Small Aggregates Research Articles

Spatial and Temporal Evolution and Conservation Significance of Traditional Villages in the Yangtze River Basin, China

Traditionally, “ancient villages” are communities that date back to a previous era, possess abundant traditional resources, and hold significant historical, social, cultural, ecological, and economic significance. This study examines the geographical and chronological development of 2957 traditional communities in the Yangtze River Basin and extensively employs GIS spatial analysis, geostatistical analysis, and historical literature review to study the evolution of the locations of traditional settlements under various factors. The study’s findings revealed that: (1) From a geographical standpoint, the traditional villages in the study area present a distribution state of “two cores, small aggregation, and many dispersions”. The two core clusters are located at the intersection of Hunan, Guizhou, and Chongqing provinces, where the natural geographical advantages attracted people from different dynasties to settle here. (2) From a temporal perspective, the quantity of traditional villages shows a growing trend, and the distribution center of each dynasty shows an east–west direction. Among them, the shift from the Song to the Yuan dynasties marked the period of greatest change in village distribution, which originated from the Song Dynasty’s quick economic growth. In addition, the village distribution was clustered in the eastern part of the country, adapting to the needs of commercial trade. When the Song Dynasty fell, the society was in turmoil, and the villages were transferred to the central region to avoid the war. The Yuan Dynasty also laid down the fundamental distribution pattern of conventional villages. (3) From the standpoint of choosing a village location, most of them are distributed in mountainous, hilly, and plain regions; the gentle slopes of 0°~15° are favored by people. Sufficient water sources, abundant precipitation, and a suitable climate are also necessary choices. (4) From the perspective of driving factors, traditional village distribution in the basin is impacted by historical, cultural, social, and ecological factors. Included among these, traditional village distribution is inversely connected with socio-economic variables. Under the interaction of two factors, the distribution will be more affected. To summarize, the complex human geographic factors together create the conventional village dispersal pattern, and examining these elements holds substantial practical importance for comprehending, safeguarding, and preserving traditional villages.

The effects of roots and earthworms on aggregate size distribution and their associated carbon under contrasting soil types and soil moisture conditions

Soil’s potential as a carbon sink is uncertain due to biotic and abiotic interactions. Soil aggregates are important carriers of organic carbon (OC), and clarifying the response of aggregates and their associated OC to plant roots and earthworms can better understand the carbon cycle in terrestrial ecosystems. Herein, we determined the regulatory role of plant roots (Lolium perenne) and earthworms (Metaphire tschiliensis) in isolation and in combination on aggregate size distribution and their associated OC and readily oxidizable OC (ROOC) under contrasting soil types (Phaeozems and Anthrosols) and soil moisture conditions (constant wetting, CW; wetting–drying cycle repeated four times, 4WD) by a laboratory microcosm experiment. Results showed that earthworm presence significantly increased plant biomass by 30 % and 221 % in Phaeozems and Anthrosols, respectively (p < 0.05). For Phaeozems, plant alone (P) and plant and earthworm in combination (PE) had lower proportion of large aggregate (5–8 and 2–5 mm) than bare soil (CK) under 4WD condition (p < 0.05). For Anthrosols, the PE treatment decreased the proportion of 5–8 and 2–5 mm aggregate by 45 % and 25 %, respectively, under CW condition, and decreased the proportion of 5–8 mm aggregate by 37 % under 4WD condition (p < 0.05). Moreover, the proportion of 5–8 and 2–5 mm aggregate in P treatment was lower under CW condition (6.92 % and 22.13 %) but was higher under 4WD condition (11.49 % and 32.55 %) than those in CK (14.69 % and 29.91 %; 8.85 % and 20.56 %) (p < 0.05). Our results further indicated that the PE treatment had relatively higher OC and ROOC contents, especially for small aggregates in Anthrosols. The study indicates that plant roots and earthworms significantly influence aggregate turnover and the carbon cycle, with soil type and moisture playing a crucial role.

