Soil Faunal Activity Research Articles

Impacts of Climate change on soil microbial diversity, distribution and abundance

Climate change, driven by anthropogenic activities, has far-reaching consequences for our planet. Among its many impacts, changes in temperature, elevated carbon dioxide levels, and shifts in greenhouse gas concentrations significantly affect soil ecosystems. In particular, soil microbial communities play a pivotal role in nutrient cycling, organic matter decomposition, and overall soil health. Soil microbial communities respond differently to the effects of climate change, like elevated warming and precipitation. The change in climatic conditions is reported to be adversely affecting soil biological activity directly through either drying or wetting of soil or affecting their associated plants. This review delves into the intricate relationship between climate change and soil microbial abundance, diversity, and distribution. The paper also discusses climatic change pressure on soil enzymatic activity and microbial biomasses, as well as soil faunal activity, as they are key indicators of soil health in a changing climate. Soil microbial communities cope with climate change by changing their diversity and physiological characteristics and by changing their symbiotic plants, which indicates the role of soil microbes in withstanding the negative impact of climate change.

Effects of ant nests on soil CH4 emissions from Syzygium oblatum communities of a secondary tropical forest.

Exploring the effects of ant nests on soil CH4 emissions in the secondary tropical forests is of great scientific significance to understand the contribution of soil faunal activities to greenhouse gas emissions. With static chamber-gas chromatography method, we measured the dry-wet seasonal dynamics of CH4 emissions from ant nests and control soils in the secondary forest of Syzygium oblatum communities in Xishuangbanna. We also examined the linkages of ant-mediated changes in functional microbial diversity and soil physicochemical properties with CH4 emissions. The results showed that: 1) Ant nests significantly accelerated soil CH4 emissions, with average CH4 emissions in the ant nests being 2.6-fold of that in the control soils. 2) The CH4 emissions had significant dry-wet seasonal variations, which was a carbon sink in the dry seasons (from -0.29±0.03 to -0.53±0.02 μg·m-2·h-1) and a carbon source in the wet seasons (from 0.098±0.02 to 0.041±0.009 μg·m-2·h-1). The CH4 emissions were significantly higher in ant nests than in control soils. The CH4 emissions from the ant nests had smaller dry-wet seasonal variation (from -0.38±0.01 to 0.12±0.02 μg·m-2·h-1) than those in the control soils (from -0.65±0.04 to 0.058±0.006 μg·m-2·h-1). 3) Ant nests significantly increased the values (6.2%-37.8%) of soil methanogen diversity (i.e., Ace and Shannon indices), temperature and humidity, carbon pools (i.e., total, easily oxidizable, and microbial carbon), and nitrogen pools (i.e., total, hydrolyzed, ammonium, and microbial biomass nitrogen), but decreased the diversity (i.e., Ace and Chao1 indices) of methane-oxidizing bacteria by 21.9%-23.8%. 4) Results of the structural equation modeling showed that CH4 emissions were promoted by soil methanogen diversity, temperature and humidity, and C and N pools, but inhibited by soil methane-oxidizing bacterial diversity. The explained extents of soil temperature, humidity, carbon pool, nitrogen pool, methanogen diversity, and methane-oxidizing bacterial diversity for the CH4 emission changes were 6.9%, 21.6%, 18.4%, 15.2%, 14.0%, and 10.8%, respectively. Therefore, ant nests regulated soil CH4 emission dynamics through altering soil functional bacterial diversities, micro-habitat, and carbon and nitrogen pools in the secondary tropical forests.

Influence of organic fertilization on growth and yield of strawberry (Fragaria × ananassa) in Kabete and Mbooni areas, Kenya

