Soil CO2 Efflux Research Articles

Understanding Soil Respiration Dynamics in Temperate Forests in Northwestern Mexico

Temperate mixed forests in Mexico are considered highly important ecosystems because of their high levels of biodiversity and capacity to store carbon. The aim of this study was to evaluate temporal and between-forest soil respiration (CO2 efflux) variability, and to assess the effect of vegetation diversity metrics on soil CO2 fluxes in mixed-uneven-aged forests in Durango, Northwestern Mexico. Soil CO2 efflux, soil moisture, and soil temperature were measured in three temperate forest types. A generalized linear model (GLM) was fitted to analyze the relationship between soil CO2 fluxes and stand variables, diversity metrics, soil moisture, and soil temperature. Furthermore, a two-way analysis of variance was used to assess the effect of forest type, month of the year, and their interaction on soil respiration. Annual average, minimum, and maximum soil CO2 efflux rate values were 3.81 (±2.94), 2.28 (±1.47), and 7.97 (±2.94) µmol m−2 s−1, respectively. Soil respiration was positively related to species richness, aboveground biomass, and quadratic mean diameter; however, forest type did not contribute to understanding the dynamics of soil CO2 fluxes. The results highlight the importance of seasonality, species diversity and aboveground biomass stocks to preserve the ecosystem processes driving soil respiration in temperate forests.

Responses of soil CO2 efflux and microbial activity to water deficit under conventional and adaptation technology

Frequent periods of low rainfall accompanying climate change are expected to affect many aspects of agricultural production and greenhouse gas emissions. In this work, we conducted field experiments on soil CO2 efflux and soil microbial activity using two precipitation treatments and two crop management technologies. While the control treatment was exposed to natural precipitation, the reduced water availability was induced during the early stages of crop growth using rain-exclusion shelters within the water deficit treatment. We aimed to test the difference in the response to water deficit between two management technologies: conventional tillage (CT - ploughed without cover crops) and adaptation technology (AT - no-till with species rich cover crops). After a month of simulated water deficit, the soil CO2 efflux under a regime of adaptation technology was essentially the same as that under the conventional technology, and the decrease in soil CO2 efflux ranged between 22 % and 55 %. The water deficit had a technology-specific effect on microbes. While no effect on the microbial carbon under CT was observed, the water deficit decreased Cmic under AT. In contrast, the opposite pattern was observed for DHA. When a normal soil water regime was restored, CO2 efflux recovered rapidly while recovery of microbial biomass and dehydrogenase activity was more variable. Generally, the soil microbial biomass and activity recovered better under AT. The results show that while the reduction in Rs during water deficit and its recovery after the restoration of the ambient precipitation regime were not affected by the technology, the response of microbial biomass and activity to water deficit depended on the technology with better recovery under AT.

Precipitation patterns and N availability legacy govern microbial response to rewetting in a plant-soil system

Climate change models predict shifts in the frequency and magnitude of rain events (precipitation patterns). We studied how precipitation history shapes microbial community responses to rewetting and how these effects depend on N status. Twelve weeks of contrasting precipitation and N input left a legacy effect by shaping present (DNA-based) and potentially active (rRNA-based) bacterial and fungal communities. This legacy effect determined the microbial response to rewetting, as demonstrated by differences in the post-wet potentially active bacterial and fungal communities as well as the flux of soil-emitted CO2 over a 29-h period. Despite contrasting effects of precipitation and N input history on fungal:bacterial ratio and microbial community composition, the timing of the potentially active bacterial and fungal response to rewetting was not altered. Thus, regardless of precipitation or N input history, potentially active bacteria responded with a small shift in community composition within 1 h of rewetting but did not change further for the remaining 28 h analyzed. The potentially active fungi did not respond to rewetting within 29 h. Even though more extreme fluctuations in soil moisture changed soil microbial community composition, the short-term response of microbial communities to rewetting was conserved. Soil CO2 efflux upon rewetting was higher from systems with a history of frequent precipitation and underline the role of plants in drying-rewetting processes. We suggest that shifts in the fungal:bacterial ratio, as well as N-cycling potentials, may have consequences on food web stability and soil biogeochemical cycling.

