory in the Australian Wet Tropics. Our objectives were threefold. Firstly, to understand what factors influence nutrient availability for plants and herbivores across the landscape; secondly, to investigate how trees of different species respond to nutrient availability; and thirdly, to unravel how the relationships between resources and plant chemistry affect herbivory. We established a network of 25 study sites covering important abiotic gradients, including temperature, precipitation, and geology. Employing a hierarchical modelling approach, we assessed the influence of climate and geology on resource availability for plants, primarily in the form of soil nutrients. Then, we explored the influence of the above factors on the interaction between herbivory and foliage chemistry across three widespread rainforest tree species, comparing how these relationships emerged across genera. Our findings suggest an overarching influence of climate and geology over soil chemistry, foliar nitrogen, and insect herbivory, both directly and indirectly. However, individual constituents of soil fertility showed equivocal influences on spatial patterns of foliage chemistry once site geological origin was accounted for, suggesting a questionable relationship between individualsoil nutrients and foliar composition. We have demonstrated that herbivore-plant interactions are complex dynamics regulated by an intricate web of relationships spanning different biogeochemical processes. While our results provide some support to the notion that herbivory is affected by resource availability, different species growing under the same conditions can show differing responses to the same resources, highlighting the importance of identifying specific limiting factors rather than simpler proxies of resource availability.

Short-term effects of co-applied biochar and paper mill biosolids on soil microbial communities under field conditions

Biochar and paper mill biosolids (PB) are reported to improve soil fertility, crop growth and indirectly conditions for soil microbial communities. However, it is unclear how the co-application of these materials impacts soil microbial communities under field conditions. A study was initiated in 2018 in Québec, QC, Canada and fine roots and rhizosphere soil were sampled to determine the effects of co-application of wood biochar (0, 10, and 20 Mg dry wt. ha−1) and PB (0 and 30 Mg wet wt. ha−1) on the percentage of corn (Zea mays L.) and soybean (Glycine max L. Merr.) root colonization by arbuscular mycorrhizal fungi (AMF), soil microbial biomass phosphorus (MBP) and microbial (Bacteria, Fungi and AMF) diversity and community structure in a temperate loamy soil. Co-applying PB and biochar increased soil MBP compared with the control and biochar-only application. Applying PB alone or with biochar increased the level of root colonization by AMF compared with the control and biochar-only application in soybean but not in corn. Overall, biochar and PB application had no significant effect on bacterial and AMF diversity and community structure compared with the control. However, applying PB alone or with biochar decreased the fungal alpha diversity (Shannon and Simpson indices), affected several fungal taxa abundances and shifted the fungal community structure as indicated by the principal coordination analysis (PCoA). Our results provided an understanding on the short-term effects of co-applied wood biochar and PB on microbial communities of a temperate loamy soil under field conditions, as well as scientific bases for further investigation.

The Response of Inorganic and Organic Fertilizers on Growth, Yield and Quality of Cluster Bean [Cyamopsis tetragonoloba (L.) Taub.] in the Village Chikkenahalli of Challakere Taluk, Chitraduraga District

Cluster bean is one of the significant yet underutilized leguminous vegetables belonging to family fabaceae. It is known by various names across the country such as gaur, chavli kaayi, gori kaayi, khutt, govar, and kothavare. A field experiment on cluster bean was carried out during the summer season of the year 2024 at the farm of P. L. Dodda Rangegowda in Chikkenahalli of Challakere taluk, Chitradurga district, Karnataka. The primary objectives of the study are to examine the effects of organic and inorganic fertilizers on the productivity and growth characteristics of cluster bean; to identify the advantages and disadvantages of these fertilizers in relation to productivity, soil fertility, and environment and further to review the effects of mixed organic and inorganic fertilizers on yield and soil fertility. The experiment was laid out in a randomized block design with four replications and five treatments which comprised of 100 percent organic (FYM and chicken manure), 100 percent inorganic (NPK), 50 percent organic + 50 percent inorganic, 100 percent organic + jeevamruta and control. The results revealed that, treatment T3 outperformed the others, showing the highest values for plant height, number of branches per plant, days to first flowering, days to first vegetable harvest, pod length, pod width, yield per plant, yield per plot, and yield per hectare, while the control recorded the lowest values. Soil properties such as pH, EC, SOC and NPK levels improved when combination of organic and inorganic fertilizers were applied together. Based on these results, it can be concluded that, the application of organic manures, along with chemical fertilizers, positively enhanced the vegetative growth, reproductive growth, yield, quality of cluster bean and soil parameters.

