Slurry Treatment Research Articles

A comparison between in vitro and randomized in situ models for remineralization of artificial enamel lesions

The randomized study aimed to evaluate the comparability of in situ (iS) and in vitro (iV) study protocols regarding remineralization of artificial enamel lesions. Two toothpastes (group A: 1450 ppm sodium fluoride, group B: placebo 0 ppm F-), were investigated. IV, a pH-cycling model with toothpaste slurry treatment was applied for 10d. IS, remineralization was performed in 9 participants wearing splints with embedded enamel samples for 10 and 21d, randomly allocated to groups A and B. Samples were scanned by X-ray micro-computed tomography (μCT) and grayscale value line profiles corresponding to mineral density (rel.ΔZ) were analyzed. Statistical analyses were performed using MedCalc Statistical Software, v22.021. T-Test for dependent and independent data and analysis of variance (ANOVA) were used for further analyses (α = 0.05). Rel.ΔZ of fluoride treated samples (A) were iV = 40.2%, iS 10d = 11.5% and iS 21d = 46.1% (p > 0.05). Rel.ΔZ of placebo treated samples (B) were: iV = − 6.2%, iS 10d = 25.2% and iS 21d = 11.0% (p > 0.05). Remineralization potential of both toothpastes was significantly different regarding iV (p < 0.001) and iS after 21d (p = 0.034), while in case of iS 10d no significant difference was detected (p = 0.4). Despite different study protocols the μCT results after remineralization were comparable between iV and iS. The results suggest that selected studies can be carried out in faster, simplified iV studies using pH-cycling instead of iS studies.

Development of an efficient pretreatment process and multi-functional acetate co-metabolism strategy for the treatment of high strength biogas slurry using a newly isolated Chlorella sorokiniana

The efficient treatment of biogas slurry (BS) to meet the government’s discharge standards is a major challenge for most livestock and poultry enterprises. Although microalgae-based wastewater treatment has many significant benefits over traditional technologies, the poor ammonium tolerance of regular algal species, the pH imbalance during microalgae cultivation, and the complex and expensive BS pretreatment process greatly limit its application in BS treatment. Aimed at these issues, this study firstly isolated a robust Chlorella sp. with strong ammonium and acidic tolerance, as well as heterotrophy ability. To facilitate optimal pH stability and growth enhancement, a co-metabolism cultivation strategy based on the addition of 0.1 g·L-1 acetate in the form of fed-batch was developed. In addition, an efficient pretreatment process integrating ammonia reduction by air stripping with turbidity and chromaticity reduction by acidic precipitation was developed for the elimination of inhibitory factors of microalgal growth in BS. The isolated Chlorella sp. was capable of growing in the pretreated real BS and no growth inhibition was observed even in the undiluted BS. The acetate co-metabolism cultivation strategy obviously enhanced cell growth and pollutant removal and both NH4+ and total phosphorus were reduced to meet the discharge standards of China via 2.5-fold dilutions of BS. The accumulation of high-value biomass could not only fully cover the cost of wastewater treatment, but also bring significant returns. The net profit from treating 1 m3 of BS reached $ 116.2. This study provides a simple and efficient packaged process for the treatment of high strength BS and is expected to greatly reduce the process cost of BS.

Effect of Surface Treatment with Zirconium Dioxide Slurry on the Bond Strength of Resin Cement to Ultratranslucent Zirconia.

