Smallholder Pig Production Research Articles

Spatiotemporal Drivers of the African Swine Fever Epidemic in Lao PDR

African swine fever (ASF) is a devastating transboundary disease of swine. Following the first report of ASF in China in August 2018, ASF spread through South-East Asian nations in 2019. Without control and containment measures, ASF can decimate smallholder pig holdings and disrupt value chains. This study aimed to describe the spatiotemporal spread of the 2019 Lao PDR ASF epidemic in domestic pigs, identify environmental (protected areas and forests), production (pig ownership), transportation (roads), and social (poverty and ethnicity) risk factors, and recommend measures that could reduce ASF spread. A retrospective spatiotemporal study was conducted at the village level. Information on the date that ASF was first reported from each case location was collected, and the outcome variable of interest “epidemic day” was created. Risk factor information from different sources was extracted for each case location. The association or correlation between epidemic day and risk factors for the spread of ASF was investigated using Kruskal–Wallis tests and Spearman rank correlation statistics. The epidemic started on June 16 and lasted for 190 days, displaying a right-skewed epidemic curve. The directional distribution was rotated approximately 305°, from Southeast to Northwest Laos. Significant risk factors for ASF associated with epidemic day were location in terms of distance from the closest protected natural area P = 0.020 , pig ownership P = 0.005 , road networks P = 0.003 , and poverty indices P < 0.001 . Cases were reported earlier in this epidemic at locations that were closer to protected natural areas, of higher pig ownership, more connected via the national road network, and which experienced elevated poverty. The spatiotemporal pattern described suggests that ASF was introduced via infected pigs from Vietnam. Based on study findings, recommendations for smallholder pig production in Southeast Asia include improving knowledge of swine value chains to inform disease risk and control, monitoring pig transportation, implementing stricter biosecurity measures on the domestic pig population, and providing biosecurity support and education to smallholder pig farmers in poverty.

Co-infections of respiratory pathogens and gastrointestinal parasites in smallholder pig production systems in Uganda

A cross-sectional study was conducted to identify factors for infections of pigs with key respiratory pathogens: porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PPRSv), Mycoplasma hyopneumoniae (M. hyo), Actinobacillus pleuropneumoniae (App), and gastrointestinal (GI) parasites in Uganda. A structured questionnaire was used to collect data on management practices associated with infections. Ninety (90) farms and 259 pigs were sampled. Sera were screened against 4 pathogens using commercial ELISA tests. The Baerman’s method was used to identify parasite species in faecal samples. Logistic regression was done to identify risk factors for infections. Results showed individual animal seroprevalence of PCV2 was 6.9% (95% CI 3.7–11.1), PRRSv 13.8% (95% CI 8.8–19.6), M. hyo 6.4% (95% CI 3.5–10.5), and App 30.4% (95% CI 24.8–36.5). The prevalence of Ascaris spp. was 12.7% (95% CI 8.6–16.8), Strongyles spp was 16.2% (95% CI 11.7–20.7), and Eimeria spp. was 56.4% (95% CI 50.3–62.4). Pigs infested with Ascaris spp. were more likely to test positive to PCV2, odds ratio (OR) 1.86 (CI 1.31–2.60; p = 0.0002). For M. hyo, infection with Strongyles spp. was a risk factor (OR 12.9, p < 0.001). Pigs that had Strongyles and Ascaris spp. Infections (ORs 3.5 and 3.4, p < 0.001 respectively) were likely to have co-infections. The model showed that use of cement, elevated floor, and limiting contacts with outside pigs were protective while using mud and helminth infestations increased risks of co-infections. This study provided evidence that improved housing and biosecurity are critical in reducing pathogen incidence in herds.

Community engagement strengthens pig disease knowledge and passive surveillance in Timor-Leste.

Smallholder pig production in Timor-Leste is culturally and economically important for most households. However, regular and ongoing disease surveillance and pig husbandry training for farmers are limited. This article describes collaborative social and diagnostic research followed by a pilot community engagement program to improve farmer and technician knowledge, skills, and working relationships. There were three phases: (1) A qualitative study in 2020 to explore the experiences and knowledge of 133 pig farmers, 6 village leaders, and 16 district veterinary technicians on pig diseases and reporting, treatment methods, and access to information or assistance. (2) A pilot community engagement program in 3 villages in 2021 with the diagnostic investigation with samples analyzed from 27 dead pigs, and (3) Evaluation of community engagement and training outcomes. Results of the qualitative study revealed limited reporting of sick or dead pigs by farmers to veterinary technicians due to a lack of trust in the veterinary diagnostic system. Most technicians lacked experience with sampling or post-mortems so diagnostic training was undertaken for the pilot disease investigation. Evaluation results showed improved knowledge, motivation, and confidence of government staff and farmers. The credibility of veterinary technicians improved and gave them more confidence to work with communities. Farmers felt supported because all aspects of pig husbandry were addressed, and they were more willing to report dead or sick pigs. The project indicates that improved passive disease surveillance can be achieved by engaging communities in smallholder pig farming in Timor-Leste. Further research and testing of the approach in other districts and countries is recommended.