When dry soil is re-wet, trehalose is respired instead of supporting microbial growth

When dry soil is re-wet there is a rapid increase in CO2 efflux and rates can remain above those of well-watered controls for one or more days. These large pulses of CO2 efflux are known as the “Birch effect”. To provide experimental evidence of different pools of C fuelling the Birch effect, we incubated a drying soil with 13C6-glucose, re wet the soil and quantified 13C labelling of pools (microbial biomass, trehalose, extracellular, and old C) and soil CO2 efflux. We took advantage of trehalose being the most 13C-enriched pool (δ13C = +518‰) to obtain direct isotopic evidence of trehalose's contribution to respiration and microbial growth. For soil incubated with 13C6-glucose, the δ13C of soil respiration was +35‰ in dry soil, increased to 100‰ in the 10 min following rewetting, and subsequently decreased. During the first 5 h after re-wetting, trehalose must have been contributing to respiration given that δ13C of soil respiration was more 13C enriched than trehalose-free microbial biomass (δ13C = +30‰), extracellular C (δ13C = −17.7‰), and old C (δ13C = −22.9‰). A four-member isotopic mixing model suggested trehalose underpinned 16% of respiration in the 1st hour after rewetting, decreasing to 7% in the fifth hour. At times beyond 5 h after rewetting, trehalose underpinned 0–4% of respiration. In the seven days following rewetting, microbial biomass increased 2292 nmol C g−1. Isotopic mass balance indicated trehalose-C could account for no more than 5% of the gross influx of C into microbial biomass, instead the increase in microbial biomass was fuelled by unlabelled or weakly labelled pools such as old C and extracellular C. Collectively these data provide direct experimental evidence C from trehalose does not significantly contribute to microbial growth in re-wet soil, but instead contributes to respiration for the first 5 h after rewetting.

Patterns and determinants of soil CO2 efflux in major forest types of Central Himalayas, India.

Soil CO2 efflux (Fsoil) is a significant contributor of labile CO2 to the atmosphere. The Himalayas, a global climate hotspot, condense several climate zones on account of their elevational gradients, thus, creating an opportunity to investigate the Fsoil trends in different climate zones. Presently, the studies in the Indian Himalayan region are localized to a particular forest type, climate zone, or area of interest, such as seasonal variation. We used a portable infrared gas analyzer to investigate the Fsoil rates in Himalayan tropical to alpine scrub forest along a 3100-m elevational gradient. Several study parameters such as seasons, forest types, tree species identity, age of trees, distance from tree base, elevation, climatic factors, and soil physico-chemical and enzymatic parameters were investigated to infer their impact on Fsoil regulation. Our results indicate the warm and wet rainy season Fsoil rates to be 3.8 times higher than the cold and relatively dry winter season. The tropical forest types showed up to 11 times higher Fsoil rates than the alpine scrub forest. The temperate Himalayan blue pine and tropical dipterocarp sal showed significant Fsoil rates, while the alpine Rhododendron shrubs the least. Temperature and moisture together regulate the rainy season Fsoil maxima. Spatially, Fsoil rates decreased with distance from the tree base (ρ = - 0.301; p < 0.0001). Nepalese alder showed a significant positive increase in Fsoil with stem girth (R2 = 0.7771; p = 0.048). Species richness (r, 0.81) and diversity (r, 0.77) were significantly associated with Fsoil, while elevation and major edaphic properties showed a negative association. Surface litter inclusion presented an elevation-modulated impact. Temperature sensitivity was exorbitantly higher in the sub-tropical pine (Q10, 11.80) and the alpine scrub (Q10, 9.08) forests. We conclude that the rise in atmospheric temperature and the reduction in stand density could enhance the Fsoil rates on account of increased temperature sensitivity.

Physical-hydric attributes and soil CO2 efflux in pastoral systems in a Brazilian semi-arid environment

Physical-hydric attributes and soil CO2 efflux in pastoral systems in a Brazilian semi-arid environment

Combined impact of no-tillage and cover crops on soil carbon stocks and fluxes in maize crops

