Soil Biological Activity Research Articles

Sustaining Soil Biological Activity: The Role of Extended Reduced and No-Tillage Techniques

Soil management techniques can have varying effects on various soil properties. This study investigated the impact of various tillage techniques on soil properties for 14 years. The experiment was conducted at the Çukurova University Research Station, located in a region with a dominant Mediterranean climate. The research aimed to assess the changes in soil organic matter (SOM) content, soil respiration (SR), dehydrogenase enzyme activity (DHA), and soil temperature (ST) under seven different long-term tillage practices. The results revealed significant increases (p ≤ 0.05) in SOM (17-115%), SR (19-37%), and DHA (63-142%), under conservation tillage compared to conventional tillage practices. Additionally, conventional tillage with stubble burned consistently had the lowest values across all measured properties. Seasons variations also significantly (p≤0.05) affected the observed values. These findings suggest that conventional tillage practices have a negative effect on the analyzed biological activities, with stubble burning further exacerbating this impact. Further research exploring the long-term effects of different tillage practices under varying crop rotations and soil conditions can contribute to the sustainable development of agricultural production in the region.

Exploring the potential of horse amendment for the remediation of HCHs-polluted soils

This study assessed for the first time the bioremediation potential of an organic horse amendment in soils contaminated with solid wastes of the obsolete pesticide lindane (α−hexachlorocyclohexane (α−HCH) = 80 mg kg−1, β−HCH = 40 mg kg−1, γ,δ,ε−HCH≈10 mg kg−1) searching for a self-sufficient bio-based economy. Four treatments were implemented: polluted (PS, ΣHCHs = 130 mg kg−1) and control (CS, ΣHCHs = 1.24 mg kg−1) soils and the respective amended soils (APS and ACS). A commercial amendment, coming from organic wastes, was used for soil biostimulation (5% dry weight), and the temporal evolution of the enzymatic activity (dehydrogenase, β-glucosidase activity, phenoloxidase, arylamidase, phosphatase, and urease) and HCHs concentration of the soils was evaluated over 55 days under controlled humidity and temperature conditions. The horse amendment positively influenced the physicochemical properties of the soil by reducing pH (from 8.3 to 8) and increasing the organic matter (TOC from 0.5 to 3.3%) and nutrient content (P and NH4+ from 24.1 to 13.7 to 142.1 and 41.2 mg kg−1, respectively). Consequently, there was a notable enhancement in the soil biological activity, specifically in the enzymatic activity of dehydrogenase, phenol-oxidase, phosphatase, and urease and, therefore, in HCH degradation, which increased from <1 to 75% after the incubation period. According to the chlorine position on the cyclohexane ring, the following ranking has been found for HCHs degradation: β-HCH (46%) < ε-HCH (57%) < α-HCH (91%) ≈ δ-HCH (91%) < γ-HCH (100%). Pentachlorocyclohexene (PCCH) and 1,2,4-trichlorobenzene (1,2,4-TCB) were identified as HCHs degradation metabolites and disappeared at the end of the incubation time. Although further research is required, these preliminary findings suggest that organic amendments represent a sustainable, harmless, and cost-effective biostimulation approach for remediating soils contaminated with recalcitrant HCHs, boosting the circular economy.

More efficient nitrogen utilization through wool pellet and soil inoculation

AbstractIn recent years, many alternative bio‐waste‐based fertilizers have been proven to have a beneficial effect on plant nutrition. These also include wool pellet, whose comprehensive evaluation of synergistic effects on plant development and soil biological activity is still a limited field of investigation. To address this, we explored the potential of combining soil inoculations and N‐rich wool pellet as ecologically sound alternatives to N fertilizers. In a pot experiment, we investigated the effect of Azotobacter vinelandii, the Trichoderma harzianum T34, and wool pellet, individually and in combination, on the growth and nitrate uptake of the test plants, as well as on the biological activity and permanganate‐oxidizable carbon of the sandy soil with low organic matter content. The combination of T. harzianum + wool pellet showed the highest biological activity and permanganate‐oxidizable carbon content, while the untreated control plants exhibited the lowest values. The treatment combination of wool pellet and A. vinelandii exhibited the highest nitrate uptake by plants. Measuring the concentration of photosynthetic pigments, net photosynthesis, transpiration rate, stomatal conductance, and total dry biomass, we found that the treatments led to a significant improvement in the photosynthetic intensity of lettuce plants. These results suggest that wool pellet served as a significant source of N and that their biological activity plays a key role in improving plant parameters. Moreover, the combination of microbial inoculants and wool pellet effectively increases N use efficiency in plant growth.