Assessing the Integrated Effect of Organic and Inorganic Amendments on Soil Fertility and Rapeseed (Brassica napus L.) Yield

Assessing the Integrated Effect of Organic and Inorganic Amendments on Soil Fertility and Rapeseed (Brassica napus L.) Yield

Evaluating the impacts of area closure on soil properties in south central highland of Ethiopia

Soil degradation affects its quality and productivity of the land. To control soil degradation, various soil and water conservation (SWC) measures, including area closure are implemented. Understanding the impacts of SWC measures could support planning and implementation of land management practices. This study aimed to investigate impacts of area closure on soil properties in south central highland of Ethiopia. To acquire the necessary data, we conducted interviews with 80 households. Furthermore, nine years old area closure with physical soil and water conservation (AC-SWC), open grazing with physical SWC (OG-SWC), and open grazing without physical SWC (OG) were identified and each divided into upper, middle, and lower slope positions. In each slope position, 3 sample plots were established at 25–50 m intervals. A total of 27 composite samples and other 27 core samples for bulk density (3 treatments∗3 slope position∗3 replications) were collected and analyzed for soil properties. The data were analyzed using descriptive statistics and one-way ANOVA. The results showed that about 98 % of the respondents perceived that area closure would be an effective way to restore degraded land. Majority of the farmers (86 %) believed that area closure could reduce soil erosion and replenish soil fertility and thus, is beneficial in improving land productivity. Soil test results revealed that AC-SWC had significantly greater (P < 0.05) clay fractions, pH, available potassium, and available phosphorus than OG-SWC and OG. Variations of soil organic carbon (SOC) and total nitrogen (TN) were more pronounced. The SOC in AC-SWC was 83 % and 314 % greater than OG-SWC and OG, respectively. In the AC-SWC, TN was three and seven times higher than that of OG-SWC and OG, respectively. This could be due to vegetation restoration that reduces erosion, and enhances organic matter, moisture, and nutrient. The SOC, TN, pH, available potassium, and clay were significantly greater (P < 0.05) at lower slope position than upper in all forms of land management, which could be due to deposition at downslope. About 12 % and 11 % variations of clay fractions and SOC were, respectively, observed in between upper and lower slope positions at AC-SWC, but they varied by 25 % and 57 %, respectively, in OG. These imply area closure has had a beneficial effect on soil properties even on sloping land. AC-SWC is an important option to integrate in land management for rehabilitation of degraded soil.

A Novel Interpolation Method for Soil Parameters Combining RBF Neural Network and IDW in the Pearl River Delta

Soil fertility is a critical factor in agricultural production, directly impacting crop growth, yield, and quality. To achieve precise agricultural management, accurate spatial interpolation of soil parameters is essential. This study developed a new interpolation prediction framework that combines Radial Basis Function (RBF) neural networks with Inverse Distance Weighting (IDW), termed the IDW-RBFNN. This framework initially uses the IDW method to apply preliminary weights based on distance to the data points, which are then used as input for the RBF neural network to form a training dataset. Subsequently, the RBF neural network further trains on these data to refine the interpolation results, achieving more precise spatial data interpolation. We compared the interpolation prediction accuracy of the IDW-RBFNN framework with ordinary Kriging (OK) and RBF methods under three different parameter settings. Ultimately, the IDW-RBFNN demonstrated lower error rates in terms of RMSE and MRE compared to direct RBF interpolation methods when adjusting settings based on different power values, even with a fixed number of data samples. As the sample size decreases, the interpolation accuracy of OK and RBF methods is significantly affected, while the error of IDW-RBFNN remains relatively low. Considering both interpolation accuracy and resource limitations, we recommend using the IDW-RBFNN method (p = 2) with at least 60 samples as the minimum sampling density to ensure high interpolation accuracy under resource constraints. Our method overcomes limitations of existing approaches that use fixed steady-state distance decay parameters, providing an effective tool for soil fertility monitoring in delta regions.