Soil plays a major role in the carbon cycle for both carbon stocks and respiration since soil organic carbon (SOC) is the most important carbon pool and soil CO2 efflux is the largest source of carbon emissions to the atmosphere. The aim of this study was to understand how the combination of no-till (NT) and cover cropping (CC) influences both SOC and soil respiration. Soil respiration was measured in southwestern France from April to October at two distinct sites characterized by contrasting initial soil carbon contents. Each site included two adjoining maize fields in which conservation (NT+CC) and conventional (CT) practices were used. For soil with initial high SOC contents (> 50 tC ha-1 in the 0–30cm layer), SOC stocks at the 0–90 cm depth (NT+CC: 72.4 ± 10.6, CT: 68.9 ± 9.2 t C ha-1) and respiration during the maize growing season (NT+CC: 0.57 ± 0.24, CT: 0.54 ± 0.26 g CO2 m-2 h-1) were not significantly different for both practices. For soil with initial low SOC contents, SOC stocks at the 0–90 cm depth (NT+CC: 42.7 ± 5.5, CT: 29.7 ± 4.8 t C ha-1) and respiration (NT+CC: 0.86 ± 0.32, CT: 0.50 ± 0.34 g CO2 m-2 h-1) were higher with the NT+CC practices than with the CT practices. Due to higher cover crop biomass and root density, SOC mineralization and root respiration were enhanced. We showed a positive effect of conservation practices on the SOC stock in the upper layers but also in the deeper layers (60–90 cm) only in a low initial carbon content environment. At high SOC levels, the soil may have reached its carbon sequestration potential, or the time since practices were implemented was not long enough to observe the effects. Our study shows that the potential of NT+CC to sequester large amounts of SOC, enabling the mitigation of greenhouse gas emissions, strongly depends on initial SOC contents.

Seasonal Environmental Controls on Soil CO2 Dynamics at a High CO2 Flux Sites (Piton de la Fournaise and Mayotte Volcanoes)

AbstractEnvironmental parameters drive seasonal soil CO2 efflux toward the atmosphere. However, their influence is not fully understood in contexts of high CO2 fluxes where CO2 accumulates in the subsurface. A prime example are volcanoes subject to continuous CO2 diffuse degassing rising from deep magmatic reservoirs, through the subsurface and up to the atmosphere. For many of these volcanoes where soil CO2 is monitored, a seasonal influence of the atmosphere and water table is observed but not characterized. Here, we compare variations of air temperature, atmospheric pressure, rainfall and water table level with near‐surface soil CO2 concentration by performing a time‐lagged detrended cross‐correlation analysis on years‐long time series from the volcanoes of Piton de la Fournaise and Mayotte. At Piton de la Fournaise, soil CO2 variations correlate best with air temperature variations (0.81) and water table variations (0.74). In Mayotte, soil CO2 variations correlate best with atmospheric pressure variations (−0.95). We propose that at Piton de la Fournaise, the thick vadose zone and high permeability favor CO2 transfer by thermal convection. Additionally, energy transfer is decoupled from mass transfer. Slow heat transfer from the atmosphere down to the accumulated CO2 layers in the subsurface results in a delayed influence of air temperature and of the water table level on the thermal gradient between the subsurface and the atmosphere, and consequently on the efficiency of the CO2 transfer. In Mayotte, we propose that the thin vadose zone and the presence of a network of large fractures favor CO2 transfer by barometric pumping.

Biodegradable plastic mulch films increase yield and promote nitrogen use efficiency in organic horticulture

Plastic film mulches (PFM) are used extensively due to their ability to increase yield and suppress weed emergence. Their effects on plant-soil-microbial interactions, however, are less well understood. Organic systems rely on the supply of nutrients from organic sources (e.g., manures and fertility-building leys) and where poor N availability often limits yield. The issue is compounded by horticultural crops (e.g., lettuce) with a high N demand, but which are inefficient at recovering N from soil. The effect of PFM on the mineralisation of organic fertilisers and its interaction with other agronomic factors such as planting density is also less well-researched. We hypothesised that biodegradable PFM would be a useful tool to increase the efficiency of N management in organic horticulture by increasing the supply of available N leading to increased crop N uptake and crop yield, and simultaneously reducing N losses during the growing season. We conducted two field experiments under a temperate maritime climate with lettuces grown with either conventional (LDPE) PFM or a polylactic acid-based biodegradable PFM alongside un-mulched controls. The first experiment involved black or white coloured PFMs and two planting densities while the second experiment involved treatments with and without addition of poultry manure. Overall, yields were increased by 39% in both experiments with PFM, while soil mineral N concentrations were up to 5 times higher with PFM than without. Measurements of soil organic matter (SOM) turnover (Tea Bag Index) and soil CO2 efflux indicated a more rapid decay of SOM in the presence of the PFM. The use of PFM also promoted N use efficiency (NUE) by 300% in the presence of poultry manure. Denser planting with PFM resulted in proportionately higher yields. Higher yield and higher N concentrations (5-10%) in crop tissue in mulched plots resulted in higher total N uptake, however, total N uptake was low compared to soil concentrations: mulching with biodegradable PFM resulted in higher residual mineral N than un-mulched plots (77-147 mg kg-1 vs. 19 -70 mg kg-1). Our results are consistent with reduced N losses to the environment during the growing season and increased mineralisation under PFM. In conclusion, our findings support the adoption of PFM for organic horticulture and show that biodegradable PFM perform similarly to LDPE-based PFMs.