Strawberries are a valuable crop in Kenya with the potential for significant economic contributions. However, strawberry production in the country has been facing considerable challenges, impacting its economic potential. This study examined the influence of organic manure on strawberry growth and characteristics in Kabete and Mbooni areas in Kenya. The study used a randomized complete block design (RCBD) with three replications. Treatments included livestock manure (well composted mixture of chicken, goat, and cow manure), bokashi manure, and a control, coded as LivManure, BokManure and Control, respectively. Growth parameters including leaf area, number of white flowers and number of runners, as well as yield parameters such as the number and weight of strawberries were assessed from the 3rd to 10th week after transplanting, during the short rain season of 2021. Using R statistical software, linear models were fitted to datasets from both study sites and analyzed using one-way ANOVA, followed by post-hoc tests for multiple comparisons. The rigorous analysis of the Kabete and Mbooni datasets provided insightful revelations about the influence of different treatments on strawberry characteristics, and geographical disparities between the two regions. The analysis of variance (ANOVA) outcomes unveiled significant treatment effects in both sites, with F(2,69) = 62.57, p < 0.001 for Kabete and F(2,69) = 49.02, p < 0.001 for Mbooni, highlighting distinct influences of treatments on log values within each group. Post hoc analyses, including Tukey tests and bootstrap comparisons robustly validated the significant differences among the three treatments in each site, supported by p-values <0.001. Effect sizes were also employed to reinforce the findings, and planned contrasts were set to gain more power in the analysis of variance. Comparison between Kabete and Mbooni indicated a significant difference of 9.78 units, with Mbooni area exhibiting significantly higher strawberry characteristics compared to Kabete. The results showed that LivManure treatment had the highest mean in both sites, followed by BokManure and Control treatments, respectively. These findings have important implications for agriculture, and highlight the potential benefits of using LivManure treatment to improve strawberry characteristics in similar agroclimatic settings. These observations can be attributed to the beneficial effects of livestock manure on soil health, which include buffering of the soil reaction, provision of essential plant nutrients and enhancement of soil faunal activities. Balanced use of livestock manure is recommended to enhance soil macro and micronutrients, and soil reaction for improved growth and yield of strawberry.

Liming leads to changes in the physical properties of acidified forest soils

AbstractBackgroundForest liming is a common practice in many German forests, which aims primarily at improving soil chemical properties that have been negatively impacted by acid rain. Effects on physical functions have not yet been studied widely.AimsThe hypothesis of this study is that chemical changes after liming lead to changes in the physical properties of the humus layer and mineral soil.MethodsWe studied soil physical characteristics (water retention characteristics, air and water permeabilities, and conductivities) of limed and adjacent control plots of sandy to loamy, acidified soils under spruce and beech‐dominated stands.ResultsWe found differences between limed and control plots, especially in the humus layer and mineral top soil. Here, air capacity values (representing air‐filled macropores at −6 kPa) have experienced an increase, while available water capacities (representing the sum of dewatered mesopores between −6 and −1500 kPa) were reduced to a variable extent. These changes in pore size distributions affected gas diffusion as well as gas permeability positively. Unsaturated hydraulic conductivity was not affected. Below the top mineral soil, a tendency to a reduced macroporosity and gas permeability was observed, but no clear changes were detected.ConclusionsSmall but significant changes of soil physical properties and functions in the humus layer and top mineral soil are discussed to be an indirect effect of forest liming. Liming improves pH and nutrient availability, which in turn should have stimulated activity and abundance of soil fauna, especially earthworms. Where observed, their activity could explain the observed changes in soil physical properties. We believe that effects below the top soil might be due to mobilization and translocation of soil particles and a clogging of pores, but these depths were only studied at three plots. Compared to common liming practices, however, the investigated sites are characterized by significantly higher lime applications. The effects on practice liming areas in forestry praxis are therefore likely to be smaller than in this study.

Seasonal Dynamics of Soil Respiration and Its Autotrophic and Heterotrophic Components in Subtropical Camphor Forests

On a global scale, soil respiration (Rs), representing the CO2 flux between the soil surface and the atmosphere, ranks as the second-largest terrestrial carbon (C) flux. Understanding the dynamics between Rs and its autotrophic (Ra) and heterotrophic (Rh) components is necessary for accurately evaluating and predicting global C balance and net ecosystem production under environmental change. In this study, we conducted a two-year root exclusion experiment in subtropical China’s Camphor (Cinnamomum camphora (L.) Presl.) forests to assess seasonal changes in Ra and Rh and their relative contributions to Rs. Additionally, we examined the influence of environmental factors on the dynamics of Ra, Rh, and Rs. Our results showed that seasonal mean Rs values were 2.88 µmol m−2 s−1, with mean Ra and Rh of 1.21 and 1.67 µmol m−2 s−1, respectively, in the studied forests. On an annual basis, the annual values of mean Rs in the studied forests were 405 ± 219 g C m−2 year−1, with Rh and Ra accounting for 240 ± 120 and 164 ± 102 g C m−2 year−1, respectively. The seasonal mean ratio of Rh to Rs (Rh/Rs) was 58%, varying from 45 to 81%. Seasonal changes in Rs and Rh were strongly correlated with soil temperature but not soil water content. Both Rh and Rs increased exponentially with the average soil temperature measured in the topsoil layer (about 5 cm), with Q10 values of 2.02 and 1.73 for Rh and Rs, respectively. Our results suggest that the composition and activity of soil microbes and fauna play a primary role in releasing carbon flux from soil to the atmosphere in the studied forest ecosystems.