Effects of canopy gaps on microclimate, soil biological activity and their relationship in a European mixed floodplain forest

Forest canopy gaps can influence understorey microclimate and ecosystem functions such as decomposition. Gaps can arise from silviculture or tree mortality, increasingly influenced by climate change. However, to what degree canopy gaps affect the buffered microclimate in the understorey under macroclimatic changes is unclear. We, therefore, investigated the effect of forest gaps differing in structure and size (25 gaps: single tree gaps up to 0.67 ha cuttings) on microclimate and soil biological activity compared to closed forest in a European mixed floodplain forest. During the investigation period in the drought year 2022 between May and October, mean soil moisture and temperature as well as soil and air temperature fluctuations increased with increasing openness. In summer, the highest difference of monthly means between cuttings and closed forest in the topsoil was 3.98 ± 9.43 % volumetric moisture and 2.05 ± 0.89 °C temperature, and in the air at 30 cm height 0.61 ± 0.35 °C temperature. For buffering, both the over- and understorey tree layers appeared as relevant with a particularly strong influence of understorey density on soil temperature. Three experiments, investigating soil biological activity by quantifying decomposition rates of tea and wooden spatulas as well as mesofauna feeding activity with bait-lamina stripes, revealed no significant differences between gaps and closed forest. However, we found a positive significant effect of mean soil temperature on feeding activity throughout the season. Although soil moisture decreased during this period, it showed no counteracting effect on feeding activity. Generally, very few significant relationships were observed between microclimate and soil biological activity in single experiments. Despite the dry growing season, decomposition rates remained high, suggesting temperature had a stronger influence than soil moisture. We conclude that the microclimatic differences within the gap gradient of our experiment were not strong enough to affect soil biological activity considerably.

Assessment of the biological activity of soil in meadow plant communities comprising ash-leafd maple

Assessment of the biological activity of soil in meadow plant communities comprising ash-leafd maple

Microarthropods improve oat nutritional quality and mediate fertilizer effects on soil biological activity

AbstractSoil biological processes are important drivers of crop productivity in agroecosystems. Soil microarthropods play key roles in nutrient cycling and plant nutrient acquisition, though little is known about how these effects manifest in crop production under different organic fertilizer amendments. We explored the interactive effects of microarthropods and fertilizers on crop productivity in two greenhouse experiments: experiment one involved a single Collembola species, and experiment two involved diverse microarthropod communities. Oats were grown as a model crop in both experiments under one of three initial fauna abundance levels (none, low, and high). In both experiments, four organic fertilization treatments were compared: alfalfa green manure, Kreher's Poultry Litter Compost, Chilean nitrate, and a nonamended control. Oat growth and development were evaluated weekly. During each experiment, 48 pots were selected randomly for destructive harvest at two separate times to mimic forage and grain harvest stages. At each harvest, multiple soil metrics (microarthropods, microbial biomass, microbial enzymes, and soil carbon and nitrogen) and plant metrics (biomass, reproduction, and tissue carbon and nitrogen content) were evaluated. Our findings indicated that microarthropods, both single species and diverse communities, stimulated nitrogen cycling and enhanced crop nutrient status. As microarthropod abundance and diversity increased, microarthropods exerted more effects on soil microbial activity. The effects of the microarthropods enhance the breakdown of fertilizers, ultimately making fertilizer choice less important for soil processes and plant nutrient availability. Our findings suggest that microarthropods drove oat production yields primarily through their effects on soil biological processes.

Valorization of Spent Mushroom Substrate: Establishing the Foundation for Waste-Free Production

Spent mushroom substrate (SMS), often disregarded as waste despite its abundance in organic matter and mineral micronutrients, has emerged as a valuable resource for diverse applications. While Europe and Asia have witnessed extensive research in this field over the past decade, Ukraine’s exploration remains limited. This study conducts a thorough investigation into SMS recycling and reutilization over a 4-year period. Employing experimental and comparative methods, this research unveils compelling insights into the potential of SMS for reintroduction into the primary production cycle and secondary activities. The main conclusions reveal the success of SMS valorization in the production of additives, fertilizers, and alternative fuels. Furthermore, the application of SMS in agroecosystems significantly enhances the soil biological activity. The integration of these methods into production chains not only yields economic benefits for companies but also fosters environmental stewardship, aligning with waste-free practices and the principles of bioeconomy and sustainability.

