Small Region Research Articles

Evaluation of Tidal Asymmetry and Its Effect on Tidal Energy Resources in the Great Island Region of the Gulf of California

Hydrokinetic tidal energy is one of the few marine renewable energy resources with sufficiently mature technology for commercial exploitation. However, several parameters affect its exploitability, such as the minimum speed threshold, ambient turbulence levels, or tidal asymmetry, to name but a few. These parameters are particularly important in regions with lower mean speeds than those in first-generation sites, such as the North Sea. The Gulf of California is one of those regions. In this paper, a Delft3D Flexible Mesh Suite (Delft3D FM) model in barotropic configuration is set up over the Gulf of California using a flexible mesh with resolution varying from O (500 m) in the deep regions to O (10 m) in the coastal regions. A simulation is run over the year of 2020, with a tidal forcing of 75 components. The model is validated at four tidal gauge locations and four Acoustic Doppler Current profiler (ADCP) locations. The speed, U, and tidal power density (TPD) indicators used for the validation were the annual means, the annual means for speeds above the 0.5 m s−1 threshold, the annual means of the spring tide maxima, and the annual maxima. The contour maps of the annual means, that is, the annual means for speeds above the 0.5 m s−1 threshold, allow us to identify tidal energy hot spots throughout the Gulf of California, particularly in the Great Island region (GIR). In this region, these hot spots have higher U and TPD values, in agreement with previous studies. The patterns of circulation around Tiburón Island and San Esteban Island on the East, and Ángel de la Guarda Island and San Lorenzo Island on the West, the four islands in the region with the highest tidal energy potential, are also discussed while recognizing that Tiburón Channel, between Tiburón Island and San Esteban Island, has proved to be the best siting location, based on the technical results obtained so far. The hot spots sites are further characterized by computing the tidal asymmetry in these small regions, showing the locations of the sites with smallest asymmetry, which would be the best for tidal energy exploitation. The hot spots around San Esteban Island are particularly important because they have the largest TPD in the GIR, with the model predicting a TPD on the order of 500–1000 W m−2. Here, complementary field measurements obtained with two ADCPs, close to San Esteban Island, one at 15 m depth, SEs (shallow region), and the other at 60 m depth, SEd (deep region), produced TPDs of 1200 W m−2 and 400 W m−2, respectively. The analysis of the vertical profiles and the tidal asymmetry over the vertical shows the importance of developing 3D models in future investigations.

Characterization of the complete chloroplast genome sequence of Schisandra propinqua subsp. sinensis (Oliv.) R.M.K.Saunders 1997 (Schisandraceae)

Schisandra propinqua subsp. sinensis (Oliv.) R.M.K. Saunders 1997, a woody vine plant esteemed for its medicinal properties, has garnered attention in botanical research. In this study, we elucidated the complete chloroplast genome sequence of S. propinqua. The genome spans 145,562 bp and comprises a large single-copy (LSC) region of 94,164 bp, a small single-copy region of 18,294 bp (SSC), and a pair of inverted repeats (IR) of 16,552 bp. Notably, S. propinqua exhibits an overall GC content of 36.2%. Annotation revealed a total of 116 genes, encompassing 81 protein-coding genes, 27 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. Phylogenetic analysis unveiled a close relationship among Schisandraceae, indicating the evolutionary proximity. This comprehensive genomic dataset provides valuable insights into the genetic makeup and evolutionary dynamics within the Schisandra genus.

The complete chloroplast genome sequence of Primula medogensis (Primulaceae) and its phylogeny

Primula medogensis W.B Ju, B. Xu & X.F. Gao 2023, a new species categorized under P. sect. Cordifoliae, was officially described in 2023. Given its recent classification, the genetic resources for this species are currently very limited. Here, we sequenced and assembled the first complete chloroplast genome of P. medogensis using Illumina sequencing technology. The complete chloroplast genome of P. medogensis is 151,486 bp in length, exhibiting a typical quadripartite structure. It consists of a large single-copy region (LSC; 83,407 bp) and a small single-copy region (SSC;17675 bp), separated by a pair of inverted repeat regions (IRs; 25202 bp). A total of 131 genes were annotated, including 86 protein-coding, 37 tRNA, and eight rRNA genes. The overall GC content was 37.1%. Phylogenetic analysis of 59 Primula species revealed a close relationship between P. medogensis and P. calliantha subsp. bryophila.

