Slow Weight Research Articles

Growth pattern during early infancy, body mass index during childhood and childhood asthma.

There is a lack of longitudinal studies of associations between growth from infancy to childhood and asthma development. The objective of the study was to investigate the effects of weight change during infancy, body mass index (BMI) and the interaction of these factors on the risk of childhood asthma. We enrolled children born in 2008 and 2009 at full-term and with normal birth weight. The weight change in infancy was grouped into slow, on-track and rapid. BMI status in childhood was stratified into low, normal and high groups and used as a time-varying variable. The outcome was asthma, defined as two or more diagnoses of asthma separated by at least 1year after 2 years of age. The risk of asthma was assessed using Cox proportional hazard regression, with adjustment for sex, residence area at birth, economic status and feeding types in infancy. Of 917,707 children born in Korea in 2008 and 2009, 271,871 were eligible for analysis. The risk of asthma was greater in groups with low birth weight (aHR 1.06, 95% CI 1.04 to 1.08), rapid body weight change during early infancy (aHR 1.08, 95% CI 1.07 to 1.10) and high BMI during childhood (aHR 1.06, 95% CI 1.04-1.08). The interaction of weight change during early infancy with BMI during childhood was significant for asthma (p < .01). Rapid weight gain in infancy was associated with lower risk of asthma in those with low BMI during childhood; had no association with asthma in those with normal BMI during childhood; and was associated increased asthma risk in those with high BMI during childhood-aHR 1.26 (95% CI 1.19 to 1.33) and aHR 1.33 (95% CI 1.12 to 1.56) compared with on-track and slow infant weight gain, respectively. Low birth weight, high BMI during childhood and, in those with high childhood BMI, rapid weight gain during early infancy are associated with increased risk of childhood asthma.

Thermogravimetric Analysis of Marine Macroalgae Waste Biomass as Bio-Renewable Fuel

Macroalgae are considered as the 3rd generation of biofuels and a future feedstock for biorefinery. This research aims to provide simple and dependable analytical techniques for measuring the thermal characteristics of dried seaweed. The main objective was to investigate the thermal characteristics of four seaweed species utilizing a thermogravimetric analyzer. The seaweeds Gracilaria fisheri, Caulerpa lentillifera, Ceramium rubrum, and Eucheuma cottonii were collected from the Pahang state of Peninsular Malaysia. The calorific value of the samples was revealed by using a calorimeter. Ceramium rubrum showed the highest calorific value, while Gracilaria fisheri had the most negligible calorific value among the selected samples. The thermogravimetric analysis (TGA) data revealed that the most significant weight loss for this biomass occurred between 160 and 300° for the selected species. Gracilaria fisheri has shown the highest decomposition with the minor residue at 30.26%, whereas Caulerpa lentillifera has a slow weight loss rate in the mentioned range. SEM analysis has been used to perform the morphology of samples, which shows differences in the concentration of epiphytic diatoms with different structural shapes. Based on the results, macroalgae is a promising sustainable biomass feedstock for biofuel application.

