Soil Extraction Tests Research Articles

Investigation of pore water and soil extraction tests for characterizing the fate of poorly soluble metal-oxide nanoparticles

Here we compared the efficiency of Cu extraction (dissolved + particulate) from two soils dosed with CuO nanoparticles (NPs) at 50 or 250 mg kg−1 by pore water collection, and single- and multi-step soil extraction tests. Pore water collection recovered low levels of Cu (<0.18%, regardless of soil type or Cu dose). Single soil extraction by either CaCl2 or DI water led to higher Cu recovery than pore water collection, but still <3% of total dose. These methods were useful for assessing the labile Cu ions pool. This fraction is controlled by Cu2+ dissolved from CuO NPs and it varies with time and soil type. Particulate Cu was poorly retrieved (<0.7%) by pore water extraction and by single-step soil extraction using CaCl2 solution or water. Multi-step extraction including dispersing and metal-chelating agents allowed for simultaneous characterization of dissolved Cu (total ionic Cu2+, 24–49% of dosed Cu), extractable CuO NPs (reversibly attached, 15–26% of dosed Cu), and non-extractable CuO NPs (irreversibly attached, 36–50% of dosed Cu), and it could describe the aging of NPs along 30 d. This method extracted a significantly higher concentration of Cu than pore water collection and was less sensitive to method parameters (e.g. filtration). This multi-step method can reduce pore water extraction-related factors that may confound the interpretation of environmental exposure data in NPs studies, and describe upper limits of both exchangeable Cu2+ and dispersible CuO NPs in soil that can potentially become bioavailable to plants and organisms and thus provide a sounder basis for risks evaluations.

Sulfur-modified biochar as a soil amendment to stabilize mercury pollution: An accelerated simulation of long-term aging effects

The stability of mercury (Hg) contamination in soil environments can change over time. This has implications for agricultural sites under long-term management after in situ treatment involving soil amendments. In this study, rice husk biochar (RHB) and sulfur modified rice husk biochar (SRHB) were synthesized and applied (dosage = 5% dry wt.) to a Hg polluted agricultural soil collected from Guizhou province, Southern China (soil total Hg content = 28.3 mg/kg; C = 2%; and, S = 0.1%). The long-term stabilization effectiveness of the soil treatments was evaluated by a combined approach involving: (i) accelerated aging for 104 simulated years; (ii) soil extraction as a proxy for plant uptake; and, (iii) sequential extraction to identify Hg fractions. The SRHB amendment raised the soil’s total S content by approximately an order of magnitude (to 0.9%), which remained at a generally constant level throughout the simulation. The initial pH levels for the untreated and treated soils were alkaline and remained between 7.0 and 7.5 for the first 50 years of simulated aging, before decreasing as the simulation time increased further. The pH of the SRHB treated soils did not drop below that of untreated soils during the simulation. Soil extraction tests with 0.1 M HCl solution indicated that RHB and SRHB treatments could effectively immobilize the Hg in soil for at least 50 and 75 simulated years, respectively. At simulated year 50, the amount of Hg extracted from RHB and SRHB treated soils was <200 ng/L and <100 ng/L, respectively. Thus, showing SRHB to be a particularly promising remedial option. The soil Hg was mostly associated with the stable sequential extraction fractions (F3-5). By the end of the simulation, the F5 fraction for SRHB and RHB treated soils reduced by 44.6%, and 42.0%, respectively, whereas the F4 fraction increased by >400% in both cases. In summary, SRHB may provide long-lasting Hg stabilization at contaminated sites. Therefore, further research toward the development of this stabilization technology is warranted.

Calibration of Mehlich-3 with Bray P1 and Ammonium Acetate in the Tri-State Region of Ohio, Indiana and Michigan

