Slow Maturation Research Articles

PRAKTIK FATHERLESS DAN KAITANNYA DENGAN PENGASUHAN ANAK DALAM FIKIH ISLAM DAN HUKUM PERKAWINAN DI INDONESIA

The phenomenon of fatherless, which is increasingly prevalent in Indonesia, has become a serious concern due to its negative impact on children’s development, both physically, mentally, and spiritually. This study aims to analyze the perspective of Islamic Fiqh and the Marriage Law regarding the role of fathers in child care, identify the father’s obligations in child care according to Islamic Fiqh and the Marriage Law, and determine the impact of fatherless practices on child growth. This study uses a library research method with a qualitative approach. The data used are primary data and secondary data obtained from various sources of literature, such as books, journals, and official documents. Data collection techniques were carried out through literature studies, and data analysis used content analysis. The results of the study show that Islamic Fiqh and the Marriage Law in Indonesia both emphasize the importance of the father’s role in child care. Fathers have an obligation to provide sustenance, educate and guide children, as well as provide affection and attention. However, fatherless practices in Indonesia are caused by several factors, such as high divorce rates, unbalanced parenting patterns, patriarchal culture, and the death of the father. The impact of fatherless on children’s growth and development can be in the form of father hunger, which is a condition of psychological damage that results in low self-esteem, difficulty adapting, slow psychological maturity, difficulty managing emotions, and lack of decision-making ability.

A Review of Grouper Fisheries Management in the Southeastern and Caribbean U.S.: Challenges, Successes, and Future Directions

Groupers (Epinephelidae) are ecologically important mesopredators that support valuable fisheries across the globe. Many groupers display slow growth and maturity, high longevity, ontogenetic habitat shifts, spawning-related migrations and aggregations, and protogynous hermaphroditism, which make them susceptible to overexploitation. In this review, I synthesize available information related to the management of grouper fisheries across the southeastern and Caribbean U.S. I highlight current management challenges, such as managing multispecies reef fish fisheries with growing recreational fishing effort. I discuss management interventions with limited success, such as establishing marine protected areas to improve the populations of groupers that display protogynous hermaphroditism. I also highlight management successes, such as recovering historically depleted grouper stocks, and ecosystem-based considerations in grouper stock assessments. I discuss how climate change and anthropogenic effects are expected to affect groupers. Lastly, I provide examples of stakeholder involvement in monitoring and management efforts directed at grouper stocks. The purposes of this review are to demonstrate the complexities of managing grouper fisheries and provide a road map for future research and conservation efforts into these economically and ecologically relevant fishes within and beyond the region.

Hubungan Status Gizi Dengan Perkembangan Anak Usia 4-5 Tahun di TK Aisyiyah Bustanul Atfal 2 Labuhan Ratu Baru Kecamatan Way Jepara

Nutrition is one of the aspects that affects the growth and development of children. Nutritional status is an important health indicator, and vulnerable groups facing nutritional issues include children under five years old. Signs of developmental disorders include slow maturation of nerve cells, delayed motor skills, decreased intelligence, sluggish social reactions, and difficulties in speaking, language, and independence. Nutritional problems in children according to weight-for-age (BB/U) include undernutrition, short stature (TB/U), and underweight (BB/TB). The purpose of this study is to determine the relationship between nutritional status and the development of children aged 4-5 years at Aisyiyah Bustanul Atfal 2 Kindergarten. The subjects of this study are all children aged 4-5 years enrolled at Aisyiyah Bustanul Atfal 2 Kindergarten in Labuhan Ratu Baru Way, Jepara District, totaling 54 children. This study uses total sampling technique. The methodology employed is quantitative research with a cross-sectional design, and data collection was conducted using a microtoise to assess height, a digital scale to measure weight, and the KPSP questionnaire to evaluate nutritional status in relation to child development. The study findings indicate a significant relationship between nutritional status according to weight-for-age (BB/U) and child development (p=0.000) with a strong negative correlation (r= -0.748). There is a significant correlation between nutritional status according to height-for-weight (BB/TB) and child development (p=0.000) with a strong positive correlation (r=0.610). There is also a significant relationship regarding the correlation between nutritional status according to height-for-age (TB/U) and child development (p=0.025) with a weak positive correlation (r=0.304). It is advisable to provide education and early detection of developmental issues in toddlers experiencing nutritional disorders.

