Slaughterhouse Research Articles

Lipid Peroxidation in Muscle Foods: Impact on Quality, Safety and Human Health.

The issue of lipid changes in muscle foods under the action of atmospheric oxygen has captured the attention of researchers for over a century. Lipid oxidative processes initiate during the slaughtering of animals and persist throughout subsequent technological processing and storage of the finished product. The oxidation of lipids in muscle foods is a phenomenon extensively deliberated in the scientific community, acknowledged as one of the pivotal factors affecting their quality, safety, and human health. This review delves into the nature of lipid oxidation in muscle foods, highlighting mechanisms of free radical initiation and the propagation of oxidative processes. Special attention is given to the natural antioxidant protective system and dietary factors influencing the stability of muscle lipids. The review traces mechanisms inhibiting oxidative processes, exploring how changes in lipid oxidative substrates, prooxidant activity, and the antioxidant protective system play a role. A critical review of the oxidative stability and safety of meat products is provided. The impact of oxidative processes on the quality of muscle foods, including flavour, aroma, taste, colour, and texture, is scrutinised. Additionally, the review monitors the effect of oxidised muscle foods on human health, particularly in relation to the autooxidation of cholesterol. Associations with coronary cardiovascular disease, brain stroke, and carcinogenesis linked to oxidative stress, and various infections are discussed. Further studies are also needed to formulate appropriate technological solutions to reduce the risk of chemical hazards caused by the initiation and development of lipid peroxidation processes in muscle foods.

Risk Factors for Leptospirosis in Rural Communities of Bungoma County, Kenya: A Cross Sectional Survey

Leptospirosis is an important re-emerging zoonosis of worldwide public health concern. Leptospirosis is caused by a bacteria of the genus Leptospira. There was an outbreak of leptospirosis in humans in Bungoma County in 2004 with severe consequences. Leptospirosis is enzootic in cattle in Bungoma County. This study was conducted to determine the risk factors for leptospirosis in humans in Bungoma County. The risk factors considered in this study were; rainfall patterns, livestock management practices (grazing and watering system), home slaughter of livestock, consumption of un-inspected meat, rodent infestation in homes, and frequency of contact of livestock keepers with veterinary extension staff. A cross-sectional survey was carried out in Kimilili and Mt Elgon sub-counties of Bungoma County, Kenya. The study was conducted between April and July of 2017. The locations were then sampled, and 400 households selected using a systematic random sampling technique. Questionnaires were administered to heads of the identified households. Descriptive statistics and the chi-square test were used to analyze the data. Secondary data from medical records in Kimilili and Webuye health facilities were taken to show the disease trends in the year 2004. The study reveals the risk factors for leptospirosis in Bungoma County which include the close association of the community and their livestock (87.9%, n=400), rodent infestation in homes (92.3%, n=400), slaughter of animals in non-designated areas (83.8%, n=400), consumption of un-inspected meat (83.0%, n=400) and low contact of the population with veterinary extension staff (63.7%, n=400). The risk factors under consideration in this study were observed in over 80% of the study population. The study recommends that knowledge of leptospirosis and its risk factors be enhanced for effective control and prevention program.

Comparison Study of Halal Management System in Indonesia and Malaysia

Halal standards between countries were varied, potentially leading to differences in halal product status decisions. In developing global halal standards, Indonesian and Malaysian halal standards were often used as references. This research aimed to gain a deeper understanding of the halal management systems in Indonesia and Malaysia in order to identify differences and similarities in halal regulations and management, including animal slaughtering practices. The halal management systems in Indonesia and Malaysia are mainly comparable. Some non-conforming differences include the certification procedure, certificate validity period, the halal products category, and the number of internal halal committees. Some essential differences are the halal certificate compulsoriness, stunning regulation, and the product name, brand, or synonym considered to be halal. The additional procedure used after slaughter, thoracic sticking, has become an emerging issue in developing a Mutual Recognition Agreement (MRA) of halal certificates for domestic products between Indonesia and Malaysia.