Effect of artificial insemination in comparison to natural mating on the reproductive performance and profitability of smallholder pig production system in Indian Himalaya

In fragile ecosystems, smallholder pig production systems provide food and nutritional security to resource-poor communities. Pigs are the main livestock raised by indigenous communities in the Himalayan region of India, but their productivity is low for several reasons. The present study aimed to study the pig herd size and to evaluate the impact of artificial insemination (AI) on profitability and sustainability in the small-holder pig production system. A total of 612 AIs were carried out in 483 sows in the farmer's field along with 114 sows that underwent natural breeding. A comparison was made between the reproductive performance of sows following AI and natural breeding. The profitability and economics of AI and natural breeding were also compared. The mean pig population varied from 4.75 to 6.42 in the study region. The farrowing rate, total born piglets (TBPs), and live born piglets (LBPs) were significantly higher (P&amp;lt; 0.001) in artificially inseminated sows compared to naturally bred sows (9.37 vs. 6.28; 8.93 vs. 5.45). Farrowing rate (P= 0.005), TBP, and LBP were significantly (P&amp;lt; 0.001) higher in sows inseminated by female inseminator as compared to male inseminator (81.26 vs. 71.42%; 9.65 vs. 8.80; 9.21 vs. 8.38). The insemination by uneducated farmers resulted in significantly (P= 0.002) lower farrowing rate, TBP (P&amp;lt; 0.001), LBP (P&amp;lt; 0.001), and AI per farrowing (P= 0.042). The farmers who did AI for the third time and more than three times recorded significantly (P&amp;lt; 0.001) higher farrowing rates, TBP, and LBP. The farrowing rate was significantly (P&amp;lt; 0.001) less in sows that were located more than 30 km away from the semen center (66.66 vs. 82.90%). The net return per sow was significantly higher (P&amp;lt; 0.001) in artificially inseminated sows (US$464.8 vs. US$248.11). AI resulted in an 87.33% increase in net returns per farrowing as compared to natural breeding. In conclusion, AI in smallholder pig production systems has the potential to sustainably improve the profitability as well as the food and nutritional security of resource-poor farmers.

Challenges and opportunities of smallholder pig production systems in Rwanda.

To decipher the dynamics, challenges, and opportunities of smallholder pig farming in Rwanda, a household survey was conducted in ten districts of the country. A total of 900 households selected randomly across four provinces and one city, 12 districts, 28 sectors, and villages were interviewed using a pretested printed questionnaire. Most of the respondents were heads of household: 70.9% for western, 63.5% in eastern, 54.1% in northern, and 52.5% in southern province, and their mean age was 46.7years and had widely varying education levels. Crossbred pig genotypes were the most preferred due to their high growth rate and produce large litters compared to local pigs, as well as their high resistance to disease, and can better utilize low-quality feeds compared to pure exotic pigs. Piglets and sows were the biggest populations on most farms. Production of piglets for sale was the main purpose of rearing pigs in 63.7% of the farms. Natural mating was almost (99.1%) exclusive on all farms, implying that artificial insemination is yet to take root in Rwanda. Weeds were reported to be the main feed resource used particularly in wet season, while cereal grain mashes and concentrates were used by very few farmers due to high costs. Dominant challenges related to limited pig and pork markets, high taxation, limited extension services, and disease outbreak were highlighted. The most common diseases affecting pigs were swine erysipelas and internal parasites. Access to veterinary services by the households was limited especially in northern province (20%) leading to wrong diagnosis. Capacity building and provision of access to quality animal health services as well as setting up of markets were proposed as strategies to improve pig production by smallholders in Rwanda and grab a share of the lucrative pork market in the region.

