To establish and validate the determination method of the content of sodium ion and potassium ion in Dan Hong injection by atomic absorption spectrometry (AAS). [Methods] Microwave digestion system was applied to deteriorate samples, and the content of sodium ion and potassium ion was determined by AAS. [Results] The calibration curve of sodium was liner (r=0.9998) in the range from 0.1to0.6mg/L. The average recovery was 101.1% with RSD 1.8%. The limit of detection was 5.3μg/L. The calibration curve of potassium was liner (r=0.9998) in the range from 0.1 to 0.8mg/L. The average recovery was 99.8%with RSD 2.3%. The limit of detection was 7.7μg/L. The determination results of 10 batches Dan Hong injection sample showed that the content of sodium ion was 562.3 to 734.7 mg/L and potassium ion was 206.9 to 314.4mg/L. [Conclusion] The method is simple and accurate and can be used for determination of the contents of sodiumion and potassium ion in Dan Hong injection. The accurate quantitative determination method can be promoted to quality control of Dan Hong injection