Soil CO2 efflux dynamics in an integrated crop–livestock system

AbstractIntegrated crop–livestock (ICL) systems have shown potential to provide a variety of environmental benefits including soil carbon (C) increases relative to conventional row cropping systems. However, studies documenting C dynamics of ICL systems in the northern Great Plains are lacking relative to other agroecosystems. Soil carbon dioxide (CO2) efflux, crop biomass, and soil organic matter (SOM) pools were monitored over 3 years in an ICL rotation (corn [Zea mays L.]/soybean [Glycine max L.]–spring wheat [Triticum aestivum L.] + cover crop–cover crop) with fall grazing, a conventional cropping system rotation (corn–spring wheat–soybean) and fall grazed and ungrazed mixed‐grass pasture near Mandan, ND. Cropped treatments were under no‐till management. Annual aboveground crop residue biomass C was similar in the ICL and conventional systems, while less in the grazed pasture (4.18, 3.83, and 1.21 Mg C ha−1 year−1 respectively; p = 0.039). Annual soil CO2 efflux was greater in the ICL, grazed, and ungrazed pasture systems than the conventional system (8.05, 8.73, 8.25, and 5.81 Mg C ha−1 year−1, respectively; p &lt; 0.001). Among crop phases in the ICL and conventional cropping systems, cover crops contributed to greater CO2 efflux in the spring and fall. SOM and C mineralization were greater at 0–5 cm in the ICL system compared to the conventional cropping system (6.6% vs. 6.3%, p = 0.028 and 248 vs. 184 mg CO2–C kg−1, p &lt; 0.001, respectively). Interseeded cover crops in ICL systems can contribute increased root respiration and enhanced SOM pools relative to conventional cropping systems under semiarid conditions.

Trend for Soil CO2 Efflux in Grassland and Forest Land in Relation with Meteorological Conditions and Root Parameters

The key process in understanding carbon dynamics under different ecosystems is quantifying soil CO2 efflux. However, this process can change annually as it depends on environmental variables. The results of this paper present the effects of root network, soil temperature, and volumetric water content on soil CO2 efflux, which were investigated on Retisol of two types of land uses in Western Lithuania in 2017–2019: forest and grassland. It was determined that the average soil CO2 efflux in the grassland was 32% higher than in the forest land. The CO2 efflux, average across land uses, tended to increase in the following order: 2017 &lt; 2018 &lt; 2019. Dry weather conditions with high temperatures during the vegetation period governed the soil CO2 efflux increase by 14%. Soil temperature (up to 20 °C) and volumetric water content (up to 23–25%) had a positive effect on the soil CO2 efflux increase on Retisol. We established that the root’s activity plays one of the main roles in the CO2 production rate—in both land uses, the soil CO2 efflux was influenced by the root length density and the root volume.

Estimating the response of Himalayan old-growth mountain forests to decreased monsoon precipitation

Forests in the Himalayas are a major carbon store, but are under threat due to changes in precipitation regime. To simulate a precipitation decline, throughfall-exclusion (TFE) shelters were applied during three consecutive monsoon seasons in an oak forest (2.650 m a.s.l.) and a conifer-dominated forest (3.260 m a.s.l.) in central Bhutan. Leaf water potentials, tree mortality, stem increment, soil CO2 efflux, litterfall and fine root dynamics were assessed. TFE significantly and consistently decreased topsoil (0–30 cm) moisture and leaf water potentials of Quercus lanata and Quercus griffithii (lower elevation), and to a lesser extend those of Tsuga dumosa and Quercus semecarpifolia, (higher elevation). TFE did not impose tree mortality. Stem increment remained unaffected until the second TFE year, but showed reductions during the third year with Tsuga dumosa being most severely affected (-60%). Standing fine root biomass stocks were hardly affected by TFE. Increased root necromass and faster fine root growth in the lower elevation forest suggest that the oak trees increased C allocation below ground. Soil CO2 efflux sharply declined during all three TFE years in both forests. Above ground litter input was unaffected by TFE until the second treatment year. Overall, both forest ecosystems appeared highly resistant to the imposed soil drying, with no signs of tree mortality and stable living root biomass stocks.