Wax crystal dynamics and viscosity abnormal reduction in asphaltene-containing waxy oil at low temperatures

A micro-rheological integration experiment was conducted on model oils without asphaltene and with 0.05 wt% asphaltene. The study examined the viscosity-temperature characteristics of both model oils under different cooling rates and shear rates. Significant changes were observed in the viscosity-temperature curve of the asphaltene-containing model oil. At low shear rates, a distinct wavelet peak was identified in the temperature range of 37-30 °C, where viscosity initially increased, then decreased, and subsequently increased again. This wavelet peak diminished with the increasing shear rate, eventually forming a “buffer platform”. The underlying mechanism was attributed to asphaltene altering the wax crystal behavior from “uniform precipitation network structure formation” to “formation of unstable large aggregates - dissociation into multiple small stable aggregates - growth into larger stable aggregates.” In addition, the wax precipitation rate remained constant in the model oil without asphaltene, while in the asphaltene-containing model oil, the rate significantly accelerated between 34.5 °C and 32.5 °C. And the asphaltenes promoted the formation of small wax crystal particles but inhibited the growth of wax crystal particles.

Clinician-Ordered Peripheral Smear Review by a Pathologist Has Low Clinical Utility-A Reference Laboratory Perspective.

Clinician-ordered peripheral smear review by pathologist (CPSR) is commonly ordered and has been recommended for decades. However, the clinical utility of this labor-intensive test in the reference laboratory has not been examined. The objective of this study is to assess hematologic abnormalities identified in CPSR orders and to correlate them with complete blood count (CBC) and laboratory-derived smear review (LDSR) in the reference laboratory. Two hundred consecutive CPSRs with corresponding CBCs from April 2023 were run by Sysmex® XN-11 analyzers, and their peripheral smears were examined by a board-certified hematopathologist. Hematologic abnormalities of CPSRs were assessed, and the correlation between CPSR and CBC/LDSR was reviewed. Nearly one-third of CPSRs (29%) had normal peripheral smears and CBCs. The majority of CPSRs showed nonspecific quantitative abnormalities. When compared against CPSR results, LDSR criteria identified 100% of hematologic abnormalities appropriately. Samples that were not flagged for review by LDSR rules were also reviewed (n = 174) to rule out clinically meaningful false negatives. One minor discrepancy (0.6% of cases) of small platelet aggregates was observed in a patient with a reported platelet count of 139 K/uL that was missed by the LDSR process. In the reference laboratory setting, our findings demonstrate that LDSR adequately detects significant hematologic abnormalities and, therefore, CPSR should be discontinued.

Performance Assessment on the Manufacturing of Zn-22Al-2Cu Alloy Foams Using Barite by Melt Route

A barium-rich Celestine (Sr,Ba)SO4 concentrate from the primary Mexican ore production was used as a thickening agent to produce closed-cell Zn-22Al-2Cu alloy foams, while calcium carbonate was used as a foaming agent. The microstructure and mechanical properties of the foams were analyzed by optical microscopy, scanning electron microscopy, and compression tests, respectively. The Zn-22Al-2Cu alloy foams showed a typical lamellar eutectic microstructure, constituted by a zinc-rich phase (η) and a (α) solid solution that was richer in aluminum, while a copper-rich (ε) phase was formed in the interdendritic regions. The SEM micrographs show the presence of small particles and aggregates that are randomly scattered in the cell walls and correspond to unreacted calcite and Celestine–Barian particles, especially for the higher barite addition. The compressive curves showed smooth behavior, wherein the particles at the cell walls did not affect the foam’s compressive behavior. The trial containing 1.5 wt. % of BaSO4 and 1.0 wt. % of CaCO3 showed a higher energy absorption capacity of 5.64 MJ m−3 because of its highest relative density and lowest porosity values. The Celestine–Barian concentrate could be used as a foaming agent for high melt-point metals or alloys based on the TGA results.

Inhibition of Aβ16-22 Aggregation by [TEA]+[Ms]- Follows Weakening of the Hydrophobic Core and Sequestration of Peptides in Ionic Liquid Nanodomains.