Pigs as a shortcut to money? Social traps in smallholder pig production in northern Uganda

Recent decades have seen a growing market for pork in Uganda. The government and donors have promoted pig rearing as a potential route out of poverty for poor smallholders. The idea is that upscaling and commercialisation of smallholder pig production can be a successful way out of poverty. Drawing on the concepts of trust and social traps, this article describes how pig production fails as a pathway out of poverty in post-conflict communities in northern Uganda due to tensions created by the focus on individual wealth creation. Results from ethnographic fieldwork reveal that there is a strong moral obligation in the studied communities for individuals who fare better to contribute to the community and share their wealth. Social tensions remaining from the period of conflict are stoked by the focus on individual wealth creation in pig production, resulting in acts of harming, stealing and killing other people's pigs. Locally these acts are said to be caused by “jealousy”, which for many smallholders is a more significant problem than disease in pig production. The findings suggest that poverty reduction measures would be more successful if they focused on distributed approaches aimed at raising the general level of welfare in communities and supporting the collective rather than the individual.

Investigation of animal health and husbandry practices in smallholder pig production systems in Timor-Leste.

Enhanced pig productivity on smallholder farms is recognised as a necessary strategy to enhance financial and food security in Timor-Leste where poverty and malnutrition are abundant. While poor pig health is recognised as a main constraint, information on pig herd health and management have not been thoroughly quantified. This study surveyed 120 pig owners (63 were female) and 352 of their pigs in Bacau and Bobonaro municipalities in 2018 to develop baseline information. Our analysis investigated three management systems among surveyed pig owners: confined management, characterised by permanent penning and/or tethering pigs (33.6%), semi-confined management (39.7%) and free-roaming management (27.7%). Free-roaming management was only observed in non-urban villages. Most inputs were limited across all management types with heavy reliance on cooked household scraps to feed pigs (84.7%), limited use of commercial feeds (5.1%), a lack of routine pen cleaning (73.8%), a lack of vaccination against classical swine fever (72.9%), limited use of para-veterinary services when pigs were sick (71.7%), and low treatment rates for pig skin diseases (10.3%) and intestinal parasites (8.7%). A high use of uncontrolled breeding (79.1%) was identified, accompanied by a limited knowledge of oestrus (20.7%) and gestation length (24.1%). Low output was observed with animals mainly sold when money is needed or when they were old. There was poor health with high piglet mortality rate (22.4-24.4%), moderate rates of current illness (22.4%), common occurrence of mites (12.2%), and high faecal presence of A. suum (29.0%), T. suis (10.2%), and S. ransomi (22.7%). To overcome the widespread constraints to productivity affecting all management systems, and to limit the impacts of highly infectious and often fatal African swine fever which was first reported in Timor-Leste pigs in September 2019, improved animal health and veterinary support, and education on pig management and suitable available nutrition sources are needed.

Carbon emissions from smallholder pig production in China: a precise account based on farmers' survey.

Carbon emission from pig production is an issue of great importance owing to its effect on global warming. Differed from widespread large-scale pig farms in North America and Europe, small-scale and smallholder pig farms are mainly concentrated in China. However, information on carbon emissions from Chinese smallholder pig farms is limited. Additionally, large amounts of drugs and vaccines have been applied during smallholder pig production in China, yet their contribution to carbon emissions is unclear. Therefore, detailed dataset which records all inputs during a pig's entire life cycle should be obtained, so as to accurately determine the magnitude of carbon emissions from Chinese smallholder pig farms. This study took Yancheng, eastern China, as an example and adopted the carbon footprint (CF), life cycle inventory (LCI), and Intergovernmental Panel on Climate Change (IPCC) greenhouse gas (GHG) field calculations to accurately estimate the GHG emissions resulting from pig production of China. Furthermore, the contributions of vaccine application and other driving forces behind GHG emissions were identified using statistic methods. In the study area, the pig CFs in the nursery period, fattening period, and full life cycle were 5.83, 4.73, and 6.75kg CO2 eq·kg-1 respectively. The CF of pig production in the study area varied from 4.74 to 9.48kg CO2 eq·kg-1, with an average of 6.75kg CO2 eq·kg-1; this average was, overall, higher than that of large-scale pig farms in North America and Europe. GHG emissions from manure (42.87%) and fodder (27.77%) were responsible for a large proportion of the total CF. Normal vaccine inputs contributed highly (15.33%) to the total CF. The contribution of vaccine application to the CF is roughly evaluated, suggesting it may be a potentially important source of GHG emissions in pig production and should receive more attention in the future. Furthermore, GHG emissions from smallholder pig production farms can be significantly reduced by developing a mixed crop-livestock system, increasing the application of organic fertilizers, and installing biogas digesters.

A Mix of Old British and Modern European Breeds: Genomic Prediction of Breed Composition of Smallholder Pigs in Uganda.