This laboratory study aimed to evaluate the effects of zirconium dioxide (ZrO2) slurry surface treatment on the bond strength of ultratranslucent zirconia to resin cement using different ceramic primers. The surface morphology was evaluated by scanning electron microscopy (SEM) and atomic force microscopy (AFM), and the interface was evaluated by SEM. Additionally, the phase composition was analyzed by X-ray diffraction (XRD). Specimens of zirconia (n=120) were obtained and divided into two groups according to the surface treatment: (1) airborne particle abrasion with 50-μm aluminum oxide (n=60) and (2) ZrO2 slurry (n=60). The 60 specimens were then further divided into three groups (n=20) according to the ceramic primer application: no primer (NP), Monobond N (MB), and Clearfil ceramic primer (CP). Four resin cement cylinders were built on each ceramic specimen. Half of the specimens (n=10) were subjected to a microshear bond strength (μSBS) test after 24 hours of storage in distilled water, and the other half (n=10) were subjected to a μSBS test after thermocycling. Additional specimens were prepared for SEM, AFM, and XRD analyses. According to the Kruskal-Wallis and Student-Newman-Keuls post hoc tests, the μSBS values were significantly higher for MB and CP than for NP (p<0.05), and there were no significant differences in μSBS for both surface treatments associated with MB and CP after 24 hours of storage (p>0.05). Thermocycling significantly decreased the μSBS values for all specimens, especially for the NP groups and ZrO2 slurry treatment groups, and gaps at the interface were observed by SEM. SEM and AFM analyses showed agglomerate-type irregularities on the ceramic surface for ZrO2 slurry treatment. XRD spectra showed that ZrO2 slurry did not cause phase transformation. It was concluded that ZrO2 slurry promoted irregularities on the ultratranslucent zirconia surface, not causing phase transformation; moreover, the values of μSBS were comparable to those of airborne particle abrasion with aluminum oxide. However, neither surface treatment nor ceramic primer prevented the degradation of the interface.

Enhancing concrete durability and strength: An innovative approach integrating abrasion and cement slurry treatment for recycled coarse aggregates

AbstractThis study introduces a novel approach to enhance the durability and strength of concrete by integrating abrasion and cement slurry treatments on surface‐treated recycled coarse aggregates (STRCA). Initial abrasion treatment removes aged mortar from the recycled coarse aggregate (RCA) surface, while subsequent cement slurry modification provides a fresh surface, reinforcing the bond with the concrete matrix. Through detailed material analyses including x‐ray diffraction, scanning electron microscopy, and energy‐dispersive x‐ray, microstructural changes are evaluated alongside compressive strength, drying shrinkage, electrical resistivity, and chloride ion penetration of STRCA‐based concrete. Results indicate significant improvements in mechanical properties and durability as the treatments eliminate mortar and enhance the interfacial connection with cement paste. Notably, a substantial 29.37% strength enhancement is observed at 25% replacement (STRCA 25), demonstrating treatment efficacy. While a reduction of 11.58% occurs at 100% replacement (STRCA 100), up to 75% replacement shows minimal strength loss, with optimal enhancements at 50% replacement. Additionally, STRCA concrete exhibits lower drying shrinkage (11%–20% reduction) and increased electrical resistivity (28%–36% rise), indicating improved durability at 100% replacement. Enhanced resistance to chloride ion penetration is also evident, with a 22%–28% increase at 100% replacement compared to conventional RCA. Overall, this study highlights the effectiveness of dual treatments for enhancing concrete properties using STRCA.

Effects of a new synthetic Fe-PAM flocculants on filtration and consolidation of bentonite slurry

To improve the dewatering performances of the waste slurry, a new Fe-Polyacrylamide (Fe-PAM) flocculant was synthesized. Comparative analysis with various ionic forms of polyacrylamide (PAM) reveals that the Fe-PAM flocculant has the shortest flocculation time, the lowest optimal shear gradient for flocculation, and the best filtration performance. The capillary suction time (CST) of Fe-PAM treated slurry was improved from 262.3 s (raw sample) to 6.0 s. Moreover, sludge treated with Fe-PAM exhibits the lowest porosity under the same consolidation pressure and the highest permeability at the same void ratio. In addition, mercury intrusion porosimetry (MIP) results indicated that the Fe-PAM-treated sample features the highest number of largesized inter-aggregate pores (average value of 184.2 nm and median value of 23.2 nm) and the greatest pore connectivity (60.55 %), contributing to its superior permeability. The effectiveness of Fe-PAM is further elaborated through a synergistic flocculation mechanism, encompassing the electrical neutralization effect of the inorganic metal Fe3+ and the adsorption bridging effect of PAM's long-chains. This study demonstrates the potential of the synthesized Fe-PAM flocculant to enhance the dewatering efficiency of high-water content slurry and offers valuable insights for synthesizing advanced flocculants in slurry treatment engineering.

