Soft Drink Samples Research Articles

Potential human health risk assessment of potentially toxic elements intake via consumption of soft drinks purchased from different Egyptian markets

ABSTRACT Consumption of soft drinks with high potentially toxic elements concentrations may lead to acute or chronic poisoning. Soft drinks have been shown to contain traces of some potentially toxic elements such as cadmium, lead, mercury arsenic, zinc, etc. which may be due to environmental pollution from the surface and underground water, food and fruits utilised during production, and can be toxic for humans. In this study, Quadrupole Inductively Coupled Mass Spectrometer (Q-ICP-MS) was used in a simple validated method of analysis for direct determination of Al, Sb, As, Cd, Cr, Co, Cu, Fe, Pb, Mn, Hg, Ni, Sn, and Zn in soft drink samples. Analysis of 130 samples covering 14 different brands of popular soft drinks in Egypt to assess whether the intakes complied with the upper permissible levels for tested metals was performed. The results showed that all analysed samples were free from any detectable amount of Hg, while the mean concentrations, in µg/L, were as follows: 1.48 (Cr), 1.96 (As), 0.77 (Sb), 0.53 (Co), 20.27 (Mn), 108.83 (Fe), 0.52 (Cd), 13.18 (Cu), 8.55 (Ni), 20.00 (Sn), 80.09 (Al), 36.54 (Zn), and 1.05 (Pb). The health risk assessment was evaluated for estimated weekly intake, the hazard quotient, the hazard index and the target cancer risk using the Food and Nutrition Board, FAO/WHO and USEPA recommendations. The weekly intakes of all elements were found to be significantly below the recommended tolerable levels. Also, the hazard quotient, the hazard index and the target cancer values for all elements were found to be lower than acceptable guideline values, which indicated that there was no any non-carcinogenic or carcinogenic effects may occur.

Designing and constructing a micro flow injection system (μFIA) for phosphate determination in soft drinks

Lab. made micro flow Injection system (μFIA) was designed which supplied with two microcontroller UNO and Mega types. These two microcontroller (Arduino) were provided with home- made software programs.The first one, type UNO used to control home- made mini peristaltic pump to drive reagents to detector. The other one was Mega type which was used as a data-logger to manipulate and recording the results as peak height corresponding the concentration by using Microsoft Excel 2010 program. The diameter of the tubes was 0.2mm i.d which used in all parts of manifolds The proposed μFIA manifold was used for phosphate determination in soft drinks samples which supplied from local market. The best conditions for the determination of phosphate by Murphy method was studied. linearity, detection limit, r.s.d% were 5-30 μg/ml,0.09 μg/ml and 0.48% respectively. The phosphate concentrations in the different soft drink samples were in the rang 250-825 μg/ml by using the standard addition method in order to eliminate all expected interferences. The home -made Semi- automated micro-flow injection analysis (μFIA) system was applied successfully for phosphate estimation with simple, high sensitive and accurate.

Tetrad vs. triangle test: A case study with Brazilian guarana soft drink

Discrimination sensory tests aim to identify if a difference between two similar stimuli is detected. In this study we compared the efficacy of Tetrads and Triangle tests in the difference detection between two samples of guarana soft drink, by means of the calculation of proportion of discriminators and thurstonian distance. Evaluated samples were produced by different syrup clarification methods (activated carbon and ionic exchange column). For each test 99 testers were used; Triangle test evaluated three samples, while Tetrad four samples, in complete randomized blocks. Only Tetrad test was able to detect significant difference between the samples (p<0.05), with a low proportion of discriminators and thurstonian distance inferior to perception limit, demonstrating that Tetrad test is more powerful and sensible than Triangle test.

PCN-222 metal\u2013organic framework: a selective and highly efficient sorbent for the extraction of aspartame from gum, juice, and diet soft drink before its spectrophotometric determination

In this paper, we describe synthesis and application of an iron porphyrinc metal–organic framework PCN-222(Fe) for solid phase extraction of aspartame, an artificial non-saccharine sweetener, from gum, juice and diet soft drink samples prior to its determination by spectrophotometry. The mesoporous MOF was synthesized solvo-thermally and characterized by Fourier transform-infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy, and Brunauer–Emmett–Teller techniques. To obtain the best extraction efficiency of aspartame, significant affecting parameters such as pH of sample solution, amount of the sorbent, type and volume of eluting solvent, and adsorption and desorption times were investigated and optimized. Under optimum conditions, the calibration graph for aspartame was linear in the range of 0.1 to 100.0 mg.L−1 and relative standard deviation of aspartame was 1.7% (n = 7). Limit of detection of method calculated as 0.019 mg.L−1 and the enrichment factor of 350 folds was obtained. Adsorption capacity of synthesized sorbent was found to be 356 mg.g−1. Hierarchical porosity, the eight terminal–OH groups of the Zr6 node, and hydrogen bonding possibly play vital role for selective adsorption of aspartame. The optimized method was successfully applied to the determination of aspartame in real samples with reasonable recoveries (> 98%).

