Soft Lines Research Articles

The Effect of Microwave Irradiation and Some Denture Cleansers on The Bond Strength of Soft Liner to CAD/CAM-Milled Acrylic Resin

Disinfection of dentures and denture liners was desired for cross-contamination prevention and improved safety. Nonetheless, it has been discovered that these procedures impact the physical and mechanical features of denture liners.To assess the impact of different disinfection procedures on the tensile bond strength of an acrylic-based soft liner with CAD/CAM-based acrylic denture resin. Material and Methods: 25 specimens were collected and randomly divided into five groups (sodium hypochlorite, chlorohexidine, cleansing tablet, microwave, and control groups), following the daily disinfection protocol, the samples were stored at room temperature (23±2°C) in distilled water. After 30 days, the tensile bond strength was determined using universal testing equipment (Gester, China). The specimens were subjected to force by the machine at a steady crosshead speed of 5 mm/minute till the failure was recorded. The data were analyzed using statistics software (SPSS). Results: Showed that the control group had a higher mean value for bond strength, whereas the chlorhexidine group had a lower value. There was no significant difference between the control and cleansing tablet groups. However, they were significantly different from the microwave, CHX, and NaOCl groups. The NaOCl soft liner group is significantly different from all the other groups. No significant difference is found between microwave and CHX disinfected liner groups. Conclusion: The tensile bond strength of all disinfected soft liners decreased as compared with the control, which was not significant with the protefix tab but significant with the NaOCl, CHX, and microwave groups.

The Effect of Various Disinfection Methods on The Tensile Bond Strength of Soft Lining Material

Disinfection of dentures and resilient denture liners were preferred for cross-contamination treatment and better safety. Nevertheless, it has been found that these techniques affect the physical and mechanical properties of denture liners. Objective: To evaluate the impact of various disinfection methods on the tensile bond strength of acrylic soft liner. Material and Methods: 25 samples were made, then divided randomly into five groups (4 disinfection groups as well as a control group), after the daily disinfection procedure, distilled water was used to store the samples at room temperature. After 30 days, a universal testing device (Gester, China) was used to assess the tens ile bond strength. The machine applied force to the specim ens at a crosshead constant speed of 5 mm/minute until failure was recorded. Statistics software (SPSS) was used to analyze the measurements. Results: After 30 days of regular ex posure to different disinfection methods, a greater mean value for bond strength showed in the control group while the lesse r value was in the chlorhexidine group. No significant difference was found between the control and Protefix tablet groups. The sodium hypochlorite and microwave groups had no significant difference between them, but they were significantly different from the control, Protefix tablet, and chlorhexidine groups. the chlorhexidine group was significantly different from the control and other disinfected groups. Conclusion: All disinfected soft liners revealed a decrease i n tensile bond strength, which was not significant with the protefix cleansing tablet but significant with the chlorhexidine, sodium hypochlorite, and microwave regimes.

Phenotyping and predicting wheat spike characteristics using image analysis and machine learning

AbstractImprovements in trait phenotyping are needed to increase the quantity and quality of data available for genetic improvement of crops. In this study, we used moderate throughput image analysis and machine learning as a pipeline for phenotyping a key wheat spike characteristic: spikelet number per spike. A population of 594 soft red winter wheat inbred lines was evaluated in the field for 2 years and images of wheat spikes were taken and used to train deep‐learning algorithms to predict spikelet number. A total of 12,717 images were used to train, test, and validate a basic regression convolutional neural network (CNN), a visual geometry group application regression model, VGG16, the ResNet152V2 model, and the EfficientNetV2L model. The EfficientNetV2L model was the most accurate, having the lowest mean absolute error, second lowest root mean square error, and highest coefficient of determination (mean absolute error [MAE] = 0.60, root mean square error [RMSE] = 0.79, and R2 = 0.90). The ResNet152V2 model was slightly less accurate with a slightly better fit (MAE = 0.61,m RMSE = 0.78, and R2 = 0.87), followed by the basic CNN (MAE = 0.75, RMSE = 1.00, and R2 = 0.74) and finally by the VGG16 (MAE = 1.51, RMSE = 1.29, and R2 = 0.076). With an average error of just above one half of a spikelet, utilizing image analysis and machine learning counting methods could be used for multiple breeding applications, including direct selection of spikelet number, to provide data to identify quantitative trait loci, or for training whole genome selection models.

