Abstract Introduction Research is the most reliable strategy to advance scientific understanding and enhance multiple healthcare services1. Integrating undergraduate health science students into research at an early stage is an important step towards improving critical thinking and problem-based solving, which in return enhances patient health outcomes2. Aim The aim of this study was to assess undergraduate health science students’ knowledge, attitudes, practices and perceived barriers towards research at Kuwait University. Methods A cross-sectional study was conducted among undergraduate health science students from the Colleges of Pharmacy, Medicine, Allied Health, Dentistry, and Public Health at Kuwait University. A self-administered online questionnaire consisting of five sections was used. The first section included questions to assess the demographic characteristics. The second section aimed to assess the knowledge of undergraduate students regarding basic research (score = low ≤3, moderate 4-5, high >5). The third section assessed attitude toward research by a five-point Likert scale (score = negative 31-75, neutral 76-110, positive 111-155). The fourth section of the questionnaire aimed to assess the perception of the barriers toward research practice (score = low 0-50, moderate 51-75, high 76-100). Finally, a section with eight questions on research practices. The questionnaire was distributed through online educational and social media platforms, such as WhatsApp and Microsoft Teams. Students from any academic year and who were enrolled as health science students at the time of the study were eligible to participate. The questionnaire was pilot tested prior to distribution. The recommended sample size was calculated using Raosoft (344 students)3. Ethical approval was received from The Health Sciences Ethical Committee, Kuwait University (reference: VDR/EC-393). Results A total of 364 undergraduate medicine, dentistry, pharmacy, allied health and public health students participated in the study. The participants were from different academic stages, ranging from first-year to seventh-year. The results showed that the students possessed moderate knowledge levels, with a median of 3 out of 8 according to the scoring system of the study. Most students had neutral attitudes (N=253; 69.5%) and moderate levels of perceived barriers (N=200; 54.9%). Medical and pharmacy students had significantly higher knowledge levels compared to the others (p<0.001; Kruskal-Wallis). The majority of students agreed that the lack of time is a barrier towards conducting research (N=237; 65.1%). Almost one half of the study participants took part in research projects (N=175; 48.1%) and a promising number of students had publications or poster presentations (N=67; 18.4%). Discussion/Conclusion The results suggest that undergraduate health science students at Kuwait University possess moderate research knowledge and have neutral attitudes towards research. Time constraints were the most frequently reported barrier to participation. While nearly half of the students participated in research projects, implementing mandatory research projects and providing research methodology workshops could significantly enhance student knowledge, attitudes, and future research engagement. A key limitation in this study is that observed relationships may not reflect long-term shifts in knowledge, attitudes, or practices. The study was also conducted in one university (Kuwait University), therefore the findings and results cannot be generalized to other undergraduate students.