Snake Scales Research Articles

Japanese monkeys rapidly noticed snake-scale cladded salamanders, similar to detecting snakes

The ability to detect threats quickly is crucial for survival. Primates, including humans, have been shown to identify snakes quickly and accurately due to their evolutionary history. However, it is unclear which visual features humans and primates detect as threat targets. Several studies have suggested that snake scales possess potent visual features. My previous study demonstrated that removing snake scales through digital image processing reduces attention directed toward snakes. Here, I conducted a visual search task using luminance- and contrast-adjusted photographs of snakes and salamanders in monkeys that had never seen these real reptiles and amphibians. This study demonstrates that the presence or absence of snake scales is responsible for the rapid detection of target animals. The monkeys quickly detected one snake photograph from the eight salamander photographs than vice versa. However, when the same salamanders were clothed with snake scales using image processing, the difference in detection speed between snakes and salamanders disappeared. These results are consistent with the snake-detection theory that snakes were a strong selective pressure favoring modifications in the primate visual system that allow them to detect snakes more quickly or reliably. This strongly suggests that primates’ snake detection depends on the snake-scale shapes, which are both snake-specific and common to all snakes.

Development of a Cone Penetration Testing Apparatus with a Textured Shaft

The anisotropy of shear resistance depending on friction direction can be selectively utilized in geotechnical structures. For instance, deep foundations and soil nailing, which are subject to axial loads, benefit from increased load transfer due to greater shear resistance. In contrast, minimal shear resistance is desirable in applications such as pile driving and soil sampling. Previous studies explored the shear resistance by interface between soil and surface asperities of a plate inspired by the geometry of snake scales. In this study, the interface friction anisotropy based on the load direction of cones with surface asperities is evaluated. First, a laboratory model chamber and a small-scale cone system are developed to quantitatively assess shear resistance under two load directions (penetration ⟶ pull-out). A preliminary test is conducted to analyze the boundary effects for the size of the model chamber and the distance between cones by confirming similar penetration resistance values at four cone penetration points. The interface shear behavior between the cone surface and the surrounding sand is quantitatively analyzed using cones with various asperity geometries under constant vertical stress. The results show that penetration resistance and pull-out resistance are increased with a higher height, shorter length of asperity and shearing direction with a decreasing height of surface asperity.

Effects of surface morphology and chemical composition on friction properties of Xenopeltis hainanensis scales

The scales of Xenopeltis hainanensis, a snake that can crawl in fields, valleys, and other places, can serve as inspiration for the design of scale-like bionic materials. We present a systematic morphological, microstructural, chemical, and mechanical analysis, including elastic modulus, hardness, and wear morphology of the scales to understand the friction basis for achieving the reptile requirements. At the surface level, a comb-like arrangement of microstructures on the ventral scales provides more surface area and reduces pressure. The separation of microstructures, along with the bending and delamination of collagen fibrils could contribute to energy dissipation, which helps prevent catastrophic failure at deeper structural levels. At the cross-sectional level, a greater thickness provides more distribution of stresses over a larger volume, reducing local deformation and increasing the resistance to damage. At the material level, the ventral scales show higher modulus (E = 384.65 ± 19.03 MPa, H = 58.67 ± 6.15 MPa) than other regions of snake scales, which is attributed to the increased thickness of the scales and the higher concentration of sulfur (S). The experimental results, combined with Energy-dispersive X-ray spectroscopy and SEM characterization, provide a complete picture of the fiction properties influenced by surface morphology and chemical composition during scratch extension of the Xenopeltis hainanensis scales.

Effect of Surface Morphology and Internal Structure on the Tribological Behaviors of Snake Scales from Dinodon rufozonatum.

