Background: One of the most popular sports in the world is Football. One of the most usual injury from Hamstring Tightness is hamstring muscle damage. Football is therefore a dynamic sports that causes more injuries than other sports. In clinical and fitness contexts, the term is frequently used to represent restricted motion based on by adaptive soft tissue shortening, mainly minimal muscular shortening. The term “myofascial trigger point” refers to a hyperirritable point in skeletal muscle that is connected to hypersensitive palpable nodules in a taut band and has the characteristics of localized pain, soreness, motor dysfunction, and autonomics disturbances. Dry Needling is trained technique that involves penetrating the skin with a small needle to stimulate trigger points, muscles and connective tissue for the treatment of musculoskeletal pain. Method: Football players with Hamstring tightness [n=53] were included in the study according to the selection criteria. Subjects were evaluated for hamstring tightness using popliteal angle and kicking speed was assessed before and after the treatment. Subjects were given dry needling on hamstring muscle for the period of 1 week alternately. Results: Statistical analysis was done using paired t test. Based on the result there is increased in popliteal angle p=[0.000] and kicking speed time p=[0.0001] of the players when compared with pre and post treatment. Dry needling technique was proved statistically in reducing hamstring muscle tightness. Conclusion: It is concluded that Dry needling proves to be effective in improving hamstring flexibility and kicking speed among football players and hence helps in bringing out their best performance on the field.