Sodium Chloride Concentration Research Articles

Oral 10% concentrated sodium chloride for preoperative preparation of peroral endoscopic myotomy in achalasia of cardia: a simple and effective method

Oral 10% concentrated sodium chloride for preoperative preparation of peroral endoscopic myotomy in achalasia of cardia: a simple and effective method

Applications of integrated response surface methodology statistic techniques and artificial neural network‐based machine learning to optimize residual chlorine production and energy consumption

AbstractA multifactor interaction study was performed using the combined response surface methodology and an artificial neural network on the operational parameters and their influence on residual chlorine production. The operating variables, sodium chloride concentration, electrical potential, electrolysis time, and electrode gap, were evaluated over the response, residual chlorine and energy consumption. The results indicated that the optimum value for residual chlorine was 2450 mg/L achieved at an electrical potential of 8.8 V for 25 min in the presence of 25 g/L of sodium chloride and an electrode distance of 1 cm, and the optimum corresponding energy consumption was measured at 21.76 kWh/L. The study reveals that electric potential, sodium chloride concentration, and electrolysis time positively influence residual chlorine production. ANN models showed superior prediction ability compared with RSM models. This suggests electrolysis can be used for active chlorine production from saline solutions, potentially for industrial applications and water disinfection.

Experimental and theoretical investigation to optimize the performance of solar still

The cheapest and pollution-free method of producing drinkable water is solar distillation utilizing solar stills. However, the solar stills operate at lower-than-optimal efficiency, presenting significant room for improvement. Researchers have identified various techniques, modifications, and improvements to enhance efficiency in solar distillation. Notably, a solar pond is integrated with the solar still to boost performance, with the solar pond dramatically enhancing solar efficiency. In this research, four factors were identified as significantly influencing the performance of the solar stills: the concentration of sodium chloride (NaCl) in the solar pond, mini solar pond zones connected to the solar still, and the angles of internal and external reflecting mirrors. Three levels for each parameter were chosen for our study. The Taguchi method was utilized to identify the optimum level of these four parameters that maximizes performance. The best combination for maximizing efficiency was identified as connecting the solar still to the lower converting zone of the mini solar pond, using water containing 4 kg of NaCl, setting the internal reflecting mirror at 0⁰, and fitting the external reflecting mirror above the solar pond at 140⁰. This combination achieved a daily efficiency increase to 57.14%. Theoretical values were compared with experimental outcomes, showing that the solar still operated most efficiently between 12:00 and 14:00 h. The findings suggest not only immediate applications for improving solar still designs but also a pathway for future research into cost-effective desalination processes.

THE INFLUENCE OF POTASSIUM CHLORIDE AND MAGNESIUM CHLORIDE ON THE COLOR AND SENSORY PROPERTIES OF COOKED CHEESE

The aim of this paper is focused on reducing sodium chloride content by partial replacement with potassium chloride and magnesium chloride in cooked cheese samples. For the production of cheese, standardized cow's milk from a domestic market producer was used, and the cheese was produced by heating the milk to a temperature of 95°C and coagulation with acetic acid. The one salted only with NaCl was designated as the standard sample, and the other samples were salted with combinations of salts in which NaCl reduction was performed: sample A1 had a ratio of 15% KCl:85% NaCl, sample A2 30% KCl:70% NaCl, sample B1 15% MgCl2:85% NaCl and sample B2 30% MgCl2:70% NaCl. The cheese samples were stored at + 4°C and color parameters and sensory properties were analyzed on the 1st, 3rdand 5thdays of storage. Based on the performed analyses, it was concluded that it is completely acceptable to replace sodium chloride with potassium chloride in the ratio of 15% KCl:85% NaCl. It is acceptable to replace sodium chloride with potassium chloride inthe ratio of 30% KCl:70% NaCl, with the note that on the 5thday of storage there is a gradual deterioration of the sensory properties compared to the samples analyzed on the 1stday of storage. Replacement of sodium chloride with magnesium chloride in the ratios 15% MgCl2:85% NaCl and 30% MgCl2:70% NaCl is not acceptable.As such, it is not recommended in the production of cooked cheeses due to the appearance of a metallic and bitter taste that is present in cheese samples from the 1st to the 5th day of storage. KEYWORDS:cooked cheese, sodium chloride, potassium chloride, magnesium chloride

STUDY OF THE TECHNOLOGY FOR OBTAINING A MIXTURE OF SILICOFLUORIDE AND SODIUM FLUORIDE FROM A BY-PRODUCT OF HYDROFLUORIC ACID PRODUCTION

