The purpose of this study is to investigate the effect of applying instrument-assisted soft tissue mobilization (IASTM) to muscle fibrosis due to motor-induced muscle damage. 24 male students participated in this study, and were randomly assigned to the IASTM group and the control group, respectively. For the IASTM group, IASTM was applied to muscles such as rectal femur muscles 48 hours after exercise. Maximum strength (leg extension and leg refraction), TGF-γ, CK, LDH, TNF-,, and IL-6 were measured as variables, and repeated measurement variance analysis was used for statistical analysis. As a result of the study, there was: Significant interaction effects of time and groups on maximum strength (leg dilatation and leg flexion), TGF-γ, CK, LDH, TNF-및, and IL-6 and IASTM groups showed more improvements in recovery at maximum strength (leg dilatation and leg flexion), TGF-γ, CK, LDH, TNF-α, and IL-6. In conclusion, the application of IASTM to male students showed that it was effective in improving muscle fibrosis due to muscle damage caused by exercise.