Biological characterization of cold-adapted SARS-CoV-2 variants

Introduction. The emergence of new epidemiologically significant variants of SARS-CoV-2 has shifted emphasis to development of a live vaccine, which would be able to provide protection against a wide range of antigenic variants of the virus. The aim of the study was to obtain SARS-CoV-2 variants attenuated through cold adaptation and to provide their biological characterization.Materials and methods. The Dubrovka laboratory strain of SARS-CoV-2 and its variants were cultured on Vero and Calu-3 cells. The virus quantification was performed by virus titration in Vero cells and by real-time reverse transcription-polymerase chain reaction. SARS-CoV-2 virions were analyzed using transmission electron microscopy. Genome sequences of the virus were identified by nanopore sequencing. The attenuation (att) phenotype of SARS-CoV-2 variants was identified using Syrian hamsters as an animal model for COVID-19. Results. Cold-adapted (ca) SARS-CoV-2 variants – Dubrovka-ca-B4 and Dubrovka-ca-D2 were produced by continued passaging of the Dubrovka strain in the Vero cell culture at the temperature being gradually decreased to 23ºC and by subsequent cloning. Up to 20 nucleotide substitutions and 18 amino acid substitutions were detected in genomes of ca-variants. Ca-variants, as distinct from the parent Dubrovka strain, actively replicated at 23ºC, while the Dubrovka-ca-D2 variant had a temperature-sensitive (ts) phenotype (did not replicate at 39ºC). Ca-variants of the virus replicated poorly at 37ºC in the Calu-3 human lung cell culture, which, along with the ts-phenotype, can be a marker of virus attenuation for humans. In the intranasally infected Syrian hamsters, ca-variants of the virus demonstrated an attenuation phenotype: they did not cause loss of appetite, fatigue, drowsiness, did not slow down weight gain, replicating much more slowly in the lungs and brain compared to the virulent Dubrovka strain. Conclusion. The obtained attenuated SARS-CoV-2 ca-variants, Dubrovka-ca-B4 and Dubrovka-ca-D2, should be studied further as candidate vaccine strains for a live attenuated vaccine against COVID-19.

Impact of a Novel Diabetes Prevention Intervention for Early Slow Weight Loss Responders Among Adults With Prediabetes: An Adaptive Trial.

Some people are slower to respond during lifestyle interventions. An adaptive "rescue" intervention may improve outcomes among slow responders. The impact of a worksite rescue intervention for early slow responders was evaluated. Employees ≥21 years old with prediabetes were stratified to intervention using a 2.5% weight loss (%WL) threshold at week 5. Outcomes were assessed at baseline and at 4 months using mixed-effect and linear regression models. Significant improvement occurred in mean %WL, glycemia, total cholesterol, and triglycerides in the standard compared with the adaptive (Group Lifestyle Balance Plus [GLB+]) intervention (all P≤ 0.01). However, GLB+ participants also experienced a significant reduction in %WL and glycemia (all P < 0.01). The %WL at week 5 significantly predicted %WL at 4 months (P < 0.0001). The between-group difference of 4-month %WL was not significant for someone achieving 2.5%WL at week 5. Diabetes prevention programs should consider weight loss success following 1 month of treatment and offer a rescue intervention to early slow weight loss responders.

Adherence to a Healthy Beverage Score Is Associated with Lower Frailty Risk in Older Adults.

Many beverages include bioactive components and energy but are frequently not considered in diet quality estimations. We examined the association of a healthy beverage score (HBS) with incident frailty in older adults from the Seniors-ENRICA-1 cohort. We used data from 1900 participants (mean ± SD age 68.7 ± 6.4 years, 51.7% women), recruited in 2008–2010 and followed-up until 2012 assessing food consumption at baseline with a validated diet history. The HBS was higher for increasing consumption of low fat milk, tea/coffee, lower consumption of whole milk, fruit juice, artificially sweetened beverages, sugar-sweetened beverages, and moderate intake of alcohol. Frailty was considered as having ≥3 criteria: exhaustion, low-physical activity, slow gait speed, weakness, and weight loss. We performed logistic regression analyses adjusted for potential confounders. During a 3.5 y mean follow-up, 136 new cases of frailty occurred. Compared to the lowest sex-specific HBS tertile, the fully adjusted odds ratio (95% confidence interval) of frailty was 0.59 (0.38, 0.92) in the intermediate tertile, and 0.52 (0.31, 0.88) in the highest tertile, p trend = 0.007. Results for slow gait speed were 0.79 (0.58, 1.07) and 0.71 (0.51–0.99), p trend = 0.033. Therefore, adherence to HBS was inversely associated with incident frailty and slow gait speed. HBS can help on the beverage quality evaluation, highlighting beverage importance as contributors to diet and to health.

Association of protein-energy partitioning with body weight and body composition changes in adolescents with severe obesity.