Unraveling key environmental drivers of spatial variation in plant functional traits: Insights from Dacrydium pectinatum de Laub. in natural communities on Hainan Island, China

Accurate predictions of species distribution and coexistence in response to environmental changes depend on a thorough understanding of how functional traits relate to environmental conditions. However, there is still limited evidence regarding the sources of functional trait variation and the environmental mechanisms that regulate these traits in tropical forests. This study focuses on Dacrydium pectinatum de Laub., a crucial and endangered species native to the tropical mountain forests of Hainan Island, China. We gathered functional trait data from 68 permanent plots of D. pectinatum situated in three regions: Bawangling (163 tree species), Diaoluoshan (127 tree species), and Jianfengling (175 tree species). Nine functional traits were measured at the species level, and their variation patterns and sources were analyzed using linear mixed-effects models and variance decomposition methods. Our findings reveal that environmental pressures cause variations in trait relationships across different site conditions at the species level. Typically, inter-specific trait variation exceeds intra-specific variation, particularly for leaf area (LA) and specific leaf area (SLA). This indicates that trait variability is influenced by how species adapt to and balance specific environmental conditions. Site conditions significantly impact trait variation, especially for SLA, leaf dry matter content (LDMC), and wood density (WD). Additionally, intraspecific variation is notable for certain traits, reflecting individual responses to environmental heterogeneity. At the community level, eight out of nine traits exhibit significant changes in community-weighted mean (CWM) across various site conditions and environmental gradients. For instance, LA's CWM increases with soil fertility, whereas SLA, leaf nitrogen content (LNC), and leaf phosphorus content (LPC) show differences between site conditions. LDMC and WD decrease significantly in high-fertility environments, suggesting a transition from resource conservation to resource acquisition strategies within communities as soil fertility rises. Geographic variables play a crucial role in trait distributions across different community levels, with elevation and soil factors also contributing significantly to trait variation. Overall, our study provides direct evidence of how environmental filtering influences plant functional trait distributions and community assembly. Future research should explore the mechanisms driving these trait-environment relationships and their responses across various ecological contexts.

Applying Systems Thinking to Sustainable Beef Production Management: Modeling-Based Evidence for Enhancing Ecosystem Services

We used systems thinking (ST) to identify the critical components of beef cattle production through the lens of ecosystem services (ES), offering a holistic approach to address its adverse externalities. We identified eight critical feedback loops in beef production systems: (i) grazing and soil health, (ii) manure management and soil fertility, (iii) feed efficiency and meat production, (iv) water use and soil moisture, (v) cultural services and community engagement, (vi) energy use, (vii) carbon sequestration and climate regulation, and (viii) environmental impact. Our analysis reveals how these interconnected loops influence each other, demonstrating the complex nature of beef production systems. The dynamic hypothesis identified through the loops indicated that improved grazing and manure management practices enhance soil health, leading to better vegetation growth and cattle nutrition, which, in turn, have a positive impact on economic returns to producers and society, all of which encourage the continuation of interlinked beef and ecosystem stewardship practices. The management of beef production ES using ST might help cattle systems across the globe to contribute to 9 of the 17 different United Nations’ Sustainable Development Goals, including the “zero hunger” and “climate action” goals. We discussed the evaluation framework for agrifood systems developed by the economics of ecosystems and biodiversity to illustrate how ST in beef cattle systems could be harnessed to simultaneously achieve the intended environmental, economic, social, and health impacts of beef cattle systems. Our analysis of the literature for modeling and empirical case studies indicates that ST can reveal hidden feedback loops and interactions overlooked by traditional practices, leading to more sustainable beef cattle production outcomes. ST offers a robust framework for enhancing ES in beef cattle production by recognizing the interconnectedness of ecological and agricultural systems, enabling policymakers and managers to develop more effective and sustainable strategies that ensure the long-term health and resilience of humans and ES.

