Soft Laser Research Articles

Photobiomodulation by soft laser irradiation with and without ibuprofen improves success rate of inferior alveolar nerve block using 2% lignocaine with adrenaline in symptomatic irreversible pulpitis of mandibular molar teeth: a double-blind, randomized placebo-controlled trial.

Achieving successful pain control and adequate anesthesia through an inferior alveolar nerve block for endodontic treatment in cases with symptomatic irreversible pulpitis (SIP) is difficult, especially in mandibular molars. This study was designed to compare the effect of oral medication with ibuprofen and soft laser therapy on inferior alveolar nerve block during endodontic treatment. The trial comprised 180 patients (45 each group) with SIP. Four groups of patients were created: group 1 received 400 mg of ibuprofen; group 2 received soft laser irradiation; group 3 received a combination of soft laser and ibuprofen 400 mg; and group 4 received a placebo 1 h prior to local anesthesia. Patients recorded their pain scores on the Heft-Parker visual analog scale (VAS) before the start of intervention, 15 min after anesthesia, during access cavity preparation, and ultimately during root canal instrumentation. Each patient also rated their level of discomfort on a VAS. Every stage with no or minimal discomfort was deemed successful. The chi-square, Kruskal-Wallis, and one-way analysis of variance tests were used to evaluate the data. The best success rate was achieved for soft laser ibuprofen combination, ibuprofen and soft laser groups reported similar success results, and control group recorded the least pain scores. The mean pain scores were lowest for group 3 and highest for group 4 (P < 0.001). Ibuprofen and soft laser combination was significantly better than control group (P < 0.001). There was no significant difference between ibuprofen and laser groups (P = 0.24). For teeth with irreversible pulpitis, preoperative ibuprofen treatment combined with soft laser irradiation greatly improved the success rates of inferior alveolar nerve block anesthesia.

The effectiveness of two types of low-level laser therapy in patients with persistent tinnitus.

Tinnitus is a perception disorder of sound with no hearing impulse. It is a very common otology complaint that leads to worsening quality of life. The experience of sound is only the product of neural system activity, with no matching mechanical or vibratory activity in the cochlea, and is unrelated to any external stimuli. Low-level laser therapy (LLLT) is a medical treatment of tinnitus that uses low-energy-level lasers or light-emitting diodes to stimulate or inhibit cellular function. The study included nine patients aged 20-68 years with unilateral or bilateral tinnitus. It was a self-controlled clinical trial study on subjective tinnitus. All patients attended the ENT outpatient Department, Rzgari Teaching Hospital, Erbil, Iraq. Two types of low-level laser therapy (LLLT) devices were used for patients. The first tool, a soft laser called a Tinnitool, has a wavelength of 660 nm and a power of 100 mW. The second tool is a Tinnitus Pen, which has a wavelength of 650 nm and a power of 5 mW. Seven females (77.7%) and two males (22.2%) participated in this study during one month. The mean age of the study sample was 44 years, with a standard deviation of 15.59 years. There was a significant improvement in the comparison of both types of therapy low-level laser before and after treatment, which reduced the tinnitus level among patients from 70% before treatment to 59% and 65.50%, respectively, after one month of treatment. A paired t test was applied to assess this difference before and after treatment. LLLT devices can be an effective device tool for the treatment of tinnitus and can reduce the symptoms of annoyance that affect the life of the sufferer.

PENGARUH PEMAPARAN LASERPUNKTUR PADA TINGKAT (KEBUNTINGAN) KETEBALAN PERUT DAN FERTILISASI IKAN TAWES (PUNTIUS JAVANICUS)

Tawes fish (Puntius javanicus) is a freshwater fish that is easy to cultivate but tawes fish can spawn naturally only during the rainy season. Meanwhile, the market demand for tawes is increasing and the availability of seeds is not enough to meet market demand. To overcome this problem, it is necessary to apply technological advances in the form of a biostimulator for the development of Tawes fish farming business. The biostimulator used is a laser. Several studies state that the application of the use of soft lasers can be used to accelerate gonadal maturation. The purpose of this study was to determine the level of fertility and fertilization of tawes fish (Puntius javanicus), as well as to determine the level of abdominal thickness after laserpuncture exposure to prospective female parents. The method used in this study was an experimental method with a completely randomized design. This study consisted of 5 treatments with 1 control with each treatment being repeated 3 times, laserpuncture exposure treatment in 2/6 parts of the body once a week for 1 month. Treatment duration of exposure to laserpuncture for 2 seconds, 4 seconds, 6 seconds, 8 seconds and control. The results showed that exposure to different laserpuncture beams gave highly significant different results on the fertility of tawes fish. The best treatment was exposure to laserpuncture for 6 seconds with a fertilization value of 95.20%. The water quality during the study was within normal limits for tawes life which included a temperature of 27.80C, dissolved oxygen (DO) of 7.58 mg/l and a pH of 7.65.