15N mass balance technique for measuring ammonia losses from soil surface‐applied slurries containing various additives

AbstractBackgroundAnthropogenic ammonia emissions, primarily derived from agriculture, lead to air pollution, soil acidification, and surface water eutrophication, all of which adversely affect human health and ecosystems. Slurry treatment technologies in the form of additives represent an underutilized means of reducing gaseous emissions. Information regarding the potential of additives to reduce ammonia in soil surface‐applied slurries is scarce.AimThis study aims to develop a 15N mass balance technique to quantitatively measure ammonia losses from different slurries containing multiple additives that are applied to outdoor soil‐filled containers.MethodsThe experiments were performed under free‐air conditions. Isotopically labeled slurries from biogas, cattle, and pigs containing 18 additives were surface‐applied to soil‐filled containers and exposed for 72 or 48 h. The additives included inorganic and organic adsorbents, five amounts of sulfuric acids, molasses ± effective microorganisms, and water dilution. After termination of the ammonia loss period, a suite of soil preparation steps for the quantitative recovery of the labeled ammonium remaining in the soil was developed, and subsequently the loss of NH4‐N was determined.ResultsIn the control treatments, ammonia losses from biogas, cattle slurries, and pig slurries averaged 54.4%, 33.9%, and 11.0%, respectively. The adsorbents did not decrease or only slightly decreased ammonia emissions. Ammonia abatement by sulfuric acid was nearly complete at pH values of 5.9 and 5.8 for the biogas and pig slurry, respectively, and about 80% at pH 5.2 for the cattle slurry. In comparison, more moderately decreased pH values with sulfuric acid showed a similar reduction as molasses and a 1:1 dilution for the three slurries. Adding microorganisms to the molasses did not further decrease ammonia losses.ConclusionThe newly developed 15N mass balance technique, which allows a precise estimate of ammonia losses, can serve as a reference method to assess ammonia losses from field‐applied slurries containing various additives and as a standard comparison technique for other ammonia measurement techniques.

Yeast Expressing a Phage Endolysin Reduces Endogenous Clostridium perfringens Ex Vivo in 21-Day-Old Broiler Chicken Intestinal Fluids.

The phage endolysin PlyCP41 when purified from Escherichia coli exhibits lytic activity against Clostridium perfringens (CP) in vitro. The anti-clostridial activity of PlyCP41 endolysin expressed in transgenic yeast (Saccharomyces cerevisiae) was verified in phosphate buffered saline via mixing experiments with cultured CP and transgenic yeast slurries followed by serial dilution plating and colony counts on tryptose sulfite cycloserine (CP indicator) plates. The transgenic yeast containing PlyCP41 resulted in a log10 4.5 reduction (99.997%; P < 0.01) of the cultured CP. In addition, this serial dilution plating assay was used to demonstrate that transgenic yeast slurries could reduce the endogenous CP content in fluids from three different gastrointestinal regions (proximal, medial, and distal) from 21-day-old broiler chickens. The transgenic yeast treatment of gut slurries resulted in a log 10 1.19, 4.53, and 1.28 reduction in proximal, medial, and distal gut slurries (90% to 99.99% of the endogenous CP; P < 0.01), respectively, compared to nontreatment controls. These results indicate that the phage endolysin PlyCP41 expressed in S. cerevisiae is effective at reducing the endogenous CP in gastrointestinal fluids of broiler chickens. Future studies will measure the anti-CP effect in vivo by administering transgenic yeast to broiler chickens in the feed.