Body composition and protein-energy partitioning changes are important factors of body weight regulation, but have not been studied in the context of clinical obesity treatment setting. Thus, the aim of the present study was to investigate the pattern of body weight loss, body composition, and energy partitioning changes during a 9-month multidisciplinary weight loss program and 4-month follow up and to test the associations among these changes in adolescents with severe obesity. Twenty-five adolescents (14.1 ± 1.5 years old; 13 girls) with severe obesity joined a pediatric obesity center for a 9-month inpatient multidisciplinary weight loss program. All participants performed body composition assessment (i.e. fat mass-FM, and fat-free mass-FFM) and completed a 36-h session in indirect calorimetric chamber before the start (T0), at the end of the intervention (T1) and 4 months follow-up to the intervention (T2). The protein-energy partitioning (P ratio) was calculated as urinary nitrogen loss/total energy expenditure over 24 hours. 9-month individualized multidisciplinary weight loss program consisting of lifestyle education, psychological support, physical activity, and dietary intervention. Initial P ratio was positively associated with changes in body weight from T0 to T1 (p = 0.038). The changes in FFM/FM were negatively associated with body weight changes in boys (p = 0.006) from T0 to T1 and in girls (p < 0.001) from T1 to T2. Urinary nitrogen excretion (p < 0.001) and total energy expenditure (p < 0.001) significantly decreased during the weight loss program while the P ratio did not significantly change. The present results suggest that baseline and changes in energy partitioning may be associated with changes in body weight in adolescents with severe obesity. In addition, sexual dimorphism in these patterns of change suggest the need for specific dietary and physical activity strategies in boys and girls to optimize body weight loss and to prevent or slow weight regain.

The key amino acid sites 199–205, 269, 319, 321 and 324 of ALV-K env contribute to the weaker replication capacity of ALV-K than ALV-A

BackgroundAvian leukosis virus (ALV) is an infectious retrovirus, that mainly causes various forms of tumours, immunosuppression, a decreased egg production rate and slow weight gain in poultry. ALV consists of 11 subgroups, A–K, among which ALV-K is an emerging subgroup that has become prevalent in the past 10 years. Most ALV-K isolates showed weak replication ability and pathogenicity. In this study, the weak replication ability of ALV-K was explored from the perspective of the interaction between ALV-K gp85 and the Tva receptor.MethodsFourteen soluble recombinant ALV-A/K gp85 chimeric proteins were constructed by substituting the sequence difference regions (hr1, hr2 and vr3) of the ALV-A gp85 protein with the skeleton ALV-K gp85 protein for co-IP and competitive blocking tests.ResultsThe binding capacity of ALV-K gp85 to Tva was significantly weaker than that of ALV-A gp85 (P < 0.05) and the key amino acid sites 199–205, 269, 319, 321 and 324 of ALV-K env contributed to the weaker replication capacity of ALV-K than ALV-A.ConclusionsThis is the first study to reveal the molecular factors of the weak replication ability of ALV-K from the perspective of the interaction of ALV-K gp85 to Tva, providing a basis for further elucidation of the infection mechanism of ALV-K.

Effects of Lead and Cadmium Combined Heavy Metals on Liver Function and Lipid Metabolism in Mice.

Although a large number of studies have been conducted on lead (Pb) and cadmium (Cd) exposure individually, information regarding the toxicity of combined Pb and Cd exposure is relatively limited. The present study aims to investigate the toxicity of Pb-Cd combination exposure and the corresponding mechanism. A heavy metal exposure model was established in mice by subcutaneous intragastric administration of Pb-Cd (50:1) for 35days. Body weight, diet, hair state, mental state, liver index, haematological index, biochemical indicators and pathological section analysis were used to comprehensively evaluate toxicity. Then, classical oxidative stress indexes and lipidomics techniques were used to explore the potential mechanism. The results showed that Pb-Cd caused the mice to have low appetite, poor spirit, significantly reduced activity, slow weight gain and irritated or drying hair. Pb-Cd also caused liver enlargement, significantly increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities, and resulted in pathological changes to the liver. Prolonged Pb-Cd exposure led to significantly increased thrombocyte haematocrit (PCT), white blood cell (WBC), platelet (PLT) and monocyte (MON) counts and decreased red blood cell (RBC), haemoglobin (HGB), haematocrit (HCT) and lymphocyte (LYM) counts. Pb-Cd increased oxidative stress by increasing the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) and the content of malondialdehyde (MDA). Finally, Pb-Cd triggered lipid metabolism disorders by regulating linoleic acid, sphingolipid and glycerolipid metabolism.

