Small Channels Research Articles

Discharge Formula and Hydraulics of Rectangular Side Weirs in the Small Channel and Field Inlet

In this study, experimental investigations were conducted on rectangular side weirs with different widths and heights. Corresponding simulations were also performed to analyze hydraulic characteristics including the water surface profile, flow velocity, and pressure. The relationship between the discharge coefficient and the Froude number, as well as the ratios of the side weir height and width to upstream water depth, was determined. A discharge formula was derived based on a dimensional analysis. The results demonstrated good agreement between simulated and experimental data, indicating the reliability of numerical simulations using FLOW-3D software (version 11.1). Notably, significant fluctuations in water surface profiles near the side weir were observed compared to those along the center line or away from the side weir in the main channel, suggesting that the entrance effect of the side weir did not propagate towards the center line of the main channel. The proposed discharge formula exhibited relative errors within 10%, thereby satisfying the flow measurement requirements for small channels and field inlets.

Small Channels Assembled by Multilayer ZIF-8 in Nanocomposite Membranes for Filtration of Ofloxacin in Water.

Metal-organic framework (MOF)-based hybrid membranes still face many unsolved difficulties in the field of liquid separation, with a reliable production technique standing out, in particular, for the water-stable MOF membranes. In this study, zeolitic imidazolate framework-8 (ZIF-8) with acceptable water stability, favorable polymer affinity, and high selectivity was meticulously grafted on commercial poly(vinylidene fluoride) (PVDF) via substrate carboxylation-assisted etching and then overlaid onto PVDF to fabricate a novel hybrid membrane by a layer-by-layer self-assembly method. The optimal membrane manufacturing conditions, including assembly time (10 min), Hmim/Zn2+ molar ratio (8:1), and optimal layer number (three layers), were thoroughly investigated for cutting-off ofloxacin in water filtration. Under low pressure, a nanofiltration scale permeability of about 199.2 L m-2 h-1 MPa-1 and 97.9% rejection of ofloxacin were obtained in bench-scale tests based on the synergistic effect of the Donnan effect and steric hindrance. More significantly, the resulting hybrid membrane demonstrated excellent hydrophilicity, high antifouling, and mechanical and repeatability performances, suggesting promising application possibilities in real-world wastewater filtering settings.

Theoretical study of the O(3P) + CN(X2Σ+) → CO(X1Σ+) + N(2D)/N(4S) reactions.

The barrierless exothermic reactions between atomic oxygen and the cyano radical, O(3P) + CN(X2Σ+) → CO(X1Σ+) + N(2D)/N(4S), play a significant role in combustion, astrochemistry, and hypersonic environments. In this work, their dynamics and kinetics are investigated using both wave packet (WP) and quasi-classical trajectory (QCT) methods on recently developed potential energy surfaces of the 12A', 12A,″ and 14A″ states. The product state distributions in the doublet pathway obtained with the WP method for a few partial waves show extensive internal excitation in the CO product. This observation, combined with highly oscillatory reaction probabilities, signals a complex-forming mechanism. The statistical nature of the reaction is confirmed by comparing the WP results with those from phase space theory. The calculated rate coefficients using the WP (with a J-shifting approximation) and QCT methods exhibit agreement with each other near room temperature, 1.77 × 10-10 and 1.31 × 10-10cm3molecule-1s-1, but both are higher than the existing experimental results. The contribution of the quartet pathway is small at room temperature due to a small entrance channel bottleneck. The QCT rate coefficients are further compared with experimental results above 3000K, and the agreement is excellent.