Quality assessment of some selected locally produced and marketed soft drinks in Ghana

Soft drinks have been reported to contain some toxic heavy metals beyond the acceptable limits in some countries. Therefore, the objective of this study was to assess the quality characteristics of selected locally produced and marketed soft drinks. Three (3) different batches of 23 soft drinks were sampled from the Takoradi markets in Western Region of Ghana. Qualitative tests for the presence of reducing sugars, phosphates, alcohol and carbon dioxide in the samples were carried out. The heavy metal contents and physicochemical properties were also determined with Association of Official Analytical Chemists (AOAC) methods. The results indicated the presence of reducing sugars, carbon dioxide and the absence of alcohol in all the selected soft drink samples. The pH, total sugars, moisture, total soluble solids (TSS) and titratable acidity ranged from 2.42 – 3.44, 3.30 – 10.44%, 85.63 – 94.85%, 5.00 – 13.33°Brix, and 0.21 – 1.99% respectively. The concentration of heavy metals were also below detection limits in all samples. The quality of the selected soft drinks were within specifications prescribed by regulatory bodies.

Pemanfaatan Isolat Selulosa Ampas Tebu sebagai Chelating Agent (CAT) Zat Pewarna pada Jajanan Anak Sekolahan (JAS)

This study aims to detect synthetic dyes in JAS (School Children Snacks) using a chelating 
 agent (CAT) from bagasse waste. Instrumentation used in the analysis was spectronic 20D. 
 Sugarcane pulp cellulose products as CAT adsorbent rhodamine B coloring agent, metanil 
 yellow and tartrazine in JAS were applied to 10 types of soft drink samples circulating in the 
 market in Gorontalo City. The method used is the adsorption method. The adsorption capacity 
 produced by cellulose with 75% content and α-cellulose content was 37.8% for sample 1 (56%) 
 sample 2 (12%), sample 3 (83%), sample 4 (54%), sample 5 (45%) , sample 6 (54%), sample 7 
 (51%), sample 8 (27%), sample 9 (69%) and sample 10 (25%). The adsorption capacity 
 produced by cellulose was 88.4% and α-cellulose content was 45.2% for sample 1 (56%) 
 sample 2 (12%), sample 3 (83%), sample 4 (54%), sample 5 (45%) , sample 6 (54%), sample 7 
 (51%), sample 8 (27%), sample 9 (69%) and sample 10 (25%).

Levels of fluoride in bottled soft drinks marketed in Addis Ababa, Ethiopia

The main objective of this study was to determine the levels of fluoride in soft drinks (Coca Cola, Pepsi, Mirinda, Fanta and Sprite) marketed and widely consumed in Addis Ababa, Ethiopia. Three glass-bottled and three plastic-bottled soft drink samples from each brand were purchased randomly from Arat Kilo, Shiromeda and Shola supermarkets, kiosk and tea houses in Addis Ababa, Ethiopia. Levels of fluoride in the soft drink samples were determined by fluoride ion selective electrode. The method was validated by spiking test which provided percentage recoveries of fluoride in the soft drinks in the range 91-96%. The mean fluoride concentration (mg/L) in the glass-bottled and plastic-bottled soft drink samples, respectively, were: Coca Cola (0.03±0.01, 0.06±0.01), Pepsi (0.23±0.01, 0.10±0.01), Mirinda (0.21±0.02, 0.09±0.01), Fanta (0.03±0.01, 0.05±0.01) and Sprite (0.04±0.01, 0.27±0.01). Pearson correlation showed that the levels of fluoride in the soft drinks were found to correlate positively with each other, which indicates similar source of main component (the water used for dilution). The low levels of fluoride in the soft drinks may not impose health risk in the adults but excessive consumption of soft drinks regularly by the children may result in dental fluorosis. KEY WORDS : Fluoride, Coca cola, Pepsi, Mirinda, Fanta, Sprite Bull. Chem. Soc. Ethiop. 2019 , 33(2), 203-213. DOI: https://dx.doi.org/10.4314/bcse.v33i2.2

