Superoxide Dismutase Mimetic Activity Research Articles

SOD mimetic activity of salicylatocopper complexes‡

Investigation of salicylatocopper complexes in the presence of a nitrogen donor ligand is a growing research area due to the interesting mimetic activities of such complexes. Here, three X-salicylatocopper (where X = 3-methyl, or 4-methoxy) complexes with three different

Synthetic Methods Over Crystal Structures of Two Copper(II) Coordination Complexes Based on Pyrazine Derivatived Ligand

Two novel copper(II) coordination polymers, [Cu2(pypymba)3]n (1) and [Cu2(pypymba)4]n (2) (H pypymba = 4-((3-(pyrazin-2-yl)-1H-pyrazol-1-yl)methyl)benzoic acid), have been synthesized with copper nitrate and pyrazinyl ligand by hierarchical diffusion method and hydrothermal method respectively, and characterized by the element analysis, IR, TGA and single crystal X-ray diffraction. The results of structural analysis indicate that 1 crystallizes in monoclinic form with P21/c space group, while 2 crystallizes in the triclinic form with Pī space group. Furthermore, 1 has a binuclear unit, in which both of the two central Cu(II) atoms adopt a six-coordination mode but exist different coordination environments, and forms 1D helix structure by Cu(II)–O coordination bond. However, 2 is a mononuclear unit with a six-coordinated mode similar to 1 and the 1D loop-chain is formed via C–H···O hydrogen bonding interaction. In addition, superoxide dismutase (SOD) mimetic activities of 1 and 2 have been investigated by the modified Marklund method and it reveals both of the two compounds show certain SOD-like activities. Two novel coordination compounds [Cu2(pypymba)3]n (1) and [Cu2(pypymba)2]n (2) (H pypymba = 4-((3-(pyrazin-2-yl)-1H-pyrazol-1-yl)methyl)benzoic acid) were synthesized with copper nitrate and Hpypymba ligand by hierarchical diffusion method and hydrothermal method respectively and characterized through single-crystal X-ray diffraction. Complex 1 crystallized in the monoclinic form with P21/c space group while 2 crystallized in the triclinic form with Pī space group. Since the only difference in the synthetic conditions between complexes 1 and 2 is that of the synthetic methods, resulting in the topologies and behavior of the two complexes being significantly distinct. It is clear that the choice of synthetic methods plays a key role in controlling the structure of the coordination compounds.

SOD mimetic activity and antiproliferative properties of a novel tetra nuclear copper (II) complex.

The search for novel anticancer therapeutic agents is an urgent and important issue in medicinal chemistry. Here, we report on the biological activity of the copper-based bioinorganic complex Cu4 (2,4-di-tert-butyl-6-(1H-imidazo- [1, 10] phenanthrolin-2-yl)phenol)4]·10 CH3CN (2), which was tested in rat L6 myotubes, mouse NSC-34 motor neurone-like cells, and HepG-2 human liver carcinoma. Upon 96h incubation, 2 exhibited a significant cytotoxic effect on all three types of cells via activation of two cell death mechanisms (apoptosis and necrosis). Complex 2 exhibited better potency and efficacy than the canonical cytotoxic drug cisplatin. Moreover, during shorter incubations, complex 2 demonstrated a significant SOD mimetic activity, and it was more effective and more potent than the well-known SOD mimetic TEMPOL. In addition, complex 2 was able to interact with DNA and, cleave DNA in the presence of sodium ascorbate. This study shows the potential of using polynuclear redox active compounds for developing novel anticancer drugs through SOD-mimetic redox pathways.

Enzyme-mimetic activity of Ce-intercalated titanate nanosheets.