Changes in the expression of satellite glial cell-specific markers during postnatal development of rat sympathetic ganglia

The sympathetic ganglia represent a final motor pathway that mediates homeostatic “fight and flight” responses in the visceral organs. Satellite glial cells (SGCs) form a thin envelope close to the neuronal cell body and synapses in the sympathetic ganglia. This unique morphological feature suggests that neurons and SGCs form functional units for regulation of sympathetic output. In the present study, we addressed whether SGC-specific markers undergo age-dependent changes in the postnatal development of rat sympathetic ganglia. We found that fatty acid-binding protein 7 (FABP7) is an early SGC marker, whereas the S100B calcium-binding protein, inwardly rectifying potassium channel, Kir4.1 and small conductance calcium-activated potassium channel, SK3 are late SGC markers in the postnatal development of sympathetic ganglia. Unlike in sensory ganglia, FABP7 + SGC was barely detectable in adult sympathetic ganglia. The expression of connexin 43, a gap junction channel gradually increased with age, although it was detected in both SGCs and neurons in sympathetic ganglia. Glutamine synthetase was expressed in sensory, but not sympathetic SGCs. Unexpectedly, the sympathetic SGCs expressed a water-selective channel, aquaporin 1 instead of aquaporin 4, a pan-glial marker. However, aquaporin 1 was not detected in the SGCs encircling large neurons. Nerve injury and inflammation induced the upregulation of glial fibrillary acidic protein, suggesting that this protein is a hall marker of glial activation in the sympathetic ganglia. In conclusion, our findings provide basic information on the in vivo profiles of specific markers for identifying sympathetic SGCs at different stages of postnatal development in both healthy and diseased states.

Evaluating the Impact of Using Multiple-Metal Layers on the Layout Area of Switch Blocks for Tile-Based FPGAs in FinFET 7nm

A new area model for estimating the layout area of switch blocks is introduced in this work. The model is based on a realistic layout strategy. As a result, it not only takes into consideration the active area that is needed to construct a switch block but also the number of metal layers available and the actual dimensions of these metals. The model assigns metal layers to the routing tracks in a way that reduces the number of vias that are needed to connect different routing tracks together while maintaining the tile-based structure of FPGAs. It also takes into account the wiring area required for buffer insertion for long wire segments. The model is evaluated based on the layouts constructed in the ASAP7 FinFET 7nm Predictive Design Kit. We found that the new model, while specific to the layout strategy that it employs, improves upon the traditional active-based area estimation models by considering the growth of the metal area independently from the growth of the active area. As a result, the new model is able to more accurately estimate the layout area by predicting when the metal area will overtake the active area as the number of routing tracks is increased. This ability allows the more accurate estimation of the true layout cost of FPGA fabrics at the early floor planning and architectural exploration stage; and this increase in accuracy can encourage a wider use of custom FPGA fabrics that target specific sets of benchmarks in future SOC designs. Furthermore, our data indicate that the conclusions drawn from several significant prior architectural studies remain to be correct under FinFET geometries and wiring area considerations despite their exclusive use of active-only area models. This correctness is due to the small channel widths, around 30–60 tracks per channel, of the architectures that these studies investigate. For architectures that approach the channel width of modern commercial FPGAs with more than 100–200 tracks per channel, our data show that wiring area models justified by detailed layout considerations are an essential addition to active area models in the correct prediction of the implementation area of FPGAs.

Comparison of CFD-DEM and MP-PIC in the Simulation of Metal Powder Conveying for Laser Metal Deposition

Pneumatic conveying of fine powders is essential for many industrial processes, including Laser Metal Deposition (LMD), a Direct Metal Additive Manufacturing (DMAM) technology that builds solid objects layer-by-layer using a laser to melt metal powder. To optimize the process, it is necessary to have a correct understanding of the powder’s behaviour under the process condition. The coupled Computational Fluid Dynamics - Discrete Element Modelling (CFD-DEM) and MultiPhase - Particle In Cell (MP-PIC) are two popular Eulerian-Lagrangian models to simulate particle laden flows. This study compares them to analyse powder behaviour in a small channel of LMD machines. Results from the two methods differ significantly, with CFD-DEM offering a more accurate representation of the physical reality, while MP-PIC is more computationally efficient. The study finds that the CFD-DEM method produces higher fluctuations in the solids flow rate due to the formation of particle clusters, while MP-PIC displays a smooth and essentially uniform flow. The results suggest that CFD-DEM should be used for more accurate and detailed studies of solids flow rate in pneumatic conveying systems, while MP-PIC can be used for preliminary studies and design optimization.