Comparative Evaluation of Tensile Bond Strength of Two Soft Liners Bonded to Heat-Cured and 3D-Printed Denture Base Materials

Comparative Evaluation of Tensile Bond Strength of Two Soft Liners Bonded to Heat-Cured and 3D-Printed Denture Base Materials

Comparative Evaluation of Hardness and Energy Absorption of Some Commercially Available Chairside Silicone-Based Soft Denture Liners and a Heat-Cured Soft Denture Liner.

To investigate the hardness and energy absorption of four commercially available chairside types of silicone materials and compare their properties with heat-cured silicone material. The chairside materials investigated were GC reline soft, mucopren soft, sofreliner soft and elite soft relining. The heat-cured polymer silicone material was Molloplast B. All soft lining materials were processed according to manufacturers' instructions. Two properties were investigated. Ten specimens for each test were prepared for each soft liner except for the water absorption and solubility test, for which only five specimens were prepared. The specimens of energy absorption (10 × 10 × 3 mm) were tested using a Lloyd instruments testing machine. Hardness specimens (38 × 38 × 3) were tested using a shore A durometer and were divided into two subgroups; dry and wet storage. The specimens of energy absorption (10 × 10 × 3 mm) were tested using a Lloyd instruments testing machine. Sofreliner soft was significantly softer than Molloplast B. GC reline soft was significantly harder than molloplast B. At high loads, sofreliner soft and elite soft relining was significantly more resilient than molloplast B. Mucopren soft was significantly stiffer than Molloplast B. At low loads, all materials showed similarities in stiffness and resilience; the difference between them was insignificant. After one month of immersion, GC reline and mucopren significantly increased hardness values. In all conditions and at all four-time points, the hardness values for GC Reline soft were the greatest, and hardness values for Sofreliner Soft were the least. Some chairside soft denture lining materials could have similar significant properties to molloplast-B, such as sofreliner soft and elite.

Impact of apigenin and seashell nano-additives on the antifungal and roughness behavior of a soft denture liner.

Fungal colonization of the soft denture liner is the first step in the development of denture-induced stomatitis. The study aims to assess apigenin and seashell nano-additives for their antifungal efficacy and their impact on the surface roughness of a soft denture liner. The study was accomplished in the Colleges of Dentistry in Duhok, Mosul and Hawler Medical Universities. The Antifungal efficacy against Candida albicans was performed by the minimum inhibition concentration (MIC), for apigenin the MIC was determined by agar well diffusion and set at (0.25%, 0.5% and 1%) while for seashells, MIC was determined by broth dilution and set at (1.25%, 2.5% and 5%). Fungal adhesion was conducted on seven groups (unmodified soft liner and six groups of the modified liner with the antifungal concentrations (three for each nanoparticle). A total of forty-nine square-shaped specimens (10*10*2mm) of (GC, Super-soft, heat-cured, USA) soft liner were prepared, the adherent fungal cells were enumerated under a light microscope for each specimen in four fields and the results were expressed as fungal cells/mm2. For the surface roughness, forty-nine specimens of (20*10*3 mm) of the soft liner were prepared and the average surface roughness was obtained in µm using a profilometer (Talysurf, Taylor Hobson, UK). Apigenin and seashell-modified soft liner observed a significant decrease in both fungal adhesion and surface roughness compared to the unmodified liner and the reduction was related directly to the concentration of both additives. Apigenin and seashell nano-additives were effective as antifungal agents beside improving the surface roughness of the soft liner.

Effect Of Montmorillonite Incorporation on Shear Bond Strength And Surface Hardness Of Acrylic Base Soft Lining Material (In Vitro Study)

Abstract Objective: One problem with the soft lining material is that it detaches from the acrylic base of dentures after a particular time period. So, the aim of this work was to evaluate how adding Montmorillonite nano clay would affect the soft lining material's shear bond strength and surface hardness. Materials and Methods: The heat-cured acrylic denture material for the control group, polymer reinforced with (0.25%,0.5% by wt.) of montmorillonite nanoparticles for experimental groups was used in this study. For the shear bond strength test 60 blocks of acrylic-based material were constructed, each pair connected with soft lining material, the other 30 specimens for the surface hardness tests were carried out. to be tested with a shore A durometer. The data were statistically examined using the One-Way ANOVA test and Bonferroni multiple comparisons test. Result: 0.5% by weight MMTNPS should significantly increase in both shear bonding strength and surface hardness . Conclusion: MMTNPs were remarkably successful in improving the the strength of the shear connection between acrylic and soft liners.