The effect of soft laser on erythrocytes of patients with breast cancer

The effect of soft laser on erythrocytes of patients with breast cancer

Soft laser and its application areas in oral surgery and stomatology

Low-level lasers (LLL) are used in the stomatology and oral surgery field of dentistry. Due to the biostimulation or biomodulation and photodynamic effect, the healing process of soft-tissue and bone wounds can be accelerated. The indication in oral surgery is to achieve a faster bone healing after tooth extraction, but also to improve the osseointegration in implants. The advantage in stomatology is pain alleviation and improved healing in aphthae and cold sores as well as symptom relief in mucositis. Due to the use free of side effects and low time effort, the LLL offers high patients' comfort.

The Effects of Soft Laser on Some Blood Parameters of Patients with Breast Cancer

The Effects of Soft Laser on Some Blood Parameters of Patients with Breast Cancer

Surgical excision of lobular capillary hemangioma with laser under N2O/O2 conscious inhalational sedation in an uncooperative pediatric patient, Frankl class 2: a case report

&lt;p&gt;Here, we report a case of lobular capillary hemangioma (LCH) associated deleterious oral habit on lateral border of the tongue in an anxious child excised under nitrous oxide (N&lt;sub&gt;2&lt;/sub&gt;O) sedation using soft tissue diode laser. LCH are rapidly growing vascular lesions and are common occurrence over tongue, but to be associated with deleterious oral habit is an uncommon occurrence. A 9-year-old female child was brought to the dept by her parent due growth on the tongue. The child was quite anxious and displayed negativity towards examination and treatment. The child was classified as Frankl 2 on the behaviour rating scale. A pre-anaesthetic check-up was carried out on the child as it was envisaged to carry out the treatment under inhalational sedation. The anaesthesiologist along with crash cart were present throughout the procedure to cater to any adverse events. The excision was done with 980-nm soft tissue diode LASER to reduce intraoperative haemorrhage, enhance patient comfort and improve postoperative healing. A novel combination of inhalational sedation with soft tissue diode LASER with all necessary precautionary protocols as used in management of this child may be an useful approach for successful and uneventful management. To highlight the importance of proper use of pharmacologic methods in behaviour management as well as surgical techniques for better management of these vascular lesions in children.&lt;/p&gt;&lt;p&gt;&lt;strong&gt; &lt;/strong&gt;&lt;/p&gt;

Comparative Evaluation of Azadirachta indica (Neem) Chip and Soft Tissue Diode Lasers as a Supplement to Phase I Periodontal Therapy in Localized Chronic Moderate Periodontitis: A Randomized Controlled Clinical Trial.

Introduction The current trial aimed to assess and compare the efficacy of neem chip and diode laser as a local drug delivery (LDD) agent as a supplement to phase I periodontal therapy in treatment of localized chronic moderate periodontitis. Materials and Methodology. Fourteen systemically healthy participants with 4–6 mm deep periodontal pockets at least in three quadrants (with no alveolar bony defect amenable to respective or regenerative osseous surgery, as seen in orthopantomograph) were selected for the trial. One week after phase I therapy, 10% absorbable chip of neem (commercially prepared by staff of a pharmacy college, Sheriguda, India) was placed in the periodontal pocket on one site, and soft tissue diode laser pocket sterilization was performed on the other site of the arch. Remaining one site was considered as a control. Parameters recorded clinically were plaque index (PI), papillary bleeding index (PBI), probing pocket depth (PPD), and relative attachment level (RAL) measured at baseline, 21st day, and one month postoperatively. Results Statistically significant improvements were observed in all clinical parameters at one month as compared to baseline for both treatment groups. Conclusion Neem chip supplemented with phase I therapy showed best improvement in clinical parameters followed by laser supplemented with phase I therapy in comparison to phase I therapy alone at one month follow-up. Clinical Significance. Neem chips are nature's products, affordable without side effects, with a potential to be used as a local drug delivery agent in treating moderate chronic periodontitis.