Long-term recovery of compacted reclaimed farmland soil in coal mining subsidence area

During the reclamation process of coal mining subsidence areas, mechanical compaction hinders the rapid recovery of reclaimed mine soil (RMS) functions to their pre-mining levels. To optimize reclaimed farmland management, which can promote the recovery of compacted soils, this study aims to explore the key factors and underlying mechanisms affecting RMS recovery from a systemic perspective using complex network theory (CNT). Soil samples from reclaimed farmland at different recovery stages (0, 2, 6, 12, 16, and 22 years) and adjacent non-subsided cultivated soils (NCS) were collected at a depth of 0 ∼ 20 cm in the eastern plains mining region of China. Twenty-four soil indicators were measured, and their evolution over RMS recovery was analyzed. CNT was employed to systematically analyze the complex network relationships among these indicators, identifying key factors and underlying mechanisms affecting RMS recovery. The results indicated that compaction led to soil macroaggregate (MA) destruction, mineralization losses of organic carbon and nitrogen, reduced microbial activity, degraded soil fertility, and increased complexity and disorder in the relationships among soil indicators. Re-cultivation had a positive effect on the recovery of RMS. After 22 years of cultivation, significant improvements in soil structure were observed, with MA increasing by 30.95 % (P < 0.05). Based on this, organic carbon gradually accumulated, with soil organic carbon (SOC) increasing by 250.94 % (P < 0.05). Microbial abundance similarly improved, with total microbial biomass (TB) increasing by 123.07 % (P < 0.05). Soil fertility was also enhanced, with alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), and available potassium (AK) increasing by 125.96 %, 304.84 %, and 61.90 %, respectively. However, RMS indicators and system structure remained distinct from those of the NCS. The first approximately 12 years after re-cultivation represented a critical period during which soil structure improvements drove RMS functional recovery, with a focus on MA recovery. Increasing particulate organic carbon (POC) accumulation is an effective strategy to promote aggregation in compacted RMS. Microbially mediated carbon–nitrogen cycling emerged as a crucial driver of the gradual recovery of the RMS system after re-cultivation.

Survey on Current Status, Challenges and Opportunities towards Integration of Traditional Smallholder Crop-livestock Systems in Kenya

The current study reports the results of a baseline survey conducted to establish current status of optimizing productivity of crop-livestock systems in Kenya. Results from the study showed that 74% and 18% of the household head and respondents were male and female respectively, indicating a male dominance of household headship. The household head (69%) and respondents (16%) reported to have completed classes for formal school. On average, farmers were walking 1.83 Km, 5.18 Km, 4.65 Km, and 12.79 Km to the nearest village market, town market, extension office and farmers’ training centers respectively. This shows that extension and communication services are still far apart from the farming communities, with a negative implication on technology adoption and household food and nutrition security. Majority of the farmers did not utilize organic nutrients for crop production (96%). Open heaping/piling, composting with other materials, use of solid and liquid manures was reported to be utilized on average 67 kg, 30 kg, 11 kg and 2 kg respectively. This implies a poor manure management, low adoption and utilization levels of organic manures, with subsequent impact on climate change, crop productivity and household food and nutrition security. The main constraint limiting the use of organic fertilizers were ranked as follows; ignorance of the technical aspects linked to the use of manure as an organic fertilizer (17.8%), low awareness of manure usefulness to improve soil fertility (16.4%), high affordability and timely accessibility of chemical fertilizers (16.3%). Results further show that majority of the respondent did not utilize Nitrogen fixing plants (66%), nutrient cycling (84%) and legume crops (75%). The main Nitrogen-fixing plants were faba bean (10%), Sesbania (9%), cowpea (5%), alfalfa (2%) and sunflower (1%) respectively. The survey recommends promotion of climate smart interventions and organic soil fertility management approaches, farmers capacity building and promotion of agribusiness.