The Origins and Values of Javanese Philosophy in Nyadran with Goat-Cow Slaughter

This research aims to explore the origins of Nyadran, focusing on its ritualistic aspect involving the use of large animal parts (cows or goats), and to elucidate the underlying philosophy. Utilizing qualitative data from literature reviews and field studies, interviews were conducted to understand community perspectives. Findings reveal that Nyadran's implementation, involving slaughtering animals, lacks direct links to Islamic teachings and shows no explicit involvement of Islamic figures from the past. Instead, Nyadran's concepts stem from myths surrounding the village or the region in which it is organized. Community knowledge is grounded in beliefs about intertwined historical events and evolves with the growth of religious institutions. Justifications for Nyadran are based on philosophical arguments of religious, moral, and societal values, supporting the need for strong communal values and a harmonious life.

CONDITIONS OF CHANGES IN THE REGIONAL SPECIALIZATION OF ANIMAL PRODUCTION IN POLAND

Aim: The study aimed to identify regional specialization and changes in competitiveness in the field of animal production in Poland. Methods: The analysis covered statistical data of the Central Statistical Office from 2010–2021 on a national scale and in individual voivodeships. The study used a comparative analysis of animal production in regional terms. In order to determine the specialization of the region and to analyze changes in competitiveness over time, the classic method of Shift-Share Analysis (SSA) was used. Results: Major changes are taking place in Poland in the stock of farm animals and the production of animals for slaughter. In the years 2010–2021, the pig stock decreased drastically, and the stock of cattle, especially for fattening and poultry, increased. Changes in livestock stock contributed to an increase in beef cattle and poultry production and a decrease in the production of live pigs. Conclusions: There is specialization in Poland, and the concentration of production of the most important livestock species in individual voivodeships is increasing. Specialization and concentration processes allow for more effective use of farm resources and greater competitiveness of regions. However, the accelerating concentration of production may threaten the sustainable development of agriculture and negatively affect the natural environment. Therefore, further research on the impact of specialization and concentration of animal production on food security and the natural environment is recommended.

The Slaughter House and the Re-emerging Foodborne Illness with Special Reference to Bovine Tuberculosis

The Slaughter house is the place in which the animals are slaughtered for human consumption. The Slaughter house plays important role in prevention of zoonotic diseases between animals and humans like Mycobacterium tuberculosis as reemerging foodborne illness and also prevent infectious diseases between animals. Bovine Mycobacterium tuberculosis is caused by a species of pathogenic Gram positive, acid fast stain bacteria in the Mycobacteriaceae family. the causative agent bacteria of Bovine tuberculosis as reemerging foodborne illness tuberculosis bacteria has an waxy cover on its surface primarily due to the presence of acid called mycolic which refers the cells impervious to Gram staining, and as a result, the causative agent bacteria of Bovine tuberculosis as reemerging foodborne illness may appear weakly Gram-positive. Acid-fast bacilli by using certain stains called Ziehl Nielsen, or through using stain called fluorescent such as aura mine are used to identify the cause of Bovine tuberculosis as reemerging foodborne illness with a microscope. The Bacteria cause Bovine tuberculosis as a reemerging foodborne illness is aerobic bacteria and needs high concentrations of oxygen. Mainly this bacteria is pathogenic to human and mammal's respiratory system, it infects the lungs. The most diagnostic means for Bovine tuberculosis as a reemerging foodborne illness are the tuberculin skin examination, stain of acid-fast, laboratory culture, and through using polymerase chain reaction method.

