Skin Imprints Research Articles

Comparing plasma and skin imprint metabolic profiles in COVID-19 diagnosis and severity assessment.

As SARS-CoV-2 continues to produce new variants, the demand for diagnostics and a better understanding of COVID-19 remain key topics in healthcare. Skin manifestations have been widely reported in cases of COVID-19, but the mechanisms and markers of these symptoms are poorly described. In this cross-sectional study, 101 patients (64 COVID-19 positive patients and 37 controls) were enrolled between April and June 2020, during the first wave of COVID-19, in São Paulo, Brazil. Enrolled patients had skin imprints sampled non-invasively using silica plates; plasma samples were also collected. Samples were used for untargeted lipidomics/metabolomics through high-resolution mass spectrometry. We identified 558 molecular ions, with lipids comprising most of them. We found 245 plasma ions that were significant for COVID-19 diagnosis, compared to 61 from the skin imprints. Plasma samples outperformed skin imprints in distinguishing patients with COVID-19 from controls, with F1-scores of 91.9% and 84.3%, respectively. Skin imprints were excellent for assessing disease severity, exhibiting an F1-score of 93.5% when discriminating between patient hospitalization and home care statuses. Specifically, oleamide and linoleamide were the most discriminative biomarkers for identifying hospitalized patients through skin imprinting, and palmitic amides and N-acylethanolamine 18:0 were also identified as significant biomarkers. These observations underscore the importance of primary fatty acid amides and N-acylethanolamines in immunomodulatory processes and metabolic disorders. These findings confirm the potential utility of skin imprinting as a valuable non-invasive sampling method for COVID-19 screening; a method that may also be applied in the evaluation of other medical conditions. KEY MESSAGES: Skin imprints complement plasma in disease metabolomics. The annotated markers have a role in immunomodulation and metabolic diseases. Skin imprints outperformed plasma samples at assessing disease severity. Skin imprints have potential as non-invasive sampling strategy for COVID-19.

Evaluation of the potential role of skin contact and transfer in the Maillard hypothesis of image formation on the Shroud of Turin

The mechanism for creation of the faint ventral and dorsal images of a man on the Shroud of Turin is unknown. It has been suggested that image formation involves oxidation and dehydration of the cellulose present in the linen, or, alternatively, that a superficial residue on the surface of the cloth is responsible for creating colored products through the Maillard reaction. One of the major criticisms of the latter hypothesis is that gaseous diffusion is not sufficient to result in the detailed bodily structures present on the cloth. Recently, it was proposed that additional substrates might also participate in the Maillard reaction, specifically proteins and amino acids present in skin, helping to increase the detail of any image produced. Understanding more fully the properties of the Shroud image is important for the preservation of this cloth for future generations. In the current report the potential contribution of skin proteins and amino acids in the Maillard hypothesis of image formation was evaluated. Using a variety of systems, these data show that detailed imaging of skin imprints may be obtained through contact transfer and interaction with reducing sugars. These results importantly extend the scope of this hypothesis and provide additional, relevant information concerning possible mechanisms for the formation of the Shroud of Turin image.

Captorhinid trackways from mid- to late Permian red beds in Morocco: Implications for locomotion and the palaeobiogeography of northwest Gondwana

In this paper, we report a new vertebrate ichnoassemblage from the mid- to late Permian red beds of the Ikakern Formation (Tourbihine Member, T2) from the Argana Basin of Morocco, to improve knowledge of paleobiology and palaeobiogeography. The ichnofossils comprise than 50 footprints organized as four trackways and assigned to the ichnogenus Hyloidichnus. The tetrapod tracks are preserved in convex hyporelief and concave epirelief on upper and lower mud-rich surfaces near the top of fine-grained sandstone units. The trackway pattern and the alternating arrangement of the pes-manus sets suggest a sprawling and symmetrical gait, with the movements of the front and hind limbs being of similar size, and it is likely that the animal moved at slow trot. We infer that the potential trackmaker was a captorhinomorph within the Moradisaurinae. This new fossil discovery helps reconstruct the palaeoenvironments and the palaeogeographic distribution of these Permian organisms, with Morocco acting as a faunal exchange gateway between western Gondwana and western Laurasia. Besides the tetrapod tracks, the red beds contain ubiquitous rain-drop marks, current ripples, plant fossil impressions, invertebrate burrows, tetrapod skin imprints, and a single insect wing that suggest that the strata were deposited in an episodically inundated alluvial plain.