We developed a coarse-grained model for the protic ionic liquid, triethylammonium mesylate ([TEA]+[Ms]-), to characterize its inhibitory effects on amyloid aggregation using the K16LVFFAE22 fragment of the amyloid-β (Aβ16-22) as a model amyloidogenic peptide. In agreement with previous experiments, coarse-grained molecular dynamics simulations showed that increasing concentrations of [TEA]+[Ms]- in aqueous media led to increasingly small Aβ16-22 aggregates with low beta-sheet contents. The cause of [TEA]+[Ms]-'s inhibition of peptide aggregation was found to be a result of two interrelated effects. At a local scale, the enrichment of interactions between [TEA]+ cations and hydrophobic phenylalanine side chains weakened the hydrophobic cores of amyloid aggregates, resulting in poorly ordered structures. At a global level, peptides tended to localize at the interfaces of IL-rich nanostructures with water. At high IL concentrations, when the IL-water interface was large or fragmented, Aβ16-22 peptides were dispersed in the simulation cell, sometimes sequestered at unaggregated monomeric states. Together, these phenomena underlie [TEA]+[Ms]-'s inhibition of amyloid aggregation. This work addresses the critical lack of knowledge on the mechanisms of protein-ionic liquid interactions and may have broader implications for industrial applications.

Green synthesis and characterization of titanium dioxide nanoparticles and their photocatalytic activity

In this study, we compared two low-temperature synthesis procedures for the large-scale production of titania nanoparticles (NPs). The first takes place in an aqueous medium with an acidic environment, by using a triblock polymer surfactant (Pluronic 123). The second involves a polycondensation reaction of alkoxide precursors at 70 °C in a water-in-oil (W/O) microemulsion with a volume ratio of 1:1, using cetylpyridinium bromide (CPB) as a cationic surfactant. The morphological and structural characterization of the samples was carried out through Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD). The photoactivity of the nanostructured titania was evaluated by measuring the photodegradation of Methylene Blue (MB). The solvent-free synthetic approach provided spherical titania nanoparticles mainly constituted by rutile crystallites with a very good synthetic yield. However, the photodegradation rate of MB for such titania nanoparticles ranges from 30 % to 40 %, after 1h under solar irradiation. Conversely, titania nanoparticles obtained through microemulsion synthesis show a photodegradation rate of more than 90 % comparable to titania P25. This high-yield synthesis leads to the formation of TiO2 nanoparticles characterized by small crystallite aggregates (rutile and anatase).

Influence of fly ash and granulated blast furnace slag on autogenous shrinkage and drying shrinkage of cement-based materials mixed with alkali accelerator and alkali-free accelerator

Shotcrete shrinkage and cracking are getting more threatening due to its high content of cementitious materials, small aggregate particle size and a large amount of accelerator, which poses a great threat to the construction safety of engineering structures. This paper studies the volume stability of cement mortar with different contents of fly ash (FA) and granulated blast furnace slag (GBFS) (10%/20%/30%) under the condition that the dosage of alkali liquid accelerator is 4% and that of the alkali-free liquid accelerator is 7%. This work is designed to provide a guidance for the basic research and practical application. By comparing the autogenous shrinkage, drying shrinkage, mass loss caused by drying condition, and internal humidity change of drying condition of cement mortar within 180 days of age. Combined with MIP analysis, XRD hydration product analysis, and SEM hydration product morphology analysis, the results show that both fly ash and granulated blast furnace slag can reduce the autogenous shrinkage and drying shrinkage of cement mortar mixed with accelerator to a certain extent, and the shrinkage reduction effect of FA is more prominent. The reduction effect of GBFS is more obvious at high dosages. Adding FA and high content of GBFS can increase the mass loss of cement mortar under drying shrinkage conditions. The high content of mineral admixtures will exacerbate the rate of decrease in the internal humidity of the cement mortar. The reasons for the reduction of autogenous shrinkage and drying shrinkage of cement mortar mixed with accelerators due to the addition of FA and GBFS are: the early activity of the two mineral admixtures is low, and the hydration process is slow. And the reduction of pore size with the range of 5-50nm leads to the reduction of capillary tensile stress and the weakening of the driving force for shrinkage deformation. Among them, the shrinkage reduction effect of fly ash is more pronounced. Therefore, mineral admixture can be used to replace a certain amount of cement in actual shotcrete construction, so as to increase the volume stability of shotcrete.