LOW LEVEL LASER THERAPY: AN INSIGHT TO THE PERIODONTAL APPLICATIONS

Low-level laser treatment, also called 'Soft Laser Therapy' has been used for more than three decades in the health system. It was rst introduced by Mester and his colleagues. In-vivo and in-vitro experiments have shown that low-level laser is capable of speeding up repair process and also reduce post-operative pain. The application of low-level laser along with gingivectomy would result in improved clinical conditions and faster repair. Low level laser application following coronally advanced ap may enhance treatment prognosis in cases of gingival recession. Low-level laser would also be useful in pain reduction and wound healing in case of gingival grafting procedures. The differences in research results may be attributed, bearing in mind that low-level laser radiation depends on various parameters such as wavelength used, power, energy density, radiation duration, radiation model and the distance from the site under radiation. Hence, this review focuses on the mechanism of low level lasers, their effects on various tissues and the applications in the eld of periodontology.

Mass Spectrometry (MS/MS) and Proteomics: A High-Performance Bioanalytical Technique for the Peptide Drug Discovery

Drug discovery research focuses on the Rational Drug Design (RDD) concepts, and the major obstacles in the drug discovery process are the lack of target specificity and selectivity. The realization of higher target selectivity of peptide drugs has promoted peptide research. Rapid growth in genomics along with recombinant DNA (rDNA) technology and gene expression studies stimulated the peptide research. The promising use of peptide therapeutics demands sensitive and selective quantification methods. Protein sequencing and proteomic investigations can be successfully accomplished through the Mass Spectroscopy (MS) based methods. Mass spectroscopy-based soft ionization methods, namely, electrospray ionization (ESI) and Matrix-Assisted Laser Desorption/Ionization (MALDI), offer high-throughput sequencing to provide the characterization (sequence and structure) of intact proteins/peptides. The advent of tandem Mass Spectrometry (MS/MS) along with data acquisition methods are the basis for the evolution of peptide therapeutics research. The evolution of data science helped in developing computational proteomics, which assists in the quantitative determination of protein samples. This review narrates the role of mass spectrometry in the peptide drug discovery particularly in the sequence characterization along with the latest developments, such as computational proteomics.

An Efficacy of Photobiomodulation of 850 nm on Pain Reduction in a Human Oral Capsaicin Pain Model.

Objective: The aim of this clinical trial was to evaluate the efficacy of photobiomodulation (PBM) to reduce oral pain initiated by capsaicin. Background: PBM has been used for pain reduction in oral regions; however, its clinical efficacy to alleviate burning sensations is still unknown. Methods: This clinical study was divided into two phases. There were 10 subjects in each phase. In Phase I, the burning sensation was stimulated using 0.05% in 95% ethanol capsaicin (prepared by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand) on filter paper and recorded with a 10-cm horizontal visual analog scale (VAS) every 1 min until the subject experienced no more pain. The area under the curve (AUC) of the graph between VAS and time was calculated. The washout period was 24 h. In Phase II, a crossover clinical trial was conducted in subjects who received the pain model as stated. Four interventions were included: (1) PBM before & after pain stimulation, (2) PBM before pain stimulation, (3) PBM after pain stimulation, and (4) Placebo. For PBM intervention, a single exposure of 850 nm Laser (AsGaAl; TOP 250 Soft Laser, Berkmed Medikal, distributed by Medical Innovation-MI, Istanbul, Turkey) with 0.10 cm2 spot size laser probe continuous wave at 1 W/cm2 and 30 J/cm2 for 30 sec was used. Results: In Phase I, there was no statistically significant difference (p = 0.09) between the average AUC of day 1 [55.63 (23.38)] and day 2 [48.99 (27.20)]. In phase II, there was no statistical difference (p = 0.20) of the average AUC among the interventions and the placebo; group 1 [56.24 (30.11)], group 2 [45.46 (45.46)], group 3 [64.15 (32.89)], and group 4 [58.59 (30.16)]. Conclusions: The human capsaicin model was suitable for a crossover design clinical trial. The PBM used in this study did not reduce oral burning sensations from capsaicin.