Livestock grazing modifies soil nematode body size structure in mosaic grassland habitats

Body size is closely related to the trophic level and abundance of soil fauna, particularly nematodes. Therefore, size-based analyses are increasingly prominent in unveiling soil food web structure and its responses to anthropogenic disturbances, such as livestock grazing. Yet, little is known about the effects of different livestock on the body size structure of soil nematodes, especially in grasslands characterized by local habitat heterogeneity. A four-year field grazing experiment from 2017 to 2020 was conducted in a meadow steppe characterized by typical mosaics of degraded hypersaline patches and undegraded hyposaline patches to assess the impacts of cattle and sheep grazing on the body size structure of soil nematodes within and across trophic groups. Without grazing, the hypersaline patches harbored higher abundance of large-bodied nematodes in the community compared to the hyposaline patches. Livestock grazing decreased large-bodied nematodes within and across trophic groups mainly by reducing soil microbial biomass in the hypersaline patches, with sheep grazing resulting in more substantial reductions compared to cattle grazing. The reduction in large-bodied nematode individuals correspondingly resulted in decreases in nematode community-weighted mean (CWM) body size, nematode biomass, and size spectra slopes. However, both cattle and sheep grazing had minimal impacts on the CWM body size and size spectra of total nematodes in the hyposaline patches. Our findings suggest that livestock grazing, especially sheep grazing, has the potential to simplify soil food webs by reducing large-bodied nematodes in degraded habitats, which may aggravate soil degradation by weakening the bioturbation activities of soil fauna. In light of the widespread land use of grasslands by herbivores of various species and the ongoing global grassland degradation of mosaic patches, the recognition of the trends revealed by our findings is critical for developing appropriate strategies for grassland grazing management.

Hydrological regime and forest development have indirect effects on soil fauna feeding activity in Central European hardwood floodplain forests

Soil fauna act as regulators of decomposition processes via their feeding activity, thereby playing an important role in regulating carbon cycling and sequestration. Hardwood floodplain forests are critically endangered habitats, but strongly contribute to carbon sequestration in Central Europe. In the present study, within a floodplain forest-development programme, we investigated the feeding activity of soil fauna via the Bait Lamina test in hardwood floodplain forests of the middle Elbe River in Germany in sites with different hydrological regimes and forest-development stages, with neighbouring grassland sites for comparison. While statistically significant differences in overall feeding activity between general hydrological regimes or forest development stages were not found, decreases in feeding activity with soil depth were strongly modulated by these factors, indicating more unfavourable conditions for soil fauna at increasing soil depth due to, e.g., anoxic conditions in floodplains of tributaries or low soil moisture content below the shallow rooting zone of grasslands. Registered effects of soil texture on soil fauna feeding activity were dependent on forest-tree density, and combined effects indicate that soil-fauna feeding activity varies with soil temperature during spring, but with soil moisture in early autumn. In conclusion, our results highlight the importance of the current abiotic conditions on soil-fauna feeding activities in floodplain forests, i.e. soil temperature, moisture and ground water level. Hydrological regime and forest development have a strong impact on the effect of these conditions, indirectly affecting soil fauna feeding activity and highlighting the multifactorial influence on soil fauna functional activity to be considered in floodplain-forest restoration programs.

Long-term activity of social insects responsible for the physical fertility of soils in the tropics

Ferralsols correspond to the red and yellow soils that are common in the tropics. They are deeply weathered but physical fertility is high because they exhibit a strong microgranular structure whose origin is still actively debated. In the present study, we looked for evidence of the biological origin of the structure resulting from soil fauna activity. We present results recorded with Brazilian Ferralsols developed under native vegetation. It was found that the Ferralsols studied exhibit morphological features related to the activity of social insects. We showed the presence of potassium 2:1 clays originating from the saprolite in the microaggregates of all the Ferralsols studied. These 2:1 clays were earlier discussed as markers of long-term termite activity. This highlights the threat that weighs on the physical fertility of these soils, and more broadly on the water cycle in the tropical regions concerned, if intensive agriculture reduces the soil fauna biodiversity, as indicated by several studies.