Comparative Analysis of Properties in Chairside Silicone Denture Liners versus Heat-Cured Molloplast-B: An In-Depth In Vitro Evaluation.

BACKGROUND We investigated the bond strength, tensile strength, and water absorption properties of 4 widely used chairside silicone long-term denture soft lining materials and compared properties with those of heat-cured silicone material. MATERIAL AND METHODS Four chairside materials (GC Reline Soft, Mucopren Soft, Sofreliner Soft, and Elite Soft Relining) and a heat-cured silicone material (Molloplast B) were investigated. For tensile and shear bond strength and tensile strength, samples were prepared according to the manufacturers' instructions and the testing machine used. For water absorption, IOS 1567 was followed to prepare specimens. Bond strengths were measured using a Lloyd Instruments materials testing machine. Ten specimens for each test were prepared for each soft liner, except for water absorption and solubility tests, for which only 5 specimens were prepared. ANOVA, Bonferroni, and Kruskal-Wallis tests were used to compare values of materials and assess changes over time. RESULTS There was strong evidence of differences in the properties between materials. GC Reline Soft showed comparable tensile bond (1.4±0.6 MPa), shear bond (1.1±0.4 MPa), tensile strength (5.44±0.98), water absorption (0.92±0.2 µg/mm³), and solubility values (0.3±0.2 µg/mm3) to that of Molloplast B (1.4±0.4; 1.6±0.5; 4.53±0.9 MPa, 1.7±0.3, and 1.0±0.3 µg/mm³, respectively). The water solubility of Sofreliner Soft was significantly lower (1.5±0.8 µg/mm³) than that of Molloplast B. CONCLUSIONS There were significant differences between some properties of 4 chairside denture soft lining materials and that of Molloplast B, which had higher shear and tensile bond strength than chairside denture silicone soft lining materials but had the highest water solubility.

In vitro comparative assessment of the denture cleansers effectiveness on the surface hardness of permanent silicone denture line

The purpose of the current research was to evaluate the impact of various denture cleaners on the surface hardness of permanent silicone denture lines. In the present study, 124 specimens were included comprising of soft liners made of permanent silicone, which were made from the 15 mm x 10 mm custom metal mold dimensions, where 62 samples were made of Molossil™ and 62 other of Voco™ which are commercially available liners for permanent dentures. These samples were then divided into 4 groups of equal specimen numbers (n=32) depending on the cleansing treatments. Group I specimens were cleaned with water daily, and Group II Polident™ Group III was given SecureTM denture cleaning solution, while Group IV was given PeriogardTM denture cleaning solution. The samples were assessed at 1st week, 1st month, and 6th month after cleanser use. The study results showed that at the 6th-month follow-up after the use of denture cleansers, all of the samples had considerably increased surface hardness compared to the baseline. Statistically significant results were seen with Periogard™, Secure™, and Polident™ compared to Group I controls. In Voco™ denture liners, minor changes were seen compared to Molossil™ soft liners with time in all the groups. The results of this investigation are significantly well cleansing action is seen with Voco™ soft denture lining material compared to Mollosil™ soft denture lining material, and surface hardness is least affected by Polident™ denture cleanser compared to Secure™ and Periogard™ denture cleansing agents.

Quantitative proteomic analysis of super soft kernel texture in soft white spring wheat.

Super soft kernel texture is associated with superior milling and baking performance in soft wheat. To understand the mechanism underlying super soft kernel texture, we studied proteomic changes between a normal soft and a super soft during kernel development. The cultivar 'Alpowa', a soft white spring wheat, was crossed to a closely related super soft spring wheat line 'BC2SS163' to produce F6 recombinant inbred lines (RILs). Four normal soft RILs and four super soft RILs along with the parents were selected for proteomic analysis. Alpowa and the normal soft RILs showed hardness indices of 20 to 30, whereas BC2SS163 and the super soft RILs showed hardness indices of -2 to -6. Kernels were collected from normal soft and super soft genotypes at 7 days post anthesis (dpa), 14 dpa, 28 dpa, and maturity and were subject to quantitative proteomic analysis. Throughout kernel development, 175 differentially abundant proteins (DAPs) were identified. Most DAPs were observed at 7 dpa, 14 dpa, and 28 dpa. Of the 175 DAPs, 32 had higher abundance in normal soft wheat, whereas 143 DAPs had higher abundance in super soft wheat. A total of 18 DAPs were associated with carbohydrate metabolism and five DAPs were associated with lipids. The gene TraesCS4B02G091100.1 on chromosome arm 4BS, which encodes for sucrose-phosphate synthase, was identified as a candidate gene for super soft kernel texture in BC2SS163. This study enhanced our understanding of the mechanism underlying super soft kernel texture in soft white spring wheat.