The role of GpsB in Staphylococcus aureus cell morphogenesis.

Staphylococcus aureus is a Gram-positive clinical pathogen, which is currently the second cause of death by antibiotic-resistant infections worldwide. For decades, S. aureus cells were thought to be spherical and lack the ability to undergo elongation. However, super-resolution microscopy techniques allowed us to observe the minor morphological changes that occur during the cell cycle of this pathogen, including cell elongation. S. aureus elongation is not required for normal growth in laboratory conditions. However, it seems to be essential in the context of some infections, such as osteomyelitis, during which S. aureus cells apparently elongate to invade small channels in the bones. In this work, we uncovered new determinants required for S. aureus cell elongation. In particular, we show that GpsB has an important role in the spatio-temporal regulation of PBP2 and PBP4, two proteins involved in peptidoglycan synthesis, contributing to the maintenance of the correct cell morphology in S. aureus.

Early efficacy observation of the unilateral biportal endoscopic technique in the treatment of multi-level lumbar spinal stenosis

BackgroundTo explore the early curative effect of unilateral biportal endoscopy (UBE) in the treatment of multi-level lumbar spinal stenosis with the help of multiple small incisions.MethodsA retrospective analysis was performed on 26 patients with multi-level lumbar spinal stenosis treated by UBE in our hospital from August 1, 2021, to March 1, 2022. We collect patients’ basic medical records and independently design surgical incisions. The visual analog score (VAS) and Oswestry Disability Index (ODI) were compared before surgery, 7 days after surgery and 6 months after surgery. Spinal canal diameters on CT were compared before surgery and 7 days after surgery. The modified MacNab standard was used to evaluate the efficacy satisfaction at 6 months after operation.ResultsIn this study, 26 patients were operated according to the predetermined surgical plan. The operative time was 145 ± 40.11 min, the intraoperative blood loss was 156.25 ± 44.32 ml, and the postoperative hospital stay was 4.79 ± 1.31 days. The VAS scores of postoperative lumbago and leg pain were lower than those before surgery (P < 0.05). The postoperative ODI score was significantly different from that before surgery (P < 0.05). The postoperative CT sagittal diameter was significantly different from that before surgery (P < 0.05). The curative effect of modified MacNab was 76.92% when followed up 7 days after surgery. The curative effect of modified MacNab was 92.31% when followed up 6 months after surgery, which was significantly improved compared with 7 days after surgery.ConclusionUnder multiple small incision channels, UBE can effectively treat multi-level lumbar spinal stenosis, significantly relieve the clinical symptoms of patients, and significantly improve the quality of life of patients. It is a safe and feasible minimally invasive surgical treatment method for multi-level lumbar spinal stenosis.

Lq-norm multiple kernel fusion regression for self-cleansing sediment transport

Experimental and modeling studies have been conducted to develop an approach for self-cleansing rigid boundary open channel design such as drainage and sewer systems. Self-cleansing experiments in the literature are mostly performed on circular channel cross-section, while a few studies considered self-cleansing sediment transport in small rectangular channels. Experiments in this study were carried out in a rectangular channel with a length of 12.5 m, a width of 0.6 m, a depth of 0.7 m and having an automatic control system for regulating channel slope, discharge and sediment rate. Behind utilizing collected experimental data in this study, existing data in the literature for rectangular channels are used to develop self-cleansing models applicable for channel design. Through the modeling procedure, this study recommends Lq-norm multiple kernel fusion regression (LMKFR) techniques for self-cleansing sediment transport. The LMKFR is a regression technique based on the regularized kernel regression method which benefits from the combination of multiple information sources to improve the performance using the Lq-norm multiple kernel learning framework. The results obtained by LMKFR are compared to support vector regression benchmark and existing conventional regression self-cleansing sediment transport models in the literature for rectangular channels. The superiority of LMKFR is illustrated in an accurate modeling as compared with its alternatives in terms of various statistical error measurement criteria. The encouraging results of LMKFR can be linked to utilization of several kernels which are fused effectively using an Lq-norm prior that captures the intrinsic sparsity of the problem at hand. Promising performance of LMKFR technique in this study suggests it as an effective technique to be examined in similar environmental, hydrological and hydraulic problems.