Abstracts from the World Federation for Laser Dentistry—Asia Pacific Division Virtual Conference July 16–17, 2021

Abstracts from the World Federation for Laser Dentistry—Asia Pacific Division Virtual Conference July 16–17, 2021

Comparison of Success Rate of Estrus and Pregnancy between Laser Puncture and Intra Vaginal Progesterone Sponge in Bali Cattle

The purpose of this study was to find a method of determining laser models and intra vaginal progesteronesponge in Bali Cattle. An assessment was carried out at Sapi Loka Grati, Pasuruan, Indonesia. The parametersobserved included the symptoms of estrus, response of estrus, and the level of pregnancy. Twenty Balinesecows were divided at random in two groups. P1 treated with laser puncture at a certain point and P2 treatedwith 1.5 g of progesterone + 10 mg of estradiol benzoate intra vaginal sponge. The diagnosis of subsequentpregnancies was done by ultrasonographic examination on day 40th after artificial insemination (AI). Thelaser used in this study was a soft laser with power supply specifications as follows: 50 Hz, 220 Volts, 50Watts, ranging from 0.2 Joules to 0.5 Joules. These lasers include semiconductor lasers and laser probes.Three acupuncture points were used (GV 4, BL-22, and GV-2). Furthermore, the intra vaginal progesteronesponge used was made from silicone plastic similar to the drugs containing medroxyprogesterone acetate.Data testing was done by using the T-test. Results demonstrated that the P1 responses of estrus appeared onday 3rd, while P2 on day 9th. There was no significant difference (p >0.05) in the accumulation of estrus andpregnancy. In conclusion, estrus synchronization and pregnancy rate could be efficiently achieved in Balicattle using either laser puncture or intra vaginal progesterone sponge. However, laser puncture treatmentinduced more spread estrus with higher pregnancy rate.

Lasers in Orthodontics – A Review

Introduction: In past few years, there is huge improvement in orthodontics. Different innovation in orthodontics made the procedure easier and less time consuming. Application of laser is one of them. Many types of dental lasers are currently available that can be efficiently used for soft and hard tissue applications in the field of orthodontics. Two types of lasers are there. One is hard tissue laser, and another is soft tissue laser. Laser therapy is advantageous because it often avoids bleeding, can be pain free, is non‑invasive and is relatively quick. The high cost is its primary disadvantage. The purpose of this article is to provide an overview regarding safe and proper use of soft-tissue lasers in orthodontics

Low Level Laser Therapy: Silent Revolution in Dentistry

In virtually every area of dentistry the advent of lasers and more precisely soft lasers was revolutionary. Advantages of this particular laser is multidirectional, and thus the results are multifold. A major purpose in current dentistry is the non-invasive therapy in oral diseases. The search for a new treatment promotes a thorough review of the laser technology that currently provides opportunities for their use in all fields of dental practice. Improved knowledge and ongoing work in this direction have culminated in a stronger awareness of of Low Level Laser Therapy’s (LLLT’s) stimulatory, anti-inflammatory and biogenerative impacts, not just on soft tissue but also on hard tissue. Laser results have not yet been extensively studied, but they have the potential to become recognized as a better alternative than conventional therapy. The aim of this article is to put the use of LLLT in the field of dentistry into perspective through exploring the concepts of low-level laser therapy and their effect on soft and hard tissues in the oral cavity at a subclinical stage. This in turn reduces the dependence on medications and with it reduces the occurrences of drug interactions or complications.

EFFECTIVENESS OF DIODE LASER IN PERIODONTAL THERAPY: A CLINICAL STUDY

Now-a-days, almost every specialty area of dentistry is utilizing the powers of laser energy for therapeutic purposes. The diode laser is the most popular soft tissue laser and commonly used in dentistry. A diode laser with wavelengths ranging from 810 to 980 nm in a continuous or pulsed mode is used as a possible instrument for soft tissue surgery in the oral cavity. The 810 nm devices predominate in dentistry, whereas 810 nm is specific for hemoglobin absorption. Diode lasers have many advantages over traditional surgical and therapeutic techniques in addition to providing bloodless surgical and postsurgical course with minimal swelling, scarring, and others things noted are less pain, excellent hemostasis, rapid wound healing and bactericidal action with the minimally invasive procedure. In the present paper, we discuss various uses of the diode laser such as frenectomy, implant surgery, vestibular deepening, and gingival enlargement procedures in dentistry.&#x0D; Keywords: Diode laser; frenectomy, Implant Surgery, Vestibular Deepning, Gingival enlargement

The effects of laserpuncture on gonadal maturity and sperm quality of male striped catfish (Pangasianodon hypophthalmus)