Mechanism of geopolymeric solidification in shield-tunnelling slurry from diverse sources: The role of bentonite adsorption in influencing the reaction process

Geopolymeric solidification is an effective process for treating engineering waste slurry, yet its efficacy varies with source of slurry. This study examined four shield tunneling spoil types for geopolymeric solidification. The unconfined compressive strength of the best sample was 17–126% higher than the least effective one. A significant negative correlation between strength and bentonite indices was found. Subsequently, the addition of bentonite to the shield tunneling spoil for experiments, followed by various analyses, comfirmed bentonite's negative impact on strength. Bentonite forms agglomerates during geopolymerization, adsorbing hydration gel, weakening the direct linkage of the hydration gel; they also adsorb elements such as Al, Ca, and Na, hindering the formation of geopolymer products. However, the final adsorption ratio was not within the optimal range for geopolymer reactions, impeding the improvement in curing strength. This study offers the theoretical guidance for improving engineering waste slurry treatment processes.

Challenges When Assessing Water-Related Environmental Impacts of Livestock Farming: A Case Study of a Cow Milk Production System in Catalonia

Water availability is a local issue of growing importance in Mediterranean areas where water scarcity linked to climate change and population growth is already leading to increased competition for this resource. This study is aimed at the following: (i) assessing the water-related environmental impacts (water use, freshwater ecotoxicity and eutrophication, marine eutrophication, acidification, human toxicity, and ionizing radiation) along the production chain of cow milk in Catalonia, northeastern Spain; and (ii) addressing the issues encountered (modelling choices, data gaps and inconsistencies) which t can affect the quality of results when performing a water-footprint comprehensive assessment, focusing on water use and associated water scarcity impacts. The scope included the process from the extraction of raw materials up to the distribution of the packaged fat- and protein-corrected milk to the distribution centres of the supermarket chains and markets. Results showed the farm stage to be determinant (contributing to over 60% of the impact), due to the impact of feed production. Impact results were in the range of the European benchmark given by the Product Environmental Footprint Category Rules for dairy products, except for the water scarcity footprint which was one order of magnitude larger than the reference value, due to water scarcity in Spain. Considering compound feed ingredients with a lower water scarcity footprint, and research into slurry treatment for its use as irrigation and cleaning water (without compromising safety and health) could help reduce this impact. Water accounting and traceability along the production chain could support the dairy industry to take responsibility for the consequences of their production choices.

Effect of cavitation treatment of coal-water slurries on a droplet average size in a jet

Relevance. Transition to environmentally friendly energy technologies, due to modern requirements for environmental protection, involves the search and creation of new energy sources, including fuels. One of the ways to meet these requirements and maintain the same level of energy production by thermal power plants is the transition to multicomponent fuels. The most promising and affordable boiler fuels from the point of view of energy, ecology and economy are coal-water slurries. In this regard, the study of the properties and characteristics of such fuels is relevant in many countries. Aim. Experimental studies of the effect of the coal-water fuel cavitation treatment duration on a droplet average size in a jet after spraying with a pneumatic nozzle and substantiation of the efficiency of such approach for practical application. Object. Experimental studies were carried out with coal-water slurries based on long-flame coal (D grade) with addition of 10 and 20 wt % by weight of pyrogenetic liquid. A slurry, consisting of just coal and water, without the addition of pyrogenetic liquid, was used as a reference sample. Method. The coal-water slurries were prepared in a rotary hydrodynamic cavitation generator. A pneumatic nozzle with external mixing was used to spray the coal-water slurries. The average size of fuel droplets after spraying was determined using the Interferometric Particle Imaging method. Results. Experiments on the preparation of coal-water slurries with pyrogenetic liquid showed an increase in the dynamic viscosity of the fuel. An increase in the duration of treatment of the slurries in a rotary hydrodynamic cavitation generator reduced the viscosity by 54%. The average size of droplets in the jet was reduced by 22%.