The Emission Characteristics of Greenhouse Gases from Animal Husbandry in Shandong Province Based on Life Cycle Assessment

Life cycle assessment was used to study the following six major stages of animal husbandry: feed grain planting, feed grain transportation and processing, livestock and poultry breeding, livestock and poultry gastrointestinal fermentation, manure management, and livestock and poultry product slaughter and processing. The greenhouse gas emissions from animal husbandry in Shandong Province were quantified for the entire 20-year period spanning from 2002 to 2021. This study also analyzed the emission patterns and characteristics associated with this life cycle assessment. The results show that over the past 20 years, the greenhouse gas emissions from animal husbandry in Shandong Province increased continuously, the greenhouse gas emission intensity decreased continuously, and both of these trends tended to be stable. From a life cycle standpoint, the primary sources of greenhouse gas emissions were gastrointestinal fermentation and the management of livestock and poultry manure. In terms of the structure of livestock and poultry breeding, poultry was the primary source of greenhouse gas emissions. The emission characteristics of the greenhouse gases produced by animal husbandry varied among different cities in Shandong Province. The main source of greenhouse gas discharged due to animal husbandry in Zibo and Binzhou was Ecattle; in Dongying, it was Esheep; and in the remaining cities, it was mainly Epoultry.

Concurrent Infection of Oesophagostomum species in a Slaughtered Goat: A Case Report

The morbidity and mortality of gastrointestinal nematodes in goats made them one of the most significant parasites in the world, causing huge economic loss for farmers. It has been reported that the economic loss due to gastrointestinal parasitism was Rs. 372.35 per goat annually. In the present study, the intestinal sample was collected from a local slaughter house at Izatnagar, Bareilly, Uttar Pradesh, for screening the gastrointestinal nematodes. Initially, the pathological lesions like nodules, cyst and haemorrhagic patches etc. were examined for any parasitic infection. The found worms in the intestinal content were subjected for microscopic examination for the identification of the nematode and identified them as Oesophagostomum spp. based on buccal capsule with cephalic and cervical structures. Interestingly, both Oesophagostomum columbianum and O. asperum species were found in the same sample. The male and female Oesophagostomum species were also differentiated by examining the spicule and gubernacular length, position of the vulva and anus. This study reports the infection of Oesophagostomum spp. in a goat and the first case report of concurrent infection of O. columbianum and O. asperum in the Bareilly region of Uttar Pradesh.

Non-aureus staphylococci and mammaliicocci (NASM): their role in bovine mastitis and One Health.

Non-aureus staphylococci (NAS) are gaining importance in mastitis and public health, and some NAS have been reclassified as mammaliicocci (NASM). Bovine milk production has a major influence on the world economy, being an essential source of income for small, medium and large producers, and bovine mastitis caused by NASM can cause an economic impact. Mastitis generates financial losses due to reduced revenue, increased veterinary costs and expenses associated with animal slaughter. However, it is also a public health issue involving animal health and welfare, human health and the ecosystem. Furthermore, it is an increasingly common infection caused by NASM, including antimicrobial-resistant strains. Despite all these adverse effects that NASM can cause, some studies also point to its protective role against mastitis. Therefore, this review article addresses the negative and positive aspects that NASM can cause in bovine mastitis, the virulence of the disease and resistance factors that make it difficult to treat and, through the One Health approach, presents a holistic view of how mastitis caused by NASM can affect both animal and human health at one and the same time.

The Reasons Behind Long-Term Endemicity of Brucellosis in Low and Middle-Income Countries: Challenges and Future Perspectives.

Brucellosis is a neglected worldwide zoonotic disease with more than 500,000 new human cases each year. Direct contact with infected animals and consumption of undercooked animal origin foods are the main routes of brucellosis transmission to humans. Although long endeavor has been applied to control and eliminate brucellosis from animal and human populations in developing countries especially in low- and middle-income countries (LMICs), the disease is still endemic in these regions. Many common or unique factors including raw milk consumption, unhygienic slaughter of livestock, extensive husbandry, budgetary limitations, misdiagnosis, and other conditions play a role in long-term endemicity of brucellosis in these locations. It has been shown that One Health is the only practical approach to control brucellosis; however, applying such methods is challenging in low-resource areas. In such conditions, brucellosis is continuously maintained in animals and repeatedly spread to human populations. In this article, factors playing a critical role in brucellosis endemicity, and the real conditions challenging the application of One Health approach in control of brucellosis are highlighted.