Rice Under Dry Cultivation–Maize Intercropping Improves Soil Environment and Increases Total Yield by Regulating Belowground Root Growth

Under the one-season-a-year cropping pattern in Northeast China, continuous cropping is one of the main factors contributing to the degradation of black soil. Previous studies (on maize–soybean, maize–peanut, and maize–wheat intercropping) have shown that intercropping can alleviate this problem. However, it is not known whether intercropping is feasible for maize and rice under dry cultivation, and its effects on yield and soil fertility are unknown. A three-year field-orientation experiment was conducted at Jilin Agricultural University in Changchun city, Jilin Province, China, consisting of three cropping regimes, namely rice under dry cultivation–maize intercropping (IRM), sole rice under dry cultivation (SR), and sole maize (SM). All straw was fully returned to the field after mechanical harvesting. Rice under dry cultivation–maize intercropping with a land-equivalent ratio of 1.05 (the average of three years values) increased the total yield by 8.63% compared to the monoculture system. The aggressivity (A), relative crowding coefficient (K), time–area-equivalent ratio (ATER), and competition ratio (CR) value were positive or ≥1, also indicating that the rice under dry cultivation–maize intercropping had a yield advantage of the overall intercropping system. This is because the intercropped maize root length density (RLD) increased by 33.94–102.84% in the 0–40 cm soil layer, which contributed to an increase in the soil porosity (SP) of 5.58–10.10% in the 0–30 cm soil layer, an increase in the mean weight diameter of soil aggregates (MWD) of 3.00–15.69%, an increase in the geometric mean diameter of soil aggregates (GMD) of 8.16–26.42%, a decrease in the soil bulk density (SBD) of 4.02–7.35%, and an increase in the soil organic matter content (SOM) of 0.60–4.35%. This increased the water permeability and aeration of the soil and facilitated the absorption of nutrients and water by the root system and their transportation above ground, and the plant nitrogen, phosphorus, and potassium accumulation in the intercropping system were significantly higher than that in monoculture treatment, further promoting the total yield of intercropping. This suggests that rice under a dry cultivation–maize intercropping system is feasible in Northeast China, mainly because it promotes belowground root growth, improves the soil environment, and increases the total yield of intercropping.

Effect of Green Cowpea Manure on the Quality Properties of Sandy Soils Under the Specific Conditions of the Dry Climate in Romania

This study was carried out on the rotation of cowpea-rye + cowpea in successive crops for green manure-sorghum grains, with the objective of increasing the fertility of sandy soils by incorporating green cowpea manure into the soil in combination with chemical fertilization with NPK. In this sense, after harvesting the rye plant, the cowpea was sown, which was then incorporated into the soil during the formation of the beans in the pod. Before incorporating the cowpea biomass into the soil, the initial fertility status of the soil was assessed, and in the spring, the soil was sown with sorghum grain in two fertilization variants (N80P80K80 and N150P80K80). The soil chemical analyses carried out at the end of the vegetation cycle of the sorghum plant showed that by incorporating 52.4 t/ha green cowpea biomass (9.24 t/ha dry biomass), the quality properties of the soil were significantly improved, with annual increases of 0.17–0.29 g/kg in total nitrogen, 9.5–13.33 mg/kg in extractable phosphorus, 11.33–24 mg/kg in exchangeable potassium, and 0.08–0.12 g/kg in organic carbon, also depending on the sorghum fertilization system. It has also been shown to reduce the acidity of the soil pH and improve the water holding capacity of the soil.