Colloidal solutions of Ce-doped titanate nanosheets (Ce-TNS) with tiny dimensions (<10 nm) were fabricated through a hydrolysis reaction of titanium tetraisopropoxide and Ce(NO3)3, and their annihilation activity for reactive oxygen species (ROS) was investigated. The obtained Ce-TNS had an akin crystal structure to layered tetratitanate (Ti4O9(2-)) and Ce ions occupied interlayer space between the host layers with a negative charge. The Ce-TNS possessed a superoxide dismutase (SOD) mimetic activity for disproportionation of superoxide anion radicals (O2(-)) as target ROS. It was explained that the annihilation of O2(-) caused a valence fluctuation of Ce ions existing in the interlayer. Moreover, the activity of Ce-TNS exceeded that of CeO2 nanoparticles recently attracting much attention as an inorganic SOD mimic. The superior performance was explained mainly by a high dispersion stability of the Ce-TNS bringing about a huge reaction area. Moreover, the Ce-TNS protected DNA molecules from ultraviolet light induced oxidative damage, demonstrating effectiveness as one of the new inorganic protecting agents for biomolecules and tissues.

Studies on metal complexes of 1-((8-hydroxyquinolin-5-yl)methyl)-3-(thiazol-2-ylimino)indolin-2-one

Novel ligand 1-((8-hydroxyquinolin-5-yl)methyl)-3-(thiazol-2-ylimino)indolin-2-one was synthesized by condensation of 5-(chloromethyl)-1,8a-dihydroquinolin-8-ol with 3-(thiazol-2-ylimino)indolin-2-one. Metal complexes of this ligand were prepared using various transition metal ion salts, viz., Mn(II), Fe(II), Co(II), Ni(II), Cu(II), and Zn(II). All synthesized compounds were characterized by various spectroscopic techniques. Thermogravimetric analysis of metal complexes was also carried out. Geometry of the metal complexes was carried out by electronic spectral analysis and magnetic measurement studies. The ligand and metal complexes were screened for their antibacterial activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris, and Pseudomonas aeruginosa. The metal complexes are found more potent bactericides than the ligand. The interaction of ligand and its complexes with plasmid (pUC 19) DNA and calf thymus DNA was investigated by gel electrophoresis measurements. The superoxide dismutase mimetic activity of the ligand and its metal complexes has also been assessed for their ability to inhibit the reduction of nitrobluetetrazolium chloride.

The protective effects of trace elements against side effects induced by ionizing radiation.

Trace elements play crucial role in the maintenance of genome stability in the cells. Many endogenous defense enzymes are containing trace elements such as superoxide dismutase and metalloproteins. These enzymes are contributing in the detoxification of reactive oxidative species (ROS) induced by ionizing radiation in the cells. Zinc, copper, manganese, and selenium are main trace elements that have protective roles against radiation-induced DNA damages. Trace elements in the free salt forms have protective effect against cell toxicity induced by oxidative stress, metal-complex are more active in the attenuation of ROS particularly through superoxide dismutase mimetic activity. Manganese-complexes in protection of normal cell against radiation without any protective effect on cancer cells are more interesting compounds in this topic. The aim of this paper to review the role of trace elements in protection cells against genotoxicity and side effects induced by ionizing radiation.

Can nitroxides evoke the Keap1–Nrf2–ARE pathway in skin?

Nitroxides are stable cyclic radicals of diverse size, charge, and lipophilicity. They are cell-permeative, which effectively protects cells, tissues, isolated organs, and laboratory animals from radical-induced damage. The mechanisms of activity through which nitroxides operate are diverse, including superoxide dismutase-mimetic activity, oxidation of semiquinone radicals, oxidation of reduced metal ions, procatalase-mimetic activity, interruption of radical chain reactions, and indirect modulation of NO levels. Nitroxides possess both a nucleophilic (reducing properties) and an electrophilic (oxidizing properties) nature and, therefore, they may affect different cellular pathways. In the current study, a novel mechanism of action by which nitroxides provide skin protection based on their electrophilic nature is suggested. This study shows that nitroxides may act as electrophiles, directly or indirectly, capable of activating the Keap1–Nrf2–ARE pathway in human keratinocytes (HaCaT) and in human skin (human organ culture model). The high potency of oxoammonium cations versus hydroxylamines in activating the system is demonstrated. The mechanism of action by which nitroxides activate the Keap1–Nrf2–ARE pathway is discussed. Understanding the mechanism of activity may expand the usage of nitroxides as a skin protection strategy against oxidative stress-related conditions.

