Regulation Of Size Research Articles

A mechanical transition from tension to buckling underlies the jigsaw puzzle shape morphogenesis of histoblasts in the Drosophila epidermis.

The polygonal shape of cells in proliferating epithelia is a result of the tensile forces of the cytoskeletal cortex and packing geometry set by the cell cycle. In the larval Drosophila epidermis, two cell populations, histoblasts and larval epithelial cells, compete for space as they grow on a limited body surface. They do so in the absence of cell divisions. We report a striking morphological transition of histoblasts during larval development, where they change from a tensed network configuration with straight cell outlines at the level of adherens junctions to a highly folded morphology. The apical surface of histoblasts shrinks while their growing adherens junctions fold, forming deep lobules. Volume increase of growing histoblasts is accommodated basally, compensating for the shrinking apical area. The folded geometry of apical junctions resembles elastic buckling, and we show that the imbalance between the shrinkage of the apical domain of histoblasts and the continuous growth of junctions triggers buckling. Our model is supported by laser dissections and optical tweezer experiments together with computer simulations. Our analysis pinpoints the ability of histoblasts to store mechanical energy to a much greater extent than most other epithelial cell types investigated so far, while retaining the ability to dissipate stress on the hours time scale. Finally, we propose a possible mechanism for size regulation of histoblast apical size through the lateral pressure of the epidermis, driven by the growth of cells on a limited surface. Buckling effectively compacts histoblasts at their apical plane and may serve to avoid physical harm to these adult epidermis precursors during larval life. Our work indicates that in growing nondividing cells, compressive forces, instead of tension, may drive cell morphology.

A highly reproducible and efficient method for retinal organoid differentiation from human pluripotent stem cells

Human pluripotent stem cell (hPSC)-derived retinal organoids are three-dimensional cellular aggregates that differentiate and self-organize to closely mimic the spatial and temporal patterning of the developing human retina. Retinal organoid models serve as reliable tools for studying human retinogenesis, yet limitations in the efficiency and reproducibility of current retinal organoid differentiation protocols have reduced the use of these models for more high-throughput applications such as disease modeling and drug screening. To address these shortcomings, the current study aimed to standardize prior differentiation protocols to yield a highly reproducible and efficient method for generating retinal organoids. Results demonstrated that through regulation of organoid size and shape using quick reaggregation methods, retinal organoids were highly reproducible compared to more traditional methods. Additionally, the timed activation of BMP signaling within developing cells generated pure populations of retinal organoids at 100% efficiency from multiple widely used cell lines, with the default forebrain fate resulting from the inhibition of BMP signaling. Furthermore, given the ability to direct retinal or forebrain fates at complete purity, mRNA-seq analyses were then utilized to identify some of the earliest transcriptional changes that occur during the specification of these two lineages from a common progenitor. These improved methods also yielded retinal organoids with expedited differentiation timelines when compared to traditional methods. Taken together, the results of this study demonstrate the development of a highly reproducible and minimally variable method for generating retinal organoids suitable for analyzing the earliest stages of human retinal cell fate specification.

Overexpression of Solanum lycopersicum DDB1 interacting protein 4 (SlDDI4) extends tomato shelf life by methylation-mediated suppression of softening-related genes expression

Prolonging the fruit storage period is strategically important due to the limitation of postharvest life determined by excessive softening. Efforts have been made to alleviate the issue of fruit softening in transgenic fruit by suppressing genes that encode cell wall-degrading proteins. However, individual genes related to the degradation of cell walls have limited ability, resulting in minimal success. SET domain-containing protein (SET protein) is widely expressed in most plant organs and crucial in plant growth and development. A tomato SET protein was previously identified as SlDDI4 (Solanum lycopersicum DDB1 interacting protein 4) with a H3K9 methyltransferase activity involving cell proliferation and organ size regulation. In this study, we demonstrated that SlDDI4 overexpression (SlDDI4-OE) promotes fruit firmness in addition to increased fruit size. Further experimental investigations revealed that the increased expression of SlDDI4 inhibited cellulase and pectinase activities, consequently increasing the proportion of cellulose and insoluble pectin. RNA-seq profiling indicated the genes involved in cell wall metabolism in SlDDI4-OE fruit were significantly down-regulated. DNA methylation and McrBC-PCR analysis at the SlCEL2, SlPG2, and SlEXP12 loci revealed that their down-regulation was associated with the elevated methylation level in the individual promoter regions. Furthermore, up-regulation of SlDDI4 generated fruit with increased lycopene content and prolonging shelf-life by approximately 20 d without adverse impacts on the flavor indicators. Thus, our results suggest that manipulation of SlDDI4 expression enables broad favorable effects on postharvest life as well as fruit size and quality through chromatin remodeling.

Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion.

Smads and their transcription factor partners mediate the transcriptional responses of target cells to secreted ligands of the Transforming Growth Factor-β (TGF-β) family, including those of the conserved bone morphogenetic protein (BMP) family, yet only a small number of direct target genes have been well characterized. In C. elegans, the BMP2/4 ortholog DBL-1 regulates multiple biological functions, including body size, via a canonical receptor-Smad signaling cascade. Here, we identify functional binding sites for SMA-3/Smad and its transcriptional partner SMA-9/Schnurri based on ChIP-seq peaks (identified by modEncode) and expression differences of nearby genes identified from RNA-seq analysis of corresponding mutants. We found that SMA-3 and SMA-9 have both overlapping and unique target genes. At a genome-wide scale, SMA-3/Smad acts as a transcriptional activator, whereas SMA-9/Schnurri direct targets include both activated and repressed genes. Mutations in sma-9 partially suppress the small body size phenotype of sma-3, suggesting some level of antagonism between these factors and challenging the prevailing model for Schnurri function. A functional analysis of target genes revealed a novel role in body size for genes involved in one-carbon metabolism and in the endoplasmic reticulum (ER) secretory pathway, including the disulfide reductase dpy-11. Our findings indicate that Smads and SMA-9/Schnurri have previously unappreciated complex genetic and genomic regulatory interactions that in turn regulate the secretion of extracellular components like collagen into the cuticle to mediate body size regulation.

Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton.

Plants have evolved diverse defense mechanisms encompassing physical and chemical barriers. Cotton pigment glands are known for containing various defense metabolites, but the precise regulation of gland size to modulate defense compound levels remains enigmatic. Here, it is discovered that the VQ domain-containing protein JAVL negatively regulates pigment gland size and the biosynthesis of defense compounds, while the MYC2-like transcription factor GoPGF has the opposite effect. Notably, GoPGF directly activates the expression of JAVL, whereas JAVL suppresses GoPGF transcription, establishing a negative feedback loop that maintains the expression homeostasis between GoPGF and JAVL. Furthermore, it is observed that JAVL negatively regulates jasmonate levels by inhibiting the expression of jasmonate biosynthetic genes and interacting with GoPGF to attenuate its activation effects, thereby maintaining homeostatic regulation of jasmonate levels. The increased expression ratio of GoPGF to JAVL leads to enlarged pigment glands and elevated jasmonates and defense compounds, enhancing insect and pathogen resistance in cotton. These findings unveil a new mechanism for regulating gland size and secondary metabolites biosynthesis, providing innovative strategies for strengthening plant defense.

Comparative transcriptome analysis of vegetable soybean grain discloses genes essential for grain quality

BackgroundVegetable soybean is an important vegetable crop in world. Seed size and soluble sugar content are considered crucial indicators of quality in vegetable soybean, and there is a lack of clarity on the molecular basis of grain quality in vegetable soybean.ResultsIn this context, we performed a comprehensive comparative transcriptome analysis of seeds between a high-sucrose content and large-grain variety (Zhenong 6, ZN6) and a low-sucrose content and small-grain variety (Williams 82, W82) at three developmental stages, i.e. stage R5 (Beginning Seed), stage R6 (Full Seed), and stage R7 (Beginning Maturity). The transcriptome analysis showed that 17,107 and 13,571 differentially expressed genes (DEGs) were identified in ZN6 at R6 (vs. R5) and R7 (vs. R6), respectively, whereas 16,203 and 16,032 were detected in W82. Gene expression pattern and DEGs functional enrichment proposed genotype-specific biological processes during seed development. The genes participating in soluble sugar biosynthesis such as FKGP were overexpressed in ZN6, whereas those responsible for lipid and protein metabolism such as ALDH3 were more enhanced in W82, exhibiting different dry material accumulation between two genotypes. Furthermore, hormone-associated transcriptional factors involved in seed size regulation such as BEH4 were overrepresented in ZN6, exhibiting different seed size regulation processes between two genotypes.ConclusionsHerein, we not only discovered the differential expression of genes encoding metabolic enzymes involved in seed composition, but also identified a type of hormone-associated transcriptional factors overexpressed in ZN6, which may regulate seed size and soluble content. This study provides new insights into the underlying causes of differences in the soybean metabolites and appearance, and suggests that genetic data can be used to improve its appearance and textural quality.