Non-surgical periodontal treatment for black triangle

Objective: This case report aims to introduce the black triangle therapy in Miller’s Class IV gingival recession. Methods: The patient complained of spacing between the upper and lower teeth causing food accumulation, “long teeth”, and receding gums which were unaesthetic during speaking. Clinical examination of the maxillary and mandibular anterior revealed Miller Class IV gingival recession, large black triangles, no tooth mobility nor pocket probing, and no gingival inflammation signs. Based on clinical and radiograph examination, nonsurgical therapy using gingival mask was the best option. Nonsurgical therapy using gingival mask was made using indirect technique with an elastomeric impression. An extra-soft soft liner was chosen for gingival mask materials. Results: The black triangle was covered. The “long teeth” appearance and hypersensitivity were reduced. Conclusion: Gingival masks is a good and predictable nonsurgical treatment option for treating black triangle in Miller Class IV gingival recession.

Scattering of plane compressional waves by a variable thickness circular lined tunnel

The determination of the dynamic stress concentration factor (DSCF) associated to a circular lined tunnel with variable thickness liner incident upon by compressional P-waves is of particular interest. Due to the lack of symmetry, the treatment of such a problem via the traditional wave-function expansion method is not possible. To circumvent this dilemma it is proposed to employ the dynamic equivalent inclusion method (DEIM). As it will be shown, this method which is based on the notions of the eigenstress and eigenbody-force fields provides an accurate solution for the problem under consideration. In the simple special case where a tunnel with a concentric liner is subjected to plane compressional P-waves the exact wave-function expansion solution given in the literature is recovered. The effect of liner thickness variability on the static and dynamic stress concentration along the interior surface of the liner and along the liner-matrix interface is studied in detail through numerical examples. The maximum value of the DSCF for the concrete and steel liners with positive and negative values of eccentricity is obtained and compared with those of the corresponding concentric cases. It is observed that the eccentricity of the liner results in different behaviors of the stress concentration for the hard (steel) and soft (concrete) liners.

Prosthetic Management of Flap-Related Complications Following Glossectomy in Locally Advanced Tongue Cancer.

Total glossectomy defects resulting from postoncologic resection are commonly reconstructed with locoregional or free flaps. However, effectively managing complications that may arise after reconstruction can be a significant challenge. We present a unique case series describing prosthetic management of flap-related complications following glossectomy in patients treated for locally advanced tongue cancer. Three patients underwent total glossectomy, neck dissection, reconstruction using free flap, and tracheostomy. Two patients developed an intraoral fistula located in the anterior region of the floor of the mouth. The third patient developed a flap failure requiring a second procedure using a pectoralis major myocutaneous flap for correction of the orocutaneous fistula that ultimately did not heal. Mandibular obturator prostheses lined with a soft liner were fabricated for all the patients, which helped reduce salivary incontinence and improve swallowing and speech. This case series highlights that a collaborative interdisciplinary team approach is crucial for optimizing postoperative function and outcomes when managing complications from reconstructive procedures.

Candida albicans adherence on soft denture liner coated with silica and titanium nanoparticles

Candida albicans adherence on soft denture liner coated with silica and titanium nanoparticles

X-ray metal line emission from the hot circumgalactic medium: probing the effects of supermassive black hole feedback