Synthesis, characterization, antibacterial, DNA cleavage and SOD activities of some 3d metal complexes of ethyl[2-(1H-indol-3-yl)methyleneamino]-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylate

A series of Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes have been synthesized using a novel Schiff base ligand derived from indole-3-carboxaldehyde and 2-amino-3-carboxyethyl-4,5,6,7-tetrahydrobenzo[b]thiophene. The ligand and the metal complexes have been characterized on the basis of various physico-chemical and spectral studies. The analytical, spectral data (Infrared, UV–Vis, 1H NMR, 13C NMR, ESR, FAB mass) and magnetic susceptibility measurements indicate that the ligand is coordinated to the metal ion through azomethine nitrogen and oxygen of the ester carbonyl group. The ligand and the metal complexes have been screened for their antibacterial activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa. The results indicate that the metal complexes are more potent bactericides than the ligand. DNA cleavage activity of the ligand and the metal complexes has been examined by agarose gel electrophoresis technique. The copper(II) complex shows increased nuclease activity when compared to the ligand and the metal complexes. Superoxide dismutase (SOD) mimetic activity of the ligand and complexes has also been assessed for their ability to inhibit the reduction of nitrobluetetrazolium chloride (NBT), and it is observed that they possess promising SOD mimetic activity.

Synthesis, structure, antioxidant and SOD-mimetic activity of [Cu(xanthurenate)(nicotinamide)(H2O)] complexes

This study reports the synthesis of novel mixed ligand complexes of copper(II) with the xanthurenate anion (Xan2−) and nicotinamide ligand (NA): [Cu(Xan)(NA)(H2O)]·H2O and [Cu(Xan)(NA)(H2O)]·3H2O. The compounds were characterized by single crystal X-ray diffraction, IR spectroscopy, and thermal analysis. The first coordination sphere of copper(II) in [Cu(Xan)(NA)(H2O)] consists of a water molecule, a nicotinamide ligand coordinated via the pyridine nitrogen and a xanthurenate dianion coordinated in a tridentate manner via carboxylate oxygen, nitrogen, and phenolate oxygen. The five donor atoms define a distorted square pyramid whose apex is occupied by the aqua ligand. The antioxidant activities of the compounds were investigated by the 2,2-diphenyl-1-picrylhydrazyl scavenging method, which showed that the IC 50 values for both compounds are higher than for related [Cu(Xan)(H2O)2] complex. The title compounds also exhibit superoxide dismutase mimetic activity.

PEGylated nanoceria protect human epidermal cells from reactive oxygen species

Background Cerium oxide nanoparticles (CeNPs) have shown promise as catalytic antioxidants in cell culture and animal models due to its superoxide dismutase and catalase mimetic activities. CeNPs can exist in +3 and +4 oxidation states, which have been suggested as the mechanism behind the free radical scavenging activity. It has also been shown that unique crystal structure of CeNPs with surface oxygen defects promote the shuffling between the +3 and +4 oxidation states that help in eliminating the free radicals. This activity of CeNPs has been suggested as the mechanism, at least in part, behind increase in cellular longevity and decrease in the toxic insults in mammalian cells/tissues. Further, the uniform distribution of CeNPs, upon cellular uptake, within the cells could prevent the accumulation of reactive oxygen species in the cells.

Synthesis, spectroscopic characterisation, DNA cleavage, superoxidase dismutase activity and antibacterial properties of some transition metal complexes of a novel bidentate Schiff base derived from isatin and 2-aminopyrimidine

Complexes of manganese(II), cobalt(II), nickel(II), copper(II) and zinc(II) with a Schiff base, formed by the condensation of isatin with 2-aminopyrimidine have been synthesised and characterised through elemental analysis, molar conductance measurements, magnetic susceptibility, IR, UV–Vis, 1HNMR, FAB mass and EPR spectral studies. The spectral data revealed that the ligand acts as neutral bidentate, coordinating to the metal ion through the carbonyl oxygen and azomethine nitrogen. Molar conductance values adequately support the electrolytic nature of the complexes. On the basis of the above observations the complexes have been formulated as [M(ISAP)2]X2, where M=Mn(II), Co(II), Ni(II), Cu(II) and Zn(II); X=Cl, OAc; ISAP=2-[N-indole-2-one]aminopyrimidine. The ligand and copper(II) complex were subjected to X-ray diffraction studies. The DNA cleavage study was monitored by gel electrophoresis method. The superoxide dismutase (SOD) mimetic activities of the ligand and the metal complexes were checked using NBT assay. The in vitro antibacterial activity of the synthesized compounds has been tested against gram negative and gram positive bacteria.