Implications of bioturbation induced by Procambarus clarkii on seepage processes in channel levees

River levees are subject to bioturbation by various animals which can actively excavate into earthen structures producing an internal erosion that, during the passage of a flood, can grow in time making the levee unstable. This phenomenon can lead to river levee breaching and, as a consequence, collapse, even for relatively minor flood events. A well-known animal burrower is represented by the North American crayfish Procambarus clarkii (P. clarkii), an invasive species in Europe, mainly introduced for commercial purposes, causing a decline in biodiversity and profound habitat changes. The physical damages caused by P. clarkii on levees and banks, such as in rice fields, irrigation ditches, and small channels, have not been fully studied and behavioral components underlying this impact are mostly occasional. To understand the impact of burrowing activity on the seepage process, a field survey was done in a drainage channel in Tuscany, Italy, to evaluate the density and geometry of the internal burrows that were excavated by the crayfish. Based on these observations and some previous laboratory experiments, three dimensional (3D) numerical simulations of the seepage processes were done inside burrowed levees. Numerical results allowed the increase in the hydraulic vulnerability of levees to the process of internal seepage to be disclosed. In particular, for a given river water level, the reduction of the time scale for the phreatic line to reach the levee field side appears to be a function of a quantity here defined as the burrow hydraulic gradient. This quantity is here defined as the ratio between the hydraulic head inside the burrow and the horizontal distance from its end to the field side of the levee. Moreover, a comparison between the 3D with the analogous more common two dimensional (2D) numerical simulations illustrated the schematization which is better suited for describing the seepage processes when animal burrows, not only by crayfish, are present.

LC Tank Oscillator Based on New Negative Resistor in FDSOI Technology

Although Moore’s Law reaches its limits, it has never applied to analog and RF circuits. For example, due to the short channel effect (SCE), drain-induced barrier lowering (DIBL), and sub-threshold slope (SS)…, longer transistors are required to implement analog cells. From 22 nm CMOS technology and beyond, for reasons of variability, the channel of the transistors has no longer been doped. Two technologies then emerged: FinFET transistors for digital applications and UTBB FDSOI transistors, suitable for analog and mixed applications. In a previous paper, a new topology was proposed utilizing some advantages of the FDSOI technology. Thanks to this technology, a novel cross-coupled back-gate (BG) technique was implemented to improve analog and mixed signal cells in order to reduce the surface of the integrated circuit. This technique was applied to a current mirror to reduce the small channel effect and to provide high-output impedance. It was demonstrated that it is possible to overcompensate the SCE and DIBL effects and to create a negative output resistor. This paper presents a new LC tank oscillator based on this current mirror functioning as a negative resistor.

Role of Protein Kinase C in Metabolic Regulation of Coronary Endothelial Small Conductance Calcium-Activated Potassium Channels.