The article presents the results of research on the technology of processing and utilization of by-product of hydrofluoric acid production – a mixture of fluorosilicic and hydrofluoric acids using sodium hydroxide and sodium chloride (local mineral raw materials) to obtain fluoride salts – a mixture of silicofluoride and sodium fluoride. As a result of laboratory studies, the optimal parameters of the process of acid mixture processing using sodium hydroxide and sodium chloride to obtain a mixture of SSF and sodium fluoride were determined: temperature – 20-25 °C, duration – 10-20 min, concentration of sodium hydroxide and sodium chloride – 20-25%; in this case the maximum degree of extraction of SSF and sodium fluoride is achieved. The product obtained when sodium hydroxide was used under these optimum conditions was chemically analyzed and found to consist of 67.8% Na2SiF6 and 31.5% NaF. In the case of sodium chloride, the resulting mixture contains 95.8% of Na2SiF6 and 3.5% of NaF. To confirm the reliability of the results of chemical analysis and the found optimal parameters, as well as to determine the mineralogical composition of the obtained fluoride salts, X-ray phase analysis was carried out using an upgraded Dron-2 unit. The obtained product can be used in electrolysis production for obtaining aluminosilicon alloy (silumin) and electrolyte melt, as well as for fluoridation of drinking water and in the production of acid-resistant cements. On the basis of the conducted laboratory studies, the basic technological scheme of processing of the mixture of FSA and hydrofluoric acid with the use of sodium hydroxide and sodium chloride was developed.

Strategic Oversight and Quality Validation in Spice Production Enhancement

This study examines the effectiveness of a comprehensive control management and quality control system in the seasoning production process, focusing specifically on a menu-specific seasoning variant (MSS). The methodology encompassed various stages including material acceptance, sieving, mixing, and final packaging, integrated with stringent control measures such as equipment and facility cleaning, material separation, and machine maintenance. Quality control tests conducted included sodium chloride content, pH analysis, seal strength, and bubble tests. Results indicate that these systematic processes significantly enhance product quality by ensuring compliance with predefined quality standards. The implications of this study suggest that rigorous process oversight and detailed quality assessments can markedly improve the reliability and safety of food seasoning products, providing a framework for industry-wide best practices. Highlights: Process Management: Systematic control measures, including equipment and facility cleaning, ensure a contamination-free production environment. Quality Control: Rigorous testing, including sodium chloride content and pH analysis, guarantees that the products meet established quality and safety standards. Product Compliance: Implementation of advanced control and quality assurance protocols ensures that products consistently comply with industry standards. Keywords: Instant Seasoning, Quality Control, Safety Standards

A combination of omics-based analyses to elucidate the effect of NaCl concentrations on the metabolites and microbial dynamics during the ripening fermentation of Pixian-Douban

The study investigated the effect of different sodium chloride (NaCl) concentrations (10%, 15%, and 20%) on the ripening fermentation of Pixian-Douban, a traditional fermented condiment. The results showed that NaCl affected the dynamics of physicochemical parameters, volatile components, fatty acids, amino metabolites, organic acids, and microbial composition, and their dynamic modes were different. After 253 days fermentation, the 10% NaCl Pixian-Douban had significantly (p < 0.05) higher levels of total organic acids (20,308.25 mg/kg), amino metabolites (28,144.96 mg/kg), and volatiles (3.36 mg/kg) compared to 15% and 20% NaCl Pixian-Douban. Notably, the possible health risk associated with high concentration of biogenic amines in 10% NaCl Pixian-Douban is of concern. Moreover, correlation analyses indicated that the effect of NaCl on the quality of Pixian-Douban may be mainly related to bacteria. This study deepens the knowledge about the role of NaCl in ripening fermentation of Pixian-Douban and contributes to develop low-NaCl Pixian-Douban product.

Mitigation of Salinity Combined with Vermicompost on Growth, Antioxidant Enzymes and Macro Nutritional Changes in Solanum melongena L.

Background: The main abiotic stress affecting crop production and productivity is due to the salinity stress, which has a very serious adverse effectiveness on plant development and production. At higher concentrations, salt stress provokes the accumulation of reactive oxygen species ROS, which are toxic to cells. Methods: The goal of the current study was to determine how different sodium chloride (NaCl) concentrations affected Solanum melongena growth, antioxidant enzymes and macronutrients through the use of vermicompost. Vermicompost (VC) (%) (0, 10, 25, 50, 10 mM+5%, 25 mM+10%, 50 mM+25% and 100% VC) was used in the germination investigation and the data were evaluated 30- and 60-days following germination. Result: In 50 mM of NaCl plus 25% VC, the greatest increases in the length of the shoot, length of roots, both dry and fresh weight and leaf area were noted. On both days, the maximum enzyme activity was observed at 50 mM NaCl + 25% VC. The use of vermicompost in NaCl stress enhanced the nutritional contents up to 50 mM + 25% VC, while the accumulation of macronutrient content increased at an extreme level of 100% VC.