Snakes can move freely on land, in lakes, and in other environments. During movement, the scales are in long-term contact with the external environment, providing protection to the body. In this study, we evaluated the mechanical properties and scratching performance of the ventral and dorsal scales from Dinodon rufozonatum, a generalist species that moves on both land and in streams under wet and dry conditions. The results showed that the elastic modulus and hardness of the dry scales were greater than those of the wet scales. The average scale friction coefficient under wet conditions (0.1588) was 9.3% greater than that under dry conditions (0.1453). The scales exhibit brittle damage in dry environments, while in wet environments, ductile damage is observed. This adaptation mechanism allows the scales to protect the body by dissipating energy and reducing stress concentration, ensuring efficient locomotion and durability in both terrestrial and aquatic environments. Understanding how this biomaterial adapts to environmental changes can inspire the development of bionic materials.

A Soft Crawling Robot with Multi-Modal Locomotion Inspired by the Movement Mechanism of Snake Scales

A Soft Crawling Robot with Multi-Modal Locomotion Inspired by the Movement Mechanism of Snake Scales

Scale Microornamentation of Six Species of Venomous Snakes from China

The scales of six venomous snake species from China were examined for their ultrastructure. Scanning electron microscopy was used to observe the scales, which were peeled off from the middle back of the snakes. The results revealed significant differences in the microdermatoglyphic structure patterns between the two families, Viperidae and Elapidae. Viperidae exhibited a “polygonal” structural unit, while Elapidae displayed arcs, pores, and strips as their basic structure. Additionally, there were notable differences in ultrastructure within each family. In Viperidae, Ovophis makazayazaya had a flat structural unit, Trimeresurus stejnegeri had a mound-like uplift near the center with a flat surrounding, and Protobothrops mucrosquamatus had an overall uplifted structure. In Elapidae, Ophiophagus hannah showed obvious differences in the size and arrangement of arc structures. The intraarc structure of Bungarus multicinctus resembled vertebral bodies, while that of Sinomicrurus peinani was columnar in shape. The ultrastructural characteristics of snake scales generally exhibited essential differences among advanced taxonomic units. However, there were detailed differences among species within the same family or genus. The initial formation of microornamentation features in scales may be related to the snakes’ habitat environment. Species formation through isolation is accompanied by the differentiation of these characteristics, and once species formation occurs, the features remain stable until the next species differentiation. There were instances of similar structures found in species with distant evolutionary relationships. This suggests that the microdermatoglyphic structure has taxonomic significance but may not necessarily have phylogenetic significance.

Stable isotopic composition of Bothrops atrox (common lancehead) in four distinct land covers in eastern Amazonia

AbstractSince consumers reflect the isotopic composition of an assimilated diet, stable isotopes can be a useful tool to address the feeding ecology of tropical snakes. This is the first study reporting carbon and nitrogen stable isotopic composition of Bothrops atrox (Linnaeus, 1758) living in different landscapes located in the lower Amazon river, encompassing four main natural landscapes of the Amazon: old‐growth forests, várzeas (flooded forests), savannas, and pastures. Our null hypothesis is that the δ13C of forest specimens of B.atrox is more negative because forests are dominated by C3 plants, while C4 plants are common in the other landscapes. On the other hand, δ15N of forest specimens should be more positive, since the δ15N of old‐growth forests are higher than plants of savanna, várzea, and pastures. Confirming our hypothesis, the δ13C of B. atrox scales of the Tapajós National Forest was approximate −25‰ to −24‰, increased to approximately −23.5‰ to −23.0‰ in the savanna and pasture, and to −21‰ in the várzea, showing an increased contribution of C4‐derived carbon. Some specimens of B. atrox had δ15N as high as 18‰, which is much higher than the average δ15N of the snake's prey (7‰), confirming the apex position of B. atrox in the Amazon region. The δ15N values of the forest specimens were 5‰ higher than the savanna specimens, and this difference decreased to 3‰ between the forest and the pasture, and the várzea specimens. Finally, there were not large differences between δ15N values of livers and scales in any of the landscapes, suggesting a constant diet through time, and reinforcing the possibility of the use of snake's scale as a less invasive and non‐lethal tissue to analyze.Abstract in Portuguese is available with online material.