Laserpuncture is one of the applicative technologies used mainly in animal and fish reproductions. Laserpuncture technology has been used to improve gonadal maturity and sperm quality in fish rapidly. This study aimed to determine the effects of different laserpuncture doses on gonadal maturity and sperm quality of male striped catfish. Males striped catfish (800–900 g/fish body weight) and I gonadal maturity stage were used. Semi-conductor soft laser was used with doses of 0.2-, 0.4-, and 0.5-J, while the negative control (without the laserpuncture and the ovaprim™) and only the ovaprim™ were used as a comparison treatment, respectively. The soft-laser was treated on reproductive acupoint every week for four weeks, while the ovaprim™ was administered by intramuscular injection at dose of 0.2 mL/kg fish in final rearing period. Fish was reared in hapa at the controlled pond. Fish was fed with a commercial feed containing 32% crude protein. Gonadal maturity, gonadosomatic index (GSI), hepatosomatic index (HSI), and sperm quality of male striped catfish were measured in the final rearing period. The results showed that the laserpuncture on the reproductive acupoint had a highly significant effect (P < 0.01) on the gonadal maturity, GSI, HSI, and sperm quality of male striped catfish. In terms of the gonadal maturity, laserpuncture doses treatment of 0.4 and 0.5-J gave the most mature IV stage. While the highest levels of GSI and HSI were found in 0.5-J of laserpuncture dose, which was 2.17% and 1.54%, respectively. In addition, the best sperm qualities were observed in 0.5-J of laserpuncture dose, which were 81.75% motility, 82.75% viability, and 7.0 × 109 cell/mL concentration. These results suggest that the laserpuncture can accelerate a gonadal maturity and improve sperm quality in male striped catfish.

Treatment of Dentinal Hypersensitivity using desensitizing agents plus soft laser irradiation. A Randomized Comparative Clinical Trial

This study aimed to evaluate the efficacy of diode laser combined with two in-office desensitizing agents 8% Arginine-CaCO3 and 1.23 NaF varnish on reducing dentine hypersensitivity (DH) in periodontitis patients. Materials and Methods: Forty patients having stage I and Stage II periodontitis were selected according to the criteria of AAP (2017) with complaint of (DH) after routine periodontal therapy . Patients were randomly divided into two groups: group1- received NaF varnish then diode laser application at 1 W (PW) ,(CW) for 30 seconds using 320µ fiber. Group-2, treated with 8%Arginine-CaCO3 plus same laser irradiation. Each tooth received three application Dentine hypersensitivity evaluation was by tactile, air-blast, and thermal stimuli and measured using VAS scores. The patient’s response was recorded at baseline, one month and 3 month after the application.Results and conclusions: The results were statistically analyzed, and it was found that 8% Arginine-CaCO3 plus laser treatment was more effective than 1.23% NaF-varnish plus laser at time intervals. Sensitivity score differences between the groups were statistically significant at one and three months. The 8% Arginine-CaCO3 group exhibited statistically significant reduction in dentine hypersensitivity on three stimuli at baseline to one and three months. It was concluded that 8% Arginine- CaCO3 plus laser irradiation is more effective than 1.23% NaF varnish plus laser irradiation in reduction of patients’ pain in periodontitis patients.

Experimental alternative to investigate the radiation-induced radical chemistry at the molecular level

SynopsisThis project aims to develop an original approach to investigate the damage at the molecular level on biomolecules from the indirect irradiation effects. We will construct a new device to put biomolecules in the gas phase, based on the soft laser desorption from microdroplets directly into vacuum, and this source will be coupled with an irradiation platform.

Atmospheric Pressure Ultraviolet Laser Desorption and Ionization from Liquid Samples for Native Mass Spectrometry.

Understanding protein structure is vital for evaluating protein interactions with drugs, proteins, and other ligands. Native mass spectrometry (MS) is proving to be invaluable for this purpose, enabling analysis of “native-like” samples that mimic physiological conditions. Native MS is usually performed by electrospray ionization (ESI) with its soft ionization processes and the generation of multiply charged ions proving favorable for conformation retention and high mass analysis, respectively. There is scope to expand the currently available toolset, specifically to other soft ionization techniques such as soft laser desorption, for applications in areas like high-throughput screening and MS imaging. In this Letter, observations made from native MS experiments using an ultraviolet (UV) laser-based ion source operating at atmospheric pressure are described. The ion source is capable of producing predominately multiply charged ions similar to ESI. Proteins and protein complexes were analyzed from a native-like sample droplet to investigate the technique. Ion mobility-mass spectrometry (IM-MS) measurements showed that folded protein conformations were detected for ions with low charge states. This observation indicates the source is suitable for native MS analysis and should be further developed for higher mass analysis in the future.