Novice drivers often face challenges such as misjudgment, inappropriate steering control, distraction, and insufficient speed control when making left turns at intersections, leading to safety hazards. Installing intersection guide lines offers a solution by providing clear path directions, mitigating safety concerns associated with novice drivers’ left-turn actions. This study explored the impact of intersection guide line configurations on the driving behavior of novice drivers during left turns, utilizing large, medium, and small typical intersections to create six categories of left-turn simulation scenarios in a driving simulator. Data on vehicle trajectory, steering angle, steering speed, and eye-tracking were collected and analyzed. The study revealed that guide line arrangement significantly influences novice drivers’ left-turning behavior, enhancing path guidance while reducing trajectory and steering angle fluctuations, speed variations, and driver attention dispersion. This improvement in stability is particularly notable as intersection size and the number of left-turn lanes increase. The study’s findings offer robust theoretical support and guidance for the development and widespread adoption of intersection guide lines.