3D-printed external cranial protection following decompressive craniectomy after brain injury: A pilot feasibility cohort study.

3D-printed (3DP) customized temporary cranial protection solutions following decompressive craniectomy (DC) are currently not widely practiced. A pilot trial of a 3DP customized head protection prototype device (HPPD) on 10 subjects was conducted during the subacute rehabilitation phase. Subjects > 30 days post-DC with stable cranial flaps and healed wounds were enrolled. HPPD were uniquely designed based on individuals' CT scan, where the base conformed to the surface of the individual's skin covering the cranial defect, and the lateral surface three-dimensionally mirrored, the contralateral healthy head. Each HPPD was fabricated using the fused deposition modeling method. These HPPD were then fitted on subjects using a progressive wearing schedule and monitored over 1, 2, 4, 6 and 8 follow-up (FU) weeks. Outcomes during FU included; reported wearing time/day (hours), subjective pain, discomfort, pruritus, dislodgment, cosmesis ratings; and observed wound changes. The primary outcome was safety and tolerability without pain or wound changes within 30 minutes of HPPD fitting. In all, 10 enrolled subjects received 12 HPPDs [5/10 male, mean (SD) age 46 (14) years, mean (SD) duration post-DC 110 days (76)] and all subjects tolerated 30 minutes of initial HPPD fitting without wound changes. The mean (SD) HPPD mass was 61.2 g (SD 19.88). During 8 weeks of FU, no HPPD-related skin dehiscence was observed, while 20% (2/10) had transient skin imprints, and 80% (8/10) reported self-limiting pressure and pruritis. Findings from this exploratory study demonstrated preliminary feasibility and safety for a customized 3DP HPPD for temporary post-DC head protection over 8 weeks of follow-up. Monitoring and regular rest breaks during HPPD wear were important to prevent skin complications. This study suggests the potential for wider 3DP technology applications to provide cranial protection for this vulnerable population.

Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

INTRODUCTION: We diagnose cases of cutaneous leishmaniasis (CL) among indigenous peoples of the state of Roraima, Brazil, and discuss some aspects of its epidemiology. METHODS: Skin imprints, and lesion exudate samples collected on filter paper were examined using parasitological and molecular techniques, respectively. RESULTS: Of 30 indigenous individuals, representing several ethnic groups, with suspected cases of CL, 27 (90%) tested positive for Leishmania spp. by PCR, and 21 (70%) by parasitological microscopy.CONCLUSIONS: Cutaneous leishmaniasis is indistinctly present among indigenous peoples from different regions of the state of Roraima. Individuals from seven of the ten existing ethnic groups in the state tested positive for CL, demonstrating the need for further investigation of the disease among these ethnic groups.

Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening.

Cystic fibrosis (CF) is a disabling genetic disease with an increased prevalence in European heritage populations. Currently, the most used technique for collection of CF samples and diagnosis is provided through uncomfortable tests, with uncertain results, mostly based on chloride concentration in sweat. Since CF mutation induces many metabolic changes in patients, exploring these alterations might be an alternative to visualize potential biomarkers that could be used as interesting tools for further diagnostic upgrade, prioritizing simplicity, low cost, and quickness. This contribution describes an accurate strategy to provide potential biomarkers related to CF, which may be understood as a potential tool for new diagnostic approaches and/or for monitoring disease evolution. Therefore, the present proposal consists of using skin imprints on silica plates as a way of sample collection, followed by direct-infusion high-resolution mass spectrometry and multivariate data analysis, intending to identify metabolic changes in skin composition of CF patients. Metabolomics analysis allowed identifying chemical markers that can be traced back to CF in patients' skin imprints, differently from control subjects. Seven chemical markers from several molecular classes were elected, represented by bile acids, a glutaric acid derivative, thyrotropin-releasing hormone, an inflammatory mediator, a phosphatidic acid, and diacylglycerol isomers, all reflecting metabolic disturbances that occur due to of CF. The comfortable method of sample collection combined with the identified set of biomarkers represent potential tools that open the range of possibilities to manage CF and follow the disease evolution. This exploratory approach points to new perspectives about the development of diagnostic assay using biomarkers and the management CF.