Insight into the influence of calcium on the co-pyrolysis of coal and polystyrene

In this paper, the influence of calcium on the co-pyrolysis of coal and polystyrene (PS) is investigated by reactive molecular dynamics (ReaxFF MD) method. The experimental results show the same trend of products variation as the pyrolysis simulation. The results show that the presence of calcium in the coal sample significantly promotes the co-pyrolysis process. The addition of calcium greatly enhances the rapid cracking of coal/PS into small molecular fragments within 2000–2600 K, leading to the high yield of light tar. The slow weight loss of oxygen-containing char and high CO yield, as well as the sharp rise of oxygen-free tar, suggest the promotion of calcium on the conversion of tar to char in coal pyrolysis and the positive effect on PS pyrolysis. Moreover, the delayed release of OH radicals in coal and early release of H radicals in PS during coal(Ca)/PS co-pyrolysis present the strong binding effect of Ca-containing species on free radicals in coal and the promotion effect of calcium on PS pyrolysis. Furthermore, the low C8H8 yield in the early stage during coal(Ca)/PS co-pyrolysis, along with the phenomenon that H radicals are released early, proves that calcium-containing compounds enhance benzene ring dehydrogenation or the breaking of CC bonds in PS. By using the first-order reaction model, the kinetic parameters for the co-pyrolysis of coal/PS and coal(Ca)/PS are quantitatively determined.

Huangqi Guizhi Wuwu Decoction can prevent and treat oxaliplatin-induced neuropathic pain by TNFα/IL-1β/IL-6/MAPK/NF-kB pathway

Objective: This study explored the effects and mechanisms of Huangqi Guizhi Wuwu Decoction on chemotherapy-induced neuropathic pain (CINP).Methods: Bodyweight and related behavioral testing of the rat model were utilized to investigate the effects of Huangqi Guizhi Wuwu Decoction on CINP. ELISA was used to measure the levels of TNF-α, IL-1β, and IL-6, in the serum of chronic CINP rats. Immunohistochemistry and Western blot analysis were performed to detect the expression of MAPK pathway related-proteins namely ERK1/2, p38, and JNK, and the expression of downstream essential proteins such as c-Fos, CREB, and NF-κB.Results: Body weight and related behavioral testing of the rat model suggests that Huangqi Guizhi Wuwu Decoction can improve the slow weight gain of oxaliplatin-induced chronic CINP model rats and effectively prevent and treat oxaliplatin-induced regular CIPN rat model of hyperalgesia. It can also oppress the mechanical pain threshold, cold pain threshold, and heat pain threshold decreased. Furthermore, by ELISA, immunohistochemistry, and western blot analysis, we found that Huangqi Guizhi Wuwu Decoction can down-regulate the levels of TNF-α, IL-1β, and IL-6 in the serum of chronic CINP rats induced by oxaliplatin. It also suppresses the expression of MAPK pathway related-proteins ERK1/2, p38, and JNK. This results in a decrease in the expression of downstream essential proteins, c-Fos, CREB, and Nf-κB.Conclusions: In conclusion, we found that Huangqi Guizhi Wuwu Decoction can combat nerve cell injury, reduce pain sensitization, and prevent and repair the damage of nerve cells in the oxaliplatin CINP model rats via TNFα/IL-1β/IL-6/MAPK/NF-kB pathway.