Superior Therapeutic Index of Calmangafodipir in Comparison to Mangafodipir as a Chemotherapy Adjunct

Mangafodipir is a magnetic resonance imaging contrast agent with manganese superoxide dismutase (MnSOD) mimetic activity. The MnSOD mimetic activity protects healthy cells against oxidative stress-induced detrimental effects, e.g., myelosuppressive effects of chemotherapy drugs. The contrast property depends on in vivo dissociation of Mn2+ from mangafodipir—about 80% dissociates after injection. The SOD mimetic activity, however, depends on the intact Mn complex. Complexed Mn2+ is readily excreted in the urine, whereas dissociated Mn2+ is excreted slowly via the biliary route. Mn is an essential but also a potentially neurotoxic metal. For more frequent therapeutic use, neurotoxicity due to Mn accumulation in the brain may represent a serious problem. Replacement of 4/[5] of Mn2+ in mangafodipir with Ca2+ (resulting in calmangafodipir) stabilizes it from releasing Mn2+ after administration, which roughly doubles renal excretion of Mn. A considerable part of Mn2+ release from mangafodipir is governed by the presence of a limited amount of plasma zinc (Zn2+). Zn2+ has roughly 103 and 109 times higher affinity than Mn2+ and Ca2+, respectively, for fodipir. Replacement of 80% of Mn2+ with Ca2+ is enough for binding a considerable amount of the readily available plasma Zn2+, resulting in considerably less Mn2+ release and retention in the brain and other organs. At equivalent Mn2+ doses, calmangafodipir was significantly more efficacious than mangafodipir to protect BALB/c mice against myelosuppressive effects of the chemotherapy drug oxaliplatin. Calmangafodipir did not interfere negatively with the antitumor activity of oxaliplatin in CT2[6] tumor-bearing syngenic BALB/c mice, contrary calmangafodipir increased the antitumor activity.

Mixed-ligand Cu(II)–vanillin Schiff base complexes; effect of coligands on their DNA binding, DNA cleavage, SOD mimetic and anticancer activity

SOD mimics with varying coligand are momentous in developing potential chemotherapeutic drugs. Cu(II) based SOD mimics 1–4 [CuLH(OAc)(H2O)Y)] (LH = 2-((E)-(1,3-dihydroxy-2-methylpropan-2-ylimino)methyl)-6-methoxyphenol, OAc = CH3COO, 1: Y = H2O; 2: Y = phen (1,10-phenanthroline), 3: Y = tpimH (2,4,5-triphenylimidazole); 4: Y = tfbimH (2-(trifluoromethyl)benzimidazole) were synthesized and thoroughly characterized. Their interaction with CT-DNA showed different non-covalent binding behaviour. SOD activity of 2 was highest among 1–4 which was further validated by gel electrophoresis. The pBR322 plasmid strand break offered by 2 + O2·− system reveals oxidative cleavage mechanism. In vitro antimicrobial activity of 1–4 was shown by percent inhibition data while in vitro anticancer activity of 1–4 was screened using 16 human carcinoma cell lines of different histological origin. Complex 2 showed higher efficacy towards 14 cell lines.

Effects of MnDPDP and ICRF-187 on Doxorubicin-Induced Cardiotoxicity and Anticancer Activity