Controlling sodium chloride concentration modulates the supramolecular structure and sol features of wheat starch-acetylated starch binary matrix

The sol performance of wheat starch (WS) matrix incorporating acetylated starch (AS) is crucial for the processing and quality features of wheat products. From a supramolecular structure view, how regulating salt (sodium chloride) concentration modulates the sol features, e.g., pasting, zero-shear viscosity (ZSV) and thixotropy of WS-AS binary matrix was explored. Compared to the salt-free counterpart, the saline matrices exhibited a delayed pasting profile and a decreased viscoelasticity. Thereinto, the sol at 0.02 M NaCl exhibited the smallest ZSV (23,710 Pa·s) and the greatest in-shear recovery ratio (33.7 %). Such variations could be attributed to the weakened coil-helix, nematic-smectic and isotropy-anisotropy transitions from a side-chain liquid-crystalline perspective. Meanwhile, the correlation length (ξ) and radius of gyration (Rg) obtained from small angle X-ray scattering analysis were increased by 5.2 and 9.6 Å respectively, which disclosed a restrained entanglement and an enhanced chain mobility. These results would provide a reference for the design of fluid/semisolid products with optimized qualities.

Experimental evaluation of the protective properties of a humic preparation in relation to cultivated plants under salt stress

In connection with the widespread problem of soil salinity, the issue of increasing the salt tolerance of cultivated plants is becoming increasingly important. In this aspect, humic substances and preparations based on them are very promising. The aim of the study was to evaluate the effect of humic preparations on agricultural crops and lawn cereals under salt stress. Under laboratory conditions, the protective properties of the humic preparation «Ekorost» were evaluated in relation to the industrial crop Brassica rapa L. and to lawn grasses under the influence of salt stress induced by excessive concentrations of sodium chloride and anti-icing agent. It was revealed that the humic preparation «Ekorost» exhibits protective properties in relation to the indicated test cultures under conditions of artificially simulated salt stress, which is expressed in a noticeable stimulation of their germination and growth processes. In all the experiments, the most effective was a 0.1% aqueous solution of the drug «Ekorost», which allows us to recommend it in the indicated dosage to increase the salt tolerance of oil radish ( Brassica rapa L.) and lawn cereals.

Antibody desolvation with sodium chloride and acetonitrile generates bioactive protein nanoparticles.

About 30% of the FDA approved drugs in 2021 were protein-based therapeutics. However, therapeutic proteins can be unstable and rapidly eliminated from the blood, compared to conventional drugs. Furthermore, on-target but off-tumor protein binding can lead to off-tumor toxicity, lowering the maximum tolerated dose. Thus, for effective treatment therapeutic proteins often require continuous or frequent administration. To improve protein stability, delivery and release, proteins can be encapsulated inside drug delivery systems. These drug delivery systems protect the protein from degradation during (targeted) transport, prevent premature release and allow for long-term, sustained release. However, thus far achieving high protein loading in drug delivery systems remains challenging. Here, the use of protein desolvation with acetonitrile as an intermediate step to concentrate monoclonal antibodies for use in drug delivery systems is reported. Specifically, trastuzumab, daratumumab and atezolizumab were desolvated with high yield (∼90%) into protein nanoparticles below 100 nm with a low polydispersity index (<0.2). Their size could be controlled by the addition of low concentrations of sodium chloride between 0.5 and 2 mM. Protein particles could be redissolved in aqueous solutions and redissolved antibodies retained their binding activity as evaluated in cell binding assays and exemplified for trastuzumab in an ELISA.

Salinity tolerance and desalination properties of a Haematococcus lacustris strain from eastern Hungary.