Precision Agriculture using ML for Soil and Weather Prediction

Agriculture is the backbone of many economies and plays a critical role in global food security. However, with growing challenges posed by climate change, erratic weather patterns, and soil degradation, the need for precise and predictive techniques in agriculture has never been more urgent. This paper focuses on the application of machine learning (ML) techniques in soil fertility prediction and weather forecasting, two critical components that can significantly impact agricultural productivity. By analysing soil properties, such as moisture, pH, and nutrient levels, and combining them with accurate weather predictions, our system aims to help farmers make informed decisions regarding crop selection, irrigation scheduling, and pest control. Leveraging algorithms like Naive Bayes for soil classification and Long Short-Term Memory (LSTM) networks for weather prediction, we provide a comprehensive solution for precision agriculture. This system not only enhances productivity but also promotes sustainable agricultural practices by optimizing resource use and reducing wastage

Biofertilizers: A sustainable strategy for enhancing physical, chemical, and biological properties of soil

A biofertilizer is a biologically derived substance that can enhance soil fertility. It is beneficial for enhancing soil fertility by introducing microorganisms that produce organic nutrients and perhaps decreasing plant illnesses. An extensive review was done to examine the efficacy of several biofertilizers in improving soil properties, including their physical, chemical, and biological characteristics. The secondary data and material for the article were gathered from a variety of sources, including government reports, published research papers, reports from various organizations, and pertinent websites that were examined and their conclusions presented. Although biofertilizers have proven to be a very sustainable method for improving soil quality and increasing crop output, there is a lack of extensive research on their effects on soil’s physical, chemical, and biological aspects. This study aims to offer a comprehensive understanding of various biofertilizers and their effects. This investigation indicated that biofertilizers such as Nitrogen-fixing bacteria, phosphate solubilizers, and potassium solubilizers help to increase NPK content in the soil leading to an increase in the productivity of soil. Moreover, biofertilizers help to enhance soil physical properties (soil bulk density, soil moisture, soil temperature, and soil color), chemical properties (soil pH, soil nitrogen content, soil phosphorus content, soil potassium content, and soil organic carbon content), and chemical properties (population of bacteria, fungi, and actinomycetes). Overall, by overcoming the challenges with biofertilizers in agriculture, we can attain agricultural sustainability.

The effects of livestock grazing on physicochemical properties and bacterial communities of perlite-rich soil.

Livestock grazing has been proposed as a cost-effective way to reclaim post-mining lands. It can enhance soil fertility and biodiversity, but its impacts on soil quality and microbial communities vary across soil types. Moreover, waste from grazing raises concerns about pathogens that could pose risks to animal and human health. This study investigated the effects of grazing on post-mining perlite-rich soil in central Thailand. A comparative analysis of soil physicochemical properties and bacterial diversity was conducted between grazed and ungrazed sites. Bacterial diversity was assessed using 16S amplicon sequencing. The perlite-rich soil was found to be sandy, acidic, and to have low nutritional content. Grazing significantly improved the soil texture and nutrient content, suggesting its potential as a cost-effective reclamation strategy. The 16S metagenomic sequencing analysis revealed that microbial communities were impacted by livestock grazing. Specifically, shifts in the dominant bacterial phyla were identified, with increases in Firmicutes and Chloroflexi and a decrease in Actinobacteria. Concerns about increased levels of pathogenic Enterobacteriaceae due to grazing were not substantiated in perlite-rich soil. These bacteria were consistently found at low levels in all soil samples, regardless of livestock grazing. This study also identified a diverse population of Streptomycetaceae, including previously uncharacterized strains/species. This finding could be valuable given that this bacterial family is known for producing antibiotics and other secondary metabolites. However, grazing adversely impacted the abundance and diversity of Streptomycetaceae in this specific soil type. In line with previous research, this study demonstrated that the response of soil microbial communities to grazing varies significantly depending on the soil type, with unique responses appearing to be associated with perlite-rich soil. This emphasizes the importance of soil-specific research in understanding how grazing affects microbial communities. Future research should focus on optimizing grazing practices for perlite-rich soil and characterizing the Streptomycetaceae community for potential antibiotic and secondary metabolite discovery. The obtained findings should ultimately contribute to sustainable post-mining reclamation through livestock grazing and the preservation of valuable microbial resources.

