Elymus Trachycaulus Research Articles

Forage yield of simple and complex grass–legume mixtures under two management strategies

Foster, A., Vera, C. L., Malhi, S. S. and Clarke, F. R. 2014. Forage yield of simple and complex grass–legume mixtures under two management strategies. Can. J. Plant Sci. 94: 41–50. Limited information is available on the dry matter production, protein content and species composition of complex mixtures of introduced perennial forage crop species for hay and pasture in the moister regions of Saskatchewan. A field experiment was sown on 2008 May 27 at Melfort, Saskatchewan, Canada, on a thick Black Chernozem (Udic Boroll) silty clay soil, to compare the effects on dry matter yield (DMY), protein content and species composition of perennial forage crop monocultures and mixtures, under a two-cut and a three-cut management system, in 2009, 2010, 2011 and 2012. Eleven treatments consisted of monocultures of hybrid bromegrass (Bromus riparius Rehm.×Bromus inermis Leyss.), smooth bromegrass (Bromus inermis Leyss.), crested wheatgrass [Agropyron cristatum (L.) Gaertn], intermediate wheatgrass [Agropyron intermedium (Host.) Beauv.] and alfalfa (Medicago sativa L.); simple mixtures of each of these individual grass species with alfalfa; a more complex mixture of all these grass species and alfalfa, and a very complex mixture consisting of the complex mixture plus meadow bromegrass (Bromus riparius Rehm.), orchardgrass (Dactylis glomerata L.), tall fescue [Festuca arundinacea (Schreb.) Wimm.], timothy (Phleum pratense L.) and slender wheatgrass [Elymus trachycaulus (Link) Gould ex Shinners]. The alfalfa was inoculated with rhizobium, and no fertilizer was added to any treatment during the course of the study. The two-cut system yielded higher than the three-cut system in all years. In 2009, the first cutting year, all treatments, especially under the two-cut system, produced similar forage DMY, with monoculture grasses yielding as much as that of their mixture with alfalfa. In 2010, 2011 and 2012, however, monoculture alfalfa and grass–alfalfa treatments had higher DMY than the monoculture grasses. Monoculture alfalfa also showed superior DMY than most grass–alfalfa mixtures, especially during the last 2 yr of the study. In conclusion, the inclusion of alfalfa in forage mixtures, grown in unfertilized soil, significantly increased forage yield, especially after the first production year, and these grass–alfalfa mixtures yielded almost as much as monoculture alfalfa. Also, the inclusion of alfalfa significantly increased the forage protein content over the grass alone treatments. Hybrid bromegrass, grown alone or in mixture with alfalfa, yielded similar to the other grasses tested, while crested wheatgrass did not compete as well as other main grasses, grown alone or in mixture with alfalfa. In this study, the use of complex or very complex mixtures of introduced grasses with alfalfa did not increase DMY over the simple grass–alfalfa mixtures.

Effect of Row Spacing on Seed Yield and Yield Components of Five Cool‐Season Grasses

ABSTRACTIncreasing seed yield and quality of key cool‐season perennial grasses is an important component of meeting China's environmental, food security, and urban beautification goals. Determining a suitable seed producing region and developing best management practices for this region with specific, high priority grasses will contribute to the availability of seed for reducing desertification, increasing livestock production, and improving urban living conditions. Previous research in other areas has addressed various agronomic practices on some grasses, but none has evaluated optimal row spacings for producing high yields of high quality seed in conditions similar to the Hexi Corridor, currently a major seed producing area in China for other seed crops. We compared our field trials in this area with those of other regions in China and the world to determine if this area would be suitable as a grass seed production center. Five important perennial, cool‐season grasses {[Elymus kamoji (ohwi) S.L. Chen, slender wheatgrass [Elymus trachycaulus (Link) Gould ex Shinners ssp. Trachycaulus], smooth bromegrass (Bromus inermis Leyss), Siberian wildrye (Elymus sibiricus L.), and Chinese sheepgrass [Leymus chinensis (Trin) Tzvel]} were evaluated from 2010 to 2012 under four row spacing treatments (30, 50, 70, and 90 cm). Results showed all five grasses have potential for high seed production in this region, with yields equivalent to or significantly higher than other areas. Optimal row spacing for Elymus kamoji and smooth bromegrass was 30 cm while a 50 cm row spacing was better for seed production and weed control for slender wheatgrass, Siberian wildrye, and Chinese sheepgrass. This work will allow multinational seed companies, development agencies, and government program directors to better evaluate the potential economic sustainability of grass seed production in this region compared to importing seed from other regions of the world.