Snake-scale stimulated robust biomimetic composite triboelectric layer for energy harvesting and smart health monitoring

Extreme frictional wear of triboelectric layers severely hinders long-term sustainability of triboelectric nanogenerators (TENGs) for efficient ambient energy harvesting. The state-of-the-art solutions necessitate sophisticated structural designs and complex packaging constraints, impeding the feasibility of TENGs for practical applications. Herein, inspired by the structures and compositions of snake scales, a biomimetic composite (BC) film is fabricated as a wear-resistant triboelectric contact layer. The successful formation of disulfide bond-facilitated cross-linkage of cysteine proteins in the keratin-based BC film promotes excellent mechanical resilience and durability, thereby solving the material abrasion challenges associated with solid-solid triboelectric layers. The BC film, with toothed microstructure, exhibits improved charge transfer, low friction, and consistent long-term stable outputs compared to commercial films. Furthermore, a self-powered BC film-based TENG is integrated into a bicycle for biomechanical energy harvesting, lightening warning signals for safer night ride with stable 300 V electrical output for at least 6 h. Moreover, a smart health monitoring system is further proposed by embedding TENG-based sensors into the bicycle saddle, accurately capturing the rider’s pelvic-saddle interactions on varying terrain slopes. By leveraging the unique biomechanical information, machine learning-assisted user identification and terrain slope data classifications are achieved with high accuracies, laying a foundation for futuristic smart healthcare applications. This work provides a strategy to reduce material abrasion by exploiting the unique characteristics of a biomimetic material, facilitating a basis for efficient environmental energy harvesting and smart sensing.

Somitic positional information guides self-organized patterning of snake scales.

Two influential concepts in tissue patterning are Wolpert's positional information and Turing's self-organized reaction-diffusion (RD). The latter establishes the patterning of hair and feathers. Here, our morphological, genetic, and functional-by CRISPR-Cas9-mediated gene disruption-characterization of wild-type versus "scaleless" snakes reveals that the near-perfect hexagonal pattern of snake scales is established through interactions between RD in the skin and somitic positional information. First, we show that ventral scale development is guided by hypaxial somites and, second, that ventral scales and epaxial somites guide the sequential RD patterning of the dorsolateral scales. The RD intrinsic length scale evolved to match somite periodicity, ensuring the alignment of ribs and scales, both of which play a critical role in snake locomotion.

Snake Scale-Inspired Poly(vinylidene fluoride)/Ti3CNTx@Polypyrrole Coatings for Ultrawide-Bandwidth Microwave and Visible Light Absorption.

The accuracy of data collected by optical instruments can be greatly impacted by radar band electromagnetic waves (EM) and scattered visible light. Traditional electromagnetic-wave-absorbing (EMA) materials face challenges in effectively attenuating electromagnetic waves within the visible light spectrum. To address this issue, a structural engineering-based assembly strategy was developed to construct PVDF/Ti3CNTx@PPyNF composites with multiple heterogeneous interfaces, inspired by snake scales. And through the self-doping of N elements and the coating process, the material finally exhibits excellent microwave and visible light absorption properties. This approach generates multiple polarization losses of electromagnetic waves, enabling the material to exhibit excellent electromagnetic wave absorption performance. Specifically, the PVDF/Ti3CNTx@PPyNF composite, containing 5 wt % Ti3CNTx@PPyNFs, demonstrates exceptional microwave absorption performance, with a minimum reflection loss of -65.5 dB and an effective absorption bandwidth of up to 6.95 GHz. Additionally, the composite coating exhibits 97.4% visible light absorption performance, providing a promising solution to the challenges of protecting against complex electromagnetic environments.

Effect of internal damping on locomotion in frictional environments.

