The resistance of Al0.5Cr0.9FeNi2.5V0.2 high entropy alloy (HEA) to hydrogen embrittlement was investigated by slow strain rate test (SSRT) and fracture surface was examined through scanning electron microscope. Compared with other ultra-high strength steels, Al0.5Cr0.9FeNi2.5V0.2 showed insignificant strength loss after hydrogen charging. The fracture surface of hydrogen charged specimens mainly consisted of dimples and no intergranular morphology was observed. The coupling effect of the dispersed nano-structured precipitates and high-density dislocations in Al0.5Cr0.9FeNi2.5V0.2 improves the resistance to hydrogen-induced strength loss.