Effects of low levels of herbicides on prairie species of the Willamette Valley, Oregon

The relative sensitivity of 17 noncrop plant species from Oregon's Willamette Valley was determined in response to glyphosate, tribenuron methyl (tribenuron), and fluazifop-p-butyl (fluazifop) herbicides. For glyphosate, Elymus trachycaulus, Festuca arundinacea, Madia elegans, Potentilla gracilis, and Ranunculus occidentalis were the most sensitive species, based on a concentration calculated to reduce shoot dry weight by 25% (IC25 values) of 0.02 to 0.04 × a field application rate of 1112 g active ingredient (a.i.) per hectare. Clarkia amoena and Lupinus albicaulis were the most tolerant to glyphosate, with IC25 values near the field application rate. Clarkia amoena, Prunella vulgaris, and R. occidentalis were the most sensitive to tribenuron, with IC25 values of 0.001 to 0.004 × a field application rate of 8.7 g a.i. ha(-1) for shoot dry weight. Five grass species were tolerant to tribenuron with no significant IC25 values. For fluazifop, 2 native grasses, E. trachycaulus and Danthonia californica, were the most sensitive species, with IC25 values of 0.007 and 0.010 × a field application rate of 210 g a.i. ha(-1) , respectively, for shoot dry weight, while a native grass, Festuca roemeri, and nearly all forbs showed little or no response. These results also indicated that the 3 introduced species used in the present study may be controlled with 1 of the tested herbicides: glyphosate (F. arundinacea), tribenuron (Leucanthemum vulgare), and fluazifop (Cynosurus echinatus).

Can we build better compost? Use of waste drywall to enhance plant growth on reclamation sites

Compost is a readily available source of organic matter and nutrients and is produced large scale in many jurisdictions. Novel advancements in composting include addition of construction waste, such as drywall, to address its disposal while potentially improving compost quality for use in land reclamation. Varying compositions (15–30% by weight) of coarse and ground waste drywall were added to manure and biosolids during composting. Six composts were applied at four rates (0, 50, 100, 200 Mg ha−1) to three reclamation soils (agricultural, urban clean fill, oil sands tailings). Response to composts was assessed in the greenhouse with three plant species (Hordeum vulgare L. (barley), Agropyron trachycaulum (Link) Malte (slender wheat grass) and Festuca saximontana Rydb. (rocky mountain fescue). Drywall added to biosolids or manure during composting had no detrimental effects on vegetation; any negative effects of compost occurred with and without drywall. In agricultural soil and clean fill, biosolids composts with 15% coarse and 18% ground drywall improved native grass response, particularly biomass, relative to biosolids compost without drywall. Drywall manure composts reduced native grass response relative to manure compost without drywall. Only low quality tailings sand was improved by 30% coarse drywall. Compost rate significantly affected above and below ground biomass in agricultural soil and reduced performance of native species at highest rates, suggesting a threshold beyond which conditions will not be suitable for reclamation. Grinding drywall did not significantly improve plant performance and use of coarse drywall would eliminate the need for specialized equipment and resources. This initial research demonstrates that drywall composts are appropriate soil amendments for establishment of native and non native plant species on reclamation sites with consideration of substrate properties and plant species tolerances to dictate which additional feed stocks should be used.

Predicting seed germination of slender wheatgrass [Elymus trachycaulus (Link) Gould subsp. trachycaulus] using thermal and hydro time models

Schellenberg, M. P., Biligetu, B. and Wei, Y. 2013. Predicting seed germination of slender wheatgrass [Elymus trachycaulus (Link) Gould subsp. trachycaulus] using thermal and hydro time models. Can. J. Plant Sci. 93: 793–798. Slender wheatgrass [Elymus trachycaulus (Link) Gould subsp. trachycaulus] is a native caespitose grass used for forage production and reclamation. The objective of this study was to quantify seed germination requirements of slender wheatgrass using thermal and hydro time models. Slender wheatgrass, San Luis, had a base temperature (Tb) of 9.48°C, and required 946.8°C h to reach 50% of seed germination. Seed germination of San Lius occurred at a temperature range of 10–30°C, with the highest germination rate being achieved at 20°C, and the highest final germination percentage being achieved at 25°C. At 20 and 25°C, San Luis had a hydro time constant of 61 MPa h, and a median base water potential of approximately 1.0 MPa, but the germination had low uniformity in reduced water potentials. Final germination was reduced at or lower than –0.6 MPa. Compared with many other cool-season native grasses of Northern Great Plains, a relatively warm temperature would be necessary for uniform seedling establishment of this grass. In reclamation seeding, the seedling emergence could reach the highest level at a temperature of 25°C.