Influences of age, ethnic group, and skin sites on a provisory skin marking, experimentally induced, in vivo.

To study capacity of skin to being imprinted by a marking head rapidly applied (10seconds) onto the skin under a standardized pressure (15kPa). Referential photographs of skin markings, resulting from different times of application serve at grading the extent of skin imprints. The imprinting tool, equipped with 5 linear bulbs of different sizes but same thickness (1mm), was applied onto the cheeks of 641 women of different ages (18-80years) of 3 different ethnic groups. In the cohort of 198 Caucasian women, the marking head was pressed onto 3 different sites (outer arm, cheek, and dorsal forearm) and the kinetics of skin recovery from cheek marking was recorded on a smaller and younger part of this Caucasian cohort, ie, 141 Caucasian women, aged 18-59years. The recorded intensities of skin markings were found highly correlated with age in all 3 studied ethnic groups (Chinese, African-American, and Caucasian). Caucasian women seem more sensitive to the marking and the kinetics of recovery from such imprint was found highly dependent from its intensity, ie, the higher the grades, the slower the recovery. In Caucasian women, intensities were different between skin sites, where arm showed higher resistance to marking. Possible impact of photo-aging on the decreased skin resistance to such provoked deformation is likely of minor amplitude, as compared to the one induced by chronological aging. This method offers a new vision on skin aging, in its variable response to a standard deformation that appears mostly driven by chronological aging.

Skin imprinting in silica plates: a potential diagnostic methodology for leprosy using high-resolution mass spectrometry.

Leprosy is a chronic infectious disease caused by Mycobacterium leprae, which primarily infects macrophages and Schwann cells, affecting skin and peripheral nerves. Clinically, the most common form of identification is through the observation of anesthetic lesions on skin; however, up to 30% of infected patients may not present this clinical manifestation. Currently, the gold standard diagnostic test for leprosy is based on skin lesion biopsy, which is invasive and presents low sensibility for suspect cases. Therefore, the development of a fast, sensible and noninvasive method that identifies infected patients would be helpful for assertive diagnosis. The aim of this work was to identify lipid markers in leprosy patients directly from skin imprints, using a mass spectrometric analytical strategy. For skin imprint samples, a 1 cm(2) silica plate was gently pressed against the skin of patients or healthy volunteers. Imprinted silica lipids were extracted and submitted to direct-infusion electrospray ionization high-resolution mass spectrometry (ESI-HRMS). All samples were differentiated using a lipidomics-based data workup employing multivariate data analysis, which helped electing different lipid markers, for example, mycobacterial mycolic acids, inflammatory and apoptotic molecules were identified as leprosy patients' markers. Otherwise, phospholipids and gangliosides were pointed as healthy volunteers' skin lipid markers, according to normal skin composition. Results indicate that silica plate skin imprinting associated with ESI-HRMS is a promising fast and sensible leprosy diagnostic method. With a prompt leprosy diagnosis, an early and effective treatment could be feasible and thus the chain of leprosy transmission could be abbreviated.

Dinosaur tracks from the Jurassic Shemshak Group in the Central Alborz Mountains (Northern Iran)

Abstract The Shemshak Group includes alternating layers of coal-bearing shale and siliciclastic sediments in the Baladeh area in the central Alborz Mountains of northern Iran. A diverse and abundant Jurassic dinosaur footprint assemblage is now recognized in the group, which is Toarcian to Bajocian in age in the northern Baladeh. This is the first report of a diverse dinosaur ichnoassemblage from Iran that includes the footprints of sauropods. These tracks can be assigned to three groups of trackmakers: theropods, ornithopods and sauropods. Those of theropods are typically tridactyl in shape, their trackways reflecting bipedal movement. Theropod footprints are very abundant in both northern and western Baladeh. The studied theropod tracks themselves are divided into three major dimensional groups. The medium sized footprints (footprint length, 11-15 cm) are abundant and have a stride length, digit and pace angles like the coelurosaurs footprints and trackway. Theropod footprints were identified as similar to Schizograllator otariensis, Talmontopus tersi and Wildeichnus isp. Ornithopod footprints are tridactyl with rounded and thick toes and belong to bipeds. Some didactyl imprints were also observed. Skin imprints were well preserved in these footprints. The ornithopod tracks resemble Jiayinosorupus johnsoni, as well as Velociraptorichnus sichuanensis for didactyl footprints. Sauropod footprints found in the western part of Baladeh are assigned here to Eosauropus isp., which are pentadactyl pes imprints of a quadruped. The assemblage from Iran resembles similar associations from eastern Asia.