Rhizophagus intraradices and Azospirillum brasilense improve growth of herbaceous plants and soil biological activity in revegetation of a recovering coal-mining area.

We assessed, in a field experiment, the effects of arbuscular mycorrhizal fungi (Rhizophagus intraradices) and plant growth-promoting bacteria (Azospirillum brasilense) on the soil biological activity and the growth of key pioneer species used in the revegetation of coal-mining areas undergoing recovery. We applied four inoculation treatments to the pioneer plant species (Lablab purpureus, Paspalum notatum, Crotalaria juncea, Neonotonia wightii, Stylosanthes guianensis, Andropogon gayanus and Trifolium repens) used in the recovery process: NI (Control - Non-inoculated), AZO (A. brasilense), AMF (R. intraradices), and co-inoculation of AZO and AMF. On the 75th and 180th days, we measured plant dry mass, mycorrhizal colonization, N and P concentration, and accumulation in plant tissue. We collected soil to quantify glomalin content and soil enzyme activity. After 180 days, we did a phytosociological characterization of the remaining spontaneous plants.The both microorganisms, singly or co-inoculated, promoted increases in different fractions of soil glomalin, acid phosphatase activity, and fluorescein diacetate activity at 75 and 180 days. The inoculation was linked to higher plant biomass production (62-89%) and increased plant P and N accumulation by 34-75% and 70-85% at 180 days, compared with the non-inoculated treatment. Among the pioneer species sown Crotalaria juncea produced the highest biomass at the 75th and 180th days (67% and 76% of all biomass), followed by Lablab purpureus (3% and 0.5%), while the other species failed to establish. At 180 days, we observed twenty spontaneous plant species growing in the area, primarily from the Poaceae family (74%). That suggests that the pioneer species present in the area do not hinder the ecological succession process. Inoculation of R. intraradices and A. brasilense, isolated or combined, increases soil biological activity, growth, and nutrient accumulation in key pioneer plant species, indicating the potential of that technique for the recovery of lands degraded by coal mining.

Assessment of the Combination of Aspergillus niger and Fluorapatite for Cadmium Immobilization in Soil and Its Effect on Soil Biological Properties

ABSTRACT This study evaluated the effects of combining Aspergillus niger and fluorapatite (FAp) as a remediation strategy on cadmium (Cd) immobilization in contaminated soil. Soil Cd speciation, immobilization efficiency, soil microbial abundance, and soil enzyme activities were used as parameters to evaluate the effectiveness of the amendment. European community Bureau of reference (BCR) sequential extraction was used to determine fractions or speciation of the heavy metal in the soil. At 50 mg/kg Cd contamination level, the concentration of acid-soluble species was significantly the lowest at 19.54 mg/kg under the combination amendment. The concentrations (mg/kg) of acid-soluble species under separate applications of FAp and the fungus were 22.05 and 25.34, respectively. At 50 mg/kg contamination, residual fraction or species immobilized under the combination amendment was 5.74 mg/kg, while that immobilized under applications of FAp and the fungus separately were 2.79 mg/kg and 1.46 mg/kg, respectively. At 25 mg/kg contamination level, the residual fraction immobilized under the combination amendment was 8.42 mg/kg, while that of applications of FAp and the fungus separately were 4.28 mg/kg and 1.27 mg/kg, respectively. The higher populations of soil microorganisms and the improved enzyme activities observed under the combination amendment also indicate that combining the fungus with FAp reduced Cd bioavailability and toxicity effectively, and restored soil biological activities.

Problems Encountered in Marketing Sustainable Products and Suggested Solutions in Turkey

The growth of the consumer economy in the last century has led to several significant issues that endanger human life. Overexploitation of nature and soils, the use of pesticides and growth stimulants have had a negative impact on the environment and water resources. The current situation has necessitated that societies worldwide adopt new production approaches to immediately halt soil degradation and improve water quality. One production approach that aims to protect public health and the environment is sustainable production. Sustainable production is an ecological management system that promotes biodiversity, biological cycles, and soil biological activity. The farming system is based on minimal use of off-farm inputs and management practices that restore, maintain or enhance ecological harmony. The aim of this study is to explain the importance of sustainable products and production, to address the problems and suggestions encountered in the marketing of sustainable products in Turkey, and to propose solutions.

