This study evaluates the effect of rubber crumb on the microstructural properties of concrete which was used to partially replace fine aggregate by 0, 4, 8, 12, and 16% proportion and designated as M0, M4, M8, M12, and M16 respectively. The wet and hardened properties of concrete were investigated by conducting a slump test and a compressive strength test, and by measuring the concrete bulk density. The crack formed as a result of compressive strength was investigated using a digital microscope, while the micro-cracks formed in the concrete structure were investigated using a scanning electron microscope (SEM). The results showed that concrete slump for all the samples range between 25 and 10 mm, with M0 and M4 having the highest slump. The compressive strength and density of the concrete decreased as the amount of rubber crumb increased in the mixture, with M0 having a compressive strength of 51.88 N/mm2 and M16 having the lowest compressive strength of 19.40 N/mm2. The SEM analysis showed that there was a weak bond between the concrete paste and the rubber crumb, which resulted in to a weak adhesive force, and the development of micro-cracks which contributed to the week compressive strength in concrete with higher rubber crumb.