Pig herds in Africa comprise genotypes ranging from local ecotypes to commercial breeds. Many animals are composites of these two types and the best levels of crossbreeding for particular production systems are largely unknown. These pigs are managed without structured breeding programs and inbreeding is potentially limiting. The objective of this study was to quantify ancestry contributions and inbreeding levels in a population of smallholder pigs in Uganda. The study was set in the districts of Hoima and Kamuli in Uganda and involved 422 pigs. Pig hair samples were taken from adult and growing pigs in the framework of a longitudinal study investigating productivity and profitability of smallholder pig production. The samples were genotyped using the porcine GeneSeek Genomic Profiler (GGP) 50K SNP Chip. The SNP data was analyzed to infer breed ancestry and autozygosity of the Uganda pigs. The results showed that exotic breeds (modern European and old British) contributed an average of 22.8% with a range of 2–50% while “local” blood contributed 69.2% (36.9–95.2%) to the ancestry of the pigs. Runs of homozygosity (ROH) greater than 2 megabase (Mb) quantified the average genomic inbreeding coefficient of the pigs as 0.043. The scarcity of long ROH indicated low recent inbreeding. We conclude that the genomic background of the pig population in the study is a mix of old British and modern pig ancestries. Best levels of admixture for smallholder pigs are yet to be determined, by linking genotypes and phenotypic records.

Characterization of smallholder pig production systems in the Western Cape, South Africa.

This study set out to characterize the farming and management practices of smallholder pig production systems in the Western Cape, South Africa. Data was gathered in three municipalities: Khayelitsha (27 farmers), Mamre (26 farmers) and Malmesbury (22 farmers) with the use of a structured questionnaire through both face-to-face and telephonic interviews. The study revealed that smallholder pig farmers in the Western Cape owned on average 37.4 ± 50.5 pigs. Majority of farmers (61%) made use of tattoo markings to identify their pigs, 6% made use of ear tags and 4% clipped the ears of their pigs. More than 25% of farmers did not use any method of identification to mark their pigs. More than half of the respondents (51%) do not have formal recordkeeping systems in place. Livestock theft was found to be a major problem amongst farmers. No differences were found amongst the three study areas and the type of housing system used (P > 0.05). The most popular materials pens were constructed of across the three areas were wood (40%), corrugated iron (16%) or a combination of the two (32%); other materials used were cement blocks (5%), polystyrene (1%) or plastic (1%). Pig farmers kept 43% Large White, 43 % crossbred and 14% Landrace across the three districts. Commercial feed was used by 61% of Mamre farmers whereas Khayelitsha farmers fed an equal amount of by-products (44%) and mixed (44%) feed to their pigs, whereas Malmesbury fed 44% mixed, 33% commercial and 24% by-products obtained from factories to their pigs. Piglet mortality due to the sow lying on her piglets was experienced by 47% of farmers interviewed, whilst 29% had experienced piglet mortalities due to the cold. Perturbingly, 76% of farmers did not send dead pigs for a post-mortem, stating that they could not rely on veterinarians or afford their services. Smallholder pig farmers in Malmesbury (68%) and Khayelitsha (70%) sold their pigs to the informal market, indicating that keeping pigs provided a source of revenue. Mamre marketed 88% of their pigs as weaners, Khayelitsha marketed 73% of their pigs as growers, whereas Malmesbury kept 32% of their pigs as breeding stock. Khayelitsha and Malmesbury marketed their growers at 63.3 ± 41.2 kg and 51.5 ± 30.2 kg, respectively, to the informal market. The study highlights the farming practices of smallholder pig farmers in three districts in the Western Cape. There a few differences in the practices of these farmers, but overall, there are similar limitations that hinder them from improved production, such as limited recordkeeping, theft, lack of infrastructure and finances for post-mortem examination. Smallholder pig farmers in the Western Cape are still mainly selling to the informal market. This informal market is not fully characterized yet in the Western Cape, and more studies are needed to further determine and investigate the benefits of only selling to the informal market.

Smallholder pig production system of Assam

The study was carried out to attain information on smallholder pig production systems of Assam. Stratified purposive and random sampling design was followed for selection of respondents in two districts namely Tinsukia and Dhemaji located in sub-Himalayan terrain. The socioeconomic parameter of the farmers were delineated. Mostly the pigs were reared in semi-intensive to loosehouse system. Major feed ingredients were kitchen waste and locally available, seasonal unconventional feed. There was prevalence of local breeds namely Ghungroo and Doom pigs along with crossbred animal mostly of Hampshire and Large White Yorkshire crosses. The field level performance was evaluated. The study revealed the major constrains and opportunity for improvement of the sector in the surveyed area.