Fine scale assessment of seasonal, intra-seasonal and spatial dynamics of soil CO2 effluxes over a balsam fir-dominated perhumid boreal landscape

Total soil CO2 efflux (FCO2) is the second most important carbon flux after photosynthesis in boreal forests. However, accurate modelling of FCO2 remains challenging because of its high variability, both temporally and spatially. Using an Abies balsamea-dominated boreal landscape in Quebec (eastern Canada) as a case study, we modelled seasonal, intra-seasonal and spatial variability of FCO2 using climate variables and topographic and canopy structure attributes derived from Light Detection and Ranging (LiDAR) and assessed their respective contributions to soil CO2 emissions. Weekly point measurements of FCO2 at 99 sites were taken over an area of 122 ha between June and October 2020. The seasonal component of FCO2 was quantified and subtracted from FCO2 measurements to isolate the spatial and intra-seasonal components of the flux. The two components were then modelled using a Random Forest Regression model and studied using accumulated local effect plots (ALE plots). Our approach explained 81% of the variation in FCO2: the seasonal pattern explained 36% of the variation in FCO2 measurements, while spatial and intra-seasonal patterns together explained 45%. The most important factors explaining spatial variation were vegetation height and the slope height. Average air temperature of the last two days before efflux measurements was the most important factor explaining intra-seasonal variation. The proposed methodology makes it possible to predict FCO2 from external factors derived from climate and remote sensing data and enables the decomposition of FCO2 into its seasonal, intra-seasonal and spatial components. Our results demonstrate the importance of spatial and intra-seasonal variations in FCO2 compared to seasonal variation, a finding that has implications for the measurement and modelling of FCO2 at landscape and global scales.

Soil fertility determines whether ectomycorrhizal fungi accelerate or decelerate decomposition in a temperate forest.

Ectomycorrhizal (ECM) fungi can both accelerate and decelerate decomposition of organic matter in forest soils, but a mechanistic understanding of this differential influence is limited. Here, we tested how ECM fungi affect decomposition along a natural fertility gradient in a temperate forest of European beech. Trees were girdled to reduce belowground carbon supply to the soil. Girdling shifted soil fungal community composition and decreased hyphal biomass production and soil CO2 efflux, indicating a reduced ECM fungal activity. Girdling also affected decomposition processes, but the effects depended on fertility. Our results indicate that ECM fungi decelerate decomposition under conditions of low fertility while under conditions of high fertility ECM fungi and their host roots have an accelerating effect. We conclude that both acceleration and deceleration of decomposition of organic matter by ECM fungi can occur within a forest, with soil fertility determining the direction and magnitude of these effects. We suggest a positive feedback between fertility, stand productivity and soil carbon and nitrogen dynamics that is mediated to a large extent by ECM fungi.

Shallow water habitat management influences soil CO2 efflux from agricultural fields in the Lower Mississippi River Basin (LMRB), USA

AbstractTemporary winter flooding is a common practice for enhancing wildlife habitat in the Lower Mississippi River Basin (LMRB) and other migratory waterfowl flyways in North America. This practice also facilitates nutrient retention and reuse of rainwater‐ or drainage‐water. This study examines the consequence of five different water management approaches on soil carbon dioxide (CO2) efflux and corn (Zea mays L.) residue decomposition, from October 11, 2021, through January 31, 2022. Management treatments included (i) control (CT) or no flooding; (ii) passive (P) rainfall capture; (iii) fall (F) pumping of leftover irrigation water; (iv) winter (W) flooding with leftover irrigation water; and (v) fall–winter (FW) flooding. The fall–winter flood field (FW) had reduced daily soil CO2 efflux for 4 out of 12 observations and increased soil pH and residue decomposition compared to the control field. The winter flood field (W) had reduced CO2 efflux on December 8 and January 21, and FW had lower efflux on December 8, December 14, December 21, and January 13, than the control field (CT). Soil temperature was positively associated with soil CO2 efflux in all fields except control. Results are consistent with the hypothesis that flood‐timing and ‐duration had strong connections with soil carbon dynamics in LMRB row croplands during fallow periods.