ABSTRACT Field crop fertilizer recommendations for Ohio, Indiana and Michigan are currently based on the Bray P1 extractant for phosphorus (P) and the ammonium acetate extractant (AA) for base cations. The fertilizer recommendations in this Tri-State region are currently being revised and will use the Mehlich-3 soil test extractant as the new basis for P and potassium (K) fertilizer recommendations. The goal of this study was to document the relationships between Mehlich-3, Bray P1, and AA soil test extractants and to provide a comprehensive review of these relationships published in the literature. Soil samples (n = 2,659) were collected across Ohio and Indiana from a diverse range of fields and analyzed for Mehlich-3, Bray P1 and AA extractable nutrients for P, K, calcium (Ca), and magnesium (Mg). Mehlich-3 P values were highly related to, but 35% greater than Bray P1 values. Mehlich-3 values were highly related to AA values, but 14% greater than AA-K, 13% greater than AA-Ca and 20% greater than AA-Mg. Our results are largely consistent with a comprehensively compiled literature review that indicates Mehlich-3 is an efficient and suitable soil test extractant for assessing extractable nutrient levels in the Tri-State region of Ohio, Indiana and Michigan.

Biochar Soil Amendment Effects on Arsenic Availability to Mountain Brome (Bromus marginatus )

Biochar is a renewable energy byproduct that shows promise for remediating contaminated mine sites. A common contaminant at mine sites is arsenic (As). In this study, the effects of biochar amendments to a mine-contaminated soil on As concentrations in mountain brome ( Nees ex Steud.) were investigated. In the biochar-amended soil, mountain brome had greater root biomass and decreased root and shoot As concentrations. X-ray absorption near-edge structure spectroscopy results showed that arsenate [As(V)] is the predominant species in both the nonamended and biochar-amended soils. Soil extraction tests that measure phosphate and arsenate availability to plants failed to accurately predict plant tissue As concentrations, suggesting the arsenate bioavailability behavior in the soils is distinct from phosphate. Results from this study indicate that biochar will be a beneficial amendment to As-contaminated mine sites for remediation.

Potassium availability and soil extraction tests in agricultural soils with low exchangeable potassium content

Low application of fertilizer potassium (K) in intensive agriculture leads to a gradual decrease and, afterwards, to a steady state of soil K determined by tests which are based on soil exchangeable K (Kexch) extraction. In this situation, non-exchangeable K (Knon-exch) is being released and therefore real plant availability does not necessarily correspond to soil test K. This incoherency was investigated in 14 agricultural soils with low K as determined by the Mehlich 3 method. Plant-available K released by exhaustive cropping of perennial ryegrass and K determined by the Neubauer seedling test were compared with 8 soil K extraction methods, with the total K content and with the relative content of soil K-bearing minerals. K determined by the ryegrass test (314 mg/kg on average) was more than 3-times higher than K based on Kexch extraction methods, but was from 2 to 3 times lower than K determined by Knon-exch extracting methods. The relative content of mixed-layer phyllosilicates was significantly related to K extracted by soil tests. The relative content of orthoclase correlated only with total K and mica-group minerals with none of the extractions. The best prediction of plant-available K in investigated soils was obtained with sodium tetraphenylboron and StepK methods.

Potassium availability and soil extraction tests in agricultural soils with low exchangeable potassium content

Potassium availability and soil extraction tests in agricultural soils with low exchangeable potassium content

Mercury in the Urban Topsoil of Athens, Greece

The present study documents the Hg content in 45 urban topsoil samples from the highly urbanized city of Athens, Greece. The Hg concentrations were quantified by applying aqua regia digestion on the &lt;100 μm soil fraction followed by inductively coupled plasma-mass spectrometry (ICP-MS) with a detection limit of 5 μg·kg−1. The median concentration of Hg in Athens soil is 96 μg·kg−1; ten out of 45 soil samples were found to contain Hg concentrations higher than 200 μg·kg−1, which is the maximum concentration value expected to be present in normal uncontaminated soils. Results obtained by multivariate principal component and hierarchical cluster analysis incorporating a large suite of chemical elements were notably effective for elucidating the anthropogenic origin of Hg in the studied soil. The elevated concentrations are most likely related to site-specific point source contamination rather than to the widely documented influences from the vehicular traffic emissions in urban settings. Given the proximity of urban population to the contaminated urban soils, we suggest the implementation of different soil extraction tests with the aim to evaluate the fraction of soil Hg available for absorption by the human body.