Coaggregation Occurs between a Piliated Unicellular Cyanobacterium, Thermosynechococcus, and a Filamentous Bacterium, Chloroflexus aggregans.

Cyanobacteria are widely distributed in natural environments including geothermal areas. A unicellular cyanobacterium, Thermosynechococcus, in a deeply branching lineage, develops thick microbial mats with other bacteria, such as filamentous anoxygenic photosynthetic bacteria in the genus Chloroflexus, in slightly alkaline hot-spring water at ~55 °C. However, Thermosynechococcus strains do not form cell aggregates under axenic conditions, and the cells are dispersed well in the culture. In this study, Thermosynechococcus sp. NK55a and Chloroflexus aggregans NBF, isolated from Nakabusa Hot Springs (Nagano, Japan), were mixed in an inorganic medium and incubated at 50 °C under incandescent light. Small cell aggregates were detected after 4 h incubation, the size of cell aggregates increased, and densely packed cell aggregates (100-200 µm in diameter) developed. Scanning electron microscopy analysis of cell aggregates found that C. aggregans filaments were connected with Thermosynechococcus sp. cells via pili-like fibers. Co-cultivation of C. aggregans with a pili-less mutant of Thermosynechococcus sp. did not form tight cell aggregates. Cell aggregate formation was observed under illumination with 740 nm LED, which was utilized only by C. aggregans. These results suggested that Chloroflexus filaments gather together via gliding motility, and piliated cyanobacterial cells cross-link filamentous cells to form densely packed cell aggregates.

Effects of Terracing on Soil Aggregate Stability and Erodibility in Sloped Farmland in Black Soil (Mollisols) Region of China

Soil aggregates are important indicators of soil structure stability and quality. The black soil region of northeast China, known for its high agricultural productivity, faces significant challenges due to soil erosion. This study investigates the impact of terracing on the stability and erodibility characteristics of soil aggregates in sloped farmlands, which is crucial for this important agricultural area. Three research sites with the same basic management modes were selected along a latitudinal gradient, from the mid-temperate zone to the cold temperate zone, in the black soil region of northeast China. The Savinov method was used to analyze the differences in soil aggregate size distribution, stability characteristics, and soil erodibility between terraced and non-terraced slopes at each research site. The results showed that terracing increased the content of large soil aggregates (&gt;0.25 mm) by 5.38–6.35%, with the increase becoming more pronounced from north to south. The improvement in soil structure varied by location and slope position, with the most significant improvement at the middle slope position. Terracing enhanced soil aggregate stability, reduced soil erodibility, and improved soil structure by increasing clay and soil organic matter (SOM) content and reducing soil bulk density (BD), promoting the conversion of small aggregates to large aggregates. Soil stability indicators such as water-stable aggregates (WSAs), mean weight diameter (MWD), and geometric mean diameter (GMD) were dominated by aggregates &gt; 5 mm, while erodibility indicators such as fractal dimensions (Ds) and the soil erodibility factor (K values) were mainly influenced by aggregates &lt; 0.25 mm. Terraces can improve the soil structure and stability of sloping farmland by increasing the content of large soil aggregates and enhancing overall soil quality. The benefits of these improvements increase with latitude. These findings provide critical insights for determining effective management practices for sloped farmlands in the black soil region under various site conditions. They offer scientific evidence for preventing soil erosion and improving soil quality, thus supporting the sustainable development strategy for protecting black soil and ensuring long-term agricultural productivity.

Decomposer faecal food web and C sequestration in soil. Can near infrared spectroscopy describe transfers and transformations from fresh organic inputs to protected forms in soil aggregates?