Synergistic effects of mediated by different 1,2-epoxybutane addition numbers butoxylated alkyl block alcohol ethers and SDS in mixed systems

In this study, nonionic surfactant butoxylated alkyl block ether (AE9Bn) and anionic surfactant sodium dodecyl sulphate (SDS) with different BO additions were mixed at different molar ratios to make a hybrid system. The interaction parameters and aggregation behaviors of the blended systems were investigated by observing the average direct particle drop distribution and microscopic aggregates in combination with the results of static surface tension tests of the blended systems. The diffusion process of the mixed systems was investigated using dynamic surface tension tests. The dynamic contact angle and foam properties of the solutions of the mixed systems were also studied. Finally, based on the experimental results, it was concluded that the mixed system of AE9Bn (n=2,5) and SDS formed a synergistic effect that significantly led to a decrease in CMC, with an increase in the number of BO additions leading to a more significant decrease in CMC. The mixed system is a non-ideal mixed system, which is consistent with the kinetic mechanism of mixing and diffusion. The hybrid system has good aggregation pattern and wetting diffusion behavior and has low foam stability due to slow maturation of Ostwald.

Rev or restrain: Mechanisms of human-specific synaptic neoteny

In the current issues of Neuron and Cell Reports, Libé-Philippot etal.1 and Assendorp etal.2 identify interactions between human-specific SRGAP2C, synaptic regulator SRGAP2A, and neurodevelopmental disorder-associated proteins SYNGAP1 and CTNND2 that slow synaptic maturation in human neurons.

Evolution history and the importance of genomic diversity facing climate change. The case of Agave marmorata Roezl., a microendemic agave used for mezcal production

Social Impact StatementFor hundreds of years, Agave marmorata plants have been used in the production of alcoholic beverages in Mexico. This species is very important in small‐scale rural economies because it is a large plant, yielding five liters of mezcal. However, the production of these beverages takes place when it reaches its reproductive stage, which takes up to 35 years. Due to its slow maturation and high demand, it is considered an endangered species. Therefore, as a conservation strategy, this study proposes the creation of nurseries, genetic breeding programs, and demographic monitoring of wild populations to counteract the extraction of wild plants and, the conservation of the genetic diversity.Summary Agave marmorata Roezl., is an endemic species distributed in the states of Oaxaca and Puebla, Mexico, and locally is widely used to produce mezcal. We assessed the genomic diversity and differentiation using the RADseq method and 29,101 high‐quality single nucleotide polymorphisms (SNPs) in wild plants and grown under three different management types (cultivated, plants used as live fences, and young plants growing in nurseries). We examined the demographic history and used species distribution modeling to understand the future of A. marmorata under scenarios of climate change. We found high levels of genomic diversity (HS = 0.229) and moderate levels of inbreeding (FIS = 0.106 and Fhat3 = 0.190). The cultivated samples harbored less genetic diversity than the wild plants. Furthermore, we estimated low differentiation between cultivated and wild localities (FST = 0.037). In the wild samples, we identified two main genetic groups, one in the East and another in the West of its distribution area. This genetic structure possibly derived from a population contraction during the Pleistocene (~216,879.75 BP) and the formation of two refugia in small areas with climatic stability. Furthermore, the demographic reconstruction indicated that A. marmorata went through a recent population expansion event, with a large current Ne (Ne = 8,009). The future climate change models indicated contrasting possible changes in its distribution range, from an increase to the reduction of its suitable habitat, differences related to model parametrization, and future levels of CO2 production. We propose conservation measures for the different management types of the species while also considering the biotic and abiotic interactions of Agave marmorata.

Phenotypic plasticity in the sailfin molly III: Geographic variation in reaction norms of growth and maturation to temperature and salinity.