Contribution of hippocampal BDNF/CREB signaling pathway and gut microbiota to emotional behavior impairment induced by chronic unpredictable mild stress during pregnancy in rats offspring.

BackgroundNumerous studies have shown that exposure to prenatal maternal stress (PMS) is associated with various psychopathological outcomes of offspring. The accumulating evidence linking bacteria in the gut and neurons in the brain (the microbiota-gut-brain axis) has been aconsensus; however, there is a lack of research on the involvement mechanism of gut microbiota in the regulation of the BDNF/CREB signaling pathway in the hippocampus of prenatally stressed offspring.MethodsPregnant rats were subjected to chronic unpredictable mild stress (CUMS) to establish the prenatal maternal stress model. The body weight was measured and the behavioral changes were recorded. Offspring were tested to determine emotional state using sucrose preference test (SPT), open-field test (OFT) and suspended tail test (STT). Gut microbiota was evaluated by sequencing the microbial 16S rRNA V3–V4 region, and the interactive analysis of bacterial community structure and diversity was carried out. The expression of hippocampal BDNF, TrkB and CREB mRNA and proteins were respectively measured using RT-PCR and Western blotting.ResultsPrenatal maternal stress increased maternal plasma corticosterone levels, slowed maternal weight gain and caused depression-like behaviors (all P < 0.05). In offspring, prenatal maternal stress increased plasma corticosterone levels (P < 0.05) and emotional behavior changes (depression-like state) were observed (P < 0.05). The species abundance, diversity and composition of the offspring’s gut microbiota changed after the maternal stress during pregnancy (P < 0.05). Compared with the control group’s offspring, the species abundance of Lactobacillaceae was dropped, while the abundance of the Muribaculaceae species abundance was risen. Concurrent, changes in the hippocampal structure of the offspring and decreases in expression of BDNF/CREB signaling were noted (P < 0.05).ConclusionsPrenatal maternal stress leads to high corticosterone status and abnormal emotion behavior of offspring, which may be associated with the abnormal BDNF/CREB signaling in hippocampus of offspring caused by the change of gut microbiota composition.

Comparative Response of Mango Fruit towards Pre- and Post-Storage Quarantine Heat Treatments

The present study investigates the comparative effect of pre- and post-storage quarantine heat treatments (hot water treatment (HWT) and vapor heat treatment (VHT)) on the post-harvest performance of the mango fruit cv. ‘Chenab Gold’. The results indicate that the application of HWT at 48 °C for 60 min or VHT at 47 °C for 25 min after 21 days under cold storage enhanced the ethylene production and fruit weight loss, while decreasing fruit firmness and vitamin C content. Noticeably, the post-storage heat treatments ruptured the fruit and destroyed their market value. However, fruit treated with HWT or VHT at harvest exhibited slow weight loss, better skin color, and maintained biochemical attributes as compared to the control when kept under ambient storage conditions. Taken together, the application of hot water before storage has a positive influence on mango fruit quality, while post-storage heat treatment has a devastating impact upon fruit quality and shelf life, cancelling its potential commercial application.

Identification of MMACHC and ZEB2 mutations causing coexistent cobalamin C disease and Mowat-Wilson syndrome in a 2-year-old girl