Method validation for direct determination of some trace and toxic elements in soft drinks by inductively coupled plasma mass spectrometry

ABSTRACT The consumption of soft drink beverages has increased in the last few years around the world and it is related to the diversity of brands and flavours available, increasing also the risk of ingestion of compounds considered non-beneficial to the health of consumers. In this study, fast, easy and simple method of analysis for direct determination of As, Pb, Cd, Sb, Hg, Cu, Zn, Fe, Al, Cr, Sn, Co, Mn and Ni in soft drink samples using quadrupole inductively coupled plasma mass spectrometry (Q-ICP–MS) was validated. The estimated detection limits, practical quantification limits, linearity (linear dynamic ranges and method linearity), accuracy (trueness and precision) and measurement uncertainty parameters were studied under optimised (Q-ICP–MS) conditions. The method showed that the estimated detection limits were varied between 0.02 and 2.403 µg/L, and the quantification limits were varied between 0.5 and 20 µg/L. The mean recoveries ± standard deviations at different spiking levels were varied between 75.03 ± 0.62% and 117.07 ± 2.83% and the coefficients of variation were varied between 0.49% and 9.79%. The method trueness was confirmed by using four different certified reference materials (soft drinks and treated water) purchased from FAPAS (Food Analysis Performance Assessment Scheme) and all obtained results were within satisfactory ranges and had acceptable recovery and Z-score values. The method precision, in terms of relative standard deviation, was below 4.88%. The method uncertainty expressed as expanded uncertainty of all validated elements was found to be ≤22.52%. The results obtained make the method suitable for accurate determination of validated elements in different kinds of soft drink samples at these low concentration values. Validated method was used for the determination of metallic contaminants in 40 commercial soft drink samples and the results were compared with the provisional guideline of the elements stated by Egyptian, WHO and European standards in drinking and potable bottled natural mineral water.

Developed of a Green Water Switchable Liquid–Liquid Microextraction Method for Assessment of Selenium in Food and Soft Drink Samples by Using Hydride Generation Atomic Absorption Spectrometry

In the current study, we have developed a green water switchable liquid–liquid microextraction method for separation, preconcentration, and estimation of selenium concentration in the real samples. First time introducing the water switchable liquid–liquid microextraction method to determine the trace level selenium in different food and soft drink samples. Water switchable medium was formed by the reaction of diethylenetriamine base when exposed to uniformed pressure of carbon dioxide. After being exposed to carbon dioxide, water switchable medium reversibly exchanges in two separated aqueous and organic phases. Advantages of carbon dioxide uses are cheap, environmental friendly, non-accumulation, removable, and require the opaque materials for operating container. Water switchable phenomena occurred easily from low polarity to high polarity organic solvent. Experimental variables of the water switchable liquid–liquid microextraction (LLME) method were optimized into its optimum values such as pressure, pH, centrifugation speed, extraction time, and concentration of complexing agent. The certified reference material of Canada Lake Water (TMDA-53.3) and CS-M-3 Mushroom (Boletus edulis) was used for validation of the present water switchable LLME method. Enhancement factor and limit of detection were obtained 85.5 and 0.018 μg kg−1, respectively. Developed green water switchable LLME method was successfully applied for assessment of total selenium in tomato, pumpkin seed, mushroom, garlic, rice, pistachio, chickpea, hazelnut, walnut, apple juice, and ice tea samples.

A novel thread-based microfluidic device for capillary electrophoresis with capacitively coupled contactless conductivity detection

This work describes a novel thread-based microfluidic device to perform capillary electrophoresis separation and capacitively coupled contactless conductivity detection. Polyester threads with a diameter of about 315 μm were assembled (stretched) in a 3D-printed platform containing solution reservoirs and platinum electrodes. Separation of potassium, sodium, and lithium ions was achieved within 1 min with good peak resolutions and limits of detection close to 1 μmol L−1. Inversion of the electroosmotic flow direction by adding a cationic surfactant in the running electrolyte was demonstrated. The applicability of the thread-based microfluidic devices was successfully evaluated by the determination of sodium and potassium ions in commercial samples of diet soft drinks.