Small conductance calcium-activated potassium (SK) channels are largely responsible for endothelium-dependent coronary arteriolar relaxation. Endothelial SK channels are downregulated by the reduced form of nicotinamide adenine dinucleotide (NADH), which is increased in the setting of diabetes, yet the mechanisms of these changes are unclear. PKC (protein kinase C) is an important mediator of diabetes-induced coronary endothelial dysfunction. Thus, we aimed to determine whether NADH signaling downregulates endothelial SK channel function via PKC. SK channel currents of human coronary artery endothelial cells were measured by whole cell patch clamp method in the presence/absence of NADH, PKC activator phorbol 12-myristate 13-acetate, PKC inhibitors, or endothelial PKCα/PKCβ knockdown by using small interfering RNA. Human coronary arteriolar reactivity in response to the selective SK activator NS309 was measured by vessel myography in the presence of NADH and PKCβ inhibitor LY333531. NADH (30-300 μmol/L) or PKC activator phorbol 12-myristate 13-acetate (30-300 nmol/L) reduced endothelial SK current density, whereas the selective PKCᵦ inhibitor LY333531 significantly reversed the NADH-induced SK channel inhibition. PKCβ small interfering RNA, but not PKCα small interfering RNA, significantly prevented the NADH- and phorbol 12-myristate 13-acetate-induced SK inhibition. Incubation of human coronary artery endothelial cells with NADH significantly increased endothelial PKC activity and PKCβ expression and activation. Treating vessels with NADH decreased coronary arteriolar relaxation in response to the selective SK activator NS309, and this inhibitive effect was blocked by coadministration with PKCβ inhibitor LY333531. NADH-induced inhibition of endothelial SK channel function is mediated via PKCβ. These findings may provide insight into novel therapeutic strategies to preserve coronary microvascular function in patients with metabolic syndrome and coronary disease.

HiPSC-derived cardiomyocytes as a model to study the role of small-conductance Ca2+-activated K+ (SK) ion channel variants associated with atrial fibrillation.

Atrial fibrillation (AF), the most common arrhythmia, has been associated with different electrophysiological, molecular, and structural alterations in atrial cardiomyocytes. Therefore, more studies are required to elucidate the genetic and molecular basis of AF. Various genome-wide association studies (GWAS) have strongly associated different single nucleotide polymorphisms (SNPs) with AF. One of these GWAS identified the rs13376333 risk SNP as the most significant one from the 1q21 chromosomal region. The rs13376333 risk SNP is intronic to the KCNN3 gene that encodes for small conductance calcium-activated potassium channels type 3 (SK3). However, the functional electrophysiological effects of this variant are not known. SK channels represent a unique family of K+ channels, primarily regulated by cytosolic Ca2+ concentration, and different studies support their critical role in the regulation of atrial excitability and consequently in the development of arrhythmias like AF. Since different studies have shown that both upregulation and downregulation of SK3 channels can lead to arrhythmias by different mechanisms, an important goal is to elucidate whether the rs13376333 risk SNP is a gain-of-function (GoF) or a loss-of-function (LoF) variant. A better understanding of the functional consequences associated with these SNPs could influence clinical practice guidelines by improving genotype-based risk stratification and personalized treatment. Although research using native human atrial cardiomyocytes and animal models has provided useful insights, each model has its limitations. Therefore, there is a critical need to develop a human-derived model that represents human physiology more accurately than existing animal models. In this context, research with human induced pluripotent stem cells (hiPSC) and subsequent generation of cardiomyocytes derived from hiPSC (hiPSC-CMs) has revealed the underlying causes of various cardiovascular diseases and identified treatment opportunities that were not possible using in vitro or in vivo studies with animal models. Thus, the ability to generate atrial cardiomyocytes and atrial tissue derived from hiPSCs from human/patients with specific genetic diseases, incorporating novel genetic editing tools to generate isogenic controls and organelle-specific reporters, and 3D bioprinting of atrial tissue could be essential to study AF pathophysiological mechanisms. In this review, we will first give an overview of SK-channel function, its role in atrial fibrillation and outline pathophysiological mechanisms of KCNN3 risk SNPs. We will then highlight the advantages of using the hiPSC-CM model to investigate SNPs associated with AF, while addressing limitations and best practices for rigorous hiPSC studies.