Suitability of Different Soil Extractants for Determination of Available Cu and Mn Contents in Polish Soils

The aim of this study was to compare four micronutrient soil-test extractants currently in common use in Poland, Rinkis, Mehlich 3, modified Lindsay and Norvell, and Yanai, for ability to determine the plant-available concentration of copper (Cu) and manganese (Mn) in two acidic soils (pH 4.8–4.9) that had been amended with different doses of Cu and Mn fertilizers. The comparison was based on two pot experiments (each with a different soil) with corn (Zea mays), barley (Hordeum vulgare), spring wheat (Triticum aestivum), rape (Brassica napus), red clover (Trifolium pratense), and English ryegrass (Lolium perenne). The extractants were evaluated based on a correlation analysis of the microelement concentrations in plants at the end of the vegetative phase and micronutrient concentration in the fertilized soil determined by each extractant at the end of the experimental period. The largest extractable concentrations of Cu and Mn in soils were obtained with Rinkis. The modified Lindsay and Norvell soil test extracted the lowest amount of both microelements. For soil Cu, the results with the Mehlich 3 and Yanai extractants were highly correlated with the Rinkis procedure results (r = 0.98). The modified Lindsay and Norvell results were not as well correlated with Rinkis (r = 0.84). For soil Mn, the correlation of results of Yanai, Mehlich 3, and modified Lindsay and Norvell to Rinkis ranged from r = 0.80 to r = 0.86. Levels of Mn and Cu measured with each of the extractants were highly correlated with plant tissue concentrations of Mn and Cu in most of the six plant species. The exceptions to this were the following: The modified Lindsay and Norvell soil Cu levels were not correlated with ryegrass and wheat plant Cu levels; Mehlich 3 Cu was not correlated with red clover Cu; and the Rinkis-extractable Mn was not correlated with the wheat Mn. It is concluded that the Yanai and Mehlich 3 procedures are suitable alternatives to the Rinkis for Cu but not for Mn.

Rapid and effective decontamination of chlorophenol-contaminated soil by sorption into commercial polymers: Concept demonstration and process modeling

Solid phase extraction performed with commercial polymer beads to treat soil contaminated by chlorophenols (4-chlorophenol, 2,4-dichlorophenol and pentachlorophenol) as single compounds and in a mixture has been investigated in this study. Soil-water-polymer partition tests were conducted to determine the relative affinities of single compounds in soil-water and polymer-water pairs. Subsequent soil extraction tests were performed with Hytrel 8206, the polymer showing the highest affinity for the tested chlorophenols. Factors that were examined were polymer type, moisture content, and contamination level. Increased moisture content (up to 100%) improved the extraction efficiency for all three compounds. Extraction tests at this upper level of moisture content showed removal efficiencies ≥70% for all the compounds and their ternary mixture, for 24 h of contact time, which is in contrast to the weeks and months, normally required for conventional ex situ remediation processes.A dynamic model characterizing the rate and extent of decontamination was also formulated, calibrated and validated with the experimental data. The proposed model, based on the simplified approach of “lumped parameters” for the mass transfer coefficients, provided very good predictions of the experimental data for the absorptive removal of contaminants from soil at different individual solute levels. Parameters evaluated from calibration by fitting of single compound data, have been successfully applied to predict mixture data, with differences between experimental and predicted data in all cases being ≤3%.

Comparison of Soil Test Extractants for Available Soil Phosphorus in High pH Sandy Soils of South Florida

This document addresses the selection of soil nutrient extractants in high pH soils and discusses their relationship to both nutrition and fertilizer management. This document’s objective is to describe the impact of selected soil extractants on nutrient management and their ability to determine soil phosphorus availability. The target audience for this series dealing with citrus nutrition includes Certified Crop Advisers; citrus, vegetable, and sugarcane producers; fertilizer dealers; and other parties interested in crop fertilization practices. This 4-page fact sheet was written by Kelly T. Morgan and Kamal Mahmoud, and published by the UF Department of Soil and Water Science, December 2013.