Phenotypic plasticity, the ability of a single genotype to produce different phenotypes under different environmental conditions, plays a profound role in several areas of evolutionary biology. One important role is as an adaptation to a variable environment. While plasticity is extremely well documented in response to many environmental factors, there is controversy over how much of that plasticity is adaptive. Evidence is also mixed over how often conspecific populations display qualitative differences in the nature of plasticity. We present data on the reaction norms of growth and maturation to variation in temperature and salinity in male and female sailfin mollies (Poecilia latipinna) from three locally adjacent populations from South Carolina (SC). We compare these reaction norms to those previously reported in locally adjacent populations from north Florida (NF). In general, patterns of plasticity in fish from SC were similar to those in fish from NF. The magnitude of plasticity differed; fish from SC displayed less plasticity than fish from NF. This was because SC fish grew faster and matured earlier at the lower temperatures and salinities compared to NF fish. This is a countergradient pattern of variation, in which SC fish grew faster and matured earlier in conditions that would otherwise slow growth and delay maturity. Among fish from both regions, males were much less plastic than females, especially for length at maturity. While there was no detectable heterogeneity among populations from NF, males from one of the SC populations, which is furthest from the other two, displayed a qualitatively different response in age at maturity to temperature variation than did males from the other two SC populations. The pattern of population variation in plasticity within and among regions suggests that gene flow, which diminishes with distance in sailfin mollies, plays a critical role in constraining divergence in norms of reaction.

Dual-trait genomic analysis in highly stratified Arabidopsis thaliana populations using genome-wide association summary statistics.

Genome-wide association study (GWAS) is a powerful tool to identify genomic loci underlying complex traits. However, the application in natural populations comes with challenges, especially power loss due to population stratification. Here, we introduce a bivariate analysis approach to a GWAS dataset of Arabidopsis thaliana. We demonstrate the efficiency of dual-phenotype analysis to uncover hidden genetic loci masked by population structure via a series of simulations. In real data analysis, a common allele, strongly confounded with population structure, is discovered to be associated with late flowering and slow maturation of the plant. The discovered genetic effect on flowering time is further replicated in independent datasets. Using Mendelian randomization analysis based on summary statistics from our GWAS and expression QTL scans, we predicted and replicated a candidate gene AT1G11560 that potentially causes this association. Further analysis indicates that this locus is co-selected with flowering-time-related genes. The discovered pleiotropic genotype-phenotype map provides new insights into understanding the genetic correlation of complex traits.

Quantitative proteomics of dorsolateral prefrontal cortex reveals an early pattern of synaptic dysmaturation in children with idiopathic autism.

Autism spectrum disorder (ASD) is a developmental disorder with a rising prevalence and unknown etiology presenting with deficits in cognition and abnormal behavior. We hypothesized that the investigation of the synaptic component of prefrontal cortex may provide proteomic signatures that may identify the biological underpinnings of cognitive deficits in childhood ASD. Subcellular fractions of synaptosomes from prefrontal cortices of age-, brain area-, and postmortem-interval-matched samples from children and adults with idiopathic ASD vs. controls were subjected to HPLC-tandem mass spectrometry. Analysis of data revealed the enrichment of ASD risk genes that participate in slow maturation of the postsynaptic density (PSD) structure and function during early brain development. Proteomic analysis revealed down regulation of PSD-related proteins including AMPA and NMDA receptors, GRM3, DLG4, olfactomedins, Shank1-3, Homer1, CaMK2α, NRXN1, NLGN2, Drebrin1, ARHGAP32, and Dock9 in children with autism (FDR-adjusted P < 0.05). In contrast, PSD-related alterations were less severe or unchanged in adult individuals with ASD. Network analyses revealed glutamate receptor abnormalities. Overall, the proteomic data support the concept that idiopathic autism is a synaptopathy involving PSD-related ASD risk genes. Interruption in evolutionarily conserved slow maturation of the PSD complex in prefrontal cortex may lead to the development of ASD in a susceptible individual.