Barley yield and nutrient uptake in rotation after perennial forages in the semiarid prairie region of Saskatchewan

Jefferson, P. G., Selles, F., Zentner, R. P., Lemke, R. and Muri, R. B. 2013. Barley yield and nutrient uptake in rotation after perennial forages in the semiarid prairie region of Saskatchewan. Can. J. Plant Sci. 93: 809–816. Alfalfa (Medicago sativa L.) is the most common perennial forage legume grown for hay and pasture in the semiarid Brown soil zone of the Canadian prairies. Perennial forages often are not recommended for inclusion in annual crop rotations due to lower grain yields and drier soils following forage stand termination, but this is based on research results from 50 yr ago. Three replicated experiments consisting of three grasses [slender wheatgrass (Elymus tracycaulus), intermediate wheatgrass (Elytrigia intermedia), and Dahurian wildrye (Elymus dahuricus)] grown in monoculture and in mixture with two alfalfa varieties (cv. Beaver or cv. Nitro) were terminated and seeded to barley (Hordeum vulgare ‘Harrington’) for 2 consecutive crop years at Swift Current, Saskatchewan. Soil water content was lower after the alfalfa–grass mixtures compared with the grass monocultures, even during a wet growing season. Barley yield and N concentration in the grain were significantly greater following Beaver alfalfa/grass mixture compared with grass monoculture in 3 and 4 of 6 site years, respectively. N uptake by the barley crop (grain and straw) was also significantly greater following Beaver alfalfa/grass mixture than following grass monoculture in all 6 yr. Both barley grain yield and N uptake after intermediate wheatgrass (grown in monoculture) were lower than after Dahurian wildrye or slender wheatgrass in 3 of 6 yr. The use of alfalfa and a short-lived grass species in hay and pasture mixtures in the Brown soil zone when grown in rotation with annual crops may indeed result in lower grain yields in the short term than continuous annual cropping systems, but the inclusion of alfalfa will provide a N benefit to the subsequent grain crop thereby enhancing yield and possibly its market value.

Stand Establishment and Persistence of Perennial Cool-Season Grasses in the Intermountain West and the Central and Northern Great Plains

The choice of plant materials is an important component of revegetation following disturbance. To determine the utility and effectiveness of various perennial grass species for revegetation on varied landscapes, a meta analysis was used to evaluate the stand establishment and persistence of 18 perennial cool-season grass species in 34 field studies in the Intermountain and Great Plains regions of the United States under monoculture conditions. Combined across the 34 studies, stand establishment values ranged from 79% to 43% and stand persistence values ranged from 70% to 0%. Intermediate wheatgrass (Thinopyrum intermedium [Host] Barkworth & D. R. Dewey), tall wheatgrass (Thinopyrum ponticum [Podp.] Z.-W. Liu & R.-C. Wang), crested wheatgrass (Agropyron spp.), Siberian wheatgrass (Agropyron fragile [Roth] P. Candargy), and meadow brome (Bromus riparius Rehmann) possessed the highest stand establishment (≥ 69%). There were no significant differences among the 12 species with the largest stand persistence values. Basin wildrye (Leymus cinereus (Scribn. & Merr.) Á. Löve), Altai wildrye (Leymus angustus [Trin.] Pilg.), slender wheatgrass (Elymus trachycaulus [Link] Gould ex Shinners), squirreltail (Elymus spp.), and Indian ricegrass (Achnatherum hymenoides [Roem. & Schult.] Barkworth) possessed lower stand persistence (≤ 32%) than the majority of the other species, and Indian ricegrass (0%) possessed the lowest stand persistence of any of the species. Correlations between environmental conditions and stand establishment and persistence showed mean annual study precipitation to have the most consistent, although moderate effect (r = ∼0.40) for establishment and persistence. This relationship was shown by the relatively poor stand establishment and persistence of most species at sites receiving less than 310mm of annual precipitation. These results will be a tool for land managers to make decisions concerning the importance of stand establishment, stand persistence, and annual precipitation for revegetation projects on disturbed sites.

