With increasing nitrogen application in soil, the problem of nitrate accumulation in soil and vegetable bodies has become increasingly serious. In this study, Bacillus subtilis (B. subtilis) Strain11 was isolated and studied for its effects in combination with Molybdenum (Mo) on the growth of Chinese cabbage and soil nitrate content. The results showed that the strain effectively increased the growth, height, and moisture content of Chinese cabbage by 27% and 2.5%, respectively, compared to the control. Mo application alone reduced soil nitrate accumulation and increased root length, height, chlorophyll content, and moisture content of Chinese cabbage, with an optimal rate of 0.8–1.2 mg/kg soil. The application of Mo fertilizer in combination with the Bacillus strain was the most effective in increasing plant height and root length of Chinese cabbage, which were 1.49 times and 1.68 times that of the control, respectively. The proportion of dry matter, the average fresh weight, and the dry weight under this treatment were 9.01%, 5.83 g/plant, and 0.53 g/plant, respectively, higher than the rest. At harvest, the highest ammonium-nitrogen content in this treatment group was 8.56 mg/kg, and the soil nitrate reduction reached 40.68%. In conclusion, Mo fertilizer at a rate of 0.8 mg Mo/kg soil, in combination with B. subtilis Strain11, was recommended for the remediation of nitrate-contaminated soils.
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