Different Tillage and Residue Management Practices Affect Soil Biological Activities and Microbial Culturable Diversity in Rice-Wheat Cropping System Under Reclaimed Sodic Soils

Different Tillage and Residue Management Practices Affect Soil Biological Activities and Microbial Culturable Diversity in Rice-Wheat Cropping System Under Reclaimed Sodic Soils

Sustainable alternative to irrigated maize monoculture in a maize-dominated cropped area: Lessons learned from a system experiment

Maize is the most-produced food crop in the world and is grown in intensive rotations or in monoculture (continuous maize) systems. As maize production has expanded massively across the world, many concerns have emerged about its local environmental and other global impacts. Agronomists have designed innovative cropping systems and assessed them using system experiments to make arable systems more sustainable. However, knowledge is still lacking on the sustainability of innovative cropping systems compared to highly intensive systems such as irrigated maize-based monoculture. Here, we present the assessment results of a nine-year system experiment in Alsace, France, developed to compare an innovative system based on a diversified rotation and innovative management practices (three-year rotation of maize/soybean/winter wheat (plus a cover crop) combined with reduced tillage) with a continuous maize reference system. The results cover a six-year assessment period following an initial three-year design period. Classic criteria, such as profitability, workload, pesticide use, fossil energy consumption and nitrate leaching, were assessed along with other less studied criteria, such as pesticide leaching risk, soil structure, soil chemical quality and soil biological activity. Sustainability – which includes environmental, social and economic dimensions – was assessed with the MASC 2.0 method. Overall sustainability was substantially enhanced in the innovative system (5 out of 7 sustainability classes) in comparison with the low level of the reference system (2 out of 7). This was due to a clear improvement in the environmental performance (from 2 out of 5 to 5 out of 5) while social performance was high in both systems (4 out of 5) and economic performance was low (2 out of 5) due to very low contribution to economic development. Nevertheless, the innovative system had a major drawback: lower profitability, especially when scenarios included high maize prices. Furthermore, herbicide use on maize was higher in the innovative system than in the reference one. Avenues for progress, such as encouraging stakeholder participation at the assessment stage or additional innovations such as multiple cropping, are suggested.

Aggregate composition directly affects the characteristics of soil microbial and nematode communities in a chronosequence of Chinese fir plantations

AbstractMaintaining soil biological diversity is an effective approach in facilitating Chinese fir plantations' sustainable development. However, dynamic characteristics of soil microbial and nematode communities during Chinese fir planting remain unclear, particularly on the aggregate scales, which are the main sites of soil biological activity. Therefore, the research aims to explore how stand age (3, 9, 17, and 26 years) of Chinese fir plantations affects soil microbial biomass (including bacteria, fungi, and actinomycetes, based on the phospholipid fatty acid method), nematode abundances (including bacterivores, fungivores, plant parasites, and omnivores‐predators, based on the modified Baermann funnel method), and their diversity within aggregates of differing sizes (&gt;2, 2–1, 1–0.25, and &lt;0.25 mm). According to the findings, both aggregate size and stand age were major factors affecting soil microbes and nematodes in Chinese fir plantations. Specifically, soil microbial biomass (mainly bacteria), nematode abundances (except fungivores), and their diversity peaked within large macro‐aggregates (&gt;2 mm), and the ranking order for stand ages was 17 &gt; 9 ≈ 3 &gt; 26 years. As the main fractions of soil aggregates in Chinese fir plantations, large macro‐aggregates had suitable internal environments for soil microbial propagation and nematode growth and mainly accumulated at 17 years, which caused the prosperity of soil microbes and nematodes at this stand age. Moreover, the partial least squares path model demonstrated that the influence of stand age on soil microbes and nematodes was indirect; but rather, the aggregate composition played a direct role in shaping their communities. Therefore, in order to maintain soil biological diversity, large macro‐aggregates should be protected during the growth of Chinese fir, especially at the late stage (from 17 to 26 years). Overall, these results could provide valuable insights into Chinese fir plantations' sustainable development.