Pig Shed Wastewater Recycling Model for Smallholder Pig Production System of Northeast Himalayan Region

Pig Shed Wastewater Recycling Model for Smallholder Pig Production System of Northeast Himalayan Region

Success rate, genetic improvement and economic analysis of artificial insemination delivery models for smallholder pig production systems

Context Smallholder pig production systems in the north-eastern region of India are characterised by inefficiency due to use of unselected local pigs, and multiple breeding constraints such as paucity of superior germplasm, high mating costs, and poor access to artificial insemination (AI). Effective AI is the most viable option for genetic improvement and enhancing pig productivity. Aim The study was designed to assess the success rate and economic efficacy of four AI delivery models (Models I–IV): (I) AI at farmer’s doorstep through direct linkage to tribal farmers; (II) AI at farmer’s doorstep through trained, educated youth; (III) oestrus synchronisation and fixed-time AI; and (IV) mobile van-based AI delivery system. The study also sought to ascertain the benefits of adopting a crossbreeding program through AI over the existing use of local pigs for smallholder systems. Methods Semen collected from nine Hampshire boars was routinely utilised for AI after processing as per standard protocols. For Models I–IV, respectively, 259, 154, 183 and 284 sows from 75 villages/clusters were inseminated. Reproductive and litter performance as well as insemination cost were evaluated for each model. Growth performance of crossbred pigs obtained through AI was compared with that of local pigs in the smallholder production system. Key results Farrowing rate and mean litter size at birth were 74.90% and 8.57 ± 0.52 for Model I, 68.83% and 8.08 ± 0.37 for Model II, 80.87% and 9.31 ± 0.41 for Model III, 75.00% and 8.54 ± 0.64 for Model IV, and 81.58% and 6.81 ± 0.29 for natural service. Farrowing rate, litter size at birth and at weaning, and litter weight at weaning were significantly (P &lt; 0.05) affected by AI delivery model in the order: Model III &gt; Model I = Model IV &gt; Model II. Litter weight at birth was significantly (P &lt; 0.05) affected by AI delivery model in the order: Model III &gt; Model I = Model II = Model IV. Lowest cost per insemination was found for Model IV (INR 319.00) followed by Model II (INR 449.00), Model III (INR 629) and Model I (INR 899). All models had lower cost than the natural service system (INR 2200). The growth performance of crossbred pigs obtained through AI was significantly (P &lt; 0.01) higher than of the local pigs reared in the same production system. Conclusion The present study justifies the accessibility, feasibility and potential benefit of AI delivery models in smallholder/backyard pig production systems in the north-eastern region of India. Use of these AI delivery models will overcome the breeding constraints and reward farmers with more rapid genetic improvement in productivity with lower production costs. Model III (oestrus synchronisation and fixed time AI) shows best productivity and Model IV (mobile van-based AI delivery system) has lowest cost. Implications These AI delivery models will be effective for sustainable and economic piggery development and improve the socioeconomic livelihood of pig farmers in South Asian countries.

Artificial Insemination Training Program for Smallholder Pig Farms in Gauteng Province, South Africa

The aim of this study was to facilitate artificial insemination training to enhance sustainable pig production within the developing smallholder pig production sector in Gauteng Province, South Africa. Eighteen smallholder pig farmers with requisite structures (pig house, pens), pigs (large white, landrace duroc or South African indigenous) and management (feeding, cleaning and record keeping) capacity were trained on routine pig management and artificial insemination procedures in a &amp;ldquo;learning by doing&amp;rdquo; on-farm supervised programme administered by Agricultural Research Council, Animal Production pig training team. Following estrus detection, 96 sows were artificially inseminated and 31 naturally served (NS). Farrowing rates (FR), total born (TB) and born alive (BA) piglets were recorded. The occurrence ccurrence of mummified fetuses (0.019 vs. 0.022%) and weak piglets (0.038 vs. 0.049%) did not differ between artificially inseminated sows and naturally mated sows. Born alive, birth weight and weaning weight were higher for artificial inseminated sows. The average litter size was 15&amp;plusmn; and 13&amp;plusmn;, birth weight 1.98&amp;plusmn;0.79 kg and 1.48&amp;plusmn;0.58 kg and weaning weight 9.89&amp;plusmn;0.87 kg and 7.23&amp;plusmn;0.71 kg for the AI and NS litters, respectively. Farmer demographic factors (age, gender and educational level) had no effect on farrowing rate, total born and piglets born alive. Therefore, implementation of artificial insemination techniques and pig production training was feasible under a smallholder pig production system.