The complete chloroplast genome sequence and phylogenetic analysis of Hordeum pusillum Nutt., 1818 (Poaceae)

Hordeum pusillum (Hordeum pusillum Nutt., 1818) is an annual barley that is native to the west of the North America and widespread in southern United States and tropical America. In this study, we have provided the first complete characterization of the chloroplast genome of H. pusillum. Our research revealed that the circular chloroplast genome of H. pusillum consists of a large single-copy region (LSC: 80,853 bp), a small single-copy region (SSC: 12,745 bp), and a pair of inverted repeat regions (IRs: 21,587 bp), totaling 136,772 bp in length. Within the chloroplast genome of H. pusillum, 91 protein-coding genes, 38 tRNA genes, and ten rRNA genes were identified. To determine the evolutionary relationship of Hordeum species with reported chloroplast genome sequences, we constructed a phylogenetic tree using the entire chloroplast genome sequences. The evolutionary position of H. pusillum corresponds to its geographical location. The chloroplast genome of H. pusillum provided in this study may have significant implications for the phylogenetic study of Poaceae species.

The complete chloroplast genome of Camellia huulungensis Rosmann et Ninh, a golden Camellia species endemic to Vietnam

Camellia huulungensis Rosmann & Ninh 1997, belonging to the sect. Chrysantha, holds important ornamental value and medicinal value. In this study, the complete chloroplast genome sequence of C. huulungensis was assembled using high-throughput sequencing technology. The entire length of chloroplast genome is 156,546 bp and contains a small single-copy region (18,257 bp), a large single-copy region (86,219 bp), and a pair of inverted repeat regions (26,035 bp). A total of 133 genes were annotated, including 88 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content is 37.33%. The phylogenetic analysis showed that C. huulungensis is sister to C. aurea. The results can provide genetic data for further phylogenetic studies of Camellia.

The complete chloroplast genome and phylogenetic analysis of Garcinia esculenta Y. H. Li. 1981 (Clusiaceae)

Garcinia esculenta Y. H. Li. 1981 (Clusiaceae) is an endemic tree species in China, primarily found in western and northwestern Yunnan Province. In this research, the complete chloroplast genome of G. esculenta was sequenced using the Illumina NovaSeq6000 platform. The result showed that the length of the complete chloroplast genome was 155,853 bp, which was composed of a large single-copy region (LSC) of 84,534 bp, a small single-copy region (SSC) of 17,175 bp, and a pair of inverted repeat (IR) regions of 27,072 bp. The overall GC content was 36.1%. The complete chloroplast genome encompassed 128 genes, comprising 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of the complete chloroplast genome sequences of 22 species revealed that G. esculenta is most closely related to G. oblongifolia.

The complete chloroplast genome of Tabebuia rosea (Bignoniaceae)

Tabebuia rosea is a world-renowned woody plant with colorful flowers in full bloom. In addition to its high ornamental value, it also has ecological and medicinal value. In this study, the complete circular chloroplast genome of T. rosea was reconstructed and annotated using Illumina sequencing. The chloroplast genome was 158,919 bp in size with GC content of 38.21%, including a large single-copy region of 85,823 bp, a small single-copy region of 12,816 bp, and a pair of inverted repeats of 30,140 bp. It encoded 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Based on current available chloroplast genome sequences, the phylogenetic analysis indicated that T. rosea was clustered with T. nodosa and H. chrysanthus. This study provided insights into the evolutionary relationships among different species of Bignoniaceae.

The complete chloroplast genome of Fuchsia standishii J. Harrison, 1840 (Onagraceae) from Yunnan, China

Fuchsia standishii J. Harrison, 1840, a perennial shrub, is renowned for its vividly colored and uniquely shaped blooms, which have an extended flowering season. Commonly cultivated as an ornamental potted plant, it is utilized in traditional Chinese medicine. In this study, we successfully sequenced and assembled the complete chloroplast genome of F. standishii using high-throughput Illumina sequencing technology. The assembled chloroplast genome displays a typical quadripartite structure, with a total length of 156,391 bp. It consists of a pair of inverted repeat regions (IRs), each measuring 25,069 bp, separated by a large single-copy region (LSC) of 87,754 bp and a small single-copy region (SSC) of 18,499 bp. The overall GC content of the genome is 37.60%. The genome includes a total of 129 genes, comprising 84 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis of 17 complete chloroplast genomes revealed that F. standishii forms a monophyletic group with the entire Circaea. This study provides a molecular foundation for future phylogenetic research on Fuchsia.

Extraembryonic Membranes and Placentation in the Mexican Snake Conopsis lineata.