Oxidative stress participates in doxorubicin (Dx)-induced cardiotoxicity. The metal complex MnDPDP and its metabolite MnPLED possess SOD-mimetic activity, DPDP and PLED have, in addition, high affinity for iron. Mice were injected intravenously with MnDPDP, DPDP, or dexrazoxane (ICRF-187). Thirty minutes later, mice were killed, the left atria were hung in organ baths and electrically stimulated, saline or Dx was added, and the contractility was measured for 60 minutes. In parallel experiments, 10 µM MnDPDP or MnPLED was added directly into the organ bath. The effect of MnDPDP on antitumor activity of Dx against two human tumor xenografts (MX-1 and A2780) was investigated. The in vitro cytotoxic activity was studied by co-incubating A2780 cells with MnDPDP, DPDP, and/or Dx. Dx caused a marked reduction in contractile force. In vivo treatment with MnDPDP and ICRF-187 attenuated the negative effect of Dx. When added directly into the bath, MnDPDP did not protect, whereas MnPLED attenuated the Dx effect by approximately 50%. MnDPDP or ICRF-187 did not interfere negatively with the anti-tumor activity of Dx, either in vivo or in vitro. Micromolar concentrations of DPDP but not MnDPDP displayed an in vitro cytotoxic activity against A2780 cells. The present results show that MnDPDP, after being metabolized to MnPLED, protects against acute Dx cardiotoxicity. Both in vivo and in vitro experiments show that cardioprotection takes place without interfering negatively with the anticancer activity of Dx. Furthermore, the results suggest that the previously described cytotoxic in vivo activity of MnDPDP is an inherent property of DPDP.

The influence of agglomeration of nanoparticles on their superoxide dismutase-mimetic activity

Three types of colloidal systems based on CeO2−x, GdYVO4:Eu3+ and CePO4:Tb3+ are compared in this work. Nanoparticles with diameter 1.9±0.3nm were stabilized by sodium citrate. Ultramicroheterogeneous dispersion in water was found to be a typical hydrophobic colloidal system with negatively charged particles surface. CeO2−x colloidal solutions were chosen as a model system. Using coagulation by inorganic electrolytes, it was shown, that both individual nanoparticles and their agglomerates, which remain in a dynamic equilibrium of agglomerization-peptization, can be present in solutions. The reaction of the autoxidation of epinephrine to adrenochrome was used in order to determine the ability of nanoparticles to inactivate superoxide radicals. Superoxide dismutase (SOD) activity of CeO2−x nanoparticles increases during the decay of agglomerates in solution. Nanoparticles GdYVO4: Eu3+ as well as CeO2−x exhibit SOD mimetic activity.

Macrocyclic nickel(II) complexes: Synthesis, characterization, superoxide scavenging activity and DNA-binding

Abstract A new series of nickel(II) complexes with the tetraaza macrocyclic ligand have been synthesized as possible functional models for nickel–superoxide dismutase enzyme. The reaction of 5-amino-3-methyl-1-phenylpyrazole-4-carbaldehyde (AMPC) with itself in the presence of nickel(II) ion yields, the new macrocyclic cationic complex, [NiL(NO 3 ) 2 ], containing a ligand composed of the self-condensed AMPC (4 mol) bound to a single nickel(II) ion. A series of metathetical reactions have led to the isolation of a number of newly complexes of the types [NiL]X 2 ; X = ClO 4 and BF 4 , [NiLX 2 ], X = Cl and Br ( Scheme 1 ). Structures and characterizations of these complexes were achieved by several physicochemical methods namely, elemental analysis, magnetic moment, conductivity, and spectral (IR and UV–Vis) measurements. The electrochemical properties and thermal behaviors of these chelates were investigated by using cyclic voltammetry and thermogravimetric analysis (TGA and DTG) techniques. A distorted octahedral stereochemistry has been proposed for the six-coordinate nitrato, and halogeno complexes. For the four-coordinate, perchlorate and fluoroborate, complex species a square-planar geometry is proposed. The measured superoxide dismutase mimetic activities of the complexes indicated that they are potent NiSOD mimics and their activities are compared with those obtained previously for nickel(II) complexes. The probable mechanistic implications of the catalytic dismutation of O 2 - by the synthesized nickel(II) complexes are discussed. The DNA-binding properties of representative complexes [NiLCl 2 ] and [NiL](PF 4 ) 2 have been investigated by the electronic absorption and fluorescence measurements. The results obtained suggest that these complexes bind to DNA via an intercalation binding mode and the binding affinity for DNA follows the order: [NiLCl 2 ] □ [NiL](PF 4 ) 2 .