Promising results in central Alberta with rough fescue (Festuca hallii) seeding following disturbance

Plains rough fescue (<i>Festuca hallii</i> (Vasey) Piper [Poaceae]), once dominant in rough fescue grasslands of central Canada and the northern US, recovers poorly if disturbed by cattle grazing or oil and gas production soil handling techniques. This long-lived bunchgrass requires 3 to 5 y to become established, during which time it may be exposed to competition from faster-growing species. Our first research objective was to assess the recovery of <i>F. hallii</i> by comparing a monoculture seeding to a mixed seeding of native grass (Poaceae) species including <i>F. hallii</i>, <i>Koeleria macrantha</i> (Ledeb.) Schult., <i>Elymus trachycaulus</i> (Link) Gould ex Shinners ssp. <i>subsecundus</i> (Link) Á. Löve &amp; D. Löve, <i>Pascopyrum smithii</i> (Rydb.) Á. Löve, <i>Nassella viridula</i> (Trin.) Barkworth, <i>Hesperostipa comata</i> (Trin. &amp; Rupr.) Barkworth, and <i>Bouteloua gracilis</i> (Willd. ex Kunth) Lag. ex Griffiths. Our second objective was to evaluate plant community development of both seeding mixes. We established seeding experiments on 3 field sites in central Alberta, Canada. When seeded as a monoculture, the <i>F. hallii</i> plant community that resulted included native species re-established from the seedbank or seed rain, such as <i>Helictotrichon hookeri</i> (Scribn.) Henr. (Poaceae). In the native species seed mix, wheatgrasses (<i>E. trachycaulus</i> ssp. <i>subsecundus</i> and <i>P. smithii</i>) dominated in the first 3 y with low <i>F. hallii</i> cover. We recommend seeding <i>F. hallii</i> with few or no aggressive species, such as wheatgrasses, in the seed mix.

Short- and Long-Term Effects of Modified Humic Substances on Soil Evolution and Plant Growth in Gold Mine Tailings

Mining creates large amounts of processed waste in the form of mine tailings. Sulfide mine tailings are of particular concern due to the biotic and abiotic oxidation of sulfide minerals that release acidity and metals into the environment. Revegetation can be employed to mitigate the spread of tailings in the environment. Revegetation often involves ameliorating tailings with organic materials to promote plant growth and improve tailings physicochemical structure. We amended plots in the Central Manitoba Mine tailings pond with humic substances applied at rates up to 4 g C kg−1 through roto-tilling and seeded with Medicago sativa and Elymus trachycaulus in 2003 and 2004. The humic substances improved tailings fertility by increasing macro aggregation, organic carbon, and macronutrients but also resulted in a short-term increase in electrical conductivity levels. In the first growing season the humic amendment had little effect on plant yield, except in the 2003 experiment where the yield of E. trachycaulus decreased by 84 % with 4 g C kg−1 amendment. After 7 years, the addition of humic amendment resulted in a cover of over 38 % for M. sativa, compared to less than 2 % in control plots. In addition, non-seeded species cover increased with amendment rate in the 2003 experiment but not the 2004 experiment, most likely due to lower pH in the latter. Our results suggest that short-term patterns of plant performance do not reflect longer-term performance or invasion by volunteer plant species. Our long-term data suggest that humic amendments can be effective in establishing plant invasion of mine tailings, although the effects vary depending on the pH of the tailings.

Effectivity of arbuscular mycorrhizal fungi collected from reclaimed mine soil and tallgrass prairie

We examined suitability of arbuscular mycorrhizal fungi (AMF) associated with cool-season nonnative forages on reclaimed surface-mined land in southeast Ohio for establishment of native warm-season grasses. The goal of establishing these grasses is to diversify a post-reclamation landscape that is incapable of supporting native forest species. A 16-week glasshouse study compared AMF from a 30-year reclaimed mine soil (WL) with AMF from native Ohio tallgrass prairie soil (CL). Four native grasses were examined from seedling through 16 weeks of growth. Comparisons were made between CL and WL AMF on colonized (+AMF) and non-colonized plants (–AMF) at three levels of soil phosphorus (P). Leaves were counted at 4 week intervals. Shoot and root biomass and percent AMF root colonization were measured at termination. We found no difference between WL and CL AMF. Added soil P did not reduce AMF colonization, but did reduce AMF efficacy. Big bluestem (Andropogon gerardii Vitman), Indiangrass (Sorghastrum nutans (L.) Nash), and tall dropseed (Sporobolus asper (Michx.) Kunth) benefited from AMF only at low soil P while slender wheatgrass (Elymus trachycaulus (Link) Gould ex Shinners) exhibited no benefit. Establishment of tallgrass prairie dominants big blue-stem and Indiangrass would be supported by the mine soil AMF. It appears that the non-native forage species have supported AMF equally functional as AMF from a regionally native tallgrass prairie. Tall dropseed and slender wheatgrass were found to be less dependent on AMF than big bluestem or Indiangrass and thus would be useful in areas with little or no AMF inoculum.

