Fingerling size Salmo gairdneri, Oncorhynchus kisutch, Notemigonus crysoleucas, Cyprinus carpio, and Ictalurus punctatus were exposed in the laboratory three times daily for up to seven days to pulses of either free chlorine or monochloramine. This regime simulated conditions often encountered in the outfall of steam electric generating plants which chlorinate intermittently. LC50's, LT50's and response isopleths giving various percentage mortalities, were computed from the bioassays. S. gairdneri, O. kisutch, and I. punctatus were the most sensitive to both types of chlorine. C. carpio were most resistant and the N. crysoleucas were intermediate in sensitivity. Temperature had relatively little effect on the toxicity of intermittent chlorine to the species tested. In this type of test regime, free chlorine was three to fourteen-fold more toxic (depending on the species) than monochloramine. Water quality criteria for the protection of fish should, in the future, take this differential toxicity into consideration.