Therapeutic properties, SOD and catecholase mimetic activities of novel ternary copper(II) complexes of the anti-inflammatory drug Fenoprofen with imidazole and caffeine

The copper(II) ternary complexes of the non-steroidal anti-inflammatory drug Fenoprofen (Hfen) and the biologically relevant molecules imidazole (im) and caffeine (caf) as auxiliary ligands were investigated as novel anti-inflammatory agents. The new copper(II) complexes with formula [Cu(fen)2(im)2] (1) and Cu2(fen)4(caf)2 (2) were synthesized from the dinuclear complex [Cu2(fen)4(dmf)2] and characterized by IR, UV–Vis, EPR spectral and elemental analysis. The molecular structure of complex 1 was determined by X-ray crystallography. Both complexes 1 and 2 present enhanced and prolongued anti-inflammatory properties against the parent drug calcium Fenoprofenate, Ca(fen)2·2H2O, with a better performance for complex 1. Ternary complexes are potential models for several mono and poly-nuclear metal enzymes. The measured superoxide dismutase (SOD) mimetic activities of the complexes indicated a higher SOD mimic activity for complex 2 (IC50 of 0.24μM) than complex 1 (IC50 of 0.70μM), and also than the native enzyme evaluated by the same method (IC50 of 0.480μM). The catecholase activity of the complexes toward the aerobic oxidation of 3,5-di-tert-butylcatechol (dtbc) onto 3,5-di-tert-butylquinone (dtbq) showed that both complexes have moderate catalytic oxidase activity.

Antihyperglycemic and neuroprotective effects of one novel Cu–Zn SOD mimetic

Increasing evidence supports that OS plays important roles in diabetes mellitus and cerebral ischemia. This suggests that recovering an impaired endogenous superoxide dismutase (SOD) enzyme system induced by OS with a mimetic would be beneficial and protective for these diseases. In present study, one nonpeptidyl small molecular weight compound (D34) was synthesized. Its SOD mimetic activity and the potential therapeutic actions were also evaluated both in vivo and in vitro. The in vitro nitro blue tetrazolium (NBT) assay indicated that D34 presents an SOD mimetic activity. D34 (20μmol/kg) exhibited significant antihyperglycemic activity in alloxan-diabetic mice. D34 could also ameliorate the cerebral neuronal death in hippocampus of global cerebral ischemia mice. Furthermore, the D34 treatment significantly decreased malondialdehyde (MDA) contents and increased SOD activities in brains or livers of diabetes mice or cerebral ischemic mice. In conclusion, these preliminary findings support that D34 exhibits SOD mimetic activity and possesses significant antihyperglycemic and neuroprotective effects.

Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin

The bright chemiluminescence has been observed in the system: Co(2+)/hydrogen peroxide/lucigenin. The chemiluminescence intensity was directly proportional to either cobalt, hydrogen peroxide, or lucigenin concentrations. A procedure of determination of superoxide dismutase (SOD) activity by the chemiluminescence method in the cobalt-hydrogen peroxide-lucigenin system at pH 8.5 is suggested. A linear dependence was established between a relative chemiluminescence intensity and SOD concentration in the range of SOD concentrations between 0 and 4.5 nM, c (1/2) = 0.8 nM. The determination of SOD activity was performed in several tissue samples (rat plasma, erythrocyte hemolysate, and liver mitochondria). A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics.

Synthesis, characterization and antimicrobial activities of copper complexes derived from 4-aminoantipyrine derivatives

Cu(II) complexes have been synthesized from the Schiff base ligands derived from furfurlyidene-4-aminoantipyrine and aniline (L1)/p-nitroaniline (L2)/p-hydroxyaniline (L3). They were characterized using analytical and spectral techniques. All the Cu(II) complexes exhibit square planar geometry. The X-band ESR spectra of the copper complexes in DMSO solution at 300 and 77K were recorded and their salient features are reported. The in vitro biological screening effects of the investigated compounds were tested against the bacterial species, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa and fungal species, Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans by serial dilution method. A comparative study of inhibition values of the Schiff base ligands and their complexes indicate that the complexes exhibit higher antimicrobial activity than the Schiff base ligands. Superoxide dismutase and reducing power activities of the copper complexes have also been studied. Depending on the molecular structure, the [CuL2(OAc)2] complex possess promising SOD mimetic activities.