The characteristics of ecosystem respiration and its components of a representative film-mulched and drip-irrigated cotton field in Northwest China

Ecosystem respiration (Re) is the largest terrestrial carbon flux into the atmosphere; thus, investigating its characteristics is a prerequisite for understanding the global carbon balance. Croplands are important in regulating regional and global carbon cycles, given their unique role as humans’ food source. The widely used film-mulched and drip-irrigation (FMDI) for the water-saving purpose has clear benefits for promoting crop production, but the impact of FMDI on Re and its components remains rarely explored. The objective of this study is to evaluate the characteristics of Re and its above-ground autotrophic (Raa), below-ground autotrophic (Rab), and heterotrophic (Rh) components in an FMDI cotton field in an oasis of Northwest China. To achieve this, we measured Net Ecosystem Exchange (NEE) with the atmosphere using the eddy covariance from 2012 through 2016 and partitioned it into Gross Primary Productivity (GPP) and Re using the nighttime method; in 2016, we used chamber-based technique to measure Rh and total soil respiration (Rs), and used their relationships with soil temperature and moisture to construct continuous records to estimate the annual soil CO2 effluxes for the five years. Results showed that the mean annual Re was 1340 g C m−2 yr−1, with Raa, Rab, and Rh accounting for 24%, 24%, and 52%, respectively. Rs was the dominant part of Re compared with the Raa; Rh accounted for 68% of Rs, and Rab accounted for only 32% of Rs due to the low root ratio. Our results suggested that the unique soil temperature and moisture conditions generated by FMDI generally stimulate heterotrophic respiration more than autotrophic respiration. This study contributes important knowledge of Re and its components in the FMDI cotton field and helps better understand how FMDI may impact the regional carbon balance.

Moisture, temperature and respiration of two soil classes under pasture and tropical dry forest in the semiarid Brazilian region

Soil respiration (Rs), or soil CO2 efflux, is the largest carbon flux from ecosystems to the atmosphere; it is an order of magnitude greater than that released by human activity, and is a key process in CO2 atmospheric accumulation and climate change. Due to its importance, many studies have investigated respiration in different ecosystems, with studies in tropical dry areas being more scarce. We measured the effects of soil temperature and moisture on respiration from Regosols and Luvisols under pasture and native deciduous forests in the Brazilian Northeast semiarid region. Respiration had a direct relationship with soil moisture and an inverse relationship with temperature in both soil classes and land covers. Respiration was higher under the forest than under the pasture (9.0 and 4.8 Mg C ha−1 y−1) in both wet and dry seasons, probably due to higher biomass incorporation and higher root respiration, but it did not differ between soil classes. These respirations corresponded to high proportions of the soil C contents in the 0–10 cm superficial layer (46.2% in Caatinga and 36.7% in pasture). In the Brazilian semiarid region, soil respiration was controlled by soil moisture (positively) and temperature (negatively), both of which were affected by land-use changes.

Functional variability in specific root respiration translates to autotrophic differences in soil respiration in a temperate deciduous forest