Kyrgyzsaurus bukhanchenkoi gen. et sp. nov., a new reptile from the triassic of southwestern Kyrgyzstan

A new reptile, Kyrgyzsaurus bukhanchenkoi gen. et sp. nov., from the Triassic (Madygen Formation) of southwestern Kyrgyzstan is described based on the anterior part of the skeleton (skull, cervical and anterior dorsal vertebrae, ribs, pectoral girdle) and skin imprints. This is the most archaic representative of the family Drepanosauridae (Archosauromorpha, Diapsida). The most prominent features of the new form are the shortened lower jaw, numerous teeth, granular body osteoderms, large supraorbital shelflike skin folds, and thick and extensive throat sac.

Sézary Syndrome in a Cat

Sézary syndrome is an uncommon leukemic variant of cutaneous lymphoma in cats. This cat had recurrent dermatitis with erythematous, pruritic plaques. Multiple skin imprints and biopsy samples were obtained over a 6-month period, and histopathological findings were consistent initially with eosinophilic miliary dermatitis and later with erythema multiforme. One week before death, Sézary cells were identified in the peripheral blood that expressed cluster of differentiation (CD)3 and CD8 antigens. Massive infiltration of CD3+ lymphocytes was noted in the skin and multiple internal tissues by histopathological examination. This case demonstrates the difficulty in diagnosing cutaneous lymphoma early in the disease course.

Palaeontology: pterosaur embryo from the Early Cretaceous.

Dinosaur embryos have been discovered all over the world, but so far no pterosaur embryos have been reported. Here we describe a Chinese fossil from the Early Cretaceous period containing an embryo that is unambiguously a pterosaur. The embryonic skeleton, which is exquisitely preserved in its egg, is associated with eggshell fragments, wing membranes and skin imprints. This discovery confirms that pterosaurs were egg-layers and sheds new light on our understanding of pterosaur development.

Experimental transmission of cutaneous chytridiomycosis in dendrobatid frogs.

In a series of three experiments during March-October, 1998, two species of captive-bred poison dart frogs (Dendrobates tinctorius and D. auratus) were exposed to Batrachochytrium dendrobatidis, a recently-described chytridiomycete fungus (chytrid) that was originally isolated from a blue poison dart frog (D. azureus). All frogs exposed to the chytrids developed a fatal skin disease, whereas none of the control frogs developed skin lesions. The most consistent clinical sign in chytrid-exposed frogs was excessive shedding of skin. Gross lesions were subtle, usually affected the legs and ventrum, and consisted of mild skin thickening and discoloration. Microscopic examination of shed skin pieces and/or skin imprints demonstrated the presence of chytrids and was used for ante mortem and post mortem confirmation of chytrid infection. Histologically, there was epidermal hyperkeratosis, hyperplasia, and hypertrophy associated with low to moderate numbers of chytrids in the keratinized layers. These experiments demonstrated that Batrachochytrium dendrobatidis can be a fatal pathogen in poison dart frogs. The experimentally-induced disease in these frogs resembled cases of cutaneous chytridiomycosis that have recently been described in several other species of captive and wild amphibians.

Synaptichnium tracks with skin impressions from the Anisian (middle Triassic) of the Southern alps (val di non ‐ Italy)

In Val di Non (northern Italy), siltstones of Anisian age (middle Triassic) yield archosaurian and other reptilian tracks which show excellent details of their scaly plantar and palmar surfaces. The ichnofauna includes Rhynchosauroides, Chirotherium, Brachichiroth‐erium, Isochirotherium and Synaptichnium as well as tracks which can be ascribed to procolophonid reptiles and arthropod trails. The excellent preservation of scale imprints shows that the epidermal morphology of Synaptichnium differs from that of chirotheriids, and bears similarities to the lepidosauria. This observation is further proof of the primitive character of “chiroth‐eriid”; Synaptichnium, which is evident from both the pes anatomy and skin cover. Therefore the discovery of well‐preserved skin imprints at Gampenpass poses the question whether the classification of the ichnoge‐nus Synaptichnium should be revised.