Regulation of pupil size in natural vision across the human lifespan.

Vision is mediated by light passing through the pupil, which changes in diameter from approximately 2 to 8 mm between bright and dark illumination. With age, mean pupil size declines. In laboratory experiments, factors affecting pupil size can be experimentally controlled. How the pupil reflects the change in retinal input from the visual environment under natural viewing conditions is unclear. We address this question in a field experiment (N = 83, 43 female, 18-87 years) using a custom-made wearable video-based eye tracker with a spectroradiometer measuring near-corneal spectral irradiance. Participants moved in and between indoor and outdoor environments varying in spectrum and engaged in a range of everyday tasks. Our data confirm that light-adapted pupil size is determined by light level, with a better model fit of melanopic over photopic units, and that it decreased with increasing age, yielding steeper slopes at lower light levels. We found no indication that sex, iris colour or reported caffeine consumption affects pupil size. Our exploratory results point to a role of photoreceptor integration in controlling steady-state pupil size. The data provide evidence for considering age in personalized lighting solutions and against the use of photopic illuminance alone to assess the impact of real-world lighting conditions.

Size prediction of drug-loaded Polymeric (PLGA) microparticles prepared by microfluidics

Drug-loaded poly (lactic-co-glycolic acid) (PLGA) microparticles with ex-act control over size and homogeneity were created using a glass microfluidic cross-junction droplet generator. A numerical simulation based on the volume-of-fluid (VOF) method is used to study the behavior of two immiscible fluids that are injected into the microdevice: hydrophobic drug and (PLGA) in dichloromethane (DCM) serving as the dispersed phase and aqueous solution of poly (vinyl alcohol) (PVA) as the continuous phase. The simulation predicts the created emulsion droplet size regulation by adjusting the phase's flow rate. In a procedure known as droplet shrinking, dichloromethane extraction, and evaporation took place to further transform the monodisperse emulsion into PLGA microparticles. A numerical relationship is also given for the variations in the volume of droplets during shrinkage. The numerical results were consistent with the experiments. To further illustrate this technique's ability to manufacture designed microspheres with the least amount of repeated experiments, as well as the importance of the droplet microfluidic approach in terms of regulating diameter and monodispersity, opioid antagonist naltrexone is loaded in PLGA microspheres. According to the findings, integrating a versatile microfluidic approach with numerical methodologies enables the development of more reliable and reproducible drug delivery systems.

Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder

Under ideal conditions, Escherichia coli cells divide after adding a fixed cell size, a strategy known as the adder. This concept applies to various microbes and is often explained as the division that occurs after a certain number of stages, associated with the accumulation of precursor proteins at a rate proportional to cell size. However, under poor media conditions, E. coli cells exhibit a different size regulation. They are smaller and follow a sizer-like division strategy where the added size is inversely proportional to the size at birth. We explore three potential causes for this deviation: degradation of the precursor protein and two models where the propensity for accumulation depends on the cell size: a nonlinear accumulation rate, and accumulation starting at a threshold size termed the commitment size. These models fit the mean trends but predict different distributions given the birth size. To quantify the precision of the models to explain the data, we used the Akaike information criterion and compared them to open datasets of slow-growing E. coli cells in different media. We found that none of the models alone can consistently explain the data. However, the degradation model better explains the division strategy when cells are larger, whereas size-related models (power-law and commitment size) account for smaller cells. Our methodology proposes a data-based method in which different mechanisms can be tested systematically.