Validity of pig lung culling by veterinary rules of animal slaughter NO. 68718 DATED 06/02/2022

The purpose of the study is to determine the validity of culling of pigs with morphological changes that occur when stunned before slaughter by carbon dioxide, according to Veterinary Rules of animal slaughter No. 68718 02.06.2022. As the research material, lung samples of 8 healthy slaughter pigs aged 6.5-7.5 months, weighing 95-105 kg, including 3 pigs stunned by electric shock, and from 5 pigs that were stunned by carbon dioxide were used. The samples were fixed in a 10% solution of neutral formalin. Histological sections were made, they were stained with hematoxylin and eosin. In the lungs of 3 pigs, which were stunned with electric shock and then exsanguinated, during post-slaughter examination, a uniform staining of the organ in light red color, elastic consistency was established, which corresponded to a normal lung. In the lungs of 5 pigs, which were stunned with carbon dioxide and then exsanguinated, heterogeneous staining was established during post-slaughter examination; there were areas of dark red color and compacted consistency, frothy liquid was released from the surface of the incision. In histological preparations of the lungs of pigs that were stunned with carbon dioxide, edema of the alveoli and congestive hyperemia of blood vessels were detected, which should be recognized as the result of insufficient exsanguination. In histological preparations of the lungs of pigs, which were stunned with electric shock, fullness of blood vessels and pulmonary alveolar edema were found. The rules of veterinary and sanitary examination do not indicate the culling of lungs with hyperemia and edema, that is, they are not subject to disposal and destruction. Assuming the exact execution of directive documents and careful treatment of animal slaughter products, it makes sense to officially recognize such lungs as either subject to destruction or suitable for a certain use.

Seroprevalence of Leptospirosis in Cattle in Smallholder Livestock Production Systems in Bungoma County, Kenya

Leptospirosis is an important re-emerging bacterial zoonosis of public health importance in Kenya. It is transmitted through contact with contaminated soil, water, or urine of infected animals. The disease is associated with high economic losses which include high cost of diagnosis and treatment, disruption of international trade in animals and animal products and loss of milk production following mastitis among others. In this study, the sero-prevalence of bovine leptospirosis in Kimilili and Mt Elgon Sub-Counties of Bungoma County in Kenya was estimated. A cross-sectional study was conducted between April to July, 2017 where blood serum samples from 200 head of cattle from five wards and one slaughter house using systematic random sampling technique. The samples were then subjected to the Enzyme Linked Immunorsorbent Assay (ELISA) test. The findings show an overall sero-prevalence for leptospirosis in cattle in the study area to be 16%. The sero-prevalence of leptospirosis in the study area by wards is as follows; 10.7% in Kibingei, 12.5% in Kimilili, 17.6 in Maeni, 16.7% in Kamukuywa and 15.6% in Kaptama. The study also reveals that the sero-prevalence was relatively higher in Kimilili Slaughterhouse which recorded 21.0%. The study therefore demonstrates that there is a high sero-prevalence of leptospirosis in cattle in Kimilili and Mt Elgon Sub-Counties, Bungoma County. This study therefore provides critical public health information which is necessary in guiding leptospirosis prevention and control in Bungoma County. It is therefore recommended that strategies for surveillance and laboratory diagnosis of leptospirosis should be initiated in Bungoma County. This will help in providing better estimates of leptospirosis burden in the county.

Non-animal protein hydrolysates from agro-industrial wastes: A prospect of alternative inputs for cultured meat

In light of the growing demand for alternative protein sources, laboratory-grown meat has been proposed as a potential solution to the challenges posed by conventional meat production. Cultured meat does not require animal slaughter and uses sustainable production methods, contributing to animal welfare, human health, and environmental sustainability. However, some challenges still need to be addressed in cultured meat production, such as the use of fetal bovine serum for medium supplementation. This ingredient has limited availability, increases production costs, and raises ethical concerns. This review explores the potential of non-animal protein hydrolysates derived from agro-industrial wastes as substitutes for critical components of fetal bovine serum in cultured meat production. Despite the lack of standardization of hydrolysate composition, the potential benefits of this alternative protein source may outweigh its disadvantages. Future research holds promise for increasing the accessibility of cultured meat.