Extraembryonic membranes provide protection, oxygen, water, and nutrients to developing embryos, and their study generates information on the origin of the terrestrial egg and the evolution of viviparity. In this research, the morphology of the extraembryonic membranes and the types of placentation in the viviparous snake Conopsis lineata are described through optical microscopy during early and late gestation. When embryos develop inside the uterus, they become surrounded by a thin eggshell membrane. In early gestation, during stages 16 and 18, the embryo is already surrounded by the amnion and the chorion, and in a small region by the chorioallantois, which is product of the contact between the chorion and the growing allantois. A trilaminar omphalopleure covers the yolk sac from the embryonic hemisphere to the level of the equator where the sinus terminalis is located, and from there a bilaminar omphalopleure extends into the abembryonic hemisphere. Thus, according to the relationship of these membranes with the uterine wall, the chorioplacenta, the choriovitelline placenta, and the chorioallantoic placenta are structured at the embryonic pole, while the omphaloplacenta is formed at the abembryonic pole. During late gestation (stages 35, 36, and 37), the uterus and allantois are highly vascularized. The allantois occupies most of the extraembryonic coelom and at the abembryonic pole, it contacts the omphaloplacenta and form the omphalallantoic placenta. This is the first description of all known placenta types in Squamata for a snake species member of the subfamily Colubrinae; where an eggshell membrane with 2.9 μm in width present throughout development is also evident. The structure of extraembryonic membranes in C. lineata is similar to that of other oviparous and viviparous squamate species. The above indicates not only homology, but also that the functional characteristics have been maintained throughout the evolution of the reproductive type.

Enhancing diffuser performance using transverse grooves to delay flow separation

A flow-control method is applied to enhance the efficiency and flow homogeneity of three-dimensional diffusers used in open-jet wind tunnels. Suitably shaped grooves are introduced in the diffuser diverging walls. The grooves promote the relaxation of the non-slip condition along the streamlines bounding the small recirculation regions forming passively inside the grooves. That reduces momentum losses and results in a downstream boundary layer with higher momentum, which is more separation-resistant. The proposed flow-control device has been successfully validated for plane diffusers [Mariotti et al., “Separation control and efficiency improvement in a 2D diffuser by means of contoured cavities,” Eur. J. Mech.-B 41, 138–149 (2013); and Mariotti et al., “Control of the turbulent flow in a plane diffuser through optimized contoured cavities,” Eur. J. Mech.-B 48, 254–265 (2014)]. In this study, we examined circular and square-section diffusers with different degrees of flow separation. Given that the investigated diffusers are part of open-jet wind tunnels, the entire wind tunnel geometry was included in the numerical simulation. The grooves significantly enhanced performance in circular diffusers by reducing the extent of separation and promoting an axisymmetric and spatially uniform flow. However, negligible benefits were observed for square-section diffusers. In these cases, since flow separation originates from one of the four inclined edges of the diffuser, placing grooves along the diverging walls does not effectively reduce the separation extent. Nonetheless, the grooves become effective again in diffusers with rectangular cross sections of high aspect ratio.

A double-sided 3D trench electrode detector using an 8-inch CMOS process: 3D simulation and experimental investigation

A double-sided 3D trench electrode detector (DS-3DTED) structure is proposed in this work to investigate the manufacturing process implementation of 3D detectors for high-energy physics, x-ray spectroscopy and x-ray cosmology applications. The device's electrical characterization, including electrostatic potential and electric field distributions, I–V, C–V, full depletion voltage and transient current with x-ray incidence, was performed with Synopsys® Sentaurus TCAD tools. In addition, a manufacturing method to realize the DS-3DTED device is presented. A 311 μm deep trench has been achieved through the Bosch process on the IMECAS 8-inch CMOS platform to verify the feasibility of the device structure. The maximum depth-to-width ratio is close to 105:1 when the trench width is 2 μm, which is an excellent foundation for manufacturing future 3D detector with a large fill factor and small dead region.

Functional characterization of a nanobody-based glycoprotein VI-specific platelet agonist