Comparison of Three Micronutrient Soil-Test Extractants in Three Greek Soil Types

The purpose of the present study was to compare the ability of three micronutrient soil-test extractants [diethylenetriaminepentaacetic acid (DTPA), Mehlich 3, and Soltanpour and Schwab] to determine plant-available concentrations of manganese (Mn), iron (Fe), and zinc (Zn) in three soils (from parent material Marl, Gneiss schist, and Peridotite) from central Macedonia, northern Greece. In black plastic bags containing 3 kg of air-dried soil, self-rooted olive plants (cv. Chondrolia Chalkidikis) were grown for about 5 months and irrigated with distilled water during the experimental period. At the end of the experimental period, the three extractants were evaluated, based on correlation analysis among leaf micronutrient concentrations, total plant micronutrient content of olive plants, and soil micronutrient concentrations determined by each extractant. The largest extractable concentrations of Mn, Fe, and Zn were determined by Mehlich 3, compared to the other two soil-test extractants. However, for the correlation analysis, the greatest correlation coefficient between leaf Mn (and total plant Mn content) and soil extractable Mn was achieved when DTPA was used (varying from 0.76 to 0.88, depending on soil type). Therefore, it is concluded that DTPA was a better extractant to determine plant-available Mn than the other extractants for the three soils studied. For correlations between leaf Fe and Zn concentrations and also for total plant Fe and Zn content, and soil extractable concentrations, the type of extractant and soil type play a very important role in determining the best correlation. This means that in each soil type the greatest correlation was achieved with the use of other extractant. For example, for Fe in the Marl and Peridotite soils the best correlation was found for Mehlich 3, whereas in the Gneiss schist the best correlation was achieved for DTPA (R = 0.72–0.94). For Zn, in the Gneiss schist soil the best extractant in determining plant available concentration was Soltanpour and Schwab (R = 0.49–0.60), whereas in the other two soil types DTPA was found to be the most reliable extractant (R = 0.51–0.78). Therefore, soil type should be carefully and thoroughly studied by the researchers in similar future experiments.

Orthophosphate and phytate extraction from soil components by common soil phosphorus tests

Chemically extractable phosphorus (P) is widely used for environmental risk assessment, because of its strong relationship with P loss through surface runoff and/or subsurface leaching. We used five common soil test extractants, water, 0.01M CaCl2, Mehlich I, Mehlich III and acidified ammonium oxalate in darkness (AOD) to recover two major P forms, orthophosphate (Pi) and phytate (inositol hexakisphosphate or IHP), which were adsorbed at various P saturation levels on Na-saturated goethite, gibbsite, kaolinite and montmorillonite. Our objective was to evaluate how the P form and mineral type influence the efficiency of these extractants to solubilize P and to examine the inter-correlations between extractable P fractions and the implications in P risk assessment. Phytate had a greater Langmuir adsorption maximum than Pi for all the studied minerals, whereas IHP had a lower extractability than Pi by all extractants. Phosphorus extractabilities increased in the order goethite<gibbsite<kaolinite<montmorillonite. Water and 0.01M CaCl2 extracted negligible IHP from all P saturation levels of these minerals. Mehlich III and AOD were equivalent in terms of the amount of P extracted. Mehlich III-P showed the best prediction of AOD-P, which is used for estimating soil degree of P saturation (DPS), whereas Mehlich I had a close relationship with AOD-P within a P form. The relationship between DPS estimated by AOD extraction and the true surface P saturation and the factors affecting the relationship were discussed in the model systems.

Predicting Need for Phosphorus Fertilizer by Soil Testing During Seeding of Cool Season Grasses

Recent changes in soil testing methodology, the important role of P fertilization in early establishment and soil coverage, and new restrictions on P applications to turf suggest a need for soil test calibration research on Kentucky bluegrass (Poa pratensis L.), tall fescue (Festuca arundinacea Schreb), and perennial ryegrass (Lolium perenne L.). Greenhouse and field studies were conducted for 42 days to examine the relationship between soil test P levels and P needs for rapid grass establishment using 23 NJ soils with a Mehlich-3 extractable P ranging from 6 to 1238 mg·kg–1. Soil tests (Mehlich-1, Mehlich-3, and Bray-1) for extractable P were performed by inductively coupled plasma–atomic emission spectroscopy (ICP). Mehlich-3 extractable P and Al were measured to evaluate the ratio of P to Al as a predictor of need for P fertilizer. Kentucky bluegrass establishment was more sensitive to low soil P availability than tall fescue or perennial ryegrass. Soil test extractants Mehlich-1, Bray-1, or Mehlich-3 were each effective predictors of need for P fertilization. The ratio of P to Al (Mehlich-3 P/Al %) was a better predictor of tall fescue and perennial ryegrass establishment response to P fertilization than soil test P alone. The Mehlich-1, Bray-1, and Mehlich-3 soil test P critical levels for clipping yield response were in the range of 170 to 280 mg·kg–1, depending on the soil test extractant, for tall fescue and perennial ryegrass. The Mehlich-3 P/Al (%) critical level was 42% for tall fescue and 33% for perennial ryegrass. Soil test critical levels, based on estimates from clipping yield data, could not be determined for Kentucky bluegrass using the soils in this study. Soil testing for P has the potential to aid in protection of water quality by helping to identify sites where P fertilization can accelerate grass establishment and thereby prevent soil erosion, and by identifying sites that do not need P fertilization, thereby preventing further P enrichment of soil and runoff. Because different grass species have varying critical P levels for establishment, both soil test P and the species should be incorporated into the decision-making process regarding P fertilization.