In-situ methane enrichment in anaerobic digestion of food waste slurry by nano zero-valent iron: Long-term performance and microbial community succession

In this work, nano zero-valent iron (nZVI) was added to a lab-scale continuous stirring tank reactor (CSTR) for food waste slurry treatment, and the effect of dosing rate and dosage of nZVI were attempted to be changed. The results showed that anaerobic digestion (AD) efficiency and biomethanation stability were optimum under the daily dosing and dosage of 0.48 g/gTCOD. The average daily methane (CH4) yield reached 495.38 mL/gTCOD, which was 43.65% higher than that at control stage, and the maximum CH4 content reached 95%. However, under single dosing rate conditions, high nZVI concentrations caused microbial cell rupture and loosely bound extracellular polymeric substances (LB-EPS) precipitation degradation. The daily dosing rate promoted the hydrogenotrophic methanogenesis pathway, and the activity of coenzyme F420 increased by 400.29%. The microbial analysis indicated that daily addition of nZVI could promote the growth of acid-producing bacteria (Firmicutes and Bacteroidetes) and methanogens (Methanothrix).

Renovation of grasslands with grass and white clover – Effects on yield and carbon sequestration

There is a pressing need to support farmers' decisions on grassland renovation, based on sound scientific evidence regarding its effects on productivity, herbage quality and soil organic carbon stocks. To quantify these effects a long-term experiment with grass/white clover swards was set up at the Lindhof research farm in Northern Germany in 1995. Treatments included control plots of undisturbed grassland as well as 10 grassland renovations starting after 10 (2005) years and repeated on different plots 10 times until 2019, and without and with addition of slurry (equivalent to 240 kg N ha−1 yr−1). Grassland renovation resulted in a significant drop in biomass production in the first year after renovation, and the slightly higher yields in the third year after renovation could not compensate for this drop. Yields from the year of renovation to three years afterwards were generally lower, with average reductions over the 4-year periods of 2600 kg DM ha−1 for the treatments without slurry and 1500 kg DM ha−1 for the slurry treatments. Differences in herbage quality between permanent and renovated grassland were negligible and generally not statistically significant. The soil organic carbon showed a rapid and significant drop in the year of renovation, followed by a gradual increase. Without slurry application, the initial levels of soil organic carbon stocks could not be reached even after a period of 18 years following renovation, and with slurry application, it took about 8–10 years. Deep ploughing to a depth of 30 cm did not increase the SOC stocks compared with the undisturbed permanent grasslands, suggesting that the topsoil has not reached the carbon saturation level. We conclude that maintaining productivity of permanent grassland without renovation measures is a promising way towards yield stability and natural climate solutions.

Experimental study on dredged slurry treatment with straw as horizonal drains by vacuum preloading

This paper presents a novel method of treating high water content dredged slurry by vacuum pre-pressure using woven rice straw as horizontal drainage boards (SHDs). This not only helps to avoid the impact of plastic drainage boards on the environment, but also helps to utilize agricultural related waste. The study aimed to investigate the effect of SHDs degradation on the vacuum transfer within the horizontal drainage boards and the consolidation characteristics of dredged slurry. Indoor vacuum preloading model tests were conducted to investigate soil settlement, straw degradation, vacuum variation and water content distribution within the drainage board. The results show that SHDs can significantly improve the consolidation of dredged slurry. The closer the straw is to the interface between the drainage board and the vacuum pipe, the greater the degree of degradation. The water content was distributed with depth in an oblique “z” shape, while a point with higher water content existed at 1/3 of the soil above the drainage boards. In addition, the low water content zone around the drainage board shows an elliptical shape due to the influence of gravity. These research results have some reference value for the ecological treatment of dredged slurry and straw.