Nowadays the increasing amount of saline wastewaters has given rise to various biological desalination processes, among which the application possibilities of microalgae represents a priority research area. Next to "real" aquatic species (members of phytoplankton or phytobenthon), species from ephemeral aquatic habitats or aeroterrestrial algae also could be good candidates of research studying salt tolerance or desalination ability, since salinity stress is often referred as "physiological drought" and species from ephemeral habitats can be characterized by high drought tolerance. In this study, the salinity tolerance, salt and nutrient removal ability of a Haematococcus lacustris strain from eastern Hungary were investigated. Vegetative cells showed low salt tolerance, survival was ensured by the formation of cysts up to a sodium-chloride concentration of 2,000 mg l-1. Although relatively moderate (a max. 30%) conductivity reduction and chloride removal were observed, notable (nearly 100%) nitrate and phosphate removal occurred even in the presence of 2,000 mg l-1 NaCl. Carotenoid accumulation was observed earlier and in higher extent in salt treated cultures than in drying out ones, although the amount of astaxanthin-esters was significantly higher in the cultures of drying out experiment than in the corresponding cultures of salt treatment characterized with similar chloride content. Our results suggest that algae isolates from ephemeral aquatic habitats endangered by regular drying out (exposed to special salt stress), could have notable salt tolerance and consequently successful applicability in nutrient removal processes from slightly saline wastewaters. The accumulation of valuable metabolites (such as astaxanthin) as a response to salinity stress, could enhance the economic value of the biomass.

Mechanical Stress Induces Sodium Entry and Osmoprotective Responses in Murine Synovial Fibroblasts.

Osteoarthritis (OA) is a multifactorial disease depending on molecular, genetic, and environmental factors like mechanical strain. Next to the cartilage and the subchondral bone, OA also affects the synovium, which is critically involved in the maintenance of joint homeostasis. As there is a correlation between the extracellular sodium content in the knee joint and OA, this study investigates the impact of sodium on OA-associated processes like inflammation and bone remodeling without and with mechanical loading in synovial fibroblasts. For that purpose, murine synovial fibroblasts from the knee joint were exposed to three different extracellular sodium chloride concentrations (-20 mM, ±0 mM and +50 mM NaCl) in the absence or presence of compressive or intermittent tensile strain. In addition to the intracellular Na+ content and gene expression of the osmoprotective transcription factor nuclear factor of activated T cells 5 (Nfat5), the gene and protein expression of inflammatory mediators (interleukin-6 (IL6), prostaglandin endoperoxide synthase-2 (Ptgs2)/prostaglandin E2 (PGE2)), and factors involved in bone metabolism (receptor activator of NF-κB ligand (RANKL), osteoprotegerin (OPG)) were analyzed by qPCR and ELISA. Mechanical strain already increased intracellular Na+ and Nfat5 gene expression at standard salt conditions to levels obtained by exposure to increased extracellular Na+ content. Both high salt and compressive strain resulted in elevated IL6 and PGE2 release. Intermittent tensile strain did not increase Il6 mRNA expression or IL6 protein secretion but triggered Ptgs2 expression and PGE2 production. Increased extracellular Na+ levels and compressive strain increased RANKL expression. In contrast, intermittent tension suppressed RANKL expression without this response being subject to modification by extracellular sodium availability. OPG expression was only induced by compressive strain. Changes in extracellular Na+ levels modified the inflammatory response and altered the expression of mediators involved in bone metabolism in cells exposed to mechanical strain. These findings indicate that Na+ balance and Nfat5 are important players in synovial fibroblast responses to mechanical stress. The integration of Na+ and Na+-dependent signaling will help to improve the understanding of the pathogenesis of osteoarthritis and could lead to the establishment of new therapeutic targets.

Controlling Clostridium sporogenes spoilage of “requeijão cremoso” processed cheese: Modeling the growth/no-growth probability as a function of pH, sodium chloride and nisin

Predictive models were developed to assess the growth response of Clostridium sporogenes in “requeijão cremoso” processed cheese, as affected by pH and sodium chloride and nisin concentrations. A complete factorial design was performed using different formulations: pH (5.2, 5.6, and 6.0), sodium chloride (0.5, 0.75, 1.0, 1.25, and 1.5%), and nisin (0.0, 5.0, 7.5, 10.0 and 12.5 mg/kg), totalizing 75 conditions. Packs with 150 g of “requeijão cremoso” were inoculated with 103 spores/g following storage at 10 °C for up to 60 days. The counts of C. sporogenes were performed on day zero and at the end of storage after a heat shock (80 °C for 12 min). The formulations that allowed an increase in the population >1 log CFU/g were assigned the value of 1 (growth), and the formulations in which the increase was <l log CFU/g were assigned 0 (no growth). Overall, the growth of C. sporogenes was affected by pH, salt concentration and nisin, e.g. at pH 5.2 the growth/no growth limit is 1.25% salt and no nisin, at pH 5.6 this limit is reduced to 0.75% salt, while at pH 6.0 only the effect of pH was sufficient to inhibit C. sporogenes even in the absence of salt and nisin. All isolated parameters and their combinations significantly affected spore germination, indicating that the model generated significantly contributes to the optimization of the processing and mitigation of the microbiological spoilage of “requeijão cremoso” processed cheese by Clostridia.