Biological activity of soil and productivity of spring rape depending on the application of fertilizers in the background of defect application in the forest-steppe conditions of the CCR (Central chernozem region)

Biological activity of soil and productivity of spring rape depending on the application of fertilizers in the background of defect application in the forest-steppe conditions of the CCR (Central chernozem region)

Enzymatic Activity in Different Crop Succession Systems in the Cerrado Region

The enzymatic activity of soil arylsulfatase and β-glucosidase enzymes are biological parameters used to measure the biological activity of soils, an important tool for identifying disturbances in agricultural systems, as they are more sensitive to differentiate changes in soil management when compared to physical and chemical attributes. Thus, the aim was to evaluate arylsulfatase and β-glucosidase levels in different succession systems. Soil samples were collected in agricultural areas with 5 years of experimentation, with four treatments: (1) soybean/corn + brachiaria (Urochloa ruzizienses), (2) soybean/corn, (3) soybean/brachiaria (Urochloa ruzizienses) and (4) soybean/millet. Routine chemical analyses were performed, and the determination of β-glucosidase and arylsulfatase was carried out according to the p-nitrophenyl colorimetric method. As a result, both enzymes presented higher average values in treatment 3. In the biological IQS, treatment 3 differed from treatments 1 and 2. Under the study circumstances, the use of soil with soybean, corn, millet and brachiaria crops provided adequate sustainability conditions, providing high arylsulfatase and β-glucosidase levels.

Analysis and Functional Prediction of Core Bacteria in the Arabidopsis Rhizosphere Microbiome under Drought Stress.

The effects of global warming, population growth, and economic development are increasing the frequency of extreme weather events, such as drought. Among abiotic stresses, drought has the greatest impact on soil biological activity and crop yields. The rhizosphere microbiota, which represents a second gene pool for plants, may help alleviate the effects of drought on crops. In order to investigate the structure and diversity of the bacterial communities on drought stress, this study analyzed the differences in the bacterial communities by high-throughput sequencing and bioinformatical analyses in the rhizosphere of Arabidopsis thaliana under normal and drought conditions. Based on analysis of α and β diversity, the results showed that drought stress had no significant effect on species diversity between groups, but affected species composition. Difference analysis of the treatments showed that the bacteria with positive responses to drought stress were Burkholderia-Caballeronia-Paraburkholderia (BCP) and Streptomyces. Drought stress reduced the complexity of the rhizosphere bacterial co-occurrence network. Streptomyces was at the core of the network in both the control and drought treatments, whereas the enrichment of BCP under drought conditions was likely due to a decrease in competitors. Functional prediction showed that the core bacteria metabolized a wide range of carbohydrates, such as pentose, glycans, and aromatic compounds. Our results provide a scientific and theoretical basis for the use of rhizosphere microbial communities to alleviate plant drought stress and the further exploration of rhizosphere microbial interactions under drought stress.

No tillage and leguminous cover crop improve soil quality in a typical rainfed Mediterranean system

Soil and crop management influence soil organic carbon (SOC), chemical composition, and overall soil quality. In a Mediterranean region, a study initiated in 1994 examined the long-term effects of conventional tillage (CT) versus no-tillage (NT) practices. Initially focusing on continuous durum wheat cultivation until 2009, the experiment later introduced a 2-year rotation of durum wheat and Vicia faba L. cover crops in half of the CT and NT fields. SOC was monitored from 2008 to 2018, while microbial biomass (as dsDNA), soluble nitrogen (N), and enzyme activities (EAs) were monitored from 2011 to 2014 to evaluate the rotation’s impact. Between 2009 and 2018, CT yields were on average 15% higher than NT, especially during high rainfall years. NT significantly increased SOC content in the 0–30 cm soil layer, along with higher levels of soluble N, dsDNA, and EAs at 0–10 cm depth. NT led to a 23% and 10% increase in SOC stock and SOC stock per equivalent soil mass compared to CT. EAs increased by over 50% under NT, indicating enhanced biological activity. The SOC increase due to NT was limited to the top 10 cm, with a decrease at deeper depths (up to 50 cm). Introducing cover crops over 4 years did not yield significant impacts, suggesting the need for a longer period to observe noticeable effects. Overall, adopting NT practices resulted in higher SOC concentration, enhanced soil biological activity, and improved biogeochemical cycles, emphasizing the positive impact of NT on soil health and sustainability.