Using Dye and Bromide Tracers to Identify Preferential Water Flow in Agricultural Hillslope Soil under Controlled Conditions

Processes in hillslope soils present a particular challenge for agricultural production and soil management due to their hydropedological specifics and high soil erosion risk. Soil heterogeneities can cause preferential and/or lateral flow on the entire hillslope resulting in the off-site movement of water, fertilizers and chemicals used in crop production. A study was conducted under controlled conditions in a laboratory with undisturbed soil cores (250 cm3), which were used to estimate the soil hydraulic properties (SHP) using HYPROP and WP4C devices, while undisturbed soil columns (diameter = 16 cm, length = 25 cm) were used for the evaluation of preferential flow pathways using potassium bromide and Brilliant Blue. Samples were excavated in triplicate from the hilltop, backslope and footslope regions within the inter-rows of a vineyard from a critical zone observatory, SUPREHILL, in Croatia in Dystric Luvic Stagnosol. The aim of this study was to determine if the erosion-affected hillslope position affected the physical, chemical and hydraulic properties of soil and to identify water flow and possible preferential flow using dye and bromide tracers. The results of the sensor measurements and estimated SHPs were in agreement, showing a faster leaching of the irrigated rainwater in the footslope column. The tracer experiments showed variability even in the columns taken from the same position on the hillslope, which can be linked to plant roots and soil fauna activity. Altogether, the results showed a deeper loose layer at the footslope as a consequence of the soil erosion, which then resulted in higher hydraulic conductivity and the leached mass of the bromide due to better soil structure and pore connectivity. Thus, due to significant differences in the leached mass of bromide, this research should be later expanded in field experiments to reveal the impact of surface runoff, subsurface preferential and lateral flow on a larger scale.

Fired shards from selected ancient Anatolian ceramics: a brief review of their mineralogical nature and pedological?microstructural evolution

This review is concerned with some key features observed within grouped samples of ancient ceramics that provide important evidence concerning the selection of appropriate raw materials and the evolution of the firing technologies employed in their production. It also demonstrates the importance of distinguishing and accounting for the mineralogical and microstructural attributes and identifying the processes responsible for the microstructural evolution of these ancient ceramics. The mineralogy largely reflects both the nature of the raw materials used and the maximum temperatures achieved during firing, deduced from the presence of specific high temperature minerals (HTMs). The microstructural evolution processes, deduced by the micromorphology of these ancient ceramics and displayed in specific features, observed in both the matrix and the slip of these ceramics, are largely controlled by the firing methods used in manufacturing. Thus, we conclude that the application of micromorphological principles, methods, and observations derived from the broad sphere of ?pedology? to the study of ancient ceramics, provides valuable insights into the independent evolution of ceramic production methods in ancient societies. Thus, most of the observations recorded here concern identification of the raw materials used to make ancient ceramics and the firing processes used in their manufacture. Our data demonstrate that these ancient potters made use of a variety of temper materials (quartz and chaff, together with fragments of locally available rocks and minerals) that are now preserved in the matrix. Furthermore, analyses of the micromorphological attributes displayed by these ancient ceramics are helpful in determining and explaining the shrinkage features (stress coatings or poro-striated b-fabrics) and the preferred orientation of the elongated pores that have developed after firing in poorly controlled and slow-fired furnaces. In this regard, we finally seek to develop a useful data library ultimately targeting the enhancement of simulated ancient ceramic/pottery production, with an overall objective to apply the mineralogical and pedological properties of Anatolian ceramics researched in this work to globally selected shard specimens. Postburial processes, such as the illuviation-deposition of clay minerals to form the observed clay coatings, probably operated during the wet-dry cycles associated with mid-late Holocene climatic fluctuations. Accompanying calcification-decalcification processes, which may result from an intra cramic leaching-deposition of carbonate present in the source material of the pottery, are also consistent with the known wet-dry cycles of the mid to late Holocene pedogenesis (soil formation) episode and is reflected in the clay coatings and further attested by coeval changes in the soil-faunal activity.

Diversity and activity of soil biota at a post-mining site highly contaminated with Zn and Cd are enhanced by metallicolous compared to non-metallicolous Arabidopsis halleri ecotypes.