This study sought to characterize the transfer of agricultural organic inputs by macrofauna to the biogenic aggregate compartments of the soil and test the existence of a faecal food web process based on coprophagic feeding behaviours. In an experimental plantain crop field of Colombia, we applied the agroecological FBO (Fertilisation Bio Organique ®) technology, a nucleation technique which consists in planting perennial plants in 1.0 x 0.4 × 0.4 m deep trenches where low- and high-quality organic materials are added in a specific design, and endogeic earthworms are inoculated. We evaluated the effectiveness of Near Infrared Spectroscopy (NIRS) to track this flow of organic matter through different natural stages of decomposition represented by soil macroaggregates produced by soil macroinvertebrates as faecal pellets and casts. After one year, great part of the organic inputs had been either mineralized or incorporated into macroaggregates of different sizes and origins. Using NIRS and physicochemical laboratory analyses, we assessed the quality and quantity of organic matter in the different types of aggregates separated manually according to their origin: large biogenic aggregates (LBA), medium sized biogenic aggregates (MBA) and small biogenic aggregates (SBA); physical aggregates (PA), root aggregates (RA) and residual soil (RS).Earlier studies had shown that these structures were presumably formed by the activity of macroinvertebrates of three different functional groups, Diplopoda and Isopoda litter transformers for SBA, epidendogeic earthworms for MBA and mesohumic endogeic earthworms for LBA, organized in a feeding succession.A Coinertia analysis between showed significant covariation among the two sets of physico chemical (22 variables) and NIR spectral (100 different wavelength absorbances) characterization of 135 samples representative of the three classes of biogenic macroaggregates, physical aggregates and residual non macroaggregated soil, (RV = 0.55; p < 0.001). This analysis clearly separated biogenic structures and ranked them according to their size, from small SBA to medium sized MBA and large LBA. Physical PA aggregates and RS residual soil were projected close to the LBAs in the coinertia factorial plan. Multiple combinatorial data analysis CDA, associated 5 specific wavelength absorbance ranges with aggregate types and residual soil. Along the sequence from small to large biogenic aggregates, residual soil and physical aggregates, wavelengths associated to easily decomposed substrates (in ranges 1708–1716 nm; 1796–1948 and 2164–2316 nm) had progressively decreasing absorbances. Substrates associated to slowly decomposing aromatic, alkane and phenolic substrates either increased (1420–1436) or decreased (1284–1380) along this sequence.These results are compatible with the hypothesis of a progressive transformation and transfer of organic residues first into small biogenic aggregates that are presumably faecal pellets of Isopoda and Diplopoda, then to medium sized biogenic aggregates identified as casts of epiendogeic earthworms and finally to large biogenic aggregates expected to be the casts of the large endogeic earthworms present in these soils. A definitive verification of the hypothesis and the involvement of specific macroinvertebrates will require direct experimentation in controlled laboratory conditions.

Green Synthesis of SnO2 Nanocrystals Using Garcinia Mangostana L Fruit Peels Extract as Natural Capping Agent

The hydrothermal synthesis of SnO2 nanocrystals at a relatively low-temperature range of 95-100 °C was successfully conducted utilising Garcinia Mangostana L fruit peel extract as a natural capping agent. Characterisation of the synthesised SnO2 nanocrystals was performed using an X-ray diffractometer (XRD) for phase analysis and determination of crystallite size and a Scanning Electron Microscope (SEM) for morphology analysis. XRD analysis revealed the formation of phase-pure SnO2 nanocrystals, with distinct peaks at angles (2θ) of 26.01°, 33.89°, and 51.70° corresponding to miller indices (110), (101), and (211) as per JSPDS standard data. The absence of impurity peaks in the XRD pattern indicated the high purity of the synthesised SnO2 nanocrystals. SEM images exhibited differences in the size and morphology of the synthesised SnO2 nanocrystals with and without the extract. Specifically, the presence of the fruit peel extract led to a reduction in aggregate formation and inhibited crystal growth, resulting in smaller aggregates. These findings highlight the significant impact of Garcinia Mangostana L fruit peel extract on the hydrothermal synthesis of SnO2 nanocrystals with varied sizes and morphologies.