Driving forces shaping the microbial ecology in meat packing plants.

Meat production is a complex system, continually receiving animals, water, air, and workers, all of which serve as carriers of bacteria. Selective pressures involved in different meat processing stages such as antimicrobial interventions and low temperatures, may promote the accumulation of certain residential microbiota in meat cutting facilities. Bacteria including human pathogens from all these sources can contaminate meat surfaces. While significant advancements have been made in enhancing hygienic standards and pathogen control measures in meat plants, resulting in a notable reduction in STEC recalls and clinical cases, STEC still stands as a predominant contributor to foodborne illnesses associated with beef and occasionally with pork. The second-and third-generation sequencing technology has become popular in microbiota related studies and provided a better image of the microbial community in the meat processing environments. In this article, we reviewed the potential factors influencing the microbial ecology in commercial meat processing facilities and conducted a meta-analysis on the microbiota data published in the last 10 years. In addition, the mechanisms by which bacteria persist in meat production environments have been discussed with a focus on the significant human pathogen E. coli O157:H7 and generic E. coli, an indicator often used for the hygienic condition in food production.

The impact of COVID‐19 on price transmission and price volatility in the Canadian beef supply chain

AbstractThe COVID‐19 pandemic resulted in disruptions to the Canadian meat and livestock markets. While prices initially decreased, the shutdowns of beef packing plants led to a large reduction in the supply of beef and a corresponding increase in the wholesale price with ramifications along the entire supply chain. This study examines the effect of COVID‐19 on price transmission and price volatility in the Canadian beef supply chain using monthly farmgate, wholesale, and retail price data covering the period from July 2005 to February 2022. We find evidence indicating that COVID‐19 affected long‐run price transmission from farmgate and wholesale markets to the retail market. Specifically, we find that the pandemic resulted in a 94.42 and 81.48% decrease in price transmission from the farmgate and wholesale market, respectively, to the retail market, indicating that higher prices at the wholesale level were not being passed on to consumers to the same extent. In the short run, we find asymmetric price adjustments and both direct and indirect volatility spillovers among the three levels of markets, implying strong market interactions across the beef supply chain. Overall, our results suggest the resiliency of the Canadian beef sector to COVID‐19 shocks.

Ways of Food Contamination, Its Impact and Prevention

Cross food contamination means transportation of bacteria or other microorganisms from contaminated substance to the food. Cross food contamination can happen during any stage of food production. Each year, a large number of people’s worldwide experience a foodborne illness, while there are number of causes, a most and common preventable one is cross food contamination. Cross food contamination as Bacterial cross food contamination is defined as the transportation of bacteria or other microorganisms from contaminated substance to the food. Other types of cross food contamination include the transportation of food allergens, chemicals, or toxins. Foodborne illness may cause by eating at restaurants, but there are many ways in which cross food contamination can occur, including, primary food production, from plants and animals on farms, during harvest or slaughtering of food animals, secondary food production including food processing and manufacturing, transportation of food, storage of food, distribution of food, grocery stores, farmer’s markets, and more, food preparation and serving at home, restaurants, and other foodservice operations, Given that there are many points at which cross food contamination can occur, it’s important to learn about the different types and how we can prevent cross food contamination.