BIOTECHNOLOGICAL PROCESSING OF LOW-GRADE MEAT WITH ENZYME PREPARATIONS

Biotechnological methods for processing raw materials in the meat industry are associated with the creation of advanced technologies. Domestic and world experience indicate the advisability of using enzymes in the meat industry to accelerate the maturation processes and increase the quality of semi-finished products. In order to intensify technological processes, increase the nutritional and biological value of finished products, enzyme preparations are used in the process of processing low-grade meat raw materials. Currently, herbal enzyme preparations phytosin, papain, bromelain, etc. are widely used. Processing meat with enzymes improves the consistency of meat, softens the structure of rough and strong muscle fibers and connective tissue; helps to increase the degree of digestibility of the product; improves taste, smell and color and accelerates the ripening process of meat [1].Enzymes are participants in both traditional and new technological processes used in food production. The muscles of farm animals are characterized by low concentrations of intracellular enzymes. Some anatomical parts of the carcass are characterized by a high content of connective tissue. This determines the toughness of such meat and its slow maturation. Beef under normal conditions matures after 10-14 days of aging at a temperature of 2-4°C. After treatment with enzymes, the meat matures in 1-2 days. The main purpose of aging beef is to break down the muscle fiber structure and enhance the natural flavor of the meat [2].Processing low-grade meat with plant enzymes will make it possible to use parts of the carcass that are full in composition, but have naturally increased rigidity: meat from the hind legs, shoulder blades, and brisket. Processing meat with a plant enzyme preparation leads to significant destructive changes that provide an effective softening effect, and is consistent with the obtained assessments of physicochemical and functional technological properties [3].The use of enzyme preparations has a positive effect on the tenderness, juiciness, nutritional value of raw materials, the formation of the required level of water-binding and water-holding capacity, and improves organoleptic characteristics due to the targeted effect of plant enzymes and enzymatic preparations on the components of muscle tissue [4].

Pain Exposure and Brain Connectivity in Preterm Infants

Early-life exposure to painful procedures has been associated with altered brain maturation and neurodevelopmental outcomes in preterm infants, although sex-specific differences are largely unknown. To examine sex-specific associations among early-life pain exposure, alterations in neonatal structural connectivity, and 18-month neurodevelopment in preterm infants. This prospective cohort study recruited 193 very preterm infants from April 1, 2015, to April 1, 2019, across 2 tertiary neonatal intensive care units in Toronto, Canada. Structural connectivity data were available for 150 infants; neurodevelopmental outcomes were available for 123 infants. Data were analyzed from January 1, 2022, to December 31, 2023. Pain was quantified in the initial weeks after birth as the total number of invasive procedures. Infants underwent early-life and/or term-equivalent-age magnetic resonance imaging with diffusion tensor imaging to quantify structural connectivity using graph theory measures and regional connection strength. Eighteen-month neurodevelopmental outcomes were assessed with the Bayley Scales of Infant and Toddler Development, Third Edition. Stratifying by sex, generalized estimating equations were used to assess whether pain exposure modified the maturation of structural connectivity using an interaction term (early-life pain exposure × postmenstrual age [PMA] at scan). Generalized estimating equations were used to assess associations between structural connectivity and neurodevelopmental outcomes, adjusting for extreme prematurity and maternal education. A total of 150 infants (80 [53%] male; median [IQR] gestational age at birth, 27.1 [25.4-29.0] weeks) with structural connectivity data were analyzed. Sex-specific associations were found between early-life pain and neonatal brain connectivity in female infants only, with greater early-life pain exposure associated with slower maturation in global efficiency (pain × PMA at scan interaction P = .002) and local efficiency (pain × PMA at scan interaction P = .005). In the full cohort, greater pain exposure was associated with lower global efficiency (coefficient, -0.46; 95% CI, -0.78, to -0.15; P = .004) and local efficiency (coefficient, -0.57; 95% CI, -1.04 to -0.10; P = .02) and regional connection strength. Local efficiency (coefficient, 0.003; 95% CI, 0.001-0.004; P = .005) and regional connection strength in the striatum were associated with cognitive outcomes. In this cohort study of very preterm infants, greater exposure to early-life pain was associated with altered maturation of neonatal structural connectivity, particularly in female infants. Alterations in structural connectivity were associated with neurodevelopmental outcomes, with potential regional specificities.

Establishment and characterization of turtle liver organoids provides a potential model to decode their unique adaptations

Painted turtles are remarkable for their freeze tolerance and supercooling ability along with their associated resilience to hypoxia/anoxia and oxidative stress, rendering them an ideal biomedical model for hypoxia-induced injuries (including strokes), tissue cooling during surgeries, and organ cryopreservation. Yet, such research is hindered by their seasonal reproduction and slow maturation. Here we developed and characterized adult stem cell-derived turtle liver organoids (3D self-assembled in vitro structures) from painted, snapping, and spiny softshell turtles spanning ~175My of evolution, with a subset cryopreserved. This development is, to the best of our knowledge, a first for this vertebrate Order, and complements the only other non-avian reptile organoids from snake venom glands. Preliminary characterization, including morphological, transcriptomic, and proteomic analyses, revealed organoids enriched in cholangiocytes. Deriving organoids from distant turtles and life stages demonstrates that our techniques are broadly applicable to chelonians, permitting the development of functional genomic tools currently lacking in herpetological research. Such platform could potentially support studies including genome-to-phenome mapping, gene function, genome architecture, and adaptive responses to climate change, with implications for ecological, evolutionary, and biomedical research.