Hyperaccumulators' ability to take up large quantities of harmful heavy metals from contaminated soils and store them in their foliage makes them promising organisms for bioremediation. Here we demonstrate that some ecotypes of the zinc hyperaccumulator Arabidopsis halleri are more suitable for bioremediation than others, because of their distinct influence on soil biota. In a field experiment, populations originating from metal-polluted and unpolluted soils were transplanted to a highly contaminated metalliferous site in Southern Poland. Effects of plant ecotypes on soil biota were assessed by measurements of feeding activity of soil fauna (bait-lamina test) and catabolic activity and functional diversity of soil bacteria underneath A. halleri plants (Biolog® ECO plates). Chemical soil properties, plant morphological parameters, and zinc concentration in shoots and roots were additionally evaluated. Higher soil fauna feeding activity and higher bacterial community functional diversity were found in soils affected by A. halleri plants originating from metallicolous compared to non-metallicolous ecotypes. Differences in community-level physiological profiles further evidenced changes in microbial communities in response to plant ecotype. These soil characteristics were positively correlated with plant size. No differences in zinc content in shoots and roots, zinc translocation ratio, and plant morphology were observed between metallicolous and non-metallicolous plants. Our results indicate strong associations between A. halleri ecotype and soil microbial community properties. In particular, the improvement of soil biological properties by metallicolous accessions should be further explored to optimize hyperaccumulator-based bioremediation technologies.

Mycorrhiza-mediated nitrogen cycling depends on earthworm behavior under different straw management regimes

Arbuscular mycorrhizal fungi (AMF) play important roles in some key steps of nitrogen cycling in agroecosystems, such as the transformation of soil nitrogen and plant nitrogen uptake. However, whether AMF-mediated nitrogen cycling can be affected by other soil organisms, such as earthworms, remains to be elucidated, particularly under straw incorporation. A factorial microcosm experiment was conducted to test the effects of AMF-earthworm interactions on nitrogen cycling by including two earthworm species with different feeding behaviors (Eisenia fetida, epigeic, EP and Metaphire guillelmi, endogeic, EN) under different straw management strategies (no straw, NS; straw mixing with soil in 0–10 cm depth, SM; and the deep burial of straw at 20 cm deep, DB). Our results showed that AMF significantly increased the aboveground plant nitrogen uptake under NS (+27.01 %), SM (+7.63 %) and DB (+14.42 %) treatments. However, interactions between earthworms with different feeding types and straw management could affect AMF-mediated nitrification and denitrification in soils. In addition, earthworms were found to promote the uptake of mycorrhizal 15N uptake in the SM treatment but reduced it under the DB treatment. However, the interaction between AMF and EN earthworms promoted the total plant N uptake in the DB treatment. Our study suggests that AMF can affect the transformation of soil nitrogen and plant nitrogen uptake, but their impacts could be altered by agronomic management, such as straw incorporation, which induced changes in the activities of soil fauna, such as earthworms.

Let's make a mess, maybe no one will notice. The impact of bioturbation activity on the urn fill condition.

The research was carried out at the cremation cemetery of the Lusatian culture in Wtórek, Ostrów Wielkopolski district, Wielkopolska province, Poland. Contrary to the so-far-studied topics related to the CT imaging of burnt bones and their virtual exploration, we concentrated on the analysis of the structures formed by the soil fauna activity in the fills of urns and additional vessels, and reconstruction of the dynamics of the ecosystem variability within the cemetery area based on thereof. We also demonstrated the impact of macrofaunal activity on stratigraphy and bone fragmentation. From the total of 222 excavated burials in 18 urns and one additional vessel, the remains of macrofauna or its bioturbation activity were identified. Out of 19 vessels subjected to CT examinations, traces of macrofaunal activity were demonstrated in 13: in five vessels animal bioturbative activity was not observed and in one, observations was impossible (due to significant metal-related artifacts). In two vessels both macrofaunal remains and traces of activity were identified. Discovered bioturbations were associated with specific species or genera. Nests or their parts of the genus Geotrupes sp. beetles were the most frequently observed traces of macrofaunal activity. Tunnels and aestivation chambers of earthworms and chambers of the genus Harpalus sp. beetles filled with Setaria sp. caryopses were discovered. The chitinous parts of other insects and the humerus bones of the vole of the genus Microtus sp. were also identified. It was shown, especially due to the non-destructive method, that rodents activity had the most destructive effect on the bone stratigraphy as well as on the movement and fragmentation of the burnt bones. The chances of visualizing bioturbations decreased with time since their creation. The process of disappearance of traces of macrofaunal activity concerned both traces of rodent activity and nests set up by Geotrupes sp. and other species.