Species dynamic, forage yield, and nutritive value of seeded native plant mixtures following grazing

Schellenberg, M. P, Biligetu, B. and Iwaasa, A. D. 2012. Species dynamic, forage yield, and nutritive value of seeded native plant mixtures following grazing. Can. J. Plant Sci. 92: 699–706. There is increasing interest in native plants of North America for rangeland reseeding in the semiarid regions of western Canada. However, there is limited information available on forage yield, nutritive value and response to grazing of seeded native plant mixture. The objective of this study was to compare foliar cover, forage yield, and nutritive value of two different native plant mixtures under grazing. In 2001–2004, a study was initiated on Orthic Brown Chernozemic (Aridic Haploboroll) soil near Swift Current (lat. 50°25'N, long. 107°44'W), Canada. The experimental design was a randomized complete block with a full factorial arrangement of seed mixtures (7 species and 14 species) and stocking rates (1.3 and 2.7 animal units ha−1) with four replications of each treatment combination. The 7-species mixture produced more forage than the 14-species mixture in July, August, and September of all study years. The 7-species mixture, however, had lower crude protein than the 14-species mixture in August and September in 2002 and 2003. Neutral detergent fiber and acid detergent fiber concentrations were generally similar between the two mixtures for July, August, and September sampling for all 3 yr. Foliar cover of awned wheatgrass [(Elymus trachycaulus (Link) Gould &amp; Shin. ssp subsecundus (Link) A. &amp; D. Love.] and slender wheatgrass [Elymus trachycaulus (Link) Gould subsp. trachycaulus] was higher in the 7-species mixture than the 14-species mixture, but foliar cover of other species was similar between the two mixtures. Shrub species were rarely observed in the stand at any year. Higher stocking rate decreased foliar cover of slender wheatgrass and weeds, but foliar cover of other species was similar between the two stocking rates. Based on the results, a seed mixture of native cool-season grasses may be more productive than a combination of native warm- and cool-season grasses under semiarid region of western Canada, but inclusion of warm-season grasses could improve nutritive value by increasing protein content in the late summer months.

Bacterial endophyte communities of two wheatgrass varieties following propagation in different growing media

Bacterial endophyte communities of two wheatgrass varieties currently being used in the revegetation of military training ranges were studied. Culturable and direct 16S rDNA PCR amplification techniques were used to describe bacterial communities present in Siberian and slender wheatgrass seeds, leaf tissues, and root tissues following propagation in either sand or a peat-based growing mix. Our hypothesis was that the resulting plant endophytic communities would be distinct, showing not only the presence of endophytes originating from the seed but also the characteristics of growth in the two different growing media. Both culture and culture-independent assays showed the likely translocation of Actinobacteria, Firmicutes, and Gammaproteobacteria from seed to mature plant tissues as well as subsequent colonization by exogenous organisms. Statistical analysis of 16S terminal restriction fragment profiles identified growing media as having a greater significant effect on the formation of the endpoint endophytic communities than either plant tissue or wheatgrass variety. In silico digests of the ribosomal database produced putative identifications indicating an increase in overall species diversity and increased relative abundances of Firmicutes and Cyanobacteria following propagation in sand and Betaproteobacteria following propagation in the peat-based growing mix. Results indicated a substantial translocation of endophytes from seed to mature plant tissues for both growing media and that growing medium was a dominant determinant of the final taxonomy of the endpoint plant endophytic communities.

Enhancing predation of a subterranean insect pest: A conservation benefit of winter vegetation in agroecosystems