Divergent Relationships of Phosphorus Soil Tests in Temperate Grassland Soils

Various soil tests are used to estimate phosphorus (P) availability for both crop uptake and potential loss to water. Conversion equations may provide a basis for comparison between different tests and regions, although the extent to which information can be interchanged is uncertain. The objective was to determine and quantify relationships between specific soil test extractants for samples taken annually in October and February over 4 years from four sites in each of eight soil series under grassland. The extractants comprised Mehlich‐3, Morgan, Olsen, Bray‐1, lactate–acetate, CaCl2 (1∶2 and 1∶10 soil–solution ratios), and resin. The results showed distinct relationships for each soil series, for which individual lines regression models (different intercepts and slopes) were superior to a single conversion equation across all soils. The ensuing difference between soils was large and ranged from 1.9 to 8.0 and 9.2 to 15.6 mg kg−1 P for Morgan and Olsen, respectively, at 20 mg kg−1 Mehlich‐3 P. Generally, the environmentally oriented tests CaCl2 and resin correlated best with Morgan. Some soil‐specific limitations were also observed. CaCl2 was less efficient than Morgan, and Morgan less efficient than Mehlich‐3 on a high Fe–P soil derived from Ordovician‐shale diamicton, compared with the general trend for other soils. This finding suggests that further disparity may arise where evaluation of critical, or other, limits across regions involves even a limited sequence of tests.

Soil Test to Predict the Copper Availability in Pasture Soils

The proportion of copper (Cu) that can be extracted by soil test extractants varied with the soil matrix. The plant‐available forms of Cu and the efficiency of various soil test extractants [(0.01 M Ca(NO3)2, 0.1 M NaNO3, 0.01 M CaCl2, 1.0 M NH4NO3, 0.1 M HCl, 0.02 M SrCl2, Mehlich‐1 (M1), Mehlich‐3 (M3), and TEA‐DTPA.)] to predict the availability of Cu for two contrasting pasture soils were treated with two sources of Cu fertilizers (CuSO4 and CuO). The efficiency of various chemical reagents in extracting the Cu from the soil followed this order: TEA‐DTPA>Mehlich‐3>Mehlich‐1>0.02 M SrCl2>0.1 M HCl>1.0 M NH4NO3>0.01 M CaCl2>0.1 M NaNO3>0.01 M Ca(NO3)2. The ratios of exchangeable: organic: oxide bound: residual forms of Cu in M1, M3, and TEA‐DTPA for the Manawatu soil are 1:20:25:4, 1:14:8:2, and 1:56:35:8, respectively, and for the Ngamoka soil are 1:14:6:4, 1:9:5:2, and 1:55:26:17, respectively. The ratios of different forms of Cu suggest that the Cu is residing mainly in the organic form, and it decreases in the order: organic>oxide>residual>exchangeable. There was a highly significant relationship between the concentrations of Cu extracted by the three soil test extractants. The determination of the coefficients obtained from the regression relationship between the amounts of Cu extracted by M1, M3, and TEA‐DTPA reagents suggests that the behavior of extractants was similar. But M3 demonstrated a greater increase of Cu from the exchangeable form and organic complexes due to the dual activity of EDTA and acids for the different fractions and is best suited for predicting the available Cu in pasture soils.