A novel multi-loss dynamic fusion-enhanced image segmentation model for welding spatter measurement

Quantitative detection of welding spatter is not only critical for evaluating and optimizing welding energy consumption, but also significant for improving welding quality. Inspired by the welder's visual estimation of the amount of spatter, a novel welding spatter measurement method based on image segmentation is proposed. Considering the challenges of welding spatter such as small object, aggregation, and unclear boundary, four loss functions, namely, Focal loss, Dice loss, Boundary loss, and Count loss, are designed to achieve global optimization for complex welding spatter. Furthermore, for the different spatter characteristics with different optimization difficulties, a multi-loss dynamic fusion method with differential optimization capability is designed. Finally, a welding spatter segmentation dataset is established and a comprehensive ablation and comparison test of the proposed methodology is carried out. The results indicate that the proposed method has an average F1-score metric of 83.52 % and a mean intersection over union metric of 75.11 %. These findings suggest the potential for vision-based quantitative estimation of welding spatter.

A Cationic Zn-Phthalocyanine Turns Alzheimer's Amyloid β Aggregates into Non-Toxic Oligomers and Inhibits Neurotoxicity in Culture.

Amyloid β peptide (Aβ) aggregation and deposition are considered the main causes of Alzheimer's disease. In a previous study, we demonstrated that anionic Zn-phthalocyanine (ZnPc) can interact with the Aβ peptide and inhibit the fibril-formation process. However, due to the inability of anionic ZnPc to cross the intact blood-brain barrier, we decided to explore the interaction of cationic methylated Zn-phthalocyanine (cZnPc) with the peptide. Using a ThT fluorescence assay, we observed that cZnPc dose-dependently and time-dependently inhibited Aβ1-42 fibril levels under in vitro fibril-formation conditions. Electron microscopy revealed that it caused Aβ1-42 peptides to form small aggregates. Western blotting and dot immunoblot oligomer experiments demonstrated that cZnPc increased rather than decreased the levels of oligomers from the very early stages of incubation. A binding assay confirmed that cZnPc could bind with the peptide. Docking simulations indicated that the oligomer species of Aβ1-42 had a higher ability to interact with cZnPc. ANS fluorescence assay results indicated that cZnPc did not affect the hydrophobicity of the peptide. However, cZnPc significantly increased intrinsic tyrosine fluorescence of the peptide after 8 h of incubation in fibril-formation conditions. Importantly, cell culture experiments demonstrated that cZnPc did not exhibit any toxicity up to a concentration of 10 µM. Instead, it protected a neuronal cell line from Aβ1-42-induced toxicity. Thus, our results suggest that cZnPc can affect the aggregation process of Aβ1-42, rendering it non-toxic, which could be crucial for the therapy of Alzheimer's disease.

Physical property responses of soils subjected to different degrees of erosion and seasonal freeze-thaw cycles in Northeast China