Experimental study on environmental chemical effect and purification treatment of waste slurry in horizontal directional drilling

The waste slurry generated during Horizontal Directional Drilling (HDD) has detrimental effects on water sources, soil, and organisms. Existing studies lack quantitative analysis and assessment of the environmental hazards of waste slurry. The treatment of waste slurry is usually done by physical curing or chemical curing agents. The treatment effect of these treatments is unsatisfactory or not environmentally friendly enough. Research on the application of Microbially Induced Calcite Precipitation (MICP) in waste slurry purification can help provide innovative solutions. This study began by acquiring the basic properties, mineral composition, and particle size distribution of the waste slurry through experiments. The effects of different concentrations of waste slurry on soil chemical properties were compared, including the measurement of total dissolved solids (TDS), turbidity, and heavy metal ion concentrations of waste slurry supernatant. Additionally, the environmental impact of the waste slurry was assessed from an electrochemical, turbidity, and ionic composition perspectively. A combination formulation containing 2.8 wt% urease, 5 wt% urea, and 3.5 wt% calcium chloride was used to purify the waste slurry. The supernatant of the MICP treated waste slurry undergoes electrochemical testing to evaluate the effectiveness of MICP technology for slurry treatment. The findings indicate that both chemical additives and stratum contaminants significantly contribute to slurry pollution. When the waste slurry constitutes more than 40 wt% of the soil mass fraction, the electrical conductivity (EC) of the soil surpasses the threshold of 1000 μs/cm, and the pH level rises to approximately 9.5, rendering it unsuitable for plant growth. Moreover, the TDS, turbidity, and heavy metal ion concentrations in the waste slurry supernatant exceed the permissible environmental discharge limits. Notably, the concentration of arsenic (As) exceeds the national standard by 18 times, cadmium (Cd) by 20 times, and selenium (Se) by 208 times, posing a high risk of environmental pollution. However, the comparison of ion concentrations in the supernatant before and after MICP treatment reveals that MICP demonstrates effective cementation and co-precipitation of heavy metal ions. MICP purified 66.6 % of arsenic (As), all cadmium (Cd), 75 % of phosphorus (P), and 76 % of selenium (Se) from the waste slurry supernatant. The results of this research contribute to the quantitative evaluation of the environmental hazards of waste slurry. It also helps to promote the application of MICP in waste slurry purification.

Effect of the strength grade of parent concrete on the performance of recycled aggregate treated by cement-fly ash slurry under prolonged soaking duration

The detrimental effects of quarrying natural resources have contributed to large-scale environmental pollution. Utilizing recycled concrete aggregate (RCA) obtained from construction and demolition waste as a replacement for natural aggregates has become an effective solution for environmental sustainability. However, the inevitably diminished characteristics of the adhered mortar and the existence of an additional interfacial transition zone (ITZ) have negatively suppressed the widespread use of these materials in production lines. The present study aims to comprehensively investigate the effects of a cement-fly ash slurry with a mass ratio of 50% of the total weight of RCA on the microstructure and engineering properties of RCA derived from three different parent concretes with strength grades of 20, 30, and 60 MPa. The RCAs were soaked for 24 h, 48 h, and 72 h to evaluate the long-term effects of the pozzolanic slurry. A sequence of laboratory tests was conducted, including mercury intrusion porosimetry (MIP) test, thermal gravimetric–differential thermal analysis (TG-DTA), X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses, and measurements of water absorption and crushing value. The MIP results demonstrated a significant increase in medium capillary and gel pores, and a reduction in large capillary pores in the RCA with an increase in soaking time. TG-DTA results indicated an increase in Ca(OH)2 content during the first 24 h and 48 h of treatment. However, after 72 h of treatment, the Ca(OH)2 content of the treated RCA decreased in all cases. XRD analysis revealed the appearance of a few additional ettringite peaks among the treated RCAs in all cases. SEM images demonstrated the presence of additional hydration products after treatment, which filled the pores and improved the surface texture. Furthermore, after treatment, the crushing value of all treated RCA decreased by 6.0–27.1%, whereas the water absorption decreased by 12.0–30.9% compared to the untreated RCA. Based on several experimental results, the formation of hydration products and the improvement mechanism of RCA from three different parent concretes by slurry treatment with different soaking durations were discussed. In conclusion, a prolonged impregnation time of 72 h yielded a dominant performance, effectively intensifying their inferior properties, regardless of the initial strength grade of parent concrete.