BackgroundGlycoprotein (GP)VI is a platelet-specific collagen receptor required for platelet activation during hemostasis. Platelet reactivity toward collagen is routinely assessed during diagnostic workup of platelet disorders. GPVI can be activated by inducing receptor clustering with suspensions of fibrillar collagen or synthetic cross-linked collagen-related peptide (CRP-XL). However, these suspensions are poorly standardized or difficult to produce. Nanobodies are small recombinant camelid-derived heavy-chain antibody variable regions. They are highly stable, specific, and ideal candidates for developing a stable GPVI agonist for diagnostic assays. ObjectivesDevelop a stable nanobody-based GPVI agonist. MethodsNanobody D2 (NbD2) was produced as dimers and purified. Tetramers were generated via C-terminal fusion of dimers with click chemistry. Nanobody constructs were functionally characterized with light transmission aggregometry (LTA) in platelet-rich plasma and whole blood flow cytometry. Diagnostic performance was assessed in patients with inherited platelet function disorders with LTA and flow cytometry. ResultsNbD2 was specific for human platelet GPVI. Dimers did not result in platelet activation in LTA or flow cytometry settings and fully inhibited CRP-XL-induced P-selectin expression and fibrinogen binding in whole blood and attenuated collagen-induced platelet aggregation in platelet-rich plasma. However, NbD2 tetramers caused full platelet aggregation, as well as P-selectin expression and fibrinogen binding. NbD2 tetramers were able to discriminate between inherited platelet function disorder patients and healthy controls based on fibrinogen binding, similar to CRP-XL. ConclusionNanobody tetramers to GPVI induce platelet activation and can be used to assess the GPVI pathway in diagnostic assays.

The host of GRB 171205A in 3D

Long GRB hosts at z < 1 are usually low-mass, low-metallicity star-forming galaxies. Here we present the most detailed, spatially resolved study of the host of GRB 171205A so far, a grand-design barred spiral galaxy at z = 0.036. Our analysis includes MUSE integral field spectroscopy complemented with high-spatial-resolution UV/VIS HST imaging and CO(1−0) and H I 21 cm data. The GRB is located in a small star-forming region in a spiral arm of the galaxy at a deprojected distance of ∼8 kpc from the center. The galaxy shows a smooth negative metallicity gradient and the metallicity at the GRB site is half solar, slightly below the mean metallicity at the corresponding distance from the center. Star formation in this galaxy is concentrated in a few H II regions between 5 and 7 kpc from the center and at the end of the bar, inwards from the GRB region; however the H II region hosting the GRB is in the top 10% of the regions with the highest specific star-formation rate. The stellar population at the GRB site has a very young component (< 5 Myr) that contributes a significant part of the light. Ionized and molecular gas show only minor deviations at the end of the bar. A parallel study found an asymmetric H I distribution and some additional gas near the position of the GRB, which might explain the star-forming region of the GRB site. Our study shows that long GRBs can occur in many types of star-forming galaxies; however the actual GRB sites have consistently low metallicity, high star formation rates, and a young population. Furthermore, gas inflow or interactions triggering the star formation producing the GRB progenitor might not be evident in ionized or even molecular gas but only in H I.

The complete chloroplast genome of Phyllostachys aureosulcata McClure (Bambusoideae: Poaceae): Comparative genomics and phylogenetic analysis

Phyllostachys aureosulcata McClure is a resilient bamboo species that is cultivated primarily as an ornamental plant in Beijing, Henan, Jiangsu, and Zhejiang, China. In this study, we sequenced and de novo assembled its complete chloroplast genome. The size of the complete genome was 139,701 base pairs (bp), which contained a large single-copy region of 83,223 bp, a small single-copy region of 12,882 bp, and a pair of inverted repeats of 21,798 bp each. Comparative analyses with 29 other species, including 17 from the genus Phyllostachys Torr., eight from the subfamily Bambusoideae, and four from the family Poaceae, revealed that Phyllostachys has a highly conserved chloroplast genome, showing similarities with other species within the subfamily and family. All analyzed genomes contained 111 unique genes, including 30 tRNAs, 4 rRNAs, and 77 protein-coding genes. Notably, the ycf1, ycf2, and accD genes were absent in all species of the family Poaceae. Phylogenetic reconstruction among 70 species of Bambusoideae, representing three tribes—Arundinarie, Bambuseae, and Olyreae—confirmed the monophyly of Phyllostachys, with a sister relationship between Phyllostachys aureosulcata and Phyllostachys propinqua McClure. The chloroplast genome-based phylogeny also revealed a sister relationship between the tribes Bambuseae and Olyreae, with their shared node rooted by Arundinarie. The comprehensive analysis of chloroplast genomes provides valuable insights into the comparative genomics and phylogenetics of Phyllostachys and the Bambusoideae subfamily.