Changes in soil pores and aggregate stability due to freeze-thaw cycles (FTCs) are important causes of increased soil erosion during snowmelt. Soil erosion causes spatial redistribution of soils, enhancing soil heterogeneity and potentially impacting soil responses to FTCs. Nonetheless, there is minimal knowledge of the responses of soils subjected to different degrees of erosion to seasonal FTCs. To reveal the impact of seasonal FTCs, the dynamic variations of pore characteristics and aggregates of soils with four different degrees of erosion (original, degraded, deposited and parent soil) were measured, and the connections between influencing factors and soil properties were analyzed. The results showed that FTCs altered the structure of the soils and weakened their resistance to erosion and that soils with different degrees of erosion responded differently to FTCs. After seasonal FTCs, soil porosity increased (0.4 %-11.9 %) to some extent in all soils, with greater changes observed in the more eroded soils. Notably, capillary porosity exhibited a complex changing trend compared to total porosity. Degraded and parent soils showed a stable bulk density, while the original soil showed a decrease (2.1 %) in bulk density and the deposited soil showed an increase (18.4 %) in bulk density. With the increase of FTCs, the field capacity of original, degraded, and deposited soils exhibited a gradual decrease (15.1 %-18.5 %), while that of the parent soil slightly increased (0.9 %). After seasonal FTCs, the saturated hydraulic conductivity decreased for original and deposited soils (19.5 %-41.5 %), while it increased for degraded and parent soils (29.2 %-41.6 %). Throughout the FTCs, the proportion of the large aggregates decreased and the small aggregates increased, and the transformation was greater on the less eroded soils. The mean weight diameter and geometric mean diameter of the soils gradually decreased with increasing FTCs, while the change was smaller for the more eroded soils. After seasonal FTCs, the less eroded soils were at greater risk of erosion. Our results demonstrated that the number of FTCs had a more significant impact on soil physical properties compared to the temperature difference and soil water content. Overall, freeze-thaw action reinforced the spatial heterogeneity of soil properties, potentially intensifying soil erosion. These findings help reveal the effects of FTCs on the physical properties of soils with different degrees of erosion and deepen the understanding of the mechanism of FTCs on soil erosion processes.

Impact of non-reciprocal interactions on colloidal self-assembly with tunable anisotropy.

Non-reciprocal (NR) effective interactions violating Newton's third law occur in many biological systems, but can also be engineered in synthetic, colloidal systems. Recent research has shown that such NR interactions can have tremendous effects on the overall collective behavior and pattern formation, but can also influence aggregation processes on the particle scale. Here, we focus on the impact of non-reciprocity on the self-assembly of a colloidal system (originally passive) with anisotropic interactions whose character is tunable by external fields. In the absence of non-reciprocity, that is, under equilibrium conditions, the colloids form square-like and hexagonal aggregates with extremely long lifetimes yet no large-scale phase separation [Kogler et al., Soft Matter 11, 7356 (2015)], indicating kinetic trapping. Here, we study, based on Brownian dynamics simulations in 2D, an NR version of this model consisting of two species with reciprocal isotropic, but NR anisotropic interactions. We find that NR induces an effective propulsion of particle pairs and small aggregates ("active colloidal molecules") forming at the initial stages of self-assembly, an indication of the NR-induced non-equilibrium. The shape and stability of these initial clusters strongly depend on the degree of anisotropy. At longer times, we find, for weak NR interactions, large (even system-spanning) clusters where single particles can escape and enter at the boundaries, in stark contrast to the small rigid aggregates appearing at the same time in the passive case. In this sense, weak NR shortcuts the aggregation. Increasing the degree of NR (and thus, propulsion), we even observe large-scale phase separation if the interactions are weakly anisotropic. In contrast, systems with strong NR and anisotropy remain essentially disordered. Overall, the NR interactions are shown to destabilize the rigid aggregates interrupting self-assembly and phase separation in the passive case, thereby helping the system to overcome kinetic barriers.

Understanding the impact of soil components on the environmental existence of Nonylphenol:From the perspective of soil aggregates

Nonylphenol (4-NP) has significant adverse effects on the male reproductive system. 4-NP is commonly used in agriculture as a plasticizer and pesticide emulsifier. In the current study, two soil samples with different textures were collected to evaluate the impact of soil components on the environmental existence of 4-NP among soil aggregates. It was found that the presence of soil POM resulted in 4-NP exhibiting a significantly polarized distribution in soil aggregates, instead of the expected increase in content with decreasing particle size. High levels of organic matter and metal oxides result in a high carrying capacity of small aggregates for 4-NP in both soil textures, while POM results in a higher carrying capacity of large aggregates for 4-NP in clay soil. Another important finding is that the existence of 4-NP in soil was regulated by the percentage of aggregates. The results of contribution shown that although small aggregates in sand presented stronger 4-NP carrying capacity, whereas 4-NP was mainly distributed in large aggregates in sand. For clay soil, 4-NP was predominantly located in small aggregates with the 4-NP contributions of small aggregates amounting to 63.17%, despite the highest carrying capacity of 4-NP was observed in large aggregates. These results provide a theoretical basis to investigate the transport and transformation of 4-NP in the soil environment.