The gaits of undulating animals arise from a complex interaction of their central nervous system, muscle, connective tissue, bone, and environment. As a simplifying assumption, many previous studies have often assumed that sufficient internal force is available to produce observed kinematics, thus not focusing on quantifying the interconnection between muscle effort, body shape, and external reaction forces. This interplay, however, is critical to locomotion performance in crawling animals, especially when accompanied by body viscoelasticity. Moreover, in bioinspired robotic applications, the body's internal damping is indeed a parameter that the designer can tune. Still, the effect of internal damping is not well understood. This study explores how internal damping affects the locomotion performance of a crawler with a continuous, viscoelastic, nonlinear beam model. Crawler muscle actuation is modeled as a traveling wave of bending moment propagating posteriorly along the body. Consistent with the friction properties of the scales of snakes and limbless lizards, environmental forces are modeled using anisotropic Coulomb friction. It is found that by varying the crawler body's internal damping, the crawler's performance can be altered, and distinct gaits could be achieved, including changing the net locomotion direction from forward to back. We will discuss this forward and backward control and identify the optimal internal damping for peak crawling speed.

Wear Resistance Improvement of Keeled Structure and Overlapped Distribution of Snake Scales

Wear Resistance Improvement of Keeled Structure and Overlapped Distribution of Snake Scales

The Saliency of Snake Scales and Leopard Rosettes to Infants: Its Relevance to Graphical Patterns Portrayed in Prehistoric Art.

Geometrically arranged spots and crosshatched incised lines are frequently portrayed in prehistoric cave and mobiliary art. Two experiments examined the saliency of snake scales and leopard rosettes to infants that are perceptually analogous to these patterns. Experiment 1 examined the investigative behavior of 23 infants at three daycare facilities. Four plastic jars (15×14.5cm) with snake scales, leopard rosettes, geometric plaid, and plain patterns printed on yellowish-orange paper inside were placed individually on the floor on separate days during playtime. Fourteen 7–15-month-old infants approached each jar hesitantly and poked it before handling it for five times, the criterion selected for statistical analyses of poking frequency. The jars with snake scales and leopard rosettes yielded reliably higher poking frequencies than the geometric plaid and plain jars. The second experiment examined the gaze and grasping behavior of 15 infants (spanning 5months of age) seated on the laps of their mothers in front of a table. For paired comparisons, the experimenter pushed two of four upright plastic cylinders (13.5×5.5cm) with virtually the same colored patterns simultaneously toward each infant for 6s. Video recordings indicated that infants gazed significantly longer at the cylinders with snake scales and leopard rosettes than the geometric plaid and plain cylinders prior to grasping them. Logistic regression of gaze duration predicting cylinder choice for grasping indicated that seven of 24 paired comparisons were not significant, all of which involved choices of cylinders with snake scales and leopard rosettes that diverted attention before reaching. Evidence that these biological patterns are salient to infants during an early period of brain development might characterize the integration of subcortical and neocortical visual processes known to be involved in snake recognition. In older individuals, memorable encounters with snakes and leopards coupled with the saliency of snake scales and leopard rosettes possibly biased artistic renditions of similar patterns during prehistoric times.

Immunolocalization of the EDWM-Protein Indicates a Matrix Role in Cornification of Lizard Epidermis

During epidermal differentiation in the scales of lizards and snakes, from the basal layer beta- and later alpha-keratinocytes are generated to form beta-and alpha-corneous layers. In the lizard Anolis carolinensis, minor proteins derived from the EDC (Epidermal Differentiation Complex) are added to the main constituent proteins, IFKs (Intermediate Filament Keratins) and CBPs (Corneous Beta Proteins, formerly indicated as beta keratins). One of these proteins that previous studies showed to be exclusively expressed in the skin, EDWM (EDC protein containing high GSRC amino acids) is rich in cysteine and arginine, amino acids that form numerous –S–S– and electro-static chemical bonds in the corneous material. Light and electron microscopy immunolbeling for EDWM show a diffuse localization in differentiating beta-cells and in some alpha-cells, in particular those of the clear-layer, involved in epidermal shedding. The study suggests that EDWM may function as a matrix protein that binds to IFKs and CBPs, contributing to the formation of the specific corneous material present in beta- and alpha-corneous layers. In particular, its higher immunolocalization in the maturing clear layer indicates that this protein is important for its differentiation and epidermal shedding in A. carolinensis and likely also in other lepidosaurian reptiles.