CO2 release from forest soils (Rs) is a prominent flux in the global carbon cycle. Rs is derived from roots (autotrophic respiration, Ra) and microbial (heterotrophic) respiration and is highly dynamic, as it depends on edaphic and environmental conditions as well as root functional traits and microbial community composition. It is unclear how root functional traits affect root and microbial respiration rates; however, their consideration may help parse out the relative contributions of root and microbial respiration to Rs. At a temperate forest site, root systems of 3–4 functional root orders and their surrounding surface soil were carefully excavated and placed into custom trays designed to repeatedly measure Rs in situ on eight temperate tree species that varied in their root functional strategies and mycorrhizal affinity. Rs was measured bi-weekly to monthly for nearly one year using a custom chamber attached to a gas exchange system. Rs varied over time, ranging from 0.3 to 12 µmol m−2 s−1. Comparable root systems of the same species were excised from the soil and specific root respiration rates (Rr) were measured. Rr ranged from 2.5 to 9.0 nmol g−1 s−1 and was negatively correlated with root tissue density and positively related to root tissue nitrogen concentration. Using Rr to estimate Ra, we estimate that Ra accounts for <10%, on average 2–3%, of Rs for individual root systems (averaging 1.2 g dry biomass) housed in surrounding soil (average 1.3 kg dry mass) in situ; thus, Ra was roughly 20 times greater than Rh per unit mass. The contribution of Ra peaked in the fall and coincided with leaf senescence of the forest canopy. A soil-sterilizing experimental treatment designed to help isolate Ra in situ reduced bacterial biomass and shifted fungal community composition, but there was no reduction in Rs of the in-situ root-soil tray systems. The relative Ra to Rs ratio increased with root functional strategies characterized by greater specific root length and tip abundance, but also to greater root tissue density. The ratio of Ra to Rs also increased with warmer soil temperatures and decreased slightly with increasing soil moisture. We discuss how incorporating root functional traits as modulators of the autotrophic contribution to Rs could be considered when modeling total soil CO2 efflux from forests.

Differences in CO2 Emissions on a Bare-Drained Peat Area in Sarawak, Malaysia, Based on Different Measurement Techniques

The drainage and cultivation of peatlands will lead to subsidence and mineralisation of organic matter, increasing carbon (C) loss as more CO2 is emitted. There is little information about carbon emissions from bare peat soil. A study was undertaken to measure the CO2 emissions from a logged-over peat swamp area that was purposely vegetation-free. We aimed to report CO2 emissions from a bare, drained peatland developed for an oil palm plantation. For 12 months, we used eddy covariance (EC), closed chambers, and soil subsidence measurements to derive CO2 emissions from a logged-over peat swamp area. Significant variations in the estimated soil CO2 efflux were observed in the three tested measurement techniques. The average CO2 flux rate measured by the EC technique was 4.94 ± 0.12 µmol CO2 m−2 s−1 (or 68.55 tonnes CO2 ha−1 year−1). Meanwhile, the soil CO2 efflux rate measured by the closed chamber technique was 4.19 ± 0.22 µmol CO2 m−2 s−1 (or 58.14 tonnes CO2 ha−1 year−1). Subsidence amounted to 1.9 cm year−1, corresponding to 36.12 tonnes CO2 ha−1 year−1. The estimation of the C loss was found to be highest by the EC technique, lower by the soil chamber technique, and lowest by the peat subsidence rate technique. The higher CO2 emission rate observed in the EC technique could be attributed to soil microbial respiration and decomposing woody residues in the nearby stacking rows due to the large EC footprint. It could also be affected by CO2 advection from oil palms adjacent to the study site. Despite the large differences in the CO2 emission rates by the different techniques, this study provides valuable information on the soil heterotrophic respiration of deep peat in Sarawak. Carbon emissions from a bare peat area cover only a fraction of the soil CO2 respiration component, i.e., the soil heterotrophic respiration. Further investigations are needed to determine the CO2 emissions by soil microbial activities and plant roots from other peat areas in Sarawak.

Modeling Soil CO2 Efflux in a Subtropical Forest by Combining Fused Remote Sensing Images with Linear Mixed Effect Models

Monitoring tropical and subtropical forest soil CO2 emission efflux (FSCO2) is crucial for understanding the global carbon cycle and terrestrial ecosystem respiration. In this study, we addressed the challenge of low spatiotemporal resolution in FSCO2 monitoring by combining data fusion and model methods to improve the accuracy of quantitative inversion. We used time series Landsat 8 LST and MODIS LST fusion images and a linear mixed effect model to estimate FSCO2 at watershed scale. Our results show that modeling without random factors, and the use of Fusion LST as the fixed predictor, resulted in 47% (marginal R2 = 0.47) of FSCO2 variability in the Monthly random effect model, while it only accounted for 19% of FSCO2 variability in the Daily random effect model and 7% in the Seasonally random effect model. However, the inclusion of random effects in the model’s parameterization improved the performance of both models. The Monthly random effect model that performed optimally had an explanation rate of 55.3% (conditional R2 = 0.55 and t value &gt; 1.9) for FSCO2 variability and yielded the smallest deviation from observed FSCO2. Our study highlights the importance of incorporating random effects and using Fusion LST as a fixed predictor to improve the accuracy of FSCO2 monitoring in tropical and subtropical forests.