Regarding Pendejo Cave: Response to Chrisman et al

Personal experience in Pendejo Cave and in the laboratory supporting the investigations there bears directly on claims for stratigraphic integrity forwarded by the authors of a report on ancient friction skin imprints. The published report is seriously incongruent with my notes and recollections. The statement that my work indicated no intrusions directly above the first recovery of imprints contradicts the fact that I spent my excavation time there clearing a large rodent burrow that penetrated to the layer in which the find was made. Abundant cultural remains of Holocene age in the uppermost strata of the cave constitute potential sources for anthropogenic items of all kinds that could have been intruded to great depths by burrowing fauna. Data supporting dismissal of such possibilities must be presented by the investigators on a firmer basis than given in their 1996 report.

Late Pleistocene Human Friction Skin Prints from Pendejo Cave, New Mexico

In the excavation of Pendejo Cave (FB 9366) near Orogrande, New Mexico, 16 friction skin imprints were found in five stratified zones on clay nodules, baked at over 120°C. After careful analysis, expert dermatoglyphologists determined that these imprints had positive primate characteristics. The imprints are probably of human origin, since no other primates are known to have existed in prehistoric New Mexico. Eight of the imprints occurred in three well-dated zones falling in the late Pleistocene. These zones have direct radiocarbon dates between 12,000 and 37,000 B.P. In addition to their association with radiocarbon determinations, the prints come from three of 24 stratified zones, intensively studied by geologists and pedologists, that are dated in sequence by 34 other radiocarbon determinations acquired from four different laboratories. The imprints are associated with a column of over 35,000 paleontological specimens and more than 15,000 botanical remains. These specimens indicate Pleistocene changes and supply evidence of human transportation and modification of various materials. The prints are also associated with artifacts, ecofacts, features of human construction, and human remains. The imprint specimens therefore provide evidence of Pleistocene human occupation in the New World.

Diffuse cutaneous leishmaniasis with visceral dissemination in an AIDS patient in Guadeloupe, West Indies

Barton, S. E.; Davies, S.; Schroeder, K.; Arthur, C.; Cazzard, B. C. Author Information

Paleomagnetism, Paleolatitudes, and Magnetic Overprinting on the North Slope, Alaska: ABSTRACT

Several paleomagnetic studies have been made in Arctic Alaska, by industry, by the U.S. Geological Survey, and by the University of Alaska. In general, the results available to the public have been disappointing--most samples of pre-Cretaceous rocks give very steep magnetic inclinations with respect to present horizontal. This has been generally interpreted in terms of a Cretaceous overprinting event. A study of the paleomagnetism of Cretaceous rocks from the North Slope shows that although the Cretaceous field was steeply inclined, it was not as steep as conventional paleogeographic reconstructions would indicate, and not as steep as the bulk of the apparently remagnetized older rocks. This finding leaves open the possibility that the steeper directions recorded by the older rocks are the result of regional tilt, or the result of a paleogeography that allowed an earlier, steeper remagnetizing field. The shallower inclinations seen in the Cretaceous sediments of the Nanushuk Group (Albian-Cenomanian based on the fossil record with one K-Ar age of 100 Ma from an ash parting) give paleolatitudes of about 75°N. The predicted paleolatitude based on North American paleogeographic reconstructions is 80-85°N. Circumstantial evidence that the paleolatitude was shallower than 80-85°N comes from the enormous biogenic productivity needed to form the extensive coal deposits of the Nanushuk Group. Lower paleolatitudes also may be needed to explain the apparent existence of broad-leaved evergreens and the recently reported dinosaur tracks and skin imprints in the Nanushuk Group. End_of_Article - Last_Page 680------------

Skin Imprints in Cutaneous Lymphoblastoma

Skin Imprints in Cutaneous Lymphoblastoma