How nitrate and ammonium impact soil organic carbon transformation with reference to aggregate size

While nitrogen (N) deposition and over-fertilization enrich N in soil, it is unclear how it impacts soil organic carbon (SOC) transformation at the aggregate scale. Herein, a 90-day study reveals the transformation mechanisms of SOC in soil aggregates under nitrate and ammonium enrichment conditions. Results showed that nitrate treatment (NT) and ammonium treatment (AT) significantly increased SOC content by 15.6 % and 18.9 %, respectively. In addition, NT increased SOC accrual in large macro-aggregates (LMA), while AT increased SOC accrual in small macro-aggregates (SMA) and micro-aggregates (MA). Further analysis of pyrolysis products showed that N enrichment drove the transformation of labile soil organic matter (SOM) composition into recalcitrant SOM, with polysaccharides declining from 19–30 % to 2–13 %, while lipids rose from 18–27 % to 33–45 %. LMA and SMA contained more aromatic compounds than MA. This is linked to the inhibition of the expression of C degradation function genes, while almost all genes encoding SOC degradation are down-regulated under N enrichment. In the meantime, NT increased the abundance of genes encoding the degradation of N-containing compounds in LMA. Moreover, NO3− enrichment exerted a higher inhibitory effect on labile SOC degradation while NH4+ enrichment substantially inhibited recalcitrant SOC. Finally, Random Forest analysis confirmed that N enrichment elevated the importance of N-containing compounds' metabolism, which diminished when the size of soil aggregates decreased. In contrast, the importance of genes encoding saccharides and cellulose metabolism increased in smaller aggregates. This study highlights that both N type and aggregate size were determining factors in shaping SOC transformation in the N enrichment process.

Wheat gliadin/xanthan gum intermolecular complexes: Interaction mechanism and structural characterization

In this study, the interactions between wheat gliadin (GL) and xanthan gum (XG) were investigated to design new systems with potential applications as a gluten-free substitute product. Combining spectral with morphological and molecular docking methods allowed the establishment of the complexation mechanism between globular hydrophobic GL and the hydrophilic XG with an extended and partially disordered backbone. GL maintains intact its hydrophobic core even at high GL/XG ratios and organizes into small aggregate–type assemblies. The stable and uniform complexes have a low GL content, based on intermolecular hydrogen bonds and hydrophobic interactions. The GL/XG combining ratio influences the size, structure and interaction mechanism of the microparticles. The preferred sites of interaction and the binding affinities were determined by molecular docking on GL libraries and XG models. This research may provide significant knowledge for the development of low-GL wheat food products using a dietary fiber polysaccharide as a functional compound.

Dynamic interfacial adsorption and emulsifying performance of self-assembled coconut protein and fucoidan mixtures

The functional properties of protein are affected by their aggregation behavior and morphology. In this study, the self-assembled coconut protein aggregates with specific morphology, including small amorphous aggregates (WLA), spherical-like aggregates (SLA) and rod-like aggregates (RLA), were regulated to form. The self-assembled process resulted in a decrease in fluorescence intensity and an increase in the surface hydrophobicity of coconut protein. Fucoidan was added to improve the stability of protein solutions, and the interfacial adsorption behavior was evaluated by dilatational rheology analysis. The results showed that the aggregation state of coconut protein affected its ability to reduce surface tension, and the interfacial layers mainly exhibited elastic property at oil-water interface (tanφ < 0.5). For macroscale analysis, the emulsions based on self-assembled coconut protein exhibited smaller droplet size, better rheological properties and centrifugal stability, especially WLA and RLA. This study may provide a reference to inspire the utilization of self-assembled coconut protein in the food industry.