Slender Setae Research Articles

A new deep-sea Cristaphyes (Kinorhyncha: Allomalorhagida: Pycnophyidae) from the continental rise of South Island, New Zealand

ABSTRACT A new kinorhynch species, Cristaphyes microtubuliferus sp. nov., is described from the continental rise of South Island, New Zealand. The new species is recognised by the presence of very poorly developed lateral terminal spines, combined with its considerable trunk length, which can exceed 1 mm. In addition, the new species is characterised by morphologically dimorphic setae, i.e. the regular long and slender setae found in most pycnophyid species, and much shorter and more rigid ‘minute setae’. Besides the new species, the presence of two additional undescribed Cristaphyes species is reported. The material is insufficient to support formal species descriptions for the latter two, but short diagnoses are provided for the species. The description of Cristaphyes microtubuliferus sp. nov. brings the total number of described and identified kinorhynch species in New Zealand up to 19. With the new species included, the genus Cristaphyes now accommodates 26 species, of which only six are known from the southern hemisphere. LSID: urn:lsid:zoobank.org:pub: 77FEA91A-7684-4664-9DFC-470A245D9267

Two new species of Sarothrogammarus (Crustacea, Amphipoda) from Swat Valley, Pakistan

Two new species of the genus Sarothrogammarus Martynov, 1935 are described and illustrated from Pakistan. Sarothrogammarus miandamensis sp. nov. is characterized by the oval eyes; pereopod III having filtrative setae on merus, carpus and propodus; uropod III terminal article distinct and inner ramus scale-shaped; and having robust setae and slender setae on urosomites I–III. Sarothrogammarus kalamensis sp. nov. can be distinguished from the above species by pereopod III having filtrative setae on merus, but few on carpus and propodus. Detailed morphological characteristics, as well as comparisons with closely related species are provided. Molecular phylogenetic analyses suggest that S. miandamensis sp. nov. is clustered with S. kalamensis sp. nov., with a genetic distance of 19% for COI between the two species.

A new species of the genus Burmadactylus Heads, 2009 from mid-Cretaceous amber in north Myanmar (Orthoptera: Caelifera: Tridactyloidea).

This paper describes a new species of the genus Burmadactylus Heads, 2009 (Tridactylidae: Dentridactylinae) from Burmese amber, namely: Burmadactylus tenuicerci sp. nov. This new species is similar to Burmadactylus grimaldi Heads, 2009, but differs from latter by mesotibia basally inflated and almost as long as mesofemur; the second segment of cercus distinctly slender; paraproctal lobe covered with sparse and slender setae, one of the setae near the apex distinct thick and long.

REPORT OF A BRYOPHYTIC CAPSULE FROM THE DECCAN INTERTRAPPEAN SERIES OF MADHYA PRADESH, INDIA.

A sporophyte measure 5.5 mm long and shows a short, slender seta and an oval erect, capsule. A smooth, hood shaped calyptra covers the top. It contains ten spore sacs (spore chambers) in longitudinal section. The spore sacs measures are 1.7 mm long and 300 to 500 µm in diameter are separated by parenchymatous septae. The apophysis is poorly differentiated. The operculum is seen as an outline. Columella and peristomial teeth are lacking. Spore and spores tetrad are surrounded by pseudo-elaters. The spores are meiogenous, arranged in tetrahedral tetrads. The seta is short and slender. The calyptra is hood-shaped. As it shows close similarities with Krempogonium mohgaonensis (Nambudiri, Chitaley & Yawale, 2004) it is kept under the genus Krempogonium as a new species Krempogonium deccanii sp.nov.

К систематике водяного клеща Feltria ishikariensis Imamura (Acari, Hydrachnidia, Feltriidae)

First illustrated description of male and redescription of female of the water mite Feltria ishikariensis Imamura, 1954 from running waters of Primorye Territory of Russia is given. Dorsum of male with large shield and two narrow posterior platelets. Dorsal shield bearing four pairs of setae, posterior plates with one glandularia each. Coxal plates in four groups. The genital field with deep posteromedian indentation, 40–50 pairs of acetabula and four pairs fine medial setae. III-Leg-6 with short ventrodistal projection bearing four to five slender setae. Excretory pore in posteroventral position.