Cover crop influence on pore size distribution and biopore dynamics: Enumerating root and soil faunal effects.

Pore structure is a key determinant of soil functioning, and both root growth and activity of soil fauna are modified by and interact with pore structure in multiple ways. Cover cropping is a rapidly growing popular strategy for improving agricultural sustainability, including improvements in pore structure. However, since cover crop species encompass a variety of contrasting root architectures, they can have disparate effects on formation of soil pores and their characteristics, thus on the pore structure formation. Moreover, utilization of the existing pore systems and its modification by new root growth, in conjunction with soil fauna activity, can also vary by cover crop species, affecting the dynamics of biopores (creation and demolition). The objectives of this study were (i) to quantify the influence of 5 cover crop species on formation and size distribution of soil macropores (>36 μm Ø); (ii) to explore the changes in the originally developed pore architecture after an additional season of cover crop growth; and (iii) to assess the relative contributions of plant roots and soil fauna to fate and modifications of biopores. Intact soil cores were taken from 5 to 10 cm depth after one season of cover crop growth, followed by X-ray computed micro-tomography (CT) characterization, and then, the cores were reburied for a second root growing period of cover crops to explore subsequent changes in pore characteristics with the second CT scanning.Our data suggest that interactions of soil fauna and roots with pore structure changed over time. While in the first season, large biopores were created at the expense of small pores, in the second year these biopores were reused or destroyed by the creation of new ones through earthworm activities and large root growth. In addition, the creation of large biopores (>0.5 mm) increased total macroporosity. During the second root growing period, these large sized macropores, however, are reduced in size again through the action of soil fauna smaller than earthworms, suggesting a highly dynamic equilibrium. Different effects of cover crops on pore structure mainly arise from their differences in root volume, mean diameter as well as their reuse of existing macropores.

Variations in Litterfall Dynamics, C:N:P Stoichiometry and Associated Nutrient Return in Pure and Mixed Stands of Camphor Tree and Masson Pine Forests

Litterfall, directly and indirectly, affects the soil physicochemical properties, microbial activity, and diversity of soil fauna and flora by adding organic matter and nutrients to the soil. This study explores litterfall dynamics such as litterfall production, litter decomposition rate, and associated nutrient return in three forest types, that is, camphor tree forest (CTF), Masson pine forest (MPF), and camphor tree and Masson pine mixed forest (CMF), in subtropical China. Results showed that CMF had the highest mean annual litterfall production (4.30 ± 0.22 t ha−1), which was significantly higher than that of MPF (3.41 ± 0.25 t ha−1) and CTF (3.26 ± 0.17 t ha−1). Leaf represented the major fraction of litterfall, constituting over 71% of the total litterfall mass in the three forest types. The contribution of branch litter was 16.3, 8.9, and 16.9%, and miscellaneous litter was 12.6, 18.9, and 11.1% in CTF, MPF, and CMF, respectively. The concentration of macronutrients ranked as N &amp;gt; Ca &amp;gt; K &amp;gt; Mg &amp;gt; P in all litter fractions. The total annual macronutrient return to the soil from the litterfall was in order as CTF (74.2 kg ha−1‧yr−1) &amp;gt; CMF (70.7 kg ha−1‧yr−1) &amp;gt; MPF (33.6 kg ha−1‧yr−1). The decomposition rate was higher in leaf litter than in branch litter throughout the three forests. Among the forest types, the leaf and branch decomposition rates were in a pattern: CTF &amp;gt; CMF &amp;gt; MPF. The ratio of C/N in both leaf and branch litters was significantly higher in MPF than in CTF and CMF, while no significant differences in N/P ratio were found in these litters among the three forests. The high N:P ratios in leaf litter (23/30) and the branch (24/32) litter indicated the high N returning and low nutrient returning to the soil. Our results suggested that the broadleaved forests have faster litter decomposition and higher macronutrient returns than conifer forests. Moreover, the litter decomposition rate was mainly associated with litterfall quality and chemical composition. The introduction of broadleaved trees into monoculture coniferous stands could increase litter production nutrients return, and thus, it had advantages in soil nutrients restoration and sustainable forest management.