Geosite Kahuripan Evaluation as Gili Genting Potential Asset for Archipelagic Geotourism Development

Abstract There is a growing global interest in natural tourism, which presents new opportunities for the development of small island regions. Several studies have shown that the development of tourism in the Indonesian Archipelago has led to several significant issues, so within the eastern islands of Sumenep— including Gili genting. The richness of natural features and the unique Maduranese Archipelago community, shown by Kahuripan area. However, the potential for developing a nature-based tourism sector (geosite) with the richness of geotourism, has not been adequately considered yet. Therefore, this study aimed to: 1) conducting inventory of the educational geotourism potential; 2) carrying out evaluations using GAM; 3) developing recommendations for future development of Gili Genting Potential Asset as Archipelagic Geotourism Development. According to inventory analysis, the Kahuripan Geosite consists of six Stop site with various information. The MV assessed 4.25 and AV counted of 4.75. The GAM diagram results indicate that the Kahuripan Geosite lies on Q22 tend to be moderate class of MV and AV. This quadrant suggests that the physical aspects of the geosites are quite interesting, while the tourism management that has begun to be developed needs to be further elaborated towards the geotourism-based education concept.

Effect of infusion direction on convection-enhanced drug delivery to anisotropic tissue.

Convection-enhanced delivery (CED) can effectively overcome the blood-brain barrier by infusing drugs directly into diseased sites in the brain using a catheter, but its clinical performance still needs to be improved. This is strongly related to the highly anisotropic characteristics of brain white matter, which results in difficulties in controlling drug transport and distribution in space. In this study, the potential to improve the delivery of six drugs by adjusting the placement of the infusion catheter is examined using a mathematical model and accurate numerical simulations that account simultaneously for the interstitial fluid (ISF) flow and drug transport processes in CED. The results demonstrate the ability of this direct infusion to enhance ISF flow and therefore facilitate drug transport. However, this enhancement is highly anisotropic, subject to the orientation of local axon bundles and is limited within a small region close to the infusion site. Drugs respond in different ways to infusion direction: the results of our simulations show that while some drugs are almost insensitive to infusion direction, this strongly affects other compounds in terms of isotropy of drug distribution from the catheter. These findings can serve as a reference for planning treatments using CED.

The propagation of negative pulsed discharges facilitated by artificially injected gas bubble in water

Abstract The artificial injection of bubbles into the electrode gap can effectively enhance the performance of underwater pulsed spark discharge (UPSD). It is crucial to investigate the morphology and propagation characteristics of discharges for a comprehensive understanding of bubble-stimulated UPSD. This paper presented an extensive study of negative discharges facilitated by the injected large bubble (with a diameter of 3 mm) in UPSD. The bridging effect of the bubble and the morphology and propagation of discharges were observed through the shadowgraph images captured by a high-speed camera. A numerical model was built to analyse the influence of the bubble on the initial electric field distribution between the electrodes. The characteristics of discharges were notably influenced by bubble parameters and energization conditions. Not in all cases could the bubble play the bridging effect role. The effective range of the bubble was roughly confined to a small region near the HV electrode tip. The experimental results obtained in this paper have certain guiding significance for the practical application of UPSD.

Causes and Influence of Regional Center Change in Small Region - As an Example of the Unju-myeon in Jeonbuk -

Causes and Influence of Regional Center Change in Small Region - As an Example of the Unju-myeon in Jeonbuk -

Algorithm 1046: An Improved Recurrence Method for the Scaled Complex Error Function

Calculation of the scaled complex error function \(w(z)\) by recurrence is discussed, and a new method for determining the number of steps required to achieve a given accuracy is introduced. This method is found to work throughout the complex plane, except for a short section of the real line, centred at the origin. An algorithm based on this analysis is implemented; Taylor series with stored coefficients are used to compute \(w(z)\) in a small region where recurrence is not efficient. The new algorithm is tested extensively and found to outperform earlier recurrence-based codes. It also performs favourably against recent codes based on other methods.

Characterization and phylogenetic analysis of the chloroplast genome in Elaeocarpus duclouxii

Elaeocarpus duclouxii, an evergreen tree species, is renowned for its fruits rich in flavonoids exhibiting potent antioxidant properties. Despite its significance, the chloroplast genome of this plant has remained unexplored until now. Our study presents the first comprehensive sequencing and analysis of the E. duclouxii chloroplast genome, revealing a circular DNA molecule of 158,148 base pairs. This genome comprises a large single-copy region of 85,700 base pairs, a small single-copy region of 17,672 base pairs, and a pair of inverted repeat regions totaling 27,388 base pairs. The genome encodes 132 genes, including 87 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. Phylogenetic analyses indicate a close evolutionary relationship between E. duclouxii and E. sylvestris. This study not only represents the first phylogenetic investigation of E. duclouxii but also establishes a crucial genomic foundation for future research area such as conservation genetics, evolutionary biology, and potential biotechnological applications.