Paramisophria walteri a new Arietellidae (Copepoda, Calanoida) from Midway Island

ABSTRACT A new species of the genus Paramisophria T. Scott 1897 (Copepoda: Calanoida) was collected from coastal waters off Midway Island. Paramisophria walteri sp. nov., along with Paramisophria variabilis, Paramisophria platysoma and Paramisophria bathyalis, are the only representatives of the genus sharing four armature elements on the third exopod article of the male left leg 5. The new species can be easily distinguished from P. variabilis by differences in the armature elements on third exopod article of the male left leg 5 (terminal spine pinnate, plus three subequal spines, compared with terminal spine naked, plus two subequal spines and one short process in P. variabilis). The new species can be easily distinguished from P. platysoma by its symmetrical prosome (prosome asymmetrical in P. platysoma), and the endopodal lobes of the female leg 5 each bearing one long plumose seta (setae are lacking in P. platysoma). Significant differences between the new species and P. bathyalis include the 4 + 1 armature elements of the maxillulary praecoxal arthrite (5 + 1 in P. bathyalis); and the third exopod article of the male right leg 5 with three distal stout spines, compared with one slender inner seta plus two thin subequal outer setae in P. bathyalis. Phylogenetic relationships within the genus Paramisophria are re-examined and discussed.

A new species of Sunamphitoe Bate, 1857 (Crustacea: Amphipoda: Ampithoidae) from Hokkaido, Japan

AbstractA new species of ampithoid amphipod, Sunamphitoe gigantea sp. nov., is described from Katsurakoi, Hokkaido, Japan. The new species most closely resembles S. eoa, but can be distinguished from it by the shorter flagellum of antenna 1, the maxilla 1 inner plate that bears two slender setae, the short dactylus of male gnathopod 2, and the presence of a group of long setae on anterior margins of pereopods 3 and 4 bases. Nucleotide sequences of mitochondrial cytochrome c oxidase subunit I obtained from the type specimens also differentiated this new species from the congeners. Ontogenetic morphological changes of the male gnathopod 2 are briefly discussed.

Eukoenenia igrejinha (Palpigradi: Eukoeneniidae), a new cave-dwelling palpigrade from southeastern Brazil

Eukoenenia igrejinha sp. nov. is described on the basis of one male and one female from the cave Gruta da Igrejinha, state of Minas Gerais, southeastern Brazil. The new species exhibits morphological features unique among the South American species of Eukoenenia described to date, such as a single pair of paramedian thickened setae flanked by one slender seta on each side of opisthosomal sternites IV–VI and 9 pairs of setae on the first genital lobe of the female. Additionally, we provide the coxal formulae of the South American species E. maquinensis, E. spelunca, E. improvisa and E. janetscheki.

Pseudolella tenuis sp. n. and Paracomesoma leptum sp. n. (Nematoda, Araeolaimida) from Vietnam

Illustrated descriptions of two new nematode species found in artificial reservoirs for shrimp cultivation are given: Pseudolella tenuis sp. n. and Paracomesomaleptum sp. n. The former is morphologically close to P. bengalensis (Timm 1957) and P. capera Tchesunov 1978, but differs from P. bengalensis by a shorter body, comparatively longer pharynx, shorter and less slender tail, longer stoma, shorter ventral branch of the amphideal fovea, and shorter spicules and cephalic setae. It differs from P. capera by shorter cephalic setae, wider labial region, longer ventral branch of the amphideal fovea, and longer spicules. Paracomesomaleptum sp. n. is morphologically close to P. susannae Semprucci 2015 and P. minor Gagarin et Nguyen Dinh Tu 2014. From the former species it differs by its shorter and thicker body, shorter cephalic and subcephalic setae, and less numerous precloacal supplements in the male. This new species differs from P. minor by a less slender tail, shorter cephalic and outer labial setae, the presence of subcephalic setae, and longer spicules.

New material of shore bug subfamily Leptosaldinae in mid-Cretaceous Kachin amber from northern Myanmar (Heteroptera, Leptopodomorpha)

The shore bug subfamily Leptosaldinae is a relict insect group with only one living species recorded in Ecuador and Colombia, but its palaeo-diversity is relatively high, especially in upper Mesozoic amber biotae. Herein, on the basis of an adult shore bug fossil trapped in a mid-Cretaceous Kachin amber piece from northern Myanmar, we describe a new genus and species Cretaleptus popovi gen. et sp. nov., attributed to Leptosaldinae. The new taxon differs from all known living and extinct leptosaldines by possessing a series of unique morphological traits: the head greatly porrect, antennae densely covered with short and slender setae but very long setae absent, with segment II slightly shorter than III and the latter slightly shorter than IV, the pro- and mesonotum tinged with longitudinal stripe in midline, hemelytra darkly pigmented with two distinct light stripes and two speckles, the corium and clavus with three rows of punctures respectively, and membrane with four closed cells. In this study, a key to all leptosaldine genera is also provided. Our find further confirms that this enigmatic leptopodomorph lineage was likely abundant and with high diversity in the late Mesozoic.