Physicochemical and Sensory Characterization of Whey Protein-Enriched Semihard Cheese.

In view of potential future changes of German food legislation with regard to cheese product quality parameters, this study aimed to evaluate the quality of whey protein-enriched semihard cheese (WPEC). Model WPEC was produced in a pilot plant and on an industrial scale by adding defined amounts of high-heat (HH) milk to the cheese milk and comprehensively analyzed during cheese processing. The dry matter, total protein, pure protein, fat, and sodium chloride content of six-week ripened cheese samples were not significantly different (p < 0.05) when the technologically necessary heating of the curd was adapted to the amount of HH milk. However, the ripening, firmness, and melting behavior of WPEC was different compared to cheese without HH milk. During ripening, no formation of whey protein peptides was observed, but differences in the amount of some bitter peptides deriving from the casein fraction were found. Sensory data suggested a slightly more bitter taste perception by the panelists for the WPEC. Further technological adjustments are recommended to obtain marketable WPEC.

Investigation of the liquid water content in composite saline soil containing chloride and sulfate ions

Different salt types have different effects on the liquid water content of saline soil, resulting in differences in the physico-mechanical properties and water/salt migration process of saline soil. In order to investigate the phase transition process and the change of the liquid water content in composite saline soil, saline soils with the same total salt content and different ratios of sodium chloride and sodium sulfate were taken as the objects. The results indicated that two phase transitions occur in the saline soil with single salt type, while three phase transitions can be found in the composite saline soil with two salt types. Mirabilite crystallization contributes to the 1st phase transition, mirabilite and ice precipitate together in the 2nd phase transition process, and mirabilite, ice, and hydrohalite precipitate simultaneously in the 3rd phase transition process. The liquid water is reduced in the phase transition process during cooling, and the pore characteristic has been changed significantly. The change of the liquid water content reflects the processes of salt crystallization and ice formation in saline soil, then the amounts of ice and hydrated salt were calculated at different temperatures, and the mechanism of inhibiting the deformation of sulfate saline soil was examined by adding sodium chloride. The results have reference value for those seeking understanding of the deformation in natural composite saline soil, and these findings can provide theoretical basis for the phase transition mechanism of saline soil in cold regions.

Impact of Ionic Strength (Sodium Chloride Concentration) on Homopolymerization and Copolymerization Kinetics of Acrylamide and 2‐Acrylamido‐2‐Methylpropane Sulfonic Acid

AbstractAnionic polyelectrolytes can be used for a variety of applications, including flocculation and enhanced oil recovery. While it is widely recognized that polyelectrolyte synthesis is impacted by the pre‐polymer formulation and polymerization conditions, the specific relationships between these factors and the subsequent polymer properties are not well understood. Therefore, the current work intends to improve understanding of ionic strength (IS) effects during the copolymerization of 2‐acrylamido‐2‐methylpropane sulfonic acid (AMPS) and acrylamide (AAm). The aims of the study are i) to use in situ H1 NMR to study copolymerization kinetics, and ii) to determine how increasing IS impacts copolymerization kinetics (and, by extension copolymer microstructure). It is found that altering IS prior to copolymerization has significant impacts on the reactivity ratios, and therefore impacts the microstructure through multiple mechanisms. Increasing IS causes a crowding effect, where the propagating chain develops a random coil conformation and causes steric hindrance of the large AMPS monomer, decreasing the likelihood of AMPS propagation. When the IS is increased further, the ionic shielding effect is more impactful, increasing the likelihood of AMPS propagation. Ultimately, this work will make it possible to manipulate IS to synthesize AMPS/AAm copolymers with specific desirable properties for target applications.