Cobalamin C (cblC) disease and Mowat-Wilson syndrome (MWS) are rare hereditary diseases. To date, there have been no reports of people suffering from these two genetic diseases, or whether there is any correlation between the two diseases. We reported a 2-year-old girl with both cblC disease and MWS. The patient initially manifested as slow weight gain, hypotonia, broad nasal bridge, high forehead, high palate arch, ear crease, patent ductus arteriosus, atrial and ventricular septal defect and bilateral mild ventriculomegaly in the neonatal period. However, as the baby grew older, the typical facial features became more prominent, and overall developmental delays were noted at the subsequent follow-up, with the motor and cognitive development significantly lagging behind that of other children of the same age. At 26 days old, laboratory tests revealed remarkably elevated levels of serum homocysteine, C3/C2 and urine organic acid. Whole-exome sequencing detected compound heterozygous variants in MMACHC, including one previously reported mutation [c.609G > A (p.W203X) and a novel missense mutation[ c.643 T > C (p.Y215H)]. The computer simulations of the protein structure analysis of the novel missense mutation showed the variant p.Y215H replaced a neutral amino acid with a strongly basic lysine, which broken the local structure by changing the carbon chain skeleton and decreasing the interaction with adjacent amino acid. This is expected to damage the utilization of vitamin B12 and influence the synthesis of AdoCbl and MeCbl, contributing to its pathogenicity. Thus, clinical and genetic examinations confirmed the cblC disease. Another heterozygous variant in ZEB2 [NM_014795; loss1(exon:2–10)(all); 127901 bp] was detected by whole-exome sequencing. The heterozygous 3.04 Mb deletion in EB2 [GRCH37]del(2)(q22.2q22.3) (chr2:142237964–145274917) was also confirmed by genome-wide copy number variations (CNVs) scan, which was pathogenic and led to the diagnosis of Mowat-Wilson syndrome. The biochemical indicators associated with cblC disease in the patient were well controlled after treatment with vitamin B12 and betaine. Here, a patient with coexisting cblC disease and MWS caused by different pathogenic genes was reported, which enriched the clinical research on these two rare genetic diseases.

A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance.

The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host. A high-calorie diet has been shown to aggravate pneumonia and delay recovery, especially in children. However, the underlying mechanisms remain unclear. Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats. In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination. Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage. Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells. Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats. Consequently, the levels of SCFAs, especially acetate, propionate, and butyrate, were significantly decreased following the intervention of a high-calorie diet. More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation. Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.

A Study on the Time-Effect and Dose-Effect Relationships of Polysaccharide from Opuntia dillenii against Cadmium-Induced Liver Injury in Mice.

The purpose of this study was to evaluate the protective effect of Opuntia dillenii (Ker-Gaw) Haw. polysaccharide (ODP) against cadmium-induced liver injury. Cadmium chloride (CdCl2) was used to construct a mice evaluation model, and the indicators chosen included general signs, liver index, biochemical indicators, blood indicators, and pathological changes. A dose of 200 mg/kg ODP was applied to the mice exposed to cadmium for different lengths of time (7, 14, 21, 28, and 35 days). The results showed that CdCl2 intervention led to slow weight growth (reduced by 13–20%); liver enlargement; significantly increased aspartate aminotransferase (AST, 45.6–52.0%), alanine aminotransferase (ALT, 26.6–31.3%), and alkaline phosphatase (ALP, 38.2–43.1%) levels; and significantly decreased hemoglobin (HGB, 13.1–15.2%), mean corpuscular hemoglobin (MCH, 16.5–19.3%), and mean corpuscular hemoglobin concentrations (MCHC, 8.0–12.7%) (p < 0.01). In addition, it led to pathological features such as liver cell swelling, nuclear exposure, central venous congestion, apoptosis, and inflammatory cell infiltration. The onset of ODP anti-cadmium-induced liver injury occurred within 7 days after administration, and the efficacy reached the highest level after continuous administration for 14 days, a trend that could continue until 35 days. Different doses (50, 100, 200, 400, and 600 mg/kg) of ODP have a certain degree of protective effect on cadmium-induced liver injury, showing a good dose–effect relationship. After 28 days of administration of a 200 mg/kg dose, all pathological indicators were close to normal values. These findings indicated that ODP had positive activity against cadmium-induced liver injury and excellent potential for use as a health food or therapeutic drug.