Generalist predator communities are abundant and diverse in agroecosystems, but pests often persist nevertheless. Winter vegetation (e.g., cover crops) provides an agronomically sound opportunity to conserve predator communities and promote their impact on pests. We evaluate whether winter vegetation increases predation of Diabrotica virgifera, a key subterranean pest of maize. Fields of maize were preceded by a winter cover crop (slender wheatgrass) or a fallow period (bare soil) over two years. Pest populations and root damage were measured in each field, from which the gut contents of predators aspirated from the soil surface, or extracted from the soil column, were analyzed using qPCR and primer sets specific to D. virgifera COI gene sequences. Predation intensity on restrained D. virgifera larvae (sentinels) was observed during the three larval stadia of the pest ( n = 400 3rd instars per plot per stadium). A diverse predator community consumed D. virgifera in maize fields, and predation was significantly greater in maize following cover crops (as measured with sentinels, but not gut content analysis). Predation was particularly intense during the 3rd stadium of the pest, especially in the cover-cropped maize. qPCR-based gut content analysis of natural populations functioned well in determining which predators consumed D. virgifera, but was only correlated with their impact on the pest and its damage when the relative frequency of detection, quantity of DNA calculated, and predator abundance were combined into a predation index. In support of these observations, predation intensity on sentinels was negatively correlated with D. virgifera populations and plant damage, but did not provide an accurate picture of the community involved. Cover crops reduced D. virgifera populations by increasing predation levels on this pest, which indicates that conserving predation as an ecosystem service is a mechanism for how this form of habitat diversification functions. Also, we conclude that employing diverse methods provides the best insight into trophic relationships within subterranean systems. Finally, because of the dynamic and diverse interactions between pests and their natural enemy complexes, we advocate conserving diverse predator communities within agroecosystems, rather than targeting conservation efforts at specific key predator taxa.

Defoliation Effects on Herbage Production and Root Growth of Wet Meadow Forage Species

Abstract Root growth is important to the competitive ability of plants, and understanding how herbage defoliation affects root growth has implications for development of management strategies. Objectives were to determine the effects of defoliation intensity and frequency on root characteristics and herbage production of slender wheatgrass ( Elymus trachycaulus [Link.] Shinners), Nebraska sedge ( Carex nebrascensis C. Dewey), and “Steadfast” birdsfoot trefoil ( Lotus corniculatus L.). Plants of each species were transplanted into containers that had been placed in the ground at wet meadow field sites the prior year. There were eight replications of a control and five defoliation treatments, which were combinations of different frequencies (two or five times) and intensities (light or heavy) and haying. Treatments were applied for a single growing season, and aboveground biomass was collected. Containers were extracted in October, and plant crowns, rhizomes, and roots were separated from the soil. Defoliation treatment did not affect total root weight, length, and surface area of Nebraska sedge or birdsfoot trefoil ( P >0.10). Slender wheatgrass total root weight was less when defoliated five times (4.46 g·container −1 ) than when defoliated twice (6.62 g·container −1 ) during the growing season. More frequent defoliation of slender wheatgrass also reduced length (20%) and surface area (21%) compared to less frequent defoliation. However, defoliation frequency did not affect aboveground biomass. Defoliation intensity did not affect aboveground production or root characteristics of the three species. Abundant soil moisture in meadows likely buffers negative effects of defoliation. For all species, two defoliation events (e.g., haying followed by grazing) does not appear to negatively affect root growth and herbage production.

Russian wheat aphid (Hemiptera: Aphididae) reproduction and development on five noncultivated grass hosts

The Russian wheat aphid, Diuraphis noxia (Kurdjumov), is a small grains pest of worldwide economic importance. The Russian wheat aphid is polyphagous and may encounter differential selective pressures from noncultivated grass hosts. Aphid biotypic diversity can disrupt the progress of plant breeding programs, leading to a decreased ability to manage this pest. The goal of this research was to quantify Russian wheat aphid biotype 2 (RWA2) reproductive and development rates on five common noncultivated grass hosts to gain information about host quality, potential refuges, and sources of selection pressure. First, RWA2 reproduction was compared on crested wheatgrass (Agropyron cristatum, (L.) Gaertn.), intermediate wheatgrass (Elytrigia intermedia, (Host) Nevski), slender wheatgrass (Elymus trachycaulus, (Link) Gould ex Shinners), western wheatgrass (Pascopyrum smithi, (Rydb.) A. Love), and foxtail barley (Hordeum jubatum, (L.) Tesky) at 18–24°C. Second, RWA2 reproduction was compared on intermediate and crested wheatgrass at three temperature regimes 13–18°C, 18–24°C, and 24–29°C. At moderate temperatures (18–24°C), the intrinsic rate of increase values for all five hosts ranged from 0.141 to 0.199, indicating the possibility for strong population sources on all tested hosts. Aphids feeding on crested and intermediate wheatgrass at the 13–18°C temperature had lower fecundity, less nymph production days, longer generational times, and lower intrinsic rate of increase than aphids feeding at the 18–24°C temperature regime. Aphids feeding at 24–29°C did not survive long enough to reproduce. The positive intrinsic rates of increase in Russian wheat aphid on the wheatgrasses suggest that these grasses can support aphid populations at moderate to low temperatures.