Combining conventional and participatory approaches to identify and prioritise management and health-related constraints to smallholder pig production in San Simon, Pampanga, Philippines

Pork is the main meat produced and consumed in the Philippines. The majority of pigs are raised by smallholders who experience a range of constraints to their pig production. This study presents the findings of the first part of an overarching project that used an Ecohealth approach and aimed to improve the production and competitiveness of the smallholder pig system in an area of the Philippines. A participatory approach was embraced, combining conventional and participatory epidemiology methods followed by a stakeholder discussion. The first aim was to identify management and health-related constraints to pig production among smallholder famers in San Simon, Pampanga, Philippines. The second aim was for the project team and stakeholders to jointly prioritise activities for the immediate future to address these constraints. Key management and health-related constraints identified included inadequate water supply to pigs, particularly lactating and gestating sows, and a range of feeding-related issues. Diarrhoea was recognised as the disease syndrome of highest priority and limited record keeping meant that farmers were unable to assess the productivity and profitability of their pig farming enterprises. Actions jointly prioritised by stakeholders and the project team were: the appointment of a project coordinator within each barangay; conduct two sets of seminars, the first covering water and nutrition and the second piglet management and diarrhoea, to be delivered by technical experts but with farmer “trusted sources” also sharing their experiences; development of easily understandable leaflets and posters covering key technical information; promotion of nipple drinkers attached to five-gallon water containers and creep boxes for piglets, and conduct of a record keeping workshop with a small group of innovative farmers to develop a useful and usable tool for record keeping. The use of multiple approaches to data-gathering enabled triangulation of study findings. Without any one of these components the understanding of the pig production system would have been less complete and it is possible that the proposed actions would not have been as well-tailored to the needs of the farmers. The participatory approach, in particular the stakeholder discussion, provided the opportunity to embrace the “deciding together” and “acting together” stances of participation rather than the lower “information giving” stance, thereby giving stakeholders greater ownership of the future activities of the overarching project and beyond.

Evidence for the presence of African swine fever virus in apparently healthy pigs in South-Kivu Province of the Democratic Republic of Congo

African swine fever (ASF) is the most important disease constraining smallholder pig production in the Democratic Republic of Congo, causing high mortality in domestic pigs with severe impacts on the livelihoods of local populations. This study was conducted with the aim of determining the prevalence of ASF and circulating virus genotypes in asymptomatic pigs raised on smallholder farms in the South Kivu province to understand the transmission dynamics of ASF and ultimately improving disease control. A cross-sectional survey was carried out in 5 districts where 267 pig blood were screened for both antibody and viral genome using indirect Enzyme Linked Immunosorbent Assay (ELISA) and polymerase chain reaction (PCR) respectively. Additionally, amplicons from PCR positive samples were sequenced by Sanger method for genetic analysis of ASF virus (ASFV) based on the C-terminal region of the B646L gene that encodes the major capsid protein p72 and the gene E183L encoding the p54 protein. The overall seroprevalence obtained based on antibody to p30 protein was 37 % and was significantly higher (P < 0.05) in adult (>1 year) animals (44.7 %) than in younger (<1 year) ones (33.5 %). Moreover, the seropositivity varied significantly (P < 0.05) according to the pig husbandry system practiced within the districts investigated with Uvira (55 %) and Mwenga (42.2 %) having the highest ASFV antibodies, while the lowest (10.5 %) were in Kalehe. Free-range pigs exhibited a higher level of seropositivity to ASFV antibody (68.9 %) than pigs kept in the pigsty housing system (21.6 %). However, no statistically significant differences (P > 0.05) were observed when sex of the animal and breed were factored. PCR detection of ASFV amplified a specific band of expected size (257 bp) in 61 out of 267 blood samples, confirming the presence of the viral DNA in 22.8 % of asymptomatic domestic pigs. Statistical analysis revealed that ASFV infection in domestic pigs varied significantly (p < 0.001) according to geographical location and breed, with the highest infection rate found in Walungu district (33.7 %) while the lowest was registered in Kalehe (15.8 %). Local pigs (27.2 %) were more infected than crosses (9.2 %).Phylogenetic analyses based on partial sequences of the p72 and p54 genes revealed that all the ASFV detected belonged to genotype IX, which has previously been reported in other parts of DR Congo, and was clustered together with isolates from Kenya, Uganda and Republic of Congo. This study avails the first evidence of the presence of ASF virus in domestic pigs in the absence of outbreaks in South Kivu province, eastern DR Congo, indicating a need to raise awareness among pig farmers and veterinary authorities on the application of biosecurity measures and good husbandry practices to control the disease.