Thermal and Visualisation Study of the HFE7100 Refrigerant Condensation Process

Abstract Technological advances are contributing to the search for highly efficient energy designs, and increasing interest in compact heat exchangers. Indeed, small channel diameters determine large heat transfer coefficients and condition a significant heat transfer area about the overall volume of the heat exchanger, as well as a smaller amount of refrigerant flowing in the system. Nevertheless, the operating stability and energy efficiency of compact heat exchangers are influenced by two-phase flow structures, which depend on thermal flow parameters. Knowledge of the structures formed during the condensation process is therefore essential for optimising the operation of refrigeration and air-conditioning equipment. This article presents the results from experimental studies of the HFE7100 refrigerant, from the hydrofluorocarbon group, condensation process in mini-channels with hydraulic diameters dh = 2.0 mm, 1.2 mm, 0.8 mm and 0.5 mm. Thermal flow characteristics were determined, and the forming structures of two-phase flow were recorded. The results of visualisation were subjected to morphological image analysis, based on a special algorithm written in MATLAB software. The algorithm makes it possible to determine the void fraction, which is necessary for calculating the vapour quality, as well as the area of vapour bubbles and their number, directionality and length along the x- and y-axes.

Negative effects of agricultural open-channel irrigation system on vertebrate populations in central Mexico.

Linear infrastructures such as agricultural irrigation channels produce physical changes and negative impacts to habitats, wildlife populations, communities, and ecosystems. Open irrigation channels act as a pitfall for wildlife and can affect vertebrates of all sizes. Nonetheless, small channels have received relatively little attention by conservation biologists. The objective of this study was to analyze vertebrate species richness and mortality in relation to different sections of an irrigation channel system and the surrounding landscape characteristics. For two years, we conducted monthly surveys along an open-channel irrigation system to estimate its effect on vertebrates through records of dead and alive individuals. We examined the spatial relation of species richness and mortality with transects using a canonical correspondence analysis and chi-squared tests to determine possible variations in the different structures of the channel and seasonality. Further, a landscape diversity index was used to analyze the importance of surrounding habitat structure and composition on these parameters. Most vertebrates (61%) were found dead, small mammals and reptiles were the most affected. Our results indicate that mortality of small vertebrates varies depending on species, structures of the open-channel agricultural irrigation system (i.e., concrete channel and floodgates), seasonality (i.e., wet, and dry), and landscape heterogeneity (i.e., high, medium, and low landscape diversity). The open-channel irrigation system is a threat to populations of small vertebrates in anthropized landscapes, conservation efforts should be directed at protecting water bodies and restructuring the open-channel agricultural irrigation system to avoid mortality of species such as small rodents (M. mexicanus) and reptiles (C. triseriatus, B. imbricata, and Thamnophis spp.).

Fast computation approach of electron-impact ionization and excitation cross-sections for atoms and ions with medium- and high-&lt;i&gt;Z&lt;/i&gt; elements