Phosphogypsum Capping Depth Affects Revegetation and Hydrology in Western Canada

Phosphogypsum (PG), a byproduct of phosphate fertilizer manufacturing, is commonly stacked and capped with soil at decommissioning. Shallow (0, 8, 15, and 30 cm) and thick (46 and 91 cm) sandy loam caps on a PG stack near Fort Saskatchewan, Alberta, Canada, were studied in relation to vegetation establishment and hydrologic properties. Plant response was evaluated over two growing seasons for redtop ( L.), slender wheatgrass ( (Link) Malte ex H.F. Lewis), tufted hairgrass ( (L.) P. Beauv.), and sheep fescue ( L.) and for a mix of these grasses with alsike clover ( L.). Water content below the soil-PG interface was monitored with time-domain reflectometry probes, and leachate water quantity and quality at a depth of 30 cm was measured using lysimeters. Vegetation responded positively to all cap depths relative to bare PG, with few significant differences among cap depths. Slender wheatgrass performed best, and tufted hairgrass performed poorly. Soil caps <1 m required by regulation were sufficient for early revegetation. Soil water fluctuated more in shallow than in thick caps, and water content was generally between field capacity and wilting point regardless of cap depth. Water quality was not affected by cap depths ≤30 cm. Leachate volumes at 30 cm from distinct rainfall events were independent of precipitation amount and cap depth. The study period had lower precipitation than normal, yet soil caps were hospitable for plant growth in the first 2 yr of establishment.

The Effects of a Winter Cover Crop on Diabrotica virgifera (Coleoptera: Chrysomelidae) Populations and Beneficial Arthropod Communities in No-Till Maize

The effects of an autumn-planted, spring-killed, grass cover crop (Elymus trachycaulus [Link] Gould ex Shinners) on populations of Diabrotica virgifera virgifera LeConte and its predator community were evaluated in South Dakota maize fields over two seasons. Abundance and size of D. virgifera larvae and adults and sex ratio of adults were measured in maize produced under two treatments (i.e., a winter cover crop or bare soil), as were maize root damage and the abundance and diversity of the predator communities collected on the soil surface and in the soil column. First and second instars and adults of D. virgifera were similarly abundant in the two treatments, but third instars were significantly fewer in maize planted after a winter cover crop. Larvae developed at different rates in the two treatments, and second instars were significantly smaller (head capsule width and body length) in the maize planted after a cover crop. First and third instars and adults were of similar size in the two treatments, and adult sex ratios were also similar. Although initially similar, predator populations increased steadily in the cover-cropped maize, which led to a significantly greater predator population by the time D. virgifera pupated. There was significantly less root damage in the cover-cropped maize. Predator communities were similarly diverse in both treatments. Predator abundance per plot was significantly and negatively correlated with the abundance of third instars per plot. Clearly, winter cover crops reduce D. virgifera performance and their damage to the crop, and we suspect that this reduction is caused by both environmental effects of the treatment on D. virgifera size and development, and of increased predation on the third instars of the pest. Additional data on the impact of cover crops on actual predation levels, grain yield and quality, and farmer profitability, and correlations among pest performance, crop characteristics, and predator populations and behaviors are key components of this system that remain to be addressed.

Early Wisconsinan (MIS 4) Arctic ground squirrel middens and a squirrel-eye-view of the mammoth-steppe

Fossil arctic ground squirrel ( Spermophilus parryii) middens were recovered from ice-rich loess sediments in association with Sheep Creek-Klondike and Dominion Creek tephras (ca 80 ka) exposed in west-central Yukon. These middens provide plant and insect macrofossil evidence for a steppe-tundra ecosystem during the Early Wisconsinan (MIS 4) glacial interval. Midden plant and insect macrofossil data are compared with those previously published for Late Wisconsinan middens dating to ∼25–29 14C ka BP (MIS 3/2) from the region. Although multivariate statistical comparisons suggest differences between the relative abundances of plant macrofossils, the co-occurrence of steppe-tundra plants and insects (e.g., Elymus trachycaulus, Kobresia myosuroides, Artemisia frigida, Phlox hoodii, Connatichela artemisiae) provides evidence for successive reestablishment of the zonal steppe-tundra habitats during cold stages of the Late Pleistocene. Arctic ground squirrels were well adapted to the cold, arid climates, steppe-tundra vegetation and well-drained loessal soils that characterize cold stages of Late Pleistocene Beringia. These glacial conditions enabled arctic ground squirrel populations to expand their range to the interior regions of Alaska and Yukon, including the Klondike, where they are absent today. Arctic ground squirrels have endured numerous Quaternary climate oscillations by retracting populations to disjunct “interglacial refugia” during warm interglacial periods (e.g., south-facing steppe slopes, well-drained arctic and alpine tundra areas) and expanding their distribution across the mammoth-steppe biome during cold, arid glacial intervals.

