Soil erosion by water on agricultural hillslopes leads to numerous environmental problems including reservoir sedimentation, loss of agricultural land, declines in drinking water quality, and requires deep understanding of underlying physical processes for better mitigation. It is imperative to accurately predict soil erosion caused by overland flow processes so that soil conservation efforts can be undertaken proactively before large-scale sedimentation problems arise. Soil detachment is often described by the excess shear stress equation that contains two physical soil erodibility parameters, erodibility coefficient, and critical shear stress. These parameters are normally assumed to be constant but can change across varying soil texture classes as well as during surface runoff events due to changes in soil cohesion and potential dependency on soil moisture content. These changes may significantly affect soil erosion rates at the field and watershed scale. In this study, the erodibility parameters of three soil types (sandy loam, clay loam, and silty clay loam) were analyzed using a laboratory mini-Jet Erosion Test (JET) to determine the effect of soil sample infiltration and moisture condition. Results from the experiments depicted a dynamic relationship between the soil erodibility parameters and amount of infiltrated mass of water. Data analysis displayed that for soils of different texture critical shear stress exhibited local minimum with higher values for very dry and saturated soils, while erodibility coefficient tended to increase with the increase of mass of soil water. Utilizing these dynamic soil erodibility parameters did not result in a significant difference in soil erosion rates when compared to using the averaged soil erodibility parameters taken from the experiment but the range of potential erosion rates increases with the increase of applied sheer stress to soil surface. The erosion rates with the experiment-based coefficients were found to be higher than with the baseline WEPP-based coefficients. These results highlight the importance of evaluating the effect of intrastorm dependent factors during surface runoff events, such as antecedent soil moisture content, time to peak from the start of runoff, soil cohesion, etc., on soil erodibility parameters to accurately calculate erosion rates, especially for initially dry soils or during earlier stages of surface runoff when critical shear stresses were highly affected. Further assessment of such factors with JET or other laboratory and field tests is recommended.