Comparison of Soil-Test Extractants for Phosphorus, Potassium, Calcium, Magnesium, Sodium, Zinc, Copper, Manganese, and Iron in Louisiana Soils

Soils of different geographic regions affect efficiencies of individual soil-test extractants. Recent effort on nutrient-management programs across the United States has promoted establishment of conversion equations between different soil-test extractants for evaluating nutrients in similar soils. This study was carried out to compare soil-extractable phosphorus (P) by strong Bray (Bray 2), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na) by 1 M ammonium acetate (pH 7.0), and zinc (Zn), copper (Cu), manganese (Mn), iron (Fe) by DTPA-TEA at pH 7.3 with those extracted by Mehlich 3 on 317 soil samples collected from all parishes of Louisiana. Mehlich 3-extractable P correlated (R 2 = 0.709, P > 0.01) with that extracted by Bray 2 but was, on average, about half of that by Bray 2. Soils with pH > 6.5 or with finer textures tended to yield larger differences between the two extractants for P. Mehlich 3 extractable cations were highly correlated with those of ammonium acetate (R 2 ≥ 0.923, P > 0.01). Extractable Mg was close to 1:1 relation between the two procedures with slightly higher K and Ca, but lower Na by Mehlich 3. No notable effect of soil pH and texture was observed on K, Ca, Mg, and Na comparisons. Extractable Zn and Cu by Mehlich 3 generally correlated with those by DTPA (R 2 ≥ 0.899, P > 0.01) but the correlations on Mn and Fe were not as high (R 2 ≤ 0.420). Soil texture had no effect on efficiencies of micronutrient extractions with Mehlich 3 and DTPA. Soils with pH > 6.5 affected Fe and Mn ratios of Mehlich 3 extraction over DTPA but not on those of Zn and Cu. These results suggest that conversion equations could be developed for P, K, Ca, Mg, Na, Zn, and Cu between Mehlich 3, Bray 2, ammonium acetate, and DTPA for Louisiana soils. Soil pH and textural factors may need to be considered for soil P conversions between Mehlich 3 and Bray 2.

Predicting Zn and Cu status in cereals – potential for a multiple regression model using soil parameters

Prediction of micronutrient deficiency normally involves soil extraction tests and correlations between soil extractable micronutrients and their concentration in plant tissues. The present study reports that the inclusion of soil properties in addition to soil extraction data represents a powerful tool for improving models for a simple determination of phytoavailable Zn and Cu. Soil and plant samples were collected in spring 2000 from 19 cereal fields in Central and Southeast Norway at sites with known micronutrient deficiency. Based on the sample information, the model approach used stepwise regression, applying actual plant Zn and Cu concentration as the response, and soil extractable Zn/Cu and physico-chemical properties of the soil as model predictors. A model involving citric acid extractable Zn coupled with selected soil properties could predict the concentration of Zn in plant tissues of barley and oats grown under field conditions with a significantly high precision (R2=0·96, P&lt;0·001). The soil parameters included in the model, and their importance for phytoavailability of Zn, were in the order: citric acid extractable Zn&gt;clay content&gt;total Zn&gt;pH&gt;total C&gt;total N. Inclusion of DTPA extractable Zn in the model, rather than citric acid, resulted in a slightly lower precision in the prediction of phytoavailable Zn (R2=0·86, P&lt;0·001). A corresponding model for phytoavailable Cu, determined by DTPA and soil parameters, gave less precise prediction (R2=0·59, P&lt;0·01). DTPA was more suitable for predicting phytoavailable Cu than citric acid. Plant Mn concentration could not be correlated to any of the extracting agents.