Evaluate the partial nitrification/anammox biofilm system treating the anaerobic biogas slurry of multisource organic solid waste

Anaerobic digestion used for treating organic solid waste (OSW) usually produces much biogas slurry with high ammonia levels and low C/N ratio, suitable for partial nitrification/anammox (PN/A) process. However, the multisource OSW makes the biogas slurry complex, which may affect the stability of PN/A system. Therefore, this study explored the performance of a PN/A biofilm system treating biogas slurry with different water qualities. Different biogas slurry slightly affected nitrogen removal efficiency of the system (maintained at 90 %), whereas the contribution of anammox decreased gradually. The organic compounds in the biogas slurry were complex and difficult to degrade, but changed the microbial structure of the system. Complex biogas slurry promoted extracellular polymeric substances (EPS) secretion, helped maintain the stability of the biofilm and reduce the impact of the external environment. The relative abundance of Candidatus_Kuenenia, which was the dominant anammox genus, decreased from 9.02 % to 1.26 %. In addition, the relative abundance of Denitratisoma and Thauera decreased sharply, however, that of Truepera increased from 0.07 % to 6.52 %, making it the dominant denitrifying bacterial genus in the system. This study provides a reference for application of PN/A systems in the treatment of actual biogas slurry.

Efficient production of single cell protein from biogas slurry using screened alkali-salt-tolerant Debaryomyces hansenii

Production of single cell protein (SCP) by recovering ammonia nitrogen from biogas slurry shows great potential against protein scarcity and unsustainable production of plant and animal proteins. Herein, a high-alkali-salt-tolerant yeast strain, Debaryomyces hansenii JL8-0, was isolated and demonstrated for high-efficient SCP production. This strain grew optimally at pH 8.50 and 2500 mg/L NH4+-N, and it could efficiently utilize acetate as the additional carbon source. Under optimal conditions, SCP biomass of 32.21 g/L and productivity of 0.32 g/L·h−1 were obtained in fed-batch fermentation. Remarkably, nearly complete (97.40 %) ammonia nitrogen from biogas slurry was recovered, probably due to its high affinity for NH4+-N. Altogether, this strain showed advantages in terms of cell biomass titer, productivity, and yield. A cultivation strategy was proposed by co-culturing D. hansenii with other compatible yeast strains to achieve high-efficient SCP production from biogas slurry, which could be a promising alternative technology for biogas slurry treatment.

Towards enhanced treatment of piggery biogas slurry in tidal flow constructed wetlands by regulation of hydraulic retention time

Livestock farming wastewater is complex in composition, containing a large number of pathogenic microorganisms and ammonia nitrogen (NH4+-N) with high concentration, which makes biological treatment difficult. In this study, tidal flow constructed wetlands were applied to treat piggery biogas slurry. Compared with a continuous flow constructed wetland, two tidal influent modes (hydraulic retention time: idling time for 12 h:12 h and 20 h:4 h, namely TFCW1 and TFCW2) were set up to explore treatment performances on chromaticity, turbidity, chemical oxygen demand (COD), total nitrogen (TN), NH4+-N and total phosphorus (TP), and determine the relationship between nitrogen transfer and dissolved oxygen (DO) contents. The fluorescence spectra of dissolved organic matter (DOM) in constructed wetlands were analyzed by excitation-emission matrix spectroscopy-parallel factor analysis to reveal its composition and content of humus. The results showed that tidal flow constructed wetlands obviously improved the removal of chromaticity and turbidity by 44.6% and 10.1%, in comparison to continuous flow constructed wetland. TFCW2 had enhanced reduction on pollutants, with removal efficiencies of COD, NH4+-N and TP at 78.6%, 82.1% and 84.0%, respectively. Furthermore, three fluorescent components were detected in TFCW2, and the value of Fmax was increased in the effluent, among which the Fmax of C3 (Humic-like substances) increased obviously from 0.320 to 9.39. The ratio of C2 (Aquatic fulvic acids) to C3 showed a slow increasing trend in the daily outflow of TFCW2, while C1 (dark-incubation substances) remained relatively constant. Fluorescence index (FI), humification index (HIX) and biological index (BIX) increased from 1.35 to 2.09, 0.210–1.51, and 0.240–1.05, respectively, showing that the effluent from the tidal flow constructed wetland had a high microbial activity. This study provided a new insight for the advanced treatment of livestock biogas slurry with a high NH4+-N content.