The psyllid genus Pseudophacopteron (Hemiptera: Psylloidea) from China

The Chinese fauna represents a small portion of the species diversity of the psyllid genus Pseudophacopteron (Phacopteronidae) of the world. Here, Pseudophacopteron from China is revised, and four species are recognized. Pseudophacopteron quinquipannis sp. nov. is described; Pseudophacopteron alstonium is synonymized with Pseudophacopteron tuberculatum; Pseudophacopteron album, Pseudophacopteron aphanamixis and P. tuberculatum are redescribed. Comparison is made between the four species and Phacopteron. The latter possesses unsplit fields of radular spinules in cells m1, m2 and cu1 of the fore wing, and thick tibiae covered by dense long and slender setae, which are different from Pseudophacopteron.http://www.zoobank.org/urn:lsid:zoobank.org:pub:08B185EB-462F-46CF-B4CB-B733D1E1A864

Pseudorchomene boreoplebs, a new lysianassid amphipod from Korean waters (Crustacea, Amphipoda, Lysianassoidea)

Until now, the genusPseudorchomeneSchellenberg, 1926 has been recorded exclusively in Antarctic or sub-Antarctic regions.Pseudorchomeneboreoplebssp. n., described from Korean waters, is the first record from the Northern Hemisphere as well as from temperate regions. This new species is very similar toPseudorchomeneplebs(Hurley, 1965), but can be readily distinguished by its wider basis of gnathopod 1, elongate carpus and angulate anterodistal corner of the propodus on gnathopod 2, having slender setae only on the posterior margin of the carpus on pereopod 5, and lacking posterior lobations of the carpus on pereopods 5–7.

A new species of Nuuanu (Crustacea, Amphipoda, Nuuanuidae) from a Caribbean coral reef with identification keys to males and females of Nuuanu species

A new species of amphipod of the genus Nuuanu is described and illustrated, and is diagnosed against the morphologically most similar species. It was collected in coral rubble from a tropical coral reef, NW Caribbean Sea. The new species increases the number of Nuuanu species recorded to 20 worldwide. Nuuanu jaumei sp. nov., can be distinguished from other members of the genus by a combination of characters: head with bigeminal dispersed eyes; mammiliform lateral cephalic lobe; anteroventral notch of head well defined; antenna 1 geniculate; accessory flagellum 3–articulate; coxa 5 devoid of seta on ventromedial margin; article 2 anteroventral corner of pereopod 5 with a strong setae; epimera 1, 2, 3 with 2, 4, 1 ventral robust setae respectively; telson longer than broad; female gnathopod 2 with 2 long and slender setae on posterior margin. In addition, a key for the identification of all known male species of the genus Nuuanu, and another for the identification of the females, are also presented.

A molecular phylogeny of Pseudocrangonyx from Japan, including a new subterranean species (Crustacea, Amphipoda, Pseudocrangonyctidae)

A subterranean species of pseudocrangonyctid amphipod, Pseudocrangonyx gudariensis Tomikawa & Sato, sp. n., is described from the spring-fed stream Gudari-numa in Hakkoda Mountains, Aomori Prefecture, northern Japan. Pseudocrangonyx gudariensis is morphologically similar to P. coreanus Uéno, 1966 and P. febras Sidorov, 2009 based on its relatively small body size, small number of articles of rami of pleopods, and urosomite 1 without basal setae. However, P. gudariensis is distinguished from those species based on the following characteristics: from P. coreanus, antenna 2 of female without calceoli, palmar margins of gnathopods 1 and 2 with distally notched robust setae, inner margin of inner ramus of uropod 2 with 4 robust setae, and basal part of inner ramus of uropod 2 without slender seta; and from P. febras, carpus of gnathopod 2 without serrate robust setae on posterodistal corners, peduncle of pleopods 1 and 2 with setae, and longer article 2 of uropod 3. Phylogenetic analyses using nuclear 28S rRNA and histone H3, and mitochondrial cytochrome c oxidase subunit I and 16S rRNA markers showed that P. gudariensis is placed among known Pseudocrangonyx Akatsuka and Komai, 1922 species. However, its exact phylogenetic position within the genus could not be determined. The polyphyly of the Japanese Pseudocrangonyx species indicates that multiple colonization events of Pseudocrangonyx ancestors to the Japanese Archipelago could have occurred. The reliability of the past Pseudocrangonyx records from Japan is briefly discussed.