Evaluation of Mineral Element and Sugar Contents of Soft Drinks in Nigeria

This research work analyzed and evaluated the sugar and mineral elements contents of soft drinks consumed daily in Nigeria using standard methods. The sugar content and mineral element were determined. The sugar content varies from 22.8.4mg/L to 274.4mg/l and means sugar level varies from 30.4mg/l to 271.07mg/l. The mineral elements analyzed were Na, K, Ca, Mg, Ni, Fe, Cu, Zn, Cr, Pb and Cd. From the results it can be seen that the macro element (Na and Ca) which are essential mineral reduces rapidly in all the samples of soft drink after 4 weeks. Results of one way ANOVA above indicates that there was a significant difference in the sugar test between sample 1 to sample 7 at p<0.05. (F= 1104.637, p= 0.000). Keywords : Soft drinks, mineral elements, sugar content, ANOVA

Construction of novel electrochemical sensors based on bimetallic nanoparticle functionalized graphene for determination of sunset yellow in soft drink

Three novel electrochemical sensors constructed by Pd-Pt bimetallic nanocages, Pt-Cu bimetallic nanoframes and Co-Ni bimetallic nanoflowers and poly (diallyldimethylammonium chloride) (PDDA)-dispersed graphene (Gr) were applied to determine sunset yellow (SY, E110). The PDDA-Gr-(Pd-Pt), PDDA-Gr-(Pt-Cu) and PDDA-Gr-(Co-Ni) nanocomposites formed different nanostructure with unique features and were characterized by transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDX). The special morphology of the three bimetallic nanoparticles endows the designed electrochemical sensors with large active areas and good electrical conductivity, which provides more active sites for the target analyte and improves the sensitivity of the determination of sunset yellow. The developed sensors were investigated and compared with each other on the determination of SY by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under optimal experimental condition, the anodic peak currents of sunset yellow on three modified electrodes was linearly correlated with the concentration of sunset yellow in the ranges of 0.02–10.0, 0.02–10.0, 0.008–10.0μM, respectively. The limits of detection (LOD) of SY were 6.0, 4.0 and 2.0nM, respectively. In addition, the three new proposed sensors in this study were successfully applied to determine SY in real soft drink sample. This study provides a new platform for designing sensitive electrochemical sensor based on non-noble metals with low cost.

Surface enhancement of porous alumina microfibers toward electrochemical sensing of chlorogenic acid

Porous alumina microfibers with a diameter of 125 nm were prepared through hydrothermal reaction in urea solution using aluminium nitrate as the precursor, and characterized using transmission electron microscopy and scanning electron microscopy. The mechanism of oxidation of chlorogenic acid was examined using cyclic voltammetry, indicating a reversible two-electron process. Compared with the bare carbon paste electrode (CPE), the oxidation signals of chlorogenic acid were greatly enhanced with the alumina microfiber-modified CPE. Chronocoulometry experiments indicated that the accumulation of chlorogenic acid on the surface of the alumina microfibers was obviously improved relative to that on the bare electrode, which is attributed to the numerous micropores and regular fiber-like structures. Based on the signal amplification ability of the porous alumina microfibers, a novel electrochemical sensing platform was developed for chlorogenic acid. The linear range was 10–2000 μg L−1, and the limit of detection was evaluated to be 5.0 μg L−1. The practical application of this new method was demonstrated using honeysuckle and soft drink samples. The results were consistent with the values obtained by high-performance liquid chromatography.

An automated homogeneous liquid-liquid microextraction based on deep eutectic solvent for the HPLC-UV determination of caffeine in beverages

This work presents an automated homogeneous liquid-liquid microextraction procedure for separation of caffeine from beverages. The microextraction procedure involved mixing sample and water-miscible deep eutectic solvent (DES, choline chloride with phenol at a 1:3 molar ratio) into a mixing chamber of a flow system resulting formation of homogeneous solution. Injection of aprotic solvent (tetrahydrofuran) into homogeneous sample solution promoted the fine DES droplets formation and caffeine extraction into DES phase separated. The automated homogeneous liquid-liquid microextraction procedure was coupled with a HPLC-UV system. The conditions of caffeine microextraction and its HPLC-UV determination in DES phase were studied and optimized. Under optimal experimental conditions the linear detection range and limit of detection, calculated from a blank test, based on 3σ were found to be 0.1–200 mg L−1 and 0.03 mg L−1, respectively. The application of the developed procedure was demonstrated in the determination of caffeine in commercial soft drinks samples.