Polymers in Drug Delivery: Analyzing Beta-carotene Aggregation in Response to PEG-b-PLA

Synthetic polymers have quickly become ubiquitous in all aspects of modern society, from single-use plastics to medical treatment. This study focuses on the behavior of polymers in the medical context of drug delivery, which requires a highly controlled and targeted release of the therapeutic agent. However, current medical treatments such as anticancer drugs lack precision and lose effectiveness after entering the body. PEG-b-PLA is uniquely structured to have both hydrophilic and hydrophobic ends, which makes it capable of forming polymeric micelles. Beta-carotene, a lipophilic compound, was employed as a surrogate molecule to represent an anticancer drug. This study’s purpose was to study PEG-b-PLA in aqueous solutions with varying concentrations of Beta-carotene and sodium chloride to mimic a human drug delivery procedure. It was hypothesized that PEG-b-PLA would limit the aggregation of Beta-carotene to a constant size, regardless of the outside environment. For that purpose, this study created four mixtures, each with varying concentrations of DI water, Beta-carotene, sodium chloride, and PEG-b-PLA. Qualitative data was collected by using a laser beam to observe the aggregation of Beta-carotene in each mixture, and each mixture was analyzed in a dynamic light scattering (DLS) instrument to determine exact micelle sizes. It was determined that the addition of PEG-b-PLA significantly reduced the aggregation of Beta-carotene in an electrolyte solution, demonstrating the stabilizing role PEG-b-PLA plays in drug delivery. Recent innovations like smart polymers consist of molecules like PEG-b-PLA and have been shown to successfully transport medical drugs, potentially making highly targeted anticancer treatment a reality.

Sodium lignosulfonate doped polyvinyl alcohol-based hydrogel conductive composites based on ion-specific effects with continuously tunable mechanical properties and excellent conductive sensing properties

Hydrogels have great application prospects in the fields of bionics, soft tissue probes, and motion detection, etc. However, the high water content and loose cross-linker structure of hydrogels lead to weak mechanical properties, which limits their application and development. Therefore, in this study, sodium lignosulfonate doped polyvinyl alcohol hydrogels (PVA/LS) were constructed using polyvinyl alcohol as the matrix and sodium lignosulfonate as the filler; the hydrogels were also treated with different ionic solutions to construct ion-responsive hydrogels (PVA/LS/ION); and ion-conducting hydrogels (PVA/LS/CL) were constructed by the change of the concentration of sodium chloride (NaCl) solution. The hydrophilicity of the polyvinyl alcohol (PVA) molecular chain was changed by different ions, resulting in either water absorption (salting in) or dehydration (salting out), and the hydrogel mechanical properties could be adjusted over a wide range (tensile strength: 77 kPa ∼ 3.26 MPa; elongation at break: 290 ∼ 870%; modulus: 24 ∼ 563 kPa; toughness: 96 kJ/m3 ∼ 14.1 MJ/m3). In addition, the hydrogels have good conductivity regulation (1.09 ∼ 2.72 S/m) and sensing properties, with GF values up to 1.23, and sensitive and stable strain sensing properties. This work provides useful insights into the application of ion-specific effects to the field of hydrogels.

Germination responses of the invasive hedge cactus (Cereus uruguayanus) to environmental factors

AbstractHedge cactus (Cereus uruguayanus R. Kiesling; syn.: Cereus hildmannianus K. Schum.) is a columnar cactus that was introduced to Australia as an ornamental plant and has since become invasive in subhumid regions of Queensland and New South Wales. Compared with its congener, queen of the night (Cereus jamacaru DC.), which is currently invasive in both eastern and southern Africa, information on seed biology of C. uruguayanus is lacking. Experiments were conducted to study the effects of alternating day/night temperature, salt stress, water stress, and burial depth on germination and seedling emergence of four seed accessions of C. uruguayanus. Seeds were also subjected to a controlled aging test (CAT) to obtain an estimate of potential persistence under field conditions. The optimum temperature regime for germination of all accessions was 30/20 C. Germination decreased with an increase in sodium chloride (NaCl) concentration, but germination of all accessions (range 26% to 81%) occurred at 160 mM NaCl, indicating very high salt tolerance. Seed germination gradually decreased with an increase in water stress, but germination in all accessions (range 19% to 47%) occurred at −0.8 MPa. Seed viability and dormancy status were unaffected by exposure to salt level (320 mM NaCl) and water (−1.6 MPa) stress under which germination did not occur. Germination responses to all three factors were generally similar to those documented for C. jamacaru. The emergence of C. uruguayanus decreased with an increase in seed burial depth. The highest emergence (43%) was recorded for surface-sown seeds, and emergence was reduced to 0 at a burial depth of 2 cm. CAT results for two seed accessions indicated that seeds of C. uruguayanus are likely to demonstrate extended (&gt;3 yr) persistence under field conditions, a prediction that is supported by evidence that germination of its small (2-mm) seeds is markedly reduced by burial.