Mixed Forest of Larix principis-rupprechtii and Betula platyphylla Modulating Soil Fauna Diversity and Improving Faunal Effect on Litter Decomposition

This research performed a comparison study to investigate how mixed forest affects the abundance, groups, and diversity of soil fauna and the effects of soil fauna on litter decomposition. We comparatively studied two forests, Larix principis-rupprechtii forest (LF) and mixed Larix principis-rupprechtii and Betula platyphylla forest (MF), which hold 30 years of stand age and are the representative forests in the mountainous area of northwestern Hebei, China. The field experiments were conducted from May to November 2020, with soil fauna and litter samples taken every one and a half months. A total of 540 soil samples (replicated samples, 3) were collected in each forest and the soil faunas were extracted from the samples by Tullgren methods in laboratory. Litter samples were incubated separately in the sampled forests using litterbags with two mesh sizes (0.01 and 4 mm) to observe the decomposition rate. In total, 2958 (inds.) soil faunas belonging to 4 phyla, 11 classes, and 20 orders, were found, with Acarina (1079/2958; 36.48%) and Collembola (1080/2958; 36.51%) being the dominant groups. The total abundance of soil fauna in the MF (1581 inds.) was higher than that in the LF (1377 inds.), and the significantly more abundant predatory functional group in the MF (p &lt; 0.05) may indicate a more complex soil fauna food web structure. Comparatively, the higher Shannon–Wiener index (1.42–1.74) and Pielou evenness index (0.58–0.71) and the lower Simpson dominance index (0.22–0.32) in the MF suggested that the MF promoted the soil fauna diversity. The cumulative litter decomposition rate of litterbags with 4 mm aperture in the MF (54.52% in 300 days) was higher than that in the LF (32.81% in 300 days). Moreover, the litter decomposition rate was positively correlated with the total abundance and the number of groups, and was negatively with the Simpson dominance index, implying that the soil fauna activity effectively improved litter decomposition in the MF. Via the comparison, we found that the mixture of plant species in the forest can modulate the soil fauna diversity and accelerate the litter decomposition. The results in this study may provide an interesting reference for forest restoration and sustainable management.

Micro- within macro: How micro-aggregation shapes the soil pore space and water-stability

Soil microaggregates are characterized by higher stability than macroaggregates; at the same time, they are their building units. The water-stability of soil structure is generally defined by architecture parameters, strength of linkages between the solids and is usually assessed by the size of the structural units into which the aggregates break down. Therefore, we hypothesized that within the shift of capillary to full saturation, macroaggregate water-stability is determined primarily by the content and the size of microaggregates, and number of contacts between the building units within macroaggregates.To test this hypothesis, two soil aggregate fractions (10–7 and 5–3 mm) were separated by the dry sieving from A horizons of the boreal forest and arable loamy Retisols contrasting in types of macroaggregates. Aggregate architecture was depicted by X-ray computed microtomography (µCT) with resolution 6.97 µm (15 aggregates of each fraction) and than the water-stability test of the individual aggregates with the subsequent analysis of fragment size distributions into which the aggregates had disintegrated by laser diffractometry was performed.Microaggregates are the prerequisites to the formation of complex and openwork aggregate pore space. For the studied soils, when the number of microaggregates is above 55%, a further increase of every one percent leads to 10 new contacts between the solids in the macroaggregates. A higher degree of intra-macroaggregate porosity is achieved with an increased number of micropores enclosed within the microaggregates and an increase of the mean diameter of the building units within the macroaggregate. Ultramicroporosity (< 7 µm) is characterized by an upper limit of 40%. Microporosity (7–30 µm) and the sum of pores > 30 µm strongly correlate to microaggregate content and the land use type. A 1.5-fold enhancement of microaggregation increases a microporosity by three times. Pores > 30 µm are shaped by two factors: nearly 5% of these pores are biopores attributed to the high activity of soil fauna and roots, and therefore, this percentage is reduced in arable aggregates. The second part of pores > 30 µm is linked to the content of microaggregates and is the packing porosity of micro- within macroaggregates. Therefore, we have shown that the aggregate architecture under air-dry conditions described by µCT determines the subsequent breakdown of the aggregates under the slow moistening.

Soil fauna drives vertical redistribution of soil organic carbon in a long-term irrigated dry pine forest.