Abnornal connection of the left partial pulmonary vein to the innominate vein: A clinical case and literature review

Background: The left partial abnormal pulmonary venous connection is a very rare and easily missed congenital anomaly. If not handled properly, this defect can cause many serious disorders in the development of the child. Surgery to return the pulmonary vein to the left atrium is the solution to this situation.&#x0D; Methods: We report a case of a left partial abnormal pulmonary vein draining into the left innominate vein with a perimembranous ventricular septal defect that was diagnosed and operated on at the Pediatric Cardiovascular Surgery Department, Heart center, E hospital. In this case, we discuss methods of diagnosis, treatment, and review the medical literature on this abnormal form of pulmonary veins.&#x0D; Results: Female, 15 months old, history: second child, normal delivery, full-term, birth weight 3000g, no asphyxia, no cyanosis after birth; detected congenital heart from 30 weeks gestation. After birth, the child can eat, suckle, and have normal mental-motor development. Recently, the child ate poorly, had slow weight gain, went to the provincial hospital for examination, was transferred to E hospital's cardiovascular center. Ultrasound and cardiac catheterization detected perimembranous ventricular septal defect and left partial pulmonary vein abnormality. We performed a total surgical repair including patching the ventricular septal defect and transferring the pulmonary vein to the left atrium through the incision between the sternum. Postoperative echocardiography gave good results. The patient was discharged from the hospital 9 days after surgery.&#x0D; Conclusion: Surgery to transfer the pulmonary vein to the left atrium for the abnormality of the left partial pulmonary vein connection has good results.

The Frenotomy Efficacy in Gaining Weight of Exclusively Breastfed Infant with Ankyloglossia

Background: Ankyloglossia, or tongue-tie, is a condition in which the tongue is attached to the floor of the mouth through the frenulum, causing limited tongue movement. Short frenulum variations cause reduced anterior tongue movement resulting in breastfeeding, swallowing, articulation, and orthodontics problems. These abnormalities in infants cause breastfeeding problems that affect infants' weight gain, malocclusion, and speech disorders. Frenotomy in infant ankyloglossia is performed if there are problems with breastfeeding, poor sucking, slow weight gain, and recurrent mastitis. With this indication, we need to observed the efficacy of frenotomy done before the age of 1 month, and the frenotomy done at 1-3 months old in gaining weight of exclusively breastfed infants with ankyloglossia. Materials and Methods: A observational retrospective cohort method from patient medical records in a private hospital in Jakarta, Indonesia. The research subject is a total sampling patient medical records secondary data and collected 68 subjects. The Subjects are divided into two groups, the first group was patients whose frenotomy was done before the age of 1 month, and the second group was patients whose frenotomy was done at 1-3 months old. We assessed the weight gain between the two groups. The data were analyzed and compared the percentage of weight gain before and after frenotomy. Results: The percentage of weight gain before and after frenotomy shows that in the first group, there was a percentage gain of 11.4% in body weight of the subjects and during post-frenotomy control, the percentage of weight gain in this group increased to 111, 4%. In the second group, the percentage of weight gain before the frenotomy was 70.6%, while the post-frenotomy control was only 57.7%. Conclusion: The study demonstrated the efficacy of frenotomy in gaining weight of exclusively breastfed infants with ankyloglossia. There was a significant difference in the weight gain of infants with ankyloglossia who underwent frenotomy at the age of exclusive breastfeeding, before the age of 1 month and between the ages of 1 to 3 months.

Sodium silicate treatment promotes suberin poly phenolic and silicon deposition, and enhances hardness and brittleness at muskmelon wounds

Silicon can enhance plant resistance to biotic stress by directly inhibiting pathogens, forming physical barriers, and inducing the synthesis of defense compounds. However, no study is available on silicon on fruit wound healing. Here, we found that sodium silicate enhanced the activities of phenylalanine ammonia-lyase, 4-coumarate coenzyme A ligase, and cinnamate-4-hydroxylase, and elevated levels of cinnamic, ρ-coumaric, caffeic, ferulic, sinapic acids, and total phenols at fruit wounds. Sodium silicate accelerated the deposition of suberin poly phenolic and silicon at wounds, increased the hardness and brittleness and reduced their resilience of the healing tissues, which slowed weight loss by wounded fruit, and disease index in fruit subjected to Trichothecium roseum. Taken together, sodium silicate may promote wound healing in muskmelons by activating phenylpropanoid pathway, accelerating suberin poly phenolic and silicon deposition at wounds, and increasing the hardness and brittleness of healing tissues.