Comprehensive bioremediation effect of phosphorus-mineralized bacterium Enterobacter sp. PMB-5 on cadmium contaminated soil-crop system

Phosphate-mineralizing bacteria (PMBs) have been widely studied by inducing phosphate heavy metal precipitation, but current researches neglect to study their effects on soil-microbe-crop systems on cadmium (Cd) contaminated. Based on this, a strain PMB, Enterobacter sp. PMB-5, was inoculated into Cd contaminated pots to detect soil characteristics, Cd occurrence forms, soil biological activities, plant physiological and biochemical indicators. The results showed that the inoculation of strain PMB-5 significantly increased the available phosphorus content (85.97%−138.64%), Cd-residual fraction (11.04%−29.73%), soil enzyme activities (31.94%−304.63%), plant biomass (6.10%−59.81%), while decreased the state of Cd-HOAc (11.50%−31.17%) and plant bioconcentration factor (23.76%−44.24%). These findings indicated that strain PMB-5 could perform the function of phosphorus solubilization to realize the immobilization of Cd in the complex soil environment. Moreover, SEM-EDS, FTIR, XPS, and XRD analysis revealed that strain PMB-5 does not significantly alter the soil morphology, structure, elemental distribution, and chemical composition, which suggested that remediation of Cd contamination using strain PMB-5 would not further burden the soil. This research implies that PMB-5 could be a safe and effective bioinoculant for remediating Cd-contaminated soils, contributing to the sustainable management of soil health in contaminated environments.

WITHDRAWN: The evaluation model for factors affecting soil quality in waste dumps open-pit coal waste dumps based on fuzzy mathematics

Taking the Hequ open-pit coal mine waste dump as the research object, using the fuzzy analytic hierarchy process and expert scoring theory, 4 criteria and 25 factors were established, including the evaluation model of soil structure stability, soil water retention capacity, soil fertilizer retention capacity, soil biological activity, and factors affecting soil quality in the waste dumps. Using YAAHP calculation software, the weights of various influencing factors on the soil fertility restoration ability of the waste dumps were determined. Combining a fuzzy comprehensive evaluation method to determine soil nutrient standards, soil quality evaluation is divided into 5 levels, and the main influencing factors of soil restoration ability in open-pit coal mine waste disposal sites are quantitatively analyzed. The results showed that: The weight of soil fertility in the criterion layer of the evaluation model was the largest (0.548), followed by soil water retention capacity (0.198), soil biological activity (0.140), and soil structural stability (0.114). The weight of organic matter F1 in the factor layer was the highest (0.116). Due to the final evaluation score of the soil recovery ability of the target layer open-pit coal mine waste dumps is 53.584, the soil quality and nutrients of the waste dumps are in the middle level. The main factors of soil resilience are soil fertility and soil organic matter content, to provide management strategies for improving soil quality of dump, accelerating soil reconstruction, and promoting soil ecosystem restoration.

THE EFFECT OF ZINC ON THE ACTIVITY OF THE ENZYME DEHYDROGENASE IN SIEROZEM

In this research paper, the role of zinc as a highly toxic element in the soil–plant system and the degree of influence on the enzymatic activity of dehydrogenase in serozem were studied. Experimental work was carried out using the box method, and various concentrations of zinc were introduced into the soil artificially. Research has established that soil contamination with zinc in the amount of 0,5; 2,5; 5,0 MPC acts as an inhibitor of redox reactions characterizing soil fertility and biological activity. As a result of the conducted model experiments, it was found that soil contamination with zinc reduces the enzymatic activity of dehydrogenase. Zinc binds to the sulfhydryl groups of the dehydrogenase molecule, thereby disrupting its enzymatic properties. There is a tendency to decrease the activity of dehydrogenase with an increase in the zinc content in the soil. At 0,5; 2,5; 5,0 MPC of zinc in the soil, the indicators of dehydrogenase activity decrease by 77.6-93.0% compared with the control experiment. In all samples, it was found that the introduction of vermicompost into the soil slightly increases the dehydrogenase activity compared to the control experiment, despite the addition of zinc in various concentrations. This can be explained by an increase in the number of microorganisms that contribute to the synthesis of enzymes when adding vermicompost to the soil. Vermicompost not only increases the activity of enzymes in the soil, but also increases soil fertility.