Spectrophotometric Analysis of Preservatives and Coloring Agents in Orange Soft Drink and Orange Juice Samples

A rapid and simple UV-Vis spectrophotometric method was used for the determination of the level of sodium benzoate and yellow 6 in 14 different batches of orange soft drink samples and 7 different batches of orange juice samples commercially available in local markets of Bangladesh. The standard samples of sodium benzoate and yellow 6 in water were used to make calibration curves and the correlation coefficient (r2) of the calibration curves were found to be 0.9993 and 0.9953 for sodium benzoate and yellow 6, respectively. The wavelength of absorption maxima was 225 nm for Sodium benzoate and 429 nm for yellow 6. All 14 analyzed soft drinks samples contained yellow 6 and sodium benzoate in the range of 2.90-162.51 mg/L and 94.06-387.66 mg/L, respectively. The highest amount of sodium benzoate and yellow 6 were found in the sample AMR (387.66 mg/L) and BRC (162.51 mg/L), respectively. Among the 7 orange juice samples yellow 6 were found in the range of 28.03-102.66 mg/L and the highest amount was in the sample OJ1 (102.66 mg/L). The reproducibility and repeatability of the method was very satisfactory with low value of RSD in a range of 0.05 to 4.17% and 0.05 to 4.07%, respectively.&#x0D; Dhaka Univ. J. Sci. 66(2): 135-137, 2018 (July)

Fabrication of magnetite-functionalized-graphene oxide and hexadecyltrimethyl ammonium bromide nanocomposite for efficient nanosorption of sunset yellow

A novel magnetic nanocomposite based on magnetite nanoparticles-functionalized-graphene oxide and hexadecyltrimethyl ammonium bromide has been synthesized (MAGO-CTAB) by a facile route for efficient, fast and sensitive binding with Sunset Yellow (SY). The MAGO-CTAB (27 ± 3 nm) has been successfully characterized by transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and vibrating sample magnetization techniques. The influences of different experimental parameters on the % SY removal efficiency were fully investigated. The adsorption rates of SY by MAGO-CTAB were conducted by fitting the experimental data to four kinetic models. Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D–R) adsorption isotherms were applied to study SY removal. The adsorption-desorption stability performance of the novel magnetic nanosorbent was evaluated and confirmed after 5 cycles. The designed MAGO-CTAB was successfully utilized for removal of SY from different food and soft drink samples with excellent recoveries values (98–103%).

Application of molecularly imprinted polymer in solid-phase microextraction coupled with HPLC-UV for analysis of dibutyl phthalate in bottled water and soft drink samples

In this study the optimized molecularly imprinted polymer as a highly selective sorbent toward dibutyl phthalate was used in molecularly imprinted solid‐phase microextraction (MISPME) method as a clean-up and pre-concentration procedure before analysis by high performance liquid chromatography equipped with UV detector (HPLC-UV). The calibration of method was done in canned mineral water and soft drink as free dibutyl phthalate matrices. The calibration curves were plotted in the range of 0.01-2 µg mL−1with linear equations of y = 727.41x + 2.1706 (R2 = 0.9999) and y = 805.96x – 2.9927 (R2 = 0.9999) in canned mineral water and soft drink, respectively. Also limit of detection (LOD) and the limit of quantification (LOQ) value were 3 ng/mL and 0.01 µg/mL, respectively. The recoveries for extraction of dibutyl phthalate were in the range of 90.29% – 98.06%. Finally, calibrated MISPME method coupled to HPLC-UV (MISPME-HPLC-UV) was successfully developed for dibutyl phthalate residue control in commercial plastic bottled mineral water and soft drink samples sold in the market of Mashhad, Iran. Unfortunately, all commercial beverage samples had DPB residueabove the limit of quantification. The results indicated that the calibrated MISPME-HPLC-UV method can be successfully applied for DBP analysis in bottled beverage samples.

Multi-walled carbon nanotubes modified with iron oxide and silver nanoparticles (MWCNT-Fe3O4/Ag) as a novel adsorbent for determining PAEs in carbonated soft drinks using magnetic SPE-GC/MS method