Prevalence and risk factors of Linguatula spp. in slaughter animals in Tabriz, Iran, and methods for nymphal stage inactivation

Linguatula is a food born zoonotic parasite in which carnivores and herbivores serve as final and intermediate hosts, respectively. Human infection with Linguatula spp. occurs following consumption of raw or undercooked infected internal organs of the intermediate host and/or consumption of water and/or vegetables contaminated with eggs released from final hosts. The aim of this study was to determine prevalence and risk factors of Linguatula spp. in sheep, cattle, buffalo, goats and camels slaughtered at the Tabriz abattoir, Iran. In addition, effect of temperature and sodium chloride (NaCl) on survival time of Linguatula spp. nymphs was assessed. For this purpose, 25,520 mesenteric lymph nodes from 2552 animals and the livers and lungs from 656 animals were collected randomly and examined. To evaluate the effect of temperature and NaCl on the survival of Linguatula spp. nymphs in infected livers and lungs, 30 g of each liver and lung with dimensions of 2 × 3 × 4 cm, were exposed to temperatures of −20, 10, 50, 60 and 72 °C and NaCl concentrations of 5 %, 10 %, 15 % and 20 % for 3, 6, 12, 24, 48 and 72 h, in triplicate. Based on the mesenteric lymph nodes, 25.7 % (656 of 2552 animals) were infected with Linguatula spp. Of the 656 liver and lungs assessed, 141 (21.5 %) and 62 (9.5 %) were infected with Linguatula spp., respectively. The rate of infection of mesenteric lymph nodes in all animals was significant with age (P < 0.05), with more older animals infected. In regards to sex, except for camels, more female animals were infected than male animals (P < 0.05). There was a significant difference in survival of nymphs based on temperature and/or NaCl and time (P < 0.0001). At 72 h, all temperatures assessed except 10 °C, resulted in all nymphs being inactivated. Sodium chloride was more effective against Linguatula spp. nymphs in livers than in lungs with 100 % efficacy only achieved against nymphs in livers at 20 % concentration after 48 h and at 10 and 15 % concentration after 72 h. Based on these results, heating and application of common salt as a food preservative in meat products reduces the survival time of Linguatula spp. nymphs and their use could decrease the risk of food-born microorganisms.

Изучение периода выведения флорфеникола и его метаболитов из организма поросят после применения препарата «Флорипрем 40»

Purpose. Determination of the period of elimination of residual amounts of florfenicol and its metabolites in the form of florfenicolamine from the organs and tissues of pigs after the use of the veterinary drug "Floriprem 40". Materials and Methods. The study of the period of elimination of residual amounts of the active substance of the drug was carried out on the basis of a pig-breeding complex in the Kaluga region. For research work, 21 animals (20 experimental, 1 control) were used at the age of 86 days with a body weight of 44-49 kg. The drug was given to piglets during 7 days in a group method in a mixture with compound feed at a dose of 5 kg of the drug per 1 ton of feed. The slaughter of animals (5 animals per term) was carried out 8, 12, 14, 16 days after the last administration of the drug. Samples of muscle tissue (right thigh), liver, kidneys and skin with subcutaneous and fatty cellulose were collected from each animal in individual bags. The content of florfenicol and its metabolites in the form of florfenicolamine in extracts of samples of organs and tissues of piglets was determined by high-performance liquid chromatography; residual amounts of florfenicol in organs and tissues of animals were determined using acid hydrolysis. The study was carried out using a Shimadzu LCMS-8050 chromatography-mass spectrometry system (Japan). Data analysis and processing were carried out using the Shimadzu LabSolutions software package (Realtime Analysis, Postrun Analysis, Quant Browser) v.5.99 SP2 (Japan). Results. The results of a study of the dynamics of excretion of residual amounts of florfenicol and its metabolites from organs and tissues of pigs showed that florfenicolamine accumulates mainly in the liver and kidneys; insignificant levels of florfenicolamine were found in the muscles of pigs. The data obtained indicate a high degree of adsorption of the active substance of the test drug into the organs and tissues of pigs and a long-term presence in the body. Florfenicol and its metabolites were not detected in skin tissues with subcutaneous fat of pigs. An assessment of the elimination time of florfenicol and its metabolites from the body of pigs, carried out using the WT1.4 program, showed that the elimination period of the active substance is: in the muscles – 5 days, in the liver – 14 days, in the kidneys – 10 days. Conclusion. Consumption of animal products obtained after a course of oral administration of the drug "Floriprem 40" in pigs does not pose a danger to human health 14 days after the end of the drug.