The Effects of Different Feed Ration Levels on Growth, Welfare Rating, and Early Maturation in Juvenile Atlantic Salmon (Salmo salar)

To investigate the possible effect of different feed ration levels on the growth, welfare, and early maturation of juvenile Atlantic salmon, 450 salmon parr with a mean (±standard error) initial weight of 51.6 g (±0.8) were reared in triplicate under three different feed ration levels for five months. The control group (100r) was fed every day, the 50r group was fed every other day, and the 33r group was fed every third day. In every group, 75 fish (half of the group) were individually tagged for monitoring of growth. The number of fin wounds was used as the welfare indicator, and to inspect the development of maturation, all fish were euthanized, and development of the gonads was monitored by visual inspection at the termination of the trial. The control group (100r) showed a significantly higher specific growth rate (0.90% day−1) compared to the lower fed groups (50r, 0.67% day−1 and 33r, 0.49% day−1); however, the growth difference was 21–24% less than expected solely on the difference in the amount of feed given to each group. The 100r group showed the highest welfare rating, and the 33r group the lowest possible, indicating more aggressive behaviour and fin biting due to feed restriction in the 33r group. No difference (p &gt; 0.45) was found in the development of maturation in females, but the combined numbers of males in maturity stages 2–5 showed an overall trend towards slower maturation in the 33r group compared to the 100r group. Although the present findings on the development of sexual maturation were subtle due to the limited time frame of the trial, the findings offer a foundation for future investigation into the relationship between the feed ration level and the development of sexual maturation in the rearing of juvenile Atlantic salmon.

Raman developmental markers in root cell walls are associated with lodging tendency in tef

Main conclusionUsing Raman micro-spectroscopy on tef roots, we could monitor cell wall maturation in lines with varied genetic lodging tendency. We describe the developing cell wall composition in root endodermis and cylinder tissue.Tef [Eragrostis tef (Zucc.) Trotter] is an important staple crop in Ethiopia and Eritrea, producing gluten-free and protein-rich grains. However, this crop is not adapted to modern farming practices due to high lodging susceptibility, which prevents the application of mechanical harvest. Lodging describes the displacement of roots (root lodging) or fracture of culms (stem lodging), forcing plants to bend or fall from their vertical position, causing significant yield losses. In this study, we aimed to understand the microstructural properties of crown roots, underlining tef tolerance/susceptibility to lodging. We analyzed plants at 5 and 10 weeks after emergence and compared trellised to lodged plants. Root cross sections from different tef genotypes were characterized by scanning electron microscopy, micro-computed tomography, and Raman micro-spectroscopy. Lodging susceptible genotypes exhibited early tissue maturation, including developed aerenchyma, intensive lignification, and lignin with high levels of crosslinks. A comparison between trellised and lodged plants suggested that lodging itself does not affect the histology of root tissue. Furthermore, cell wall composition along plant maturation was typical to each of the tested genotypes independently of trellising. Our results suggest that it is possible to select lines that exhibit slow maturation of crown roots. Such lines are predicted to show reduction in lodging and facilitate mechanical harvest.

First record of imposex in the horse conch, Triplofusus giganteus (Mollusca: Gastropoda: Fasciolariidae)

This study reports the first record of imposex in Triplofusus giganteus, a top predator in shallow water marine habitats. Imposex, an irreversible disorder in female caenogastropod snails caused by exposure to biocidal organotin compounds identified in vessel antifouling paints, was observed in three of four snails collected from Alligator Harbor, Florida, with affected females developing a non-functional male reproductive organ, the pseudopenis. The presence of imposex in this species is of particular concern for its long-term conservation, given its slow maturation, limited reproductive output, and the potential impact of environmental contamination.

Efficacy and mechanism of nourishing yin and purging fire therapy for central precocious puberty based on meta-analysis and network pharmacology.