Gastric Transposition for Repair of Long-Gap Esophageal Atresia: Indications, Complications, and Outcome of Minimally Invasive and Open Surgery

Background: Gastric transposition (GT) is a possible option for esophageal replacement in long-gap esophageal atresia (LGEA). The present study aims to report and compare indications and outcome of laparoscopic-assisted GT (LAGT) versus open (OGT) GT for LGEA repair. Methods: Retrospective single-center analysis of all LGEA patients undergoing GT between 2002 and 2021. Results: Thirty-one children with LGEA underwent GT. Of these, 19 underwent LAGT (mean weight at surgery 5.6 kg; mean age 167 days) and 12 underwent OGT (6.1 kg; 233 days). Indications for OGT were previous surgery (n = 7), associated severe cardiac malformations (n = 4), and a simultaneous resection of a choledochal cyst (n = 1). The conversion rate was 1. The two procedures (LAGT/OGT) differed in anesthetic time (308/350 min), duration of ventilation (5.1/5.3 days), hospital stay (34/32 days), and complications (22/15). None of the differences reached statistical significance. Outcome was also comparable: completely oral nutrition uptake in 66%/73%, slow weight gain in the low centiles in both groups, no patient developed dumping syndrome, symptomatic reflux was seen in 1 patient after OGT. Conclusion: In our cohort, LAGT for repair of LGEA provided similar outcomes as open surgery. The minimally invasive approach preserves thoracal structures, prevents additional thoracotomy or laparotomy, and is faster. To realize LAGT, a postpartal treatment concept including gastrostomy placement via a microincision to minimize adhesions is essential. The open surgical approach should be considered in cases of previous extensive surgical attempts of EA correction causing severe adhesions as well as associated anomalies or genetic syndromes causing hemodynamic instability.

Impact of Consumption of Two Street Foods (Tuna Garba and Rice with Eggplant Sauce) on Vital Organs in the Wistar Rat (Rattus norvegicus)

Street foods are seen as a public health problem due to lack of infrastructure and basic services, difficulty in controlling the plethora of street food sales operations due to their diversity, their mobility, and their temporary nature. The objective of this study is to show the impact of the consumption of two street foods (Garba with tuna and rice with eggplant sauce) consumed in Côte d'Ivoire on the health of the Ivorian population. To contribute to the nutritional and health security of the population, the consumption of these dishes has been demonstrated in young Wistar rats (Rattus norvegicus). Thus, we hypothesized that the consumption of these street foods would have a deleterious effect on consumer health. However, five days 20 young rats of male and female sexes aged 50±5 days and weighing an average of 55±5 g were acclimatized and fed on a formulated isocaloric diet rich in herring fish (Clupea harengus) animal protein. After the acclimatization phase, four batches of rats of five rats per cage were fed respectively with diets (DWP, CDHF, GWTF and RES). The results showed that juvenile rats fed the CDHF and RES diet gained a lot of weight (5.66 ± 0.34 g/d and 5.16 ± 0.58 g/d) while those fed the GWTF diet had a progressive, slow weight gain. (2.32 ±0.23 g/d) and batch of rats fed without protein (DWP) observed considerable weight loss (-1.07±0.06 g/d). In terms of biological value, the results demonstrate that rats fed the RES diet have a higher protein availability (84.23 ±0.02%) than rats fed the GWTF diet (54.31 ±0.06 %). It follows from this analysis that it is necessary to combine GWTF with other protein-rich foods to compensate for the nutritional deficits caused by its ingestion. In young rats fed experimental diets, biometric research on vital organs (heart, liver, spleen and kidneys) revealed no abnormalities.