Slaughterline records of various postmortem pathological lesions and their influence on carcass and meat quality in slaughtered pigs.

The aim of this study was to identify the prevalence of pathological lesions in pigs from small-scale farms and to determine associations between pathological lesions and hematological parameters, and carcass and meat quality in slaughtered pigs. The study was conducted on 625 pigs (~115kg) originating from 20 small-scale farms. Any signs of pneumonia, pleurisy, pericarditis, and liver milk spots were recorded as present or absent. Complete blood count was investigated. The following carcass quality parameters were measured: live, hot and cold carcass weights, cooling loss, dressing percentage, backfat thickness, and meatiness. Meat pH and temperature were measured 45min postmortem. Of the 625 examined pigs, 41.8% had pneumonia, 23.5% pleurisy, 2.7% pericarditis, and 29.9% liver milk spots. The presence of pathological lesions in slaughtered pigs adversely affected hematological parameters, reduced live, hot and cold carcass weights, and meatiness and had deleterious effects on meat quality (higher pH45min and higher prevalence of dark, firm and dry meat). In conclusion, this study showed a high prevalence of pathological lesions in slaughtered pigs, indicating serious health problems in smallholder pig production systems. The presence of single and, especially, multiple pathological lesions in slaughtered pigs negatively affected hematological parameters, and carcass and meat quality.

Ineffectiveness of meat inspection in the detection of Taenia solium cysticerci in pigs slaughtered at two abattoirs in the Eastern Cape Province of South Africa.

Porcine and human cysticercosis, caused by the larval stage of tapeworm Taenia solium, is a zoonosis in southern Africa and known to be endemic in South Africa, mainly in Eastern Cape Province. No efforts to control or eradicate this parasite have been made, despite the increasing occurrence in most Eastern Cape districts, except for routine meat inspection at local abattoirs. The parasite poses a potentially serious agricultural problem, public health risk and economic loss amongst Eastern Cape smallholder pig production communities. The objective of this study was to determine the effectiveness of routine meat inspection for the detection of porcine cysticercosis in pigs from rural smallholder/subsistence production systems in Eastern Cape Province villages. The effectiveness of meat inspection, by registered meat inspectors, in the detection of pigs infected with T. solium cysts was assessed and compared with whole carcass dissection as the "gold standard" method. The commercial antigen enzyme linked immunosorbent assay (B158/B160 Ag-ELISA) kit screened all the slaughtered animals. The proportion of pigs found infected with T. solium cysts, as measured by meat inspection, was lower (5%, 9/180) than with carcass dissection (18.9%, 34/180) and B158/B60 Ag-ELISA test (21.6%, 38/176). Four out of 180 carcasses were heavily infested with T. solium cysts, evenly distributed throughout the carcasses, to a level impossible to enumerate. Of the remaining 176 carcasses, approximately 526 cysticerci, distributed at various anatomical regions of the pig, were counted during carcass dissection. Sites with higher cyst counts, such as the back and hind leg, do not form part of the normal meat inspection regime. The level of agreement (Kappa statistic) between dissection (gold standard) and meat inspection of the two districts was negative (-0.1955). There was a slight agreement in the Kappa statistic (0.0328) between dissection and B158/B60 Ag-ELISA. This study confirms that current meat inspection procedures alone are not sufficiently sensitive to detect all cases of porcine cysticercosis at the abattoirs and require modifications, or should be supplemented by other methods. A risk-based meat safety assurance system, such as HACCP, that considers specific food safety aspects before and after the abattoir (point of slaughter) should be followed. Before slaughter, aspects such as origin, husbandry practices and on-farm animal health control should be considered; after slaughter, the abattoir should inform the next entity in the supply chain of the limitations of meat inspections and the real meaning of an "Approval" stamp. New validated testing methods that can be routinely used should be developed, and government should develop policies and legislation that promotes a risk-based meat safety assurance system throughout the food supply chain.

