Energy management strategy (EMS) has a great impact on securing fuel cell durability, battery charge sustenance, and fuel saving in fuel cell hybrid electric vehicles (FCHEVs). This study aims to develop EMS that can be applied in real-time to satisfy above conditions. Real time power separation was performed using rule-based EMS. A genetic algorithm (GA) was implemented to calculate the optimal battery charge/discharge criterion that simultaneously satisfies the minimum hydrogen consumption rate, battery charge rate preservation, and high fuel cell efficiency. The battery charge/discharge parameter values vary according to driving patterns, and in this paper, typical suburban, urban, and highway driving conditions are considered. For the real-time application of this research method, the effectiveness was demonstrated by applying the driving conditions of unknown patterns. The effect on the initial battery SOC on EMS was analyzed, and in order to verify the superiority of this method, it was compared and analyzed with EMS results using dynamic programming and fuzzy logic under the same driving cycles. The effectiveness of this research method was verified through simulation, and it was confirmed through experiments for real-time application. Since there is a limit to the experiment using an actual fuel cell vehicle, the experiment was performed using a fuel cell and battery. This method can be applied to real fuel cell vehicles in the same way.