ABSTRACT We derive predictions from state-of-the-art cosmological galaxy simulations for the spatial distribution of the hot circumgalactic medium (CGM, [0.1–1]R200c) through its emission lines in the X-ray soft band ([0.3–1.3] keV). In particular, we compare IllustrisTNG, EAGLE, and SIMBA and focus on galaxies with stellar mass $10^{10-11.6}\, \rm {M}_{\odot }$ at z = 0. The three simulation models return significantly different surface brightness radial profiles of prominent emission lines from ionized metals such as O vii(f), O viii, and Fe xvii as a function of galaxy mass. Likewise, the three simulations predict varying azimuthal distributions of line emission with respect to the galactic stellar planes, with IllustrisTNG predicting the strongest angular modulation of CGM physical properties at radial range ${{\gtrsim}0.3{-}0.5\, R_{200c}}$. This anisotropic signal is more prominent for higher energy lines, where it can manifest as X-ray eROSITA-like bubbles. Despite different models of stellar and supermassive black hole (SMBH) feedback, the three simulations consistently predict a dichotomy between star-forming and quiescent galaxies at the Milky Way and Andromeda mass range, where the former are X-ray brighter than the latter. This is a signature of SMBH-driven outflows, which are responsible for quenching star formation. Finally, we explore the prospect of testing these predictions with a microcalorimeter-based X-ray mission concept with a large field of view. Such a mission would probe the extended hot CGM via soft X-ray line emission, determine the physical properties of the CGM, including temperature, from the measurement of line ratios, and provide critical constraints on the efficiency and impact of SMBH feedback on the CGM.

Effect of Ionic Liquids on Mechanical, Physical, and Antifungal Properties and Biocompatibility of a Soft Denture Lining Material.

This study aims to evaluate the effect of ionic liquids and their structure on the mechanical (tensile bond strength (TBS) and Shore A hardness), mass change, and antifungal properties of soft denture lining material. Butyl pyridinium chloride (BPCL) and octyl pyridinium chloride (OPCL) were synthesized, characterized, and mixed in concentrations ranging from 0.65-10% w/w with a soft denture liner (Molloplast-B) and were divided into seven groups (C, BPCL1-3, and OPCL1-3). The TBS of bar-shaped specimens was calculated on a Universal Testing Machine. For Shore A hardness, disc-shaped specimens were analyzed using a durometer. The mass change (%) of specimens was calculated by the weight loss method. The antifungal potential of ionic liquids and test specimens was measured using agar well and disc diffusion methods (p ≤ 0.05). The alamarBlue assay was performed to assess the biocompatibility of the samples. The mean TBS values of Molloplast-B samples were significantly lower (p ≤ 0.05) for all groups except for OPCL1. Compared with the control, the mean shore A hardness values were significantly higher (p ≤ 0.05) for samples in groups BPCL 2 and 3. After 6 weeks, the OPCL samples showed a significantly lower (p ≤ 0.05) mass change as compared to the control. Agar well diffusion methods demonstrated a maximum zone of inhibition for 2.5% OPCL (20.5 ± 0.05 mm) after 24 h. Disc diffusion methods showed no zones of inhibition. The biocompatibility of the ionic liquid-modified sample was comparable to that of the control. The addition of ionic liquids in Molloplast-B improved the liner's surface texture, increased its hardness, and decreased its % mass change and tensile strength. Ionic liquids exhibited potent antifungal activity.

Cephalometric Assessment of Differences in Soft Tissue and Dental Compensation in Skeletal Class II Div 1 Malocclusion.

This study was conducted to evaluate the differences in soft tissue as well as dental compensation in skeletal Class II div 1 malocclusion. The study sample consists of pre-treatment lateral cephalograms of 100 subjects of both gender having skeletal Class II and dental Class II div 1 malocclusion visiting the Department of Orthodontics and Dentofacial Orthopaedics, Bharati Vidyapeeth Dental College, and Hospital Navi Mumbai. Cephalograms of the subjects fulfilling the inclusion criteria were included in this study. This research was conducted to assess dental and soft tissue compensation in skeletal Class II div 1 malocclusion and comparative assessment of lip prominence measured from two soft tissue vertical reference lines. The 100 subjects selected randomly out of which 44 men and 56 women, age ranging from 18 to 35 years. ARNETT'S method and PROFFIT'S method showed statistically significant as P < 0.0001. PROFFIT'S method and LEGAN-BURSTONE'S method showed statistically significant as P < 0.0001.

Effect of Sandblasting Surface Treatment on the Bond Strength and Surface Roughness Between the 3D-Printed Denture Base and Silicon-Based Soft Liner.

Effect of Sandblasting Surface Treatment on the Bond Strength and Surface Roughness Between the 3D-Printed Denture Base and Silicon-Based Soft Liner.

Tensile Bond Strength between Different Denture Base Materials and Soft Denture Liners.