Cell adhesion and junctional proteins in the developing skin of snakes indicate they coordinate the differentiation of the epidermis.

The development of scales and the sequence of epidermal layers during snake embryogenesis has been studied by immunofluorescence for the localization of cell adhesion, adherens, and communicating cell junctional proteins. At about 2nd/3rd of embryonic development in snakes the epidermis forms symmetric bumps at the beginning of scale formation, and they rapidly become asymmetric and elongate forming outer and inner surfaces of the very overlapped scales seen at hatching. The dermis separates a superficial loose from a deeper dense part; the latter is joined to segmental muscles and nerves, likely acting on scale orientation during snake movements. N-cam is present in the differentiating epidermis and mesenchyme of forming scales while L-cam is only/mainly detected in the periderm and epidermis. Mesenchymal N-cam is associated with the epidermis of the elongating dorsal scale surface and with the beta-differentiation that occurs in the overlapping outer surface of scales. Beta-catenin and Connexin-43 show a similar distribution, and they are mainly present in the periderm and differentiating suprabasal keratinocytes likely forming an intense connectivity during epidermal differentiation. Beta-catenin also shows nuclear localization in differentiating cells of the shedding and beta-layers at late stages of scale morphogenesis, before hatching. The study suggests that intensification of adhesion and gap junctions allows synchronization of the differentiation of suprabasal cells to produce the ordered sequence of epidermal layers of snake scales, starting from the shedding complex and the beta-layer.

Assessment of Sleep-Related Problems in Children with Cerebral Palsy Using the SNAKE Sleep Questionnaire.

Cerebral palsy (CP) represents the most common motor impairment in childhood. The presence of sleep problems has not yet been investigated with an instrument specifically designed for this population. In this hospital-based, prospective study, N = 100 children (M = 7.9, range: 2–18 years) with CP were included. All patients underwent pediatric neurologists’ screening incorporating instruments (Data Collection Form; Gross Motor Functions Classification System, GMFCS; Bimanual Fine Motor Function, BFMF) recommended by the “Surveillance of Cerebral Palsy in Europe (SCPE)”. Parents completed the “Sleep Questionnaire for Children with Severe Psychomotor Impairment (SNAKE)”. Children’s sleep behavior was increasingly conspicuous, with greater gross motor (SNAKE scales: disturbances remaining asleep, daytime sleepiness) and fine motor (additionally SNAKE scale arousal and breathing problems) functional impairment. Overall, a proportion of children showed sleep behavior outside the SNAKE’s normal range. No relevant sleep differences were identified between different CP subtypes and comorbidities. Applying a population-specific questionnaire, children’s functional impairment seems to be more relevant to their sleep behavior than the CP subtype or CP comorbidities.

Skin Friction Directionality in Monotonically- and Cyclically-Loaded Bio-inspired Piles in Sand

Piles can be subjected to axial loading in opposite directions during their installation and service life. For instance, piles for offshore jacket structures and load testing reaction systems are subjected to compressive loading during installation and tensile or cyclic loading during service life. This creates a design dilemma: while a large skin friction can lead to refusal at shallower depths than required during driving, it also promotes a large pile axial capacity. This paper describes the load-transfer behavior of piles with surfaces inspired by the belly scales of snakes that mobilize a direction-dependent skin friction. The investigation presented herein consists of a series of twelve centrifuge pile load tests on bio-inspired and smooth reference piles in dense and loose deposits of Ottawa F65 sand. Test results indicate that greater skin friction forces are mobilized when the bio-inspired piles are displaced in the cranial direction (i.e. soil moving against asperities) relative to the caudal direction (i.e. soil moving along asperities). This is observed during pushing and driving installation, where greater skin friction forces were mobilized during installation by pushing in the cranial direction and driving in the cranial direction required more blows per meter. Similarly, the skin friction mobilized during pullout tests was between 82% and 198% greater in the cranial direction than in the caudal direction, and the skin friction mobilized during pullout by the bio-inspired pile in the cranial direction was between 560% to 845% greater than that mobilized by the reference untextured pile. During cyclic loading, degradation of the skin friction magnitude and pile secant stiffness was observed in both cranial and caudal directions; however, the mobilized magnitudes were generally greater in the cranial direction. Discussion is provided on the potential benefits that the bio-inspired surface texture could realize on the overall performance of axially-loaded piles.