A new species of Speleophriopsis (Copepoda: Misophrioida) from an anchialine cave in the Adriatic Sea, Mediterranean

A new copepod species, Speleophriopsis mljetensis, collected from an anchialine cave Bjejajka on Mljet Island (Croatia) is described. The prosome of the new species is 5-segmented; the urosome has five somites in females and six in males; the genital double-somite is symmetrical and is longer than wide; the caudal rami are symmetrical, with seta I well developed; the antennules of both sexes are 27-segmented and symmetrical; the antennal exopod is 7-segmented; the maxillule possesses 15 armature elements on the praecoxal arthrite and the proximal basal endite has three slender setae and one stout claw-like seta, the fifth legs are symmetrical, 4-segmented and uniramous, and the distal segment is elongate and armed with seven and six elements in males and females, respectively. Speleophriopsis mljetensis is comparated with similar species S. balearicus and S. canariensis. The new species is mainly distinguished by the armature of the genital operculum, the fifth legs of both sexes, maxilliped distal segment armature and total length of both sexes. This is the second report of a speleophriid copepod, otherwise thought to be a Tethyan relict, from an Adriatic anchialine cave. In the Bjejajka Cave, the salinity varied between 1.5 at the surface to 39 psu at the bottom, and the temperature ranged between 14.2 and 16.6 °C. It might be assumed that it colonised Bjejajka anchialine cave from its natural deep-sea habitat. However, the distributions of speleophriids in the Mediterranean are difficult to explain; therefore, this finding is of particular importance, contributing to our knowledge of the global biogeography of these Tethyan relicts.

Spirobolbolaimus undulatussp. nov. in intertidal sediment from the East China Sea, with transfer of twoMicrolaimusspecies toMolgolaimus(Nematoda, Desmodorida)

Spirobolbolaimus undulatussp. nov. is described in intertidal sandy sediment from the East China Sea. The new species is characterized by six jointed outer labial setae much thicker and longer than the four slender cephalic setae, anterior and posterior pharynx bulbs, sclerotized multispiral amphid fovea, eight longitudinal rows of postamphideal setae, gubernaculum apophysis and many precloacal supplements. The new species differs distinctly from the type speciesSpirobolbolaimus bathyalisin the presence (vsabsence) of jointed outer labial setae, eight additional small teeth, gubernaculum apophysis and many precloacal supplements and eight (vssix) rows of postamphideal setae. It differs from the other known congenerSpirobolbolaimus boucherorumin the jointed outer labial setae (vssetae with smooth outline), three main teeth with eight additional small teeth (vsonly three main teeth) and many distinct precloacal supplements (vsseven tiny papillate ones). We emend the diagnosis of the genusSpirobolbolaimusbased on the description.Microlaimus pecticaudaandMicrolaimus spiriferpossess two opposed reflexed ovaries, and thus are transferred from the family Microlaimidae toMolgolaimusof the family Desmodoridae:Molgolaimus pecticaudacomb. nov. andMolgolaimus spirifercomb. nov.

A new species of Atergopedia (Copepoda: Harpacticoida: Novocriniidae) from a Caribbean mesophotic reef