The atomic data of medium- and high-&lt;i&gt;Z&lt;/i&gt; elements, such as electron-impact ionization and excitation cross-sections, possess extensive applications in fields such as fusion science and X-ray interactions with matter. There are atoms and ions in high energy density plasma, with different charge states and energy states ranging from ground states to highly excited states, and the cross-sections of each charge state and energy state need to be calculated. The bottlenecks limiting computational performance are the inevitable relativistic effects of medium- and high-&lt;i&gt;Z&lt;/i&gt; elements and the extremely complex electronic configurations. Taking tantalum (Ta) for example, by using the relativistic Dirac-Fock theory and distorted wave model, we compute the electron-impact ionization and excitation cross-sections of Ta from the ground state atom up to Ta&lt;sup&gt;72+&lt;/sup&gt; with the incident electron energy range of 1–150 keV. The detailed configuration accounting (DCA) reaction channel cross-sections are derived by summing and weighting the original detailed level accounting (DLA) cross-sections. After examining the data, two regularities are found. In terms of DLA, the pre-averaging DCA cross-sections have varying initial DLA energy levels but are typically close to each other. There is not a straightforward function that can explain the discrepancies between them. In terms of DCA, inner subshells typically contribute very little to the total cross-section as their ionization and excitation cross-sections are orders of magnitude smaller than those of outer subshells. We provide two techniques to reduce the computational costs based on the regularities. To minimize the total number of DLA reaction channels used in the computation, the initial DLA energy levels can be randomly sampled. Through a Monte Carlo numerical experiment, we determine the appropriate number of sampling points that can reduce the total number of DLA channels by an order of magnitude while maintaining a 5% error margin. In terms of impact ionization, since small cross-section DCA channels are insignificant, only a tiny portion of the DCA channels are required to preserve a 95% accuracy of the entire cross-section. It is possible to use the analytical Binary Encounter Bethe (BEB) formula to determine which DCA channels should be neglected before the computation to reduce computational costs. In terms of electron-impact excitation, just the cross-sections of the same excited subshells as the preserved ionized subshells, which are determined in the previous electron-impact ionization (EII) calculations, are needed. Finally, we compare our EII results with theoretical and experimental results. In the low incident electron energy range of below 2 keV, our results accord with the theoretical result of the 6s EII cross-section of the Ta atom and the experimental result of the total EII cross-section of the Ta&lt;sup&gt;1+&lt;/sup&gt; ion. In the high energy range of below 150 keV, our results are also consistent with the theoretical result of the 1s EII cross-section of the Ta atom and the experimental result of the 1s EII cross-section of the Cu atom. Our results reasonably match the previous experimental and theoretical results in low-energy range and high-energy range, inner subshell and outer subshell, indicating the accuracy of our calculation. The proposed optimizing strategy can be applied to various medium- to high-&lt;i&gt;Z&lt;/i&gt; elements and is compatible to most computation codes.

Roles and Mechanisms of Dopamine Receptor Signaling in Catecholamine Excess Induced Endothelial Dysfunctions.

Endothelial dysfunction may contribute to pathogenesis of Takotsubo cardiomyopathy, but mechanism underlying endothelial dysfunction in the setting of catecholamine excess has not been clarified. The study reports that D1/D5 dopamine receptor signaling and small conductance calcium-activated potassium channels contribute to high concentration catecholamine induced endothelial cell dysfunction. For mimicking catecholamine excess, 100 μM epinephrine (Epi) was used to treat human cardiac microvascular endothelial cells. Patch clamp, FACS, ELISA, PCR, western blot and immunostaining analyses were performed in the study. Epi enhanced small conductance calcium-activated potassium channel current (ISK1-3) without influencing the channel expression and the effect was attenuated by D1/D5 receptor blocker. D1/D5 agonists mimicked the Epi effect, suggesting involvement of D1/D5 receptors in Epi effects. The enhancement of ISK1-3 caused by D1/D5 activation involved roles of PKA, ROS and NADPH oxidases. Activation of D1/D5 and SK1-3 channels caused a hyperpolarization, reduced NO production and increased ROS production. The NO reduction was membrane potential independent, while ROS production was increased by the hyperpolarization. ROS (H2O2) suppressed NO production. The study demonstrates that high concentration catecholamine can activate D1/D5 and SK1-3 channels through NADPH-ROS and PKA signaling and reduce NO production, which may facilitate vasoconstriction in the setting of catecholamine excess.

In situ polymer gelation in confined flow controls intermittent dynamics.