The synthesis of compounds with an excellent adsorption capability plays an essential role to remove contaminants such as phthalic acid esters (PAEs) with potential carcinogenic characteristics from different food products. In this context, for the first time, a novel adsorbent (MWCNT-Fe3O4/Ag) was synthesized by using iron (magnetic agent), and silver (catalytic and surface enhancer agent) to further approach in a magnetic SPE-GC/MS method for determining of PAEs in carbonated soft drink samples. The limit of detection (LOD) and limit of quantification (LOQ) values of MSPE-GC/MS were determined in six PAEs as a range of 10.8–22.5 and 36–75 ng/L, respectively. Also, the calibration curves of PAEs were linear (R2 = 0.9981–0.9995) over the concentration level of 10.000 ng/L and the recoveries of the six PAEs were ranging from 96.60% to 109.22% with the RSDs less than 8%. Moreover, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD), vibrating sample magnetometry (VSM) and transmission electron microscopy analyses (TEM) were utilized to characterize the produced MWCNT-Fe3O4/Ag. Based on the findings, the surface of MWCNT is relatively uniform, which became coarser after loading with Fe3O4/Ag particles. Also, EDX spectrum showed the carbon (C), iron (Fe), oxygen (O), Ag and copper (Cu) are the main components of synthesized MWCNTs-Fe3O4/Ag. The successful adhesion of Fe3O4/Ag on the texture of MWCNTs using a co-precipitation method was confirmed by XRD and FT-IR assays. Additionally, excellent crystallinity and clear lattice nanocrystals fringes of prepared MWCNT-Fe3O4/Ag was demonstrated by TEM analysis. Based on the obtained VSM images, the prepared sorbent (MWCNT-Fe3O4/Ag) has the good magnetic performance for magnetic separation and extraction processes. It was concluded that the synthesized MWCNT-Fe3O4/Ag could be used as an efficient adsorbent for determining contaminants such as PAEs in different beverage samples.

The CoOOH-TMB oxidative system for use in colorimetric and test strip based determination of ascorbic acid.

The authors report that cobalt oxyhydroxide (CoOOH) nanoflakes possess intrinsic oxidizing ability to directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue colored product (oxTMB) even in the absence of H2O2 and oxygen. In the presence of ascorbic acid (AA), less of the blue product is formed because AA reduces oxTMB. These findings constitute a new scheme for colorimetric detection of AA. Absorbance, best measured at 652nm, linearly drops in the 10nM to 1μM AA concentration range, and the limit of detection is 5nM (at an S/N ratio of 3). The reaction is complete within <5s and highly selective. A strip test has been designed for fast and on-spot visual detection of AA. The method was applied to the direct estimation of AA in the microdialysate of brain, and also in soft drink samples. The strip test is considered to be a promising tool for the rapid screening of AA in brain and commercial samples. Graphic abstract Schematic of the CoOOH-TMB colorimetric system that exhibits a high selectivity for ascorbic acid (AA). A strip test has been designed for fast and on-spot visual detection of AA.

In vitro evaluation of soft drinks containing calcium lactate

Introduction: Over the past 2 years, the soft drink industry has seen a value growth of 11%. In total, 1.3 billion people in India drink nearly 5.9 billion liters of soft drinks per year making it one of the highest soft drink-consuming countries in the world. Hence, products such as calcium lactate which efficiently reduce the erosive action of drinks should be better incorporated in soft drinks to limit its adverse effects. Aim: The objective of this study was to assess the degree of dental erosion due to soft drinks with and without the presence of 5% calcium lactate. Materials and Methods: Three soft drinks (MaazaCoca-Cola, AppyFizParleAgro, and PepsiPepsiCo) and normal water were taken. A total of 40 dental blocks were randomly allocated into four groups. Surface loss (SL) was assessed using Digital Surfometry. Surfometry readings were taken before and after adding calcium lactate in the above soft drinks for definite time intervals. Data were analyzed using SPSS version 22. ANOVA was used to assess separately degree of erosion of tooth blocks in all the soft drinks samples with and without 5% calcium lactate. The paired t-test was used to determine the degree of SL within each group before and after adding 5% calcium lactate. Results: In the present study, PepsiPepsiCo had the highest acidic pH (2.7) and showed the highest degree of erosion with a mean value of 1.55 μ ± 0.07. After adding calcium lactate, the highest acidic pH was shown by AppyFizParle Agro (4.45); however, the degree of erosion was still the highest for Pepsi with a mean value of 1.36 μ ± 0.09. Later, each group was compared for SL among themselves with and without the presence of calcium lactate. AppyFizParleAgro showed the highest degree of erosion with a mean value of 0.22 μ ± 0.08. P ≤ 0.05 was considered as statistically significant. Conclusion: The erosive action of soft drinks varies widely. The results demonstrate that the addition of 5% calcium lactate to soft drinks significantly decreased dental erosion.