Comparison of soil test extractants used in mid-Atlantic United States

A study was conducted to develop conversion equations between the five soil test extractants commonly used by public and private soil testing laboratories serving clients in the Mid-Atlantic United States. Three hundred soil samples were selected from among the samples submitted to the University of Delaware Soil Testing Program for routine fertility analysis. The samples were extracted with Mehlich 1, Mehlich 3, Bray P1, 1 N pH 7.0 ammonium acetate and 0.1 N HCl and analyzed for phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn), copper (Cu), and iron (Fe), as appropriate to the method. Significant linear relationships suitable for use as conversion equations were obtained between all extractants across a wide range of soil concentrations. Analysis of the 95% confidence bounds around the predictive equations showed large confidence intervals for some nutrient–extractant combinations (e.g., P, Mn, and Fe). Separation of the data into sub-populations based upon nutrient concentration (e.g., Mehlich 3 P<or>200 mg P dm− 3) and subsequent statistical analysis reduced the width of the confidence interval for some, but not all, relationships. For nutrient–extractant combinations where multiple relationships have been produced for specific concentration ranges, selection of the appropriate equation should be made based upon the intended application of the resulting data and the relevant concentration range (e.g., development of agronomic nutrient recommendations would require accuracy at “low P” concentrations versus limiting P application might require accuracy at “high” P concentrations). Statistical analysis of the data set using regression analysis with the “no intercept” option produced simple conversion factors that could also be used to rapidly and easily convert the results obtained with one soil test to those of another extractant. These “simple conversion factors” had slopes and confidence intervals that were comparable to those produced using standard regression analysis. The absence of an intercept component in the resulting relationship, however, would make these conversion factors more simplistic and less cumbersome for clients to use when comparing soil test results.

Comparison of Phosphorus Soil Test Extractants for Plant Availability and Environmental Assessment

A number of soil tests have been proposed to predict crop response to added P or to assess potential for soil P loss to runoff waters. A series of four separate experiments were conducted over a 10‐yr period to evaluate soil test methods on a total of 163 Vermont and New York field soils. The experiments included the following: (i) a pot study with alfalfa grown in the greenhouse with 31 soils either unfertilized or fertilized with 18 mg P kg−1; (ii) routine chemical analysis on 54 soils; (iii) a 360‐d incubation study with 24 soils receiving either 0, 20, or 40 mg P kg−1 as CaH2PO4, in which soils were analyzed for desorption and adsorption and the equilibrium P concentration (EPC0); and (iv) another set of 54 agricultural soils incubated with 0 or 40 mg P kg−1 and analyzed for CaCl2, distilled water, and ammonium acetate (Vermont 1)–extractable P (VT1P) and EPC0 Although P extracted by VT1 was significantly correlated with P removed by F extractants, it was better correlated with the ratio of F‐extractable P/Al extracted by either acetate or F. Phosphorus additions increased VT1P, as well as P extracted by acetate + F (Vermont 2 [VT2]), and they decreased reactive soil Al (VT1Al) and P adsorption. The amount of P needed to increase VT1P by a certain amount was directly related to the amount of Al in the VT1 extract. Phosphorus availability to plants, CaCl2‐extractable P, and the EPC0 were all more closely related to VT1P than P extracted by solutions containing F, such as Mehlich 3 (M3), Bray and Kurtz 1 (BK1), and VT2. In a number of instances the ratio VT2P/VT1Al had a better relationship with CaCl2P and EPC0 than did VT1P. Thus, the fraction of reactive Al that has reacted with P (as estimated by VT1P or the ratio of VT2P/VT1Al) appears to be a better indicator of P availability and potential P desorption to runoff water than is P extracted with F.

A comparison of plant available nutrients on decomposed granite cut slopes and adjacent natural soils

Decomposed granite (DG) slopes are often difficult to revegetate after existing topsoil and vegetative cover have been removed. In this study, naturally vegetated soils and denuded DG cut slopes were evaluated to compare edaphic (soil-related) conditions that may differ between the vegetated and non-vegetated states. Potential plant nutrient deficiencies were evaluated by soil nutrient extraction, plant tissue analysis and by greenhouse bioassay methods. The DG cut slopes have approximately one-quarter to one-half the clay content, soil organic matter, water-holding capacity, and total and available nitrogen (N) of the adjacent natural soils. Soil extraction tests for phosphorus (P) availability gave variable results, but a growth response to P amendment was observed in a greenhouse bioassay. The native topsoils and the DG cut slope materials had non-limiting levels of pH, exchangeable Ca, Mg and K, and total P. Cation exchange capacity and sulfate–sulfur (S) were low but similar between the two soil groups. Principle nutrient limitations are interpreted to be N, P, and possibly S and boron (B), in order of decreasing severity. Based on these results, we conclude that the removal of humified soil organic matter on the cut slopes reduces plant growth directly by lowering several of these essential nutrients, and indirectly by decreasing the soil's water-retention and infiltration capacity. Soil organic matter is viewed as a critical factor and major contrast between barren and revegetated DG materials. © 1998 John Wiley & Sons, Ltd.