Snake scales record environmental metal(loid) contamination.

Wetland snakes, as top predators, are becoming globally recognised as bioindicators of wetland contamination. Livers are the traditional test organ for contaminant exposure in organisms, but research is moving towards a preference for non-lethal tissue sampling. Snake scales can be used as an indicator of exposure, as many metals bind to the keratin. We used laser ablation with inductively coupled plasma-atomic emission spectroscopy and mass spectrometry (LA-ICP-MS) to quantify the concentrations of 19 metals and metalloids (collectively referred to 'metals' hereafter) in Western tiger snake (Notechis scutatus occidentalis) scales from four wetlands along an urban gradient, and compared them to concentrations measured in captive tiger snake scales. We conducted repeat measures to determine the concentration accuracy of each metal using LA-ICP-MS. Concentrations in wild Western tiger snake scales were significantly higher than in reference tiger snake scales for most metals analysed, suggesting accumulation from environmental exposure. We compared the scale concentrations to sediment concentrations of sampled wetlands, and found inter-site differences between mean concentrations of metals in scales parallel patterns recorded from sediment. Four metals (Mn, As, Se, Sb) had strong positive correlations with liver tissue contents suggesting scale concentrations can be used to infer internal concentrations. By screening for a larger suite of metals than we could using traditional digestive methods, we identified additional metals (Ti, V, Sr, Cs, Tl, Th, U) that may be accumulating to levels of concern in tiger snakes in Perth, Western Australia. This research has progressed the use of LA-ICP-MS for quantifying a suite of metals available in snake scales, and highlights the significance of using wetland snake scales as a non-lethal indicator of environmental contamination.

Investigation of the penetration characteristics of snake skin-inspired pile using DEM

Snake can perfectly utilize its scales to move. Inspired by the snake scales, this study investigated the characteristics of the snake skin-inspired pile penetration process. Six kinds of pile shaft inspired by snakeskin and one normal pile as reference were designed. In order to investigate the influence of scale layouts on the pile and particle responses, the discrete element method was adopted to simulate the process of pile penetration numerically. Effects of three potentially relevant factors, including the scale height, scale length and penetration direction were analyzed. According to the computed results, the snake skin-inspired pile has a greater shaft resistance, causes obvious soil disturbance with aspect to soil displacement and particle rotation compared with the reference pile. The shaft resistance and soil disturbance are positively correlated with the scale height, and inversely relevant with the scale length. The inspired pile installed in cranial direction causes a wider disturbance range in surrounding soil compared with the caudal installation. The cranial penetrated pile contributes to significant soil dilatancy, leading to greater loading–unloading behavior and less major principal stress rotation of surrounding soil.

Indigenous management of Dystocia in ruminant livestock of northern guinea savanna of Nigeria

This study, conducted in Bauchi and Zaria areas of the northern guinea savanna zone of Nigeria involved livestock that encountered problems during parturition Ninety eight Hausa-Fulani livestock holdings involving cattle, sheep and goats were investigated. The study focused on causes of and remedies for dystocia. The study showed that farmers have traditional means of handling difficult birth in ruminants. The identifiable causes of dystocia included age and size of dam, abnormal foetal presentation, poor health, multiple foetuses and weight and sex of the young. The management of this problem included the use of hand to correct foetal position, lubrication of the vulvo-vagina passage with mashed okra fruit for easy passage, and oral administration of solutions from native herbs, snake scales, potash and ashes.