Summer droughts strongly affect soil organic carbon (SOC) cycling, but net effects on SOC storage are unclear as drought affects both C inputs and outputs from soils. Here, we explored the overlooked role of soil fauna on SOC storage in forests, hypothesizing that soil faunal activity is particularly drought‐sensitive, thereby reducing litter incorporation into the mineral soil and, eventually, long‐term SOC storage.In a drought‐prone pine forest (Switzerland), we performed a large‐scale irrigation experiment for 17 years and assessed its impact on vertical SOC distribution and composition. We also examined litter mass loss of dominant tree species using different mesh‐size litterbags and determined soil fauna abundance and community composition.The 17‐year‐long irrigation resulted in a C loss in the organic layers (−1.0 kg C m−2) and a comparable C gain in the mineral soil (+0.8 kg C m−2) and thus did not affect total SOC stocks. Irrigation increased the mass loss of Quercus pubescens and Viburnum lantana leaf litter, with greater effect sizes when meso‐ and macrofauna were included (+215%) than when excluded (+44%). The enhanced faunal‐mediated litter mass loss was paralleled by a many‐fold increase in the abundance of meso‐ and macrofauna during irrigation. Moreover, Acari and Collembola community composition shifted, with a higher presence of drought‐sensitive species in irrigated soils. In comparison, microbial SOC mineralization was less sensitive to soil moisture. Our results suggest that the vertical redistribution of SOC with irrigation was mainly driven by faunal‐mediated litter incorporation, together with increased root C inputs.Our study shows that soil fauna is highly sensitive to natural drought, which leads to a reduced C transfer from organic layers to the mineral soil. In the longer term, this potentially affects SOC storage and, therefore, soil fauna plays a key but so far largely overlooked role in shaping SOC responses to drought.

Boosting C Sequestration and Land Restoration through Forest Management in Tropical Ecosystems: A Mini-Review

Soil has a major role in sequestering atmospheric CO2. This has further benefits and potential to improve soil fertility and food production, mitigate climate change, restore land degradation, and conserve ecosystem biodiversity. However, its health is increasingly being threatened by the growing population, land degradation and climate change effects. Despite its importance, soil organic carbon (SOC) is understudied in the tropics. This paper reviews how managing forests in tropical ecosystems can benefit SOC sequestration and land restoration. Sequestered SOC has the potential to improve soil fertility, as well as to reduce both land degradation and atmospheric CO2 emissions. It further improves soil structure, aggregation and water infiltration, enhances soil faunal activity and boosts nutrient cycling (C, N, P and S). Managing forest ecosystems is crucial to boost C sequestration, mitigate climate change and restore degraded lands, besides other ecosystem services they provide. Apart from managing natural forests and planted forests, afforesting, reforesting marginal or degraded lands especially when associated with specific practices (organic residue management, introducing nitrogen-fixing species) boost C storage (in both soil and biomass) and foster co-benefits as soil health improvement, food production, land restoration and mitigation of climate change. Improved soil health as a result of sequestered C is confirmed by enhanced physical, biological and chemical soil fertility (e.g., sequestered C stability through its link to N and P cycling driven by soil biota) which foster and sustain soil health.

Nitrogen Enriched Organic Fertilizer (NEO) and Its Effect on Ryegrass Yield and Soil Fauna Feeding Activity under Controlled Conditions

This study aimed to investigate the effects of a new nitrogen-enriched organic-based fertilizer (NEO) on Italian ryegrass (Lolium multiflorum Lam.) yield and soil fauna feeding activity. Nitrogen is transformed from the air to manure by a plasma process. At the farm level, NEO could improve self-sufficiency and sustainability. The work was carried out under controlled conditions in two pot trials. Five fertilization regimes were used: no fertilizer, different amounts of mineral fertilizer, three NEO types, organic fertilizer (untreated manure), and organic fertilizer + different amounts of N in mineral fertilizer, including 14 treatments in trial one and 11 treatments in trial two. Besides evaluating dry matter yields, we utilized the Bait-lamina test system to assess the feeding activity of soil fauna. The results indicated a clear positive impact of nitrogen (N) on ryegrass yield where all fertilizers increased the yield in correspondence with their N availability regardless of the fertilizer type; whereas the yield was highest with mineral fertilizer up to our maximum level of 235 kg N ha−1 in trial one and 175 kg N ha−1 in trial two. The NEO fertilizers yielded in the same range as mineral fertilizers. The same clear pattern was not observed for soil fauna feeding activity. Instead, a tendency was observed where no fertilization tends to give the highest feeding activity. We saw no correlation between the yield and the soil fauna feeding activity. The feeding activity was highest in depth below 5 cm from the soil surface. Feeding activity also increased over time after fertilization. The NEO fertilizers had no more adverse effects on soil fauna feeding activity than other fertilizers. Other factors than fertilization alone are determining the soil fauna feeding activity.