(1) Background: Various materials are available for CAD-CAM denture base fabrication, for both additive and subtractive manufacturing. However, little has been reported on bond strength to soft denture liners. Therefore, the aim of this study was to investigate tensile bond strength, comparing between different denture base materials and soft denture liners. (2) Methods: Seven different materials were used for denture base fabrication: one heat-polymerized polymethyl methacrylate, three materials for subtractive manufacturing, two materials for additive manufacturing and one polyamide. Two materials were used for soft denture lining: one silicone-based and one acrylate-based. The study was conducted according to the specification ISO No. 10139-2:2016, and the type of failure was determined. The Kruskal-Wallis test with Dunn's post hoc test was used to analyse the values of tensile bond strength, and Fisher's exact test was used to analyse the type of failure. p Values < 0.05 were considered statistically significant. (3) Results: The tensile bond strength values were not statistically significantly different combining all the materials used for denture base fabrication with the acrylate-based soft denture liner (p > 0.05), and the average values ranged between 0.19 and 0.25 Mpa. The tensile bond strength values of the different denture base materials and silicone-based denture liner were statistically significantly different (p < 0.05), and the average values ranged between 1.49 and 3.07 Mpa. The type of failure was predominantly adhesive between polyamide and both additive-manufactured denture base materials in combination with the acrylate-based soft liner (p < 0.05). (4) Conclusions: The use of digital technologies in denture base fabrication can have an influence on different tensile bond strength values for soft denture liners, with different types of failure when compared with heat-cured PMMA. Similar tensile bond strength values were found between the acrylate-based soft denture liner and denture base materials. Significant differences in tensile bond strength values were found between the silicone-based soft denture liner and denture base materials, where the additive-manufactured and polyamide denture base materials showed lower values than heat-cured PMMA and subtractive-manufactured denture base materials.

Phytohormonal balance in leaves of the soft wheat lines isogenic for VRN genes

The paper presents results of the study of the phytohormonal balance in mature, formed leaves of the lines of common wheat (Triticum aestivum L.) of the winter variety Olvia during the transition from vegetative to generative development. Near isogenic for the VRN genes lines (NILs) created in the gene pool of the Olvia variety and plants of the vernalized and non-vernalized winter variety were used. The experiments were carried out under the conditions of a vegetation experiment in the factorial chamber of the Department of Physiology and Biochemistry of Plants and Microorganisms of the V.N. Karazin KhNU. During the experiment, we conducted physiological observations and analyzed the development rate of experimental plants. Phytohormone analysis was carried out in fixed plant material by a chromatographic distribution of phytohormone mixture with thin-layer chromatography. The phytohormones were identified by the reference standards irradiating the chromatograms with ultraviolet UV (254 nm), and the content was determined by biotesting methods. The level of main classes of classical growth-stimulating phytohormones (auxins (IAA), cytokinins (CK), and gibberellins (GA), and growth-inhibiting hormones (abscisins (ABA)) was analyzed. The indicators of phytohormonal balance were calculated as the ratio of growth-stimulating and growth-inhibiting hormones. The results of the experiments showed that phytohormones in mature, formed leaves of the experimental plants are represented by auxins – 64.9-70.7 μg/g, cytokinins – 26.6-30.5 μg/g, gibberellins – 179.47-228.68 μg/g, and abscisins – 54.06-89.76 μg/g of dry weight. Among the phytohormone classes studied, the minimal was the cytokinins’ content, while the phytohormones of terpenoid nature (gibberellins and abscisins) were represented best. It has been established that the phytohormonal balance viz. the ratio of growth-stimulating and growth-inhibiting phytohormones reflects the development rate of experimental plants. Rapidly developing plants of isolines VRN 1 and VRN 3, and the plants of vernalized variety were characterized by the maximum phytohormone balance (especially GA/ABA), while the slowly developing plants of the isoline VRN 2 and the non-vernalized variety Olvia had the minimum balance. Since the plant organism is an integrated system of organs and functions, we assume that this indicator – phytohormonal balance in plant leaves, can be used as a marker of the ontogenetic state of the entire plant organism. The identified changes in the phytohormonal status of mature, formed leaves and the development rates of experimental plants have the same regularities in all the models used in our research: the model of isogenic lines and the model of vernalized and non-vernalized plants of the winter variety. This fact makes it possible to assume that changes in the phytohormone balance of mature leaves, which reflect the ontogenetic state of the entire plant organism, are determined by the genotypic and phenotypic (epigenetic) influence.