Polymer flows through pores, nozzles and other small channels govern engineered and naturally occurring dynamics in many processes, from 3D printing to oil recovery in the earth's subsurface to a wide variety of biological flows. The crosslinking of polymers can change their material properties dramatically, and it is advantageous to know a priori whether or not crosslinking polymers will lead to clogged channels or cessation of flow. In this study, we investigate the flow of a common biopolymer, alginate, while it undergoes crosslinking by the addition of a crosslinker, calcium, driven through a microfluidic channel at constant flow rate. We map the boundaries defining complete clogging and flow as a function of flow rate, polymer concentration, and crosslinker concentration. Interestingly, the boundaries of the dynamic behavior qualitatively match the thermodynamic jamming phase diagram of attractive colloidal particles. That is, polymer clogging occurs in a region analogous to colloids in a jammed state, while the polymer flows in regions corresponding to colloids in a liquid phase. However, between the dynamic regimes of complete clogging and unrestricted flow, we observe a remarkable phenomenon in which the crosslinked polymer intermittently clogs the channel. This pattern of deposition and removal of a crosslinked gel is simultaneously highly reproducible, long-lasting, and controllable by system parameters. Higher concentrations of polymer and cross-linker result in more frequent ablation, while gels formed at lower component concentrations ablate less frequently. Upon ablation, the eluted gel maintains its shape, resulting in micro-rods several hundred microns long. Our results suggest both rich dynamics of intermittent flows in crosslinking polymers and the ability to control them.

Practice of methods for remote control of walking robotic mini-dredgers moving along the bottom

The results of experimental testing of methods for remote control of underwater walking mini-dredgers are discussed. Tests were carried out in real water conditions on the basis of a prototype of a 6-legged underwater walking vehicle. During the tests, technological operations for clearing and deepening small channels and eriks, as well as the extraction of sapropel from the bottom of the lake, were simulated. During the experiments, a mini-dredger has been controlled using video information of on-board video cameras. Due to poor visibility in the zone of hydraulic erosion of bottom soil, management was carried out in conditions of an incomplete and ambiguous understanding of the external environment. Local positioning was carried out according to natural landmarks. It is shown that fairly accurate remote control of the movement of a mini-dredger can be organized on the basis of public satellite maps of the area, even in conditions of a lack of sensory information. Moreover, the operation of an underwater dredger can be realized with minimal external control influences. The research results may be demanded in the development of walking robotic systems intended for the rehabilitation of degrading small water bodies and for other underwater technical work.

Review of peculiarities of using wind catchers in energy-saving ventilation systems

Natural ventilation plays an important role in ensuring comfortable living conditions. Wind catchers are part of this process and can provide the required ventilation rate for buildings. The performance of wind catchers depends on the relevant parameters, such as height, configuration, cross-sectional shape, number of inlets, speed of movement and variability of air movement direction. HVAC equipment is not only responsible for the largest portion of the total energy consumption within a building, but also for most indoor air quality problems. Vents, air ducts, and dirty filters are a suitable place for the growth of fungi and molds produced by organic dust, which contaminate the circulating air and cause significant pollution problems. This could be more critical given that we spend almost 90% of our time indoors during our lives and work. Improvements in ventilation and air conditioning systems therefore play an important role in increasing energy efficiency in buildings, providing a better indoor climate for occupants and, as a result, reducing the likelihood of health problems. The cooling process plays an important role in creating comfortable conditions for humans. One of the most well-known elements of a passive cooling system for buildings with no or minimal energy consumption is a wind catcher. The effective-ness of wind catchers is influenced by wind force and buoyancy, and knowledge of the size, shape, and position of the outlet will help to understand new ideas and technologies for their application in modern architecture. A well-known example of natural ventilation that improves indoor quality by reducing pollution and humidity by replacing stale air with fresh outside air is a wind catcher (or wind tower). Nowadays, wind catchers are widely used in the world, having advantages in densely populated urban areas and in areas with low wind speeds, significantly affecting the reduction of cooling loads and providing the required ventilation rate of buildings. At present, there are many types of wind traps that can be classified according to the number of inlets, cross-sectional shape, and number of levels. The number of internal partitions, as well as the size and location of the openings of the wind collector, significantly affect its ventilation characteristics, efficiency, air flow velocity and turbulence, and also divide the cross-section of the wind collector into smaller channels, increasing the strength of its structure and reducing sensitivity to different wind directions