Central precocious puberty (CPP) is due to the early activation of the hypothalamus-pituitary-gonadal axis, and its incidence is on the rise. A number of studies have shown that nourishing yin and purging fire (NYPF) therapy can be beneficial for CPP. Therefore, we conducted this review to investigate the efficacy, safety, and mechanism of NYPF therapy for CPP. Electronic databases including PubMed, the Cochrane Library, Web of Science, EMBASE, China National Knowledge Infrastructure, Chinese Biomedical Literature Database, Wan-fang Database, and China Scientific Technical Journals Database and 2 platforms including Clinical Trials and Chinese Clinical Trial Registry were searched for randomized controlled trials of NYPF therapy for CPP. A meta-analysis was conducted using RevMan 5.3 and Stata 17.0 software. The core herb pair of NYPF was identified by data mining using IBM SPSS Modeler 18.0 software. The active ingredients and targets of the core herb pair were obtained through the TCMSP database. The main targets of CPP were acquired form the GeneCards, Disgenet and TTD databases. A protein-protein interaction network was carried out to select the core genes by using STRING platform and Cytoscape 3.7.2 software. Metascape platform was used to conduct gene ontology (GO) and KEGG enrichment analysis. The results were verified utilizing molecular docking. A total of 23 studies were included. Meta-analysis shows the NYPF therapy could significantly improve the clinical efficacy rate and secondary sexual indicators (uterine volume, ovarian volume, breast nucleus diameter, follicular diameter), reduce TCM syndrome scores and serum sex hormone (FSH, LH, E2), and slow down bone age maturation compared to GnRHa therapy group. In addition, NYPF therapy was safe and has no obvious adverse events. Data mining revealed that the core herb pair of NYPF was "Anemarrhenae Rhizoma (Zhimu) - Phellodendri Chinensis Cortex (Huangbai)." Network pharmacology predicted that quercetin, kaempferol, beta-sitosterol, etc were the key components of Zhimu-Huangbai for treating CPP. The core targets were TP53, JUN, AKT1, ESR1, TNF, IL6, CCND1, MAPK1, BCL2, EGFR, IL1B, and PTGS2. They played a pivotal role in modulating multiple signaling pathways, such as Endocrine resistance, MAPK signaling pathway, and PI3K-Akt signaling pathway. This article revealed that NYPF therapy is effective and safe against CPP. The mechanism of the core herb pair of NYPF therapy for CPP through muti-components, muti-targets and muti-pathways.

Spatiotemporal kinetics of CAF-1-dependent chromatin maturation ensures transcription fidelity during S-phase.

Proper maintenance of epigenetic information after replication is dependent on the rapid assembly and maturation of chromatin. Chromatin Assembly Complex 1 (CAF-1) is a conserved histone chaperone that deposits (H3-H4)2 tetramers as part of the replication-dependent chromatin assembly process. Loss of CAF-1 leads to a delay in chromatin maturation, albeit with minimal impact on steady-state chromatin structure. However, the mechanisms by which CAF-1 mediates the deposition of (H3-H4)2 tetramers and the phenotypic consequences of CAF-1-associated assembly defects are not well understood. We used nascent chromatin occupancy profiling to track the spatiotemporal kinetics of chromatin maturation in both wild-type (WT) and CAF-1 mutant yeast cells. Our results show that loss of CAF-1 leads to a heterogeneous rate of nucleosome assembly, with some nucleosomes maturing at near WT kinetics and others showing significantly slower maturation kinetics. The slow-to-mature nucleosomes are enriched in intergenic and poorly transcribed regions, suggesting that transcription-dependent assembly mechanisms can reset the slow-to-mature nucleosomes following replication. Nucleosomes with slow maturation kinetics are also associated with poly(dA:dT) sequences, which implies that CAF-1 deposits histones in a manner that counteracts resistance from the inflexible DNA sequence, promoting the formation of histone octamers as well as ordered nucleosome arrays. In addition, we show that the delay in chromatin maturation is accompanied by a transient and S-phase-specific loss of gene silencing and transcriptional regulation, revealing that the DNA replication program can directly shape the chromatin landscape and modulate gene expression through the process of chromatin maturation.

Sleep spindle maturity promotes slow oscillation-spindle coupling across child and adolescent development.