Effects of plant competition, seed predation, and nutrient limitation on seedling survivorship of spotted knapweed (Centaurea stoebe)

We measured seed germination and seed- ling survivorship of spotted knapweed, Centaurea stoebe, in a series of laboratory and field experiments to evaluate the efficacy of seed limitation as a management focus. This work was initiated 6 years after introduction of several biological control agents. The soil seed bank of the site used in this study contained a mean density of 5,848 seeds/m 2 (ranging from 0 to 16,364 seeds/m 2 ), and 92% of the seeds isolated from soils were shriveled, discolored, and/or partially decayed. Additionally, none of the intact seeds germinated, suggesting that the viable seed bank at our field study site has been exhausted. Centaurea stoebe seeds were planted into pots under a range of soil nitrogen (N) availability, with half of the pots containing a single density of previously established seedlings of a native cool-season grass, slender wheatgrass (Elymus trachycaulus). A watering regime mimicking local precipitation was applied. Spotted knapweed exhibited large biomass responses to N addition, but the presence of grasses suppressed the ability to exploit this N. Surprisingly, low soil N conditions improved knapweed survivorship in the presence of grasses. Nevertheless, recruitment and biomass were still far below the levels reached in the absence of competition. To evaluate the effect of density on successful recruitment, Centaurea stoebe seed was introduced into a meadow at three densities matching reduced levels of seed production under the constraints of seed predators. These densities were sown with or without a seed mixture of native species, into an existing plant community lacking C. stoebe, and seedling recruitment was recorded over 2.5 years. Across all plots and densities sown (568-2,272 seeds m -2 year -1 ), seedling recruitment was less than 1%. The invasion potential of spotted knapweed was greatly diminished when realistic levels of plant competition and biological control limit seed production. We therefore conclude that a com- bination of seed limitation and shortage of 'safe sites' within undisturbed vegetation can limit densities of C. stoebe.

English

The Stucky Ridge test plot is located approximately two miles northeast of Anaconda, Montana. Sparse vegetation includes quaking aspen, Wood's rose, currant species, rubber rabbitbrush, horsebrush, redtop and basin wildrye. In November 2002, 22 tons/acre lime kiln dust was disced to a 12‖ depth. Prior to spring seeding, 500 bulk-pounds of fertilizer were applied/incorporated to a 6‖ depth. Thirty-six grass, two subshrub accessions, and four seed mixtures were arranged in a Randomized Complete Block with four replications. Plots were drill-seeded in May 2003 at 50 Pure Live Seeds per linear row foot. Soil sampling for arsenic and heavy metals occurred after planting. A significant amount of new grass seedling emergence was detected during the June 2004 evaluation, particularly for Indian ricegrass, western wheatgrass, Nevada bluegrass, and basin wildrye. Following the 2004, 2005, and 2006 evaluations for cover and vigor rating, each plot was sampled for biomass production. Tissue analyses indicated heavy metal concentrations were generally within limits for livestock and wildlife use. In single-species plots, ‗local source' plants exhibiting superior performance included Copperhead and 9081621 slender wheatgrass, Opportunity Nevada bluegrass, 9081968 western wheatgrass, 9081624 and Washoe Germplasm basin wildrye, 9081628 Indian ricegrass, 9081636 bluebunch wheatgrass and 9081635 Canbyi bluegrass. In 2003, Open Range Germplasm winterfat was the only broad-leaved entry demonstrating significant emergence. In 2007, the only broad-leaved plants surviving were Open Range winterfat and 9081632 silverleaf phacelia. Old Works fuzzytongue penstemon and silverleaf phacelia were only detected in alternating years. In the Seed Mixture Trials, the ‗Experimental' mixes containing native ‗local source' were far superior to the ‗Developed' mixes consisting of native ‗nonlocal source' (Upland Mix) and introduced cultivars (Waste Management Areas). However, it was estimated the majority of plants in the Experimental mixtures were Copperhead slender wheatgrass, the best overall performer on the test site.