The Quality of Dairy Cow Bedding and the Occurrence of Environmental Mastitis: Review

An excessive incidence of inflammation of the mammary glands of dairy cows has been observed in many dairy farms in the past, which has been demonstrably correlated with an increased incidence of microbial pathogens in livestock environments. The causes of mastitis can be infectious or non-infectious in nature, one of the main sources of this disease is litter material in the lager. The issue of mastitis has a significant impact on the economic value of dairy cows and therefore it is necessary to create effective measures that eliminate the appearance of pathogenic microorganisms. In this respect, litter material obtained by physical separation and thermal treatment of slurry may represent a suitable alternative ensuring inhibition of the development of dangerous bacteria while maintaining the comfort of dairy cows. Current evidence on the safety and benefits of such material is limited and data on effects on clinical and/or subclinical mastitis are insufficient. In this review, therefore, we would like to shed light on the onset of mastitis in dairy cows and point out possible procedures for treating slurry in practice.

Short-Term Nitrous Oxide Emissions from Cattle Slurry for Silage Maize: Effects of Placement and the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Cattle slurry is an important nitrogen source for maize on dairy farms. Slurry injection is an effective measure to reduce ammonia emissions after field application, but with higher risk of nitrous oxide emission than surface application. This study compared soil mineral nitrogen dynamics and nitrous oxide emissions with two ways of application. First, traditional injection at 25 cm spacing between rows followed by ploughing (called “non-placed slurry”), and second, injection using a new so-called goosefoot slurry injector that placed the slurry in ploughed soil as a 30 cm broad band at 10 cm depth below maize crop rows with 75 cm spacing (named “placed slurry”). Furthermore, the effect of treating slurry with the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) in Vizura® was tested with both application methods. The field experiment was conducted on a sandy loam soil in a temperate climate. Both nitrous oxide emissions, and the dynamics of soil mineral nitrogen, were monitored for eight weeks after slurry application and seeding of maize using static chambers. The level of nitrous oxide emissions was higher with non-placed compared to placed slurry (p &lt; 0.01), mainly due to higher emissions during the first four weeks. This might be due to higher rates of nitrification and in turn stimulation of denitrification. In both placed and non-placed slurry treatments, Vizura® caused higher soil ammonium concentrations and lower nitrate concentrations (p &lt; 0.001), particularly from 3 to 8 weeks after slurry application. The final level of soil nitrate was similar with and without the nitrification inhibitor, but higher with placed compared to non-placed slurry. Adding Vizura® to non-placed slurry reduced nitrous oxide emissions by 70% when compared to untreated slurry. Surprisingly, there was a non-significant trend towards higher cumulative emissions from placed slurry with the nitrification inhibitor compared to untreated slurry, which was due to higher emissions in the last part of the monitoring period (5–7 weeks after slurry application). Possibly, degradation of the nitrification inhibitor and nitrification activity inside the slurry band as the soil dried promoted nitrous oxide emissions by this time. In summary, placement of untreated slurry in a broad band under maize seeds reduced nitrous oxide emissions compared to non-placed slurry with more soil contact. A comparable reduction was achieved by adding a nitrification inhibitor to non-placed slurry. The pattern of nitrous oxide emissions from placed slurry treated with the inhibitor was complex and requires more investigation. The emission of nitrous oxide was highest when nitrate accumulated in soil around decomposing cattle slurry, and mitigation strategies should aim to prevent this. This study demonstrated a potential for mitigation of nitrous oxide emission by placement of cattle slurry, which may be an alternative to the use of a nitrification inhibitor.