Plant microbial fuel cell (PMFC) is an emerging technology, showing promise for environmental biosensors and sustainable energy production. Despite its potential, PMFCs struggle with issues like low power output and limited drought resistance. Recent studies proposed that integrating biochar may enhance PMFC performance due to its physicochemical properties. The influence of different biochar types on PMFC efficiency has been minimally explored. This study aims to fill this gap by evaluating the performance of PMFCs integrated with various biochar types under unsaturated soil conditions. The study found that the addition of biochar types─specifically reed straw biochar (RSB), apple wood biochar (AWB), and corn straw biochar (CSB)─significantly influenced the performance of PMFCs. RSB, with its large surface area and porous structure, notably increased the current output by reducing soil resistance and enhancing electron transfer efficiency in microbial reduction reactions, achieving a peak power density of approximately 1608 mW/m2. AWB, despite its less porous structure, leveraged its high cation exchange capacity and hydrophilic functional groups to foster microbial community growth and diversity, thereby also increasing bioelectricity output. Conversely, CSB, with its large surface area, showed the least improvement in PMFC performance due to its layered structure and lower water retention capacity. Additionally, under drought conditions, PMFCs with added RSB and AWB exhibited better drought resistance due to their ability to improve soil moisture characteristics and enhance soil conductivity. The addition of biochar reduced soil resistance, increasing the bioelectric output of PMFCs and maintaining good performance even under low moisture conditions. This study highlights the critical role of biochar's surface area and functional groups in optimizing PMFC performance. It enhances our understanding of PMFC optimization and might offer a novel power generation method for the future, while also presenting a fresh strategy for soil monitoring.