The synchronization of canonical fast sleep spindle activity (12.5-16 Hz, adult-like) precisely during the slow oscillation (0.5-1 Hz) up peak is considered an essential feature of adult non-rapid eye movement sleep. However, there is little knowledge on how this well-known coalescence between slow oscillations and sleep spindles develops. Leveraging individualized detection of single events, we first provide a detailed cross-sectional characterization of age-specific patterns of slow and fast sleep spindles, slow oscillations, and their coupling in children and adolescents aged 5-6, 8-11, and 14-18 years, and an adult sample of 20- to 26-year-olds. Critically, based on this, we then investigated how spindle and slow oscillation maturity substantiate age-related differences in their precise orchestration. While the predominant type of fast spindles was development-specific in that it was still nested in a frequency range below the canonical fast spindle range for the majority of children, the well-known slow oscillation-spindle coupling pattern was evident for sleep spindles in the adult-like canonical fast spindle range in all four age groups-but notably less precise in children. To corroborate these findings, we linked personalized measures of fast spindle maturity, which indicate the similarity between the prevailing development-specific and adult-like canonical fast spindles, and slow oscillation maturity, which reflects the extent to which slow oscillations show frontal dominance, with individual slow oscillation-spindle coupling patterns. Importantly, we found that fast spindle maturity was uniquely associated with enhanced slow oscillation-spindle coupling strength and temporal precision across the four age groups. Taken together, our results suggest that the increasing ability to generate adult-like canonical fast sleep spindles actuates precise slow oscillation-spindle coupling patterns from childhood through adolescence and into young adulthood.

Differences in the genomic diversity, structure, and inbreeding patterns in wild and managed populations of Agave potatorum Zucc. used in the production of Tobalá mezcal in Southern Mexico.

Agave potatorum Zucc. locally known as Tobalá, is an important species for mezcal production. It is a perennial species that takes 10 to 15 years to reach reproductive age. Because of high demand of Tobalá mezcal and the slow maturation of the plants, its wild populations have been under intense anthropogenic pressure. The main objective of this study was to estimate the genome-wide diversity in A. potatorum and determine if the type of management has had any effect on its diversity, inbreeding and structure. We analyzed 174 individuals (105 wild, 42 cultivated and 27 from nurseries) from 34 sites with a reduced representation genomic method (ddRADseq), using 14,875 SNPs. The diversity measured as expected heterozygosity was higher in the nursery and wild plants than in cultivated samples. We did not find private alleles in the cultivated and nursery plants, which indicates that the individuals under management recently derived from wild populations, which was supported by higher gene flow estimated from wild populations to the managed plants. We found low but positive levels of inbreeding (FIS = 0.082), probably related to isolation of the populations. We detected low genetic differentiation among populations (FST = 0.0796), with positive and significant isolation by distance. The population genetic structure in the species seems to be related to elevation and ecology, with higher gene flow among populations in less fragmented areas. We detected an outlier locus related to the recognition of pollen, which is also relevant to self-incompatibility protein (SI). Due to seed harvest and long generation time, the loss of diversity in A. potatorum has been gradual and artificial selection and incipient management have not yet caused drastic differences between cultivated and wild plants. Also, we described an agroecological alternative to the uncontrolled extraction of wild individuals.

Life history impacts on infancy and the evolution of human social cognition.

Greater longevity, slower maturation and shorter birth intervals are life history features that distinguish humans from the other living members of our hominid family, the great apes. Theory and evidence synthesized here suggest the evolution of those features can explain both our bigger brains and our cooperative sociality. I rely on Sarah Hrdy's hypothesis that survival challenges for ancestral infants propelled the evolution of distinctly human socioemotional appetites and Barbara Finlay and colleagues' findings that mammalian brain size is determined by developmental duration. Similar responsiveness to varying developmental contexts in chimpanzee and human one-year-olds suggests similar infant responsiveness in our nearest common ancestor. Those ancestral infants likely began to acquire solid food while still nursing and fed themselves at weaning as chimpanzees and other great apes do now. When human ancestors colonized habitats lacking foods that infants could handle, dependents' survival became contingent on subsidies. Competition to engage subsidizers selected for capacities and tendencies to enlist and maintain social connections during the early wiring of expanding infant brains with lifelong consequences that Hrdy labeled "emotionally modern" social cognition.