Soil nitrogen-related functional genes undergo abundance changes during vegetation degradation in a Qinghai-Tibet Plateau wet meadow.

Our research investigates how the degradation of meadows affects the tiny organisms in soil that help plants use nitrogen, which is essential for their growth. In the Qinghai-Tibet Plateau, a region known for its unique ecosystems, we found that as meadows deteriorate-due to climate change and human activities-the number of these beneficial organisms significantly decreases. This decline could reduce soil fertility, impacting plant life and the overall health of the ecosystem. Understanding these changes helps us grasp how environmental pressures influence soil and plant health. Such knowledge is crucial for developing strategies to preserve these vulnerable ecosystems and ensure they continue to sustain biodiversity and provide resources for local communities.

Transitioning practices of vegetable small-scale actors in Vietnam: an interplay of food safety, labor demand, and soil environment

AbstractFood safety is a critical and persistent issue that challenges the sustainability of agri-food systems in Vietnam. The government has launched multiple food safety initiatives, but there is limited understanding of their contribution to changing the practices of small-scale producers and distributors. This study explores these changing practices by applying Social Practice Theory (SPT) to analyze the transitions in everyday routines of small-scale vegetable producers while being embedded in socio-institutional contexts of agri-food system transitions. We conducted semi-structured interviews and survey with small-scale food producers and distributors in Hanoi, Vietnam to examine the transitions in production and post-production practices over the last 20 years and the intersection between smallholding practices and cross-level dynamics. The study revealed, contrary to some common perceptions, that smallholder producers are transitioning towards food safety, with the use of more bio-pesticides and eco-friendly pest control methods. The smallholders also reproduce a variety of (sustainable) intensification practices, including crop rotation, organic fertilization, and soil cultivation, to sustain soil fertility and save labor. However, there are no clear patterns of change for post-production practices, although they have been diversifying under the impacts of urbanization. The findings highlight the interplay of food safety, labor, and soil environment in shaping the transitions of smallholder practices. We suggest that success in improving safety in production practices is feasible, but that this requires more thorough interventions in distribution and consumption practices to transform the food systems at large.

Improving maize yield and drought tolerance in field conditions through activated biochar application

Amidst depleting water resources, rising crop water needs, changing climates, and soil fertility decline from inorganic modifications of soil, the need for sustainable agricultural solutions has been more pressing. The experimental work aimed to inspect the potential of organically activated biochar in improving soil physicochemical and nutrient status as well as improving biochemical and physiological processes, and optimizing yield-related attributes under optimal and deficit irrigation conditions. Biochar enhances soil structure, water retention, and nutrient availability, while improving plant nutrient uptake and drought resilience. The field experiment with maize crop was conducted in Hardaas Pur (32°38.37’N, 74°9.00’E), Gujrat, Pakistan. The experiment involved the use of DK-9108, DK-6321, and Sarhaab maize hybrid seeds, with five moisture levels of evapotranspiration (100% ETC, 80% ETC, 70% ETC, 60% ETC, and 50% ETC) maintained throughout the crop seasons. Furthermore, activated biochar was applied at three levels: 0 tons/ha (no biochar), 5 tons per hectare, and 10 tons per hectare. The study’s findings revealed significant improvements in soil organic matter, bulk density, nutrient profile and total porosity with biochar supplementation in soil. Maize plants grown under lower levels of ETC in biochar supplemented soil had enhanced membrane stability index (1.6 times higher) increased protein content (1.4 times higher), reduced malondialdehyde levels (0.7 times lower), improved antioxidant enzyme activity (1.3 times more SOD and POD activity, and 1.2 times more CAT activity), improved relative growth (1.05 times more) and enhanced yield parameters (26% more grain and stover yield, 16% more 1000-seed weight, 29% more total seed weight, 33% more apparent water productivity) than control. Additionally, among the two biochar application levels tested, the 5 tons/ha dose demonstrated superior efficiency compared to the 10 tons/ha biochar dose.