Coordinated research efforts in mesophotic coral reefs are only a decade old, but have already produced exciting findings in ecology, physiology, geomorphology, population genetics, biodiversity, and taxonomy. In this taxonomic contribution, we describe both sexes of a new copepod species of the family Novocriniidae Huys and Iliffe in Zool Scr 27(1):1–15, 1998 from a mesophotic coral reef off southwestern Puerto Rico. This is only the fifth species of the family and the fourth of the genus Atergopedia Martinez Arbizu and Moura in Zool Beitr NF 38:189–210, 1998. The new species, Atergopedia dowgialli sp. nov., shares the following characters with its three congeners: 1) antenna without seta on the first endopodal segment; 2) lateral armature of the female antenna shows a multiplication of distinct setae and consists of one geniculate seta followed by five or more slender setae; 3) the first endopod segment of the first swimming leg P1 bears no inner seta; 4) male fifth leg with two spines on endopodal lobe; and 5) the exopod of the fifth swimming leg fused to basendopod in the female. Atergopedia dowgialli sp. nov. is characterized by the loss of the basal seta in both antenna and maxilliped, the loss of the first maxilla endite, the presence of a long process between the outer and distal elements of the leg 1 exopodite 3, which is stronger and longer in the female, and the possession of a very short and strongly ornamented furca in both sexes. The genus Atergopedia has been found in deep and relatively shallow habitats from the Arctic Ocean to the Caribbean. In contrast, almost all species of the taxon Novocrinidimorpha, which includes the families Novocriniidae, Superornatiremidae Huys 1996, and Rotundiclipeidae Huys 1988, have been found associated with anchialine caves, indicating a derived distribution for Atergopedia.

Temporally sustained dietary niche partitioning in two mesopelagic copepod species and their mouthpart morphology

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 518:51-67 (2015) - DOI: https://doi.org/10.3354/meps11045 Temporally sustained dietary niche partitioning in two mesopelagic copepod species and their mouthpart morphology Masayoshi Sano1,*, Yuichiro Nishibe1, Yuji Tanaka2, Shuhei Nishida1 1Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan 2Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan *Corresponding author: sano@aori.u-tokyo.ac.jp ABSTRACT: We compared seasonal feeding habit patterns, mouthpart structures, body sizes and vertical distributions of the aetideid copepods Undeuchaeta major and Chirundina streetsii in Sagami Bay, Japan. We focused on niche partitioning as one of the mechanisms sustaining co-existence of these mesopelagic copepods, by integrative application of stable-isotope analysis, microscopic observation and elemental analysis of the gut contents. While the body sizes and vertical distribution of both species were similar, they showed marked differences in their feeding habits throughout the year, i.e. carnivory in U. major and omnivory in C. streetsii, suggesting that food niche partitioning was a mechanism enabling their co-existence. Of particular interest was the consistently lower δ15N values in U. major, attributable to its selective feeding on oncaeid copepods. Previous reports of low δ15N in oncaeids, whose major food source has been assumed to be nanoplankton attached to appendicularian houses, suggest that the size of the primary producer is important in δ15N analyses of pelagic food webs. Comparison of mouthparts of the 2 species indicated slight differences in the maxilliped morphology. The relationship between feeding habit and maxilliped morphology was examined for the species above, other omnivorous aetideids and the Euchaetidae, a carnivorous sister family of the Aetideidae. The results suggest that carnivores have a broad basis and long unguiform setae, while omnivores have a narrower basis and shorter, more slender setae. The feeding habits of Aetideidae, which have so far been considered as exclusively omnivorous, comprise omnivory and carnivory, and can be distinguished by their maxilliped morphology. KEY WORDS: Aetideidae · Vertical distribution · Food chain · Marine snow · Feeding · Stable isotope · Elemental analysis Full text in pdf format Supplementary material PreviousNextCite this article as: Sano M, Nishibe Y, Tanaka Y, Nishida S (2015) Temporally sustained dietary niche partitioning in two mesopelagic copepod species and their mouthpart morphology. Mar Ecol Prog Ser 518:51-67. https://doi.org/10.3354/meps11045 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 518. Online publication date: January 07, 2015 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2015 Inter-Research.

Description of the immature stages of Sigara (Tropocorixa) santiagiensis (Hungerford, 1928) (Insecta: Heteroptera: Corixidae)

The immature stages of Sigara santiagiensis are described and illustrated for the first time. The eggs of Sigara can be distinguished by the chorionic surface. The main characters separating the five nymphal instars of S. santiagiensis are: body and head lengths; body, head and eye widths; ocular index; numbers of transverse sulcations of the rostrum, setae on the inner surface of protibiotarsus, campaniform sensilla on posterior surface of protrochanter, spines on posteroventral surface of mesotibia and on tibial comb of metaleg, long, slender setae on posterodorsal surface of mesotarsus, spines and swimming hairs on anteroventral and posterodorsal surfaces of metatarsus; and grade of development of the wing pads. The body length, the chaetotaxy of the mesonotum, profemora and metafemora, protibiotarsus, metatibia, metatarsus and urosternites distinguish species or group of species of instars I and V of some of the species of the genus Sigara described up to now.