Development of triplex assay for simultaneous detection of Escherichia coli, methicillin resistant and sensitive Staphylococcus aureus in raw pork samples of retail markets.

Escherichia coli and Staphylococcus aureus are the most important food borne pathogen transmitting from animal meat and meat products. Therefore, it is vital to design an accurate and specific diagnostic tool for identifying those food-borne pathogens in animal meat and meat products. In the current study, E. coli, methicillin-resistant and sensitive S. aureus (MRSA and MSSA) were simultaneously detected using a developed triplex PCR-based technique. To obtain an optimal reaction parameter, the multiplex assay was optimised by changing just one parameter while holding the others constant. Specificity of the assay was assessed using several porcine bacterial template DNA. The plasmid DNA was used to test the multiplex PCR assay's sensitivity and interference in spiked pork samples. E. coli, MRSA, and MSSA each have PCR amplified products with sizes of 335, 533, and 209bp, respectively. The assay detects a minimum microbial load of 102CFU/μl for all the three pathogens and can identify bacterial DNA as low as 10-2ng/µl. The assay was validated employing 210 pork samples obtained from retail meat shops and slaughter houses, with MRSA, E. coli, and MSSA with the occurrence rate of 1.9%, 42.38%, and 18.1%, respectively. The rate of mixed bacterial contamination in pork meat samples examined with the developed method was 6.19%, 1.43%, 1.90%, and 1.43% for MSSA & E. coli, MRSA & E. coli, MSSA & MRSA, and E. coli, MSSA & MRSA, respectively. The developed multiplex PCR assay is quick and efficient, and it can distinguish between different bacterial pathogens in a single reaction tube.

Prevalence and characterization of non-typhoidal Salmonella in egg from grading and packing plants in Korea

Egg washing guidelines vary across countries; however, since 2020, Korea has required that all eggs produced from farms with more than 10,000 laying hens must be washed through egg grading and packing (GP) plant. This study investigated the prevalence and characterization of non-typhoidal Salmonella in eggs after washing at GP plants. In total, 16,800 eggs were collected from 60 egg GP plants located inside commercial layer farms, and 840 pooled eggshell and egg contents were tested for Salmonella, respectively. Of the 60 GP plants tested, 11 (18.3%) and 12 (20.0%) plants were positive for Salmonella spp. In the eggshells and egg contents, respectively. In particular, High Salmonella prevalence in the eggshells and egg contents occurred most often in farms with laying hens older than 80 weeks (33.3% and 40.0%, respectively). However, among 840 pooled eggshells and egg content samples, only 19 (2.3%) of each sample type were positive only for non-typhoidal Salmonella spp. The most common Salmonella serovar in both eggshells and egg contents was S. Infantis, which was found in five (8.3%) of 60 GP plants for both samples types. The other Salmonella serovars detected in eggshells were S. Bareilly (5.0%), S. Agona (3.3%), S. Enteritidis (1.7%), and S. Montevideo (1.7%), whereas those detected in egg contents were S. Enteritidis (5.0%), S. Agona (3.3%), S. Newport (3.3%), S. Senftenberg (3.3%), and S. Derby (1.7%). Of the 19 virulence genes tested, 14 genes were detected in all Salmonella. Interestingly, the spvB gene was detected only in S. Enteritidis, and the sefC gene was detected only in S. Enteritidis and S. Senftenberg. Moreover, all S. Infantis isolates showed multidrug resistance (MDR) against five or more classes, and the other serovars only showed MDR against three to four classes or no MDR. These results suggest that comprehensive surveillance and advanced management approaches for egg GP plants are required to minimize egg contamination with non-typhoidal Salmonella.