The importance of on-farm biosecurity: Sero-prevalence and risk factors of bacterial and viral pathogens in smallholder pig systems in Uganda

BackgroundThe productivity of pigs in smallholder systems is affected by high disease burden, most of which might not be obvious, with their epidemiology and impact being poorly understood. This study estimated the seroprevalence and identified the risk factors of a range of bacterial and viral pathogens of potential economic and public health importance in domestic pigs in Uganda. A total of 522 clinically healthy pigs were randomly selected from 276 pig farms in Masaka (142) and Lira (134) districts of Uganda in 2015. ResultsOverall the highest animal prevalence was found for Streptococcus suis 73.0% (CI95: 67.0–78.3) in Lira and 68.2% (CI95: 62.7–73.4) in Masaka; followed by Porcine circovirus type 2 with 50.8% (CI95: 44.5–57.2) in Lira and 40.7% (CI95: 35.2–46.5) in Masaka and Actinobacillus pleuro-pneumoniae, 25.6% (CI95: 20.4–31.6) in Lira and 20.5% (CI95: 16.2–25.6) in Masaka. Mycoplasma hyopneumonia prevalence was 20.9% (CI95: 16.2–26.6) in Lira and 10.1% (CI95: 7.1–14.1) in Masaka, while Porcine parvovirus was 6.2% (CI95: 4.0–9.7) in Masaka and 3.4% (CI95: 1.7–6.6) in Lira. Less common pathogens were Influenza A, 8.5% (CI95: 5.6–12.8) in Lira and 2.0% (CI95: 0.9–4.5) in Masaka and Porcine Reproductive and Respiratory Syndrome Virus, 1.7% (CI95: 0.7–4.3) in Lira and 1.3% (CI95: 0.5–3.5) in Masaka. Even less common was Rotavirus A with 0.8% (CI95: 0.2–3.0) in Lira and 0.7% (CI95: 0.2–2.5) in Masaka; the same was for Aujeszky virus with 0.4% (CI95: 0.7–2.4) in Lira and 0.0% (CI95: 0.0-0.1) in Masaka. Co-infection with two pathogens was common and there was a significant association of M. hyo and PCV2 co-occurrence (p = 0.016). Multivariate analysis showed that for S. suis the use of disinfectant reduced odds of sero-positivitey (OR = 0.15; p = 0.017) and pigs less than 6 months were more likely to be infected than older pigs (OR = 3.35; p = 0.047). For M. hyo, crossbred pigs had higher odd of infection compared to local breeds (OR = 1.59; p < 0.001). ConclusionsThe studied pathogens have high prevalences in smallholder pig production systems and might be silent killers, thus affecting productivity and there is a possibility that some pathogens could spread to humans. Given the limited knowledge of veterinary workers and the poor diagnostic capacities and capabilities in these systems, the diseases are potentially usually under-diagnosed. These findings constitute baseline data to measure the impact of future interventions aiming to reduce disease burden in the pig production systems in Uganda.

Oestrus synchronization with fixed-time artificial insemination in smallholder pig production systems in north-east India: Success rate and benefits

Pig production is an important component of the farming systems that are practised in the north-eastern region of India and it has special significance for improving the socio-economic status of tribal farmers in the region. In an effort to optimize the production efficiency of rural pig production systems, the potential influence of oestrus synchronization and fixed-time artificial insemination (AI) on synchronizing husbandry programmes was investigated. A total of 117 crossbred sows, selected from various village production units, were randomly allocated to two (control and treatment) groups. The sows in the treatment group (n = 81) received 800 IU of equine chorionic gonadotropin (eCG), followed by 500 IU of human chorionic gonadotropin (hCG) 72 hours after the eCG injection. The sows in the control group (n = 36) served as the untreated control. The onset of oestrus, oestrus signs, oestrus intensity, and duration of oestrus were observed after treatment. The oestrus sows were inseminated twice at 24 h and 36 h, respectively, after hCG injection at various village production units. Results revealed that 86.4% of sows in the treatment group exhibited all the behavioural and physical signs of oestrus. The average interval between treatment and onset of oestrus was 84.8 ± 2.43 hours. The mean oestrus intensity of sows in the treatment group was 2.3 (when assessed on a scale of 1 to 3), which did not differ significantly from the oestrus intensity exhibited by the control group. The duration of oestrus varied from 36 to 56 hours in the treated sows, which was significantly longer when compared with the duration of oestrus in the control group. The study obtained a farrowing rate of 82.6% and litter size of 9.2 ± 0.32 after oestrus synchronization with timed insemination. Oestrus synchronization facilitated the insemination of a batch of sows at particular locations covering two to three village clusters, which drastically reduced the transport costs for the semen. Oestrus synchronization facilitated the effective implementation of AI by coordinating the insemination of batches of sows, thus reducing shipping and insemination costs. Improving heat detection contributed to shortening the weaning to oestrous interval in smallholder pig production system, which in turn decreased production costs. ______________________________________________________________________________________ Keywords : Assisted reproduction, farrrowing rate, insemination cost, reproduction efficiency