Smoke Exposure System Research Articles

Simultaneous mainstream-sidestream smoke exposure systems I. Equipment and procedures

Equipment and procedures for the simultaneous exposure of cell cultures and rodents to mainstream and sidestream smoke are described. Whole smoke or the gas phase of smoke can be generated and manipulated under a wide range of conditions. Identical smoke can be used for the exposure of cell cultures, perfused lungs, and for the exposure of experimental animals. The equipment is relatively inexpensive, and can be used by small laboratory for efficient, controlled smoke exposure studies. Total particulate matter data are used to illustrate the flexibility of smoke generation, manipulation, and dose variables which can be obtained with the exposure systems.

Deposition and distribution of the total particulate matter of cigarette smoke in mice using a large-capacity smoke exposure system

A newly developed automatic smoke exposure machine (SEM II) was used to generate [ 14C]dotriacontane-labeled University of Kentucky reference 2A1 or 2R1 cigarette smoke. The SEM II is a large-capacity (480 mice) dynamic smoke exposure system in which smoke is routed through the animal containment system as a continuously flowing stream. Mice are restrained about the neck in stock-like holders for “nose-only” exposure. Using standard smoke exposure conditions, the deposition and internal distribution of the total particulate matter (TPM) from cigarette smoke was determined in BC3Fl/Cum male and female mice. Results show: (a) smoke exposure conditions can be varied so that deposition from 30 to 200 μg TPM/lung can be obtained, (b) 80–90% of the TPM deposition was found in the respiratory tissues, (c) the mouse-to-mouse variation for TPM deposition in pulmonary tissue was ∼20%, (d) similar deposition and distribution of TPM was observed in male and female mice, and (e) deposition and distribution of TPM was not altered in mice exposed to smoke on a daily basis over a 6-month period of time.