A New Invasive Jack Beardsley Mealybug,Pseudococcus jackbeardsleyi(Hemiptera: Pseudococcidae) on Papaya in India

Mealybugs (Hemiptera: Pseudococcidae) trilocular pores on sclerotised area. All other cera are major pests of a wide range of agricultural, rii on a membranous area. Anterior cerari with horticultural and ornamental plants worldwide relatively smaller setae and with 2 to 3 auxiliary (Miller et al. 2002). Certain of attributes of the seate. Cerarii number C7 and cerarii on head with Pseudococcidae, viz., wide host range, short 3-4 conical setae. Dorsal surface with small se generation time, cosmopolitan nature, ability to ate and trilocular pores scattered almost evenly, transmit some important plant viruses, etc., have All frontal cerarri with oral rim tubular duct just contributed to their enormous damage potential behind them. Other oral rim tubular ducts pres (Meyer et al. 2008). In this regard, Pseudococcus ent submedially and submarginally on thorax jackbeardsleyi Gimpel and Miller (Hemiptera: and abdomen. Anal lobe and penultimate cerari Pseudococcidae), known as the Jack Beardsley with oral collar tubular duct between them. Ven mealybug, is a polyphagous species of neotropitral surface with slender setae. Multilocular disc cal origin that is known to attack 93 plant spepores present on V to VII abdominal segments, cies including several vegetable and fruit and Oral rim tubular duct and oral collar tubular ornamental crop species (CAB Intl. 2001). The ducts same as dorsum. (Fig. 1). earliest record of this mealybug in Asia was from The invasive mealybug is grayish in color; thin Singapore in 1958, followed by Malaysia in 1969, filaments around the body, caudal pair about one Indonesia in 1973, Philippines in 1975, Brunei in half of the length of the body, and ovisac cover 1979, Thailand in 1987, the Maldives, and Vieting hind part of the body. All other morphological nam in 1994 (Williams 2004a,b;Muniappan 2011) characters also match the description given by and not reported yet from India. In the current reWilliams (2004a). Quick and correct identifica port during the survey for monitoring the activity tion of an invasive pest like P. jackbeardsleyi is of papaya mealybug, Paracoccus marginatus Wilimportant in order to initiate suitable manage liams and Granara de Willink and its parasitoid ment measures that may include quarantine. Mo Acerophagus papayae Noyes & Schauff in Satyalecular identification, at this juncture is handy as mangalam (N 11.5200° E 77.2500°) Tamilnadu, it is not limited by life stage, polymorphism and India, a short tailed mealybug and P. marginatus gender. Incursion of this pest into India has been were found colonizing 2 papaya plantations on proven so far by identifying it by morphological or Jun 2012. Mealybugs were found scattered on molecular methods. Hence, in this paper we are the leaves, fruits and trunks and were observed providing evidence that identifies P. jackbeards sucking the sap from various parts of the papaya leyi in India by both morphological and molecular plants including leaves, stems, flowers and fruits, methods. No serious damage was observed on the papaya Standard protocols were followed for DNA ex plant caused by this new mealybug. Specimens traction, Polymerase Chain Reaction, sequencing of this mealybug were collected and preserved in of the mitochondrial cytochrome oxidase I (CO-I) ethyl alcohol (100%) and were stored at 20 °C ungene fragment and sequence alignment (Hajiba til further use. The mealybug was identified as baei et al. 2005). Small portion of the abdomen the Jack Beardsley mealybug, P. jackbeardsleyi, from individual P. jackbeardsleyi was used for at the National Bureau of Agriculturally Imporgenomic DNA and PCR was carried out in a ther tant Insects, Bangalore. mal cycler (ABI-Applied Biosystems, Veriti, USA) Morphological characters used for identifywith the following cycling parameters; 94 °C for 4 ing P. jackbeardsleyi are as follows: Body of the min as initial denaturation followed by 35 cycles female oval to elongate. Antenna 8 segmented, of 94 °C for 30 s, 47 °C for 45 s, 72 °C for 45 s and Legs well developed, translucent pores present on 72 °C for 20 min as final extension using univer hind femur and hind tibia. Discoidal pores pressal CO-I primers (LCO-14905'-GGT CAA CAA ent near eye on sclerotised rim. Cerarii 17 pairs. ATC ATA AAG ATA TTG G-3' and HCO-21985' Anal lobe cerari with 2 conical setae and many TAA ACT TCA GGG TGA CCA AAA AAT CA-3',