Optical second harmonic generation of tunable spherical quantum dots under Inversely Quadratic Hellmann Plus Inversely Quadratic Potential

Herein, we employ the effective mass approximation and the iterative method to investigate the Second harmonic generation (SHG) coefficient of a spherical quantum dot (QD) system with Inversely Quadratic Hellmann Plus Inversely Quadratic Potential (IQHIQP), considering the regulation of the quantum size, confinement potential depth and external environment. The numerical simulation results show that the SHG coefficient is highly reliant on the tunable factor, which can affect the energy level interval and dipole moment elements. Interestingly, the effect of temperature on the SHG coefficient in terms of peak location and size is consistent with the quantum dot radius but opposite to the hydrostatic pressure. Therefore, it is necessary to pay more attention to the influence of internal and external parameters on nonlinear optical effects, and apply the theory to practical experiments and the manufacture of optoelectronic devices.

Polycrystalline secondary particle size regulation boosts the cycle performance of ultra-high-nickel layered oxide cathode materials

The ultra-high-nickel layered oxide cathodes of LiNixCoyMn1-x-yO2 (NCM, and x ≥ 0.9) have been considered promising candidates to meet the growing demand for high-energy-density lithium-ion batteries (LIBs) due to their high specific capacity. However, their capacity retention tends to be low in practical applications due to the instability of their structure during charge and discharge cycles. Herein, a strategy for regulating secondary particle size is put forward to alleviate the crystal volume change and suppress the H2-H3 phase transitions, ultimately stabilizing the structure against microcrack development and improving the cycle performance of the ultra-high-nickel layered oxide cathodes. The results show that LiNi0.90Co0.05Mn0.05O2 (NCM90) with a D50 of 8.6 μm (NCM90–9) exhibits the optimum electrochemical performance compared to its counterparts, which delivers a high cycling stability (91.86 % capacity retention after 200 cycles at 0.5 C) and a good rate performance (127.3 mAh·g-1 at 6 C). The superior performance of NCM90–9 is attributed to the suppression of microcracks within secondary particles and parasitic reactions with the electrolyte, which are determined by its secondary particle size. This result provides valuable guidelines for the development of ultra-high-nickel layered oxide cathodes.

Amphiphilic N-halamine copolymer antibacterial nanoparticles with hydrogen-donor and hydrogen-acceptor

Bacterial contamination in water bodies is potentially transmissible to humans, making water bodies a major pathway of bacterial transmission. However, existing hydantoin copolymer nanoparticles did not achieve the regulation of nanoparticle dispersion and particle size by adjusting the ratio of hydrogen-donors and hydrogen-acceptors. To tackle this issue, amphiphilic hydantoin copolymer nanoparticles (P(VBDH-BA-NVP)NPs) were synthesized by modulating hydrogen-donors and hydrogen-acceptors using emulsion polymerization, and amphiphilic N-halamine copolymer nanoparticles (P(ClV-BA-NVP)NPs) were subsequently prepared via chlorination treatment. Impressively, nanoparticle dispersion and particle size were modulated by adjusting the ratio of hydrogen-donors and hydrogen-acceptors. With the increase of nanoparticle dispersion and the decrease of particle size, the active chlorine content was gradually enlarged, and the antibacterial performance was progressively enhanced against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). More importantly, amphiphilic N-halamine copolymer nanoparticles with two-dimensional dispersing (2DD-P(ClV-BA-NVP)NPs) exhibited excellent antibacterial properties, which benefited from the dual coordination of direct contact killing and indirect release killing in aqueous medium. Through designing P(VBDH-BA-NVP)NPs with hydrogen bond interactions, this approach aims to achieve the constitutive relationship between the effective exposure of active sites and the antibacterial effect.

Piezoelectric-synchronized step emulsification for facile generation of microdroplet library with fluid-independent drop size regulation regime

Microdroplets play a crucial role in various applications based on microfluidics, including biomedicine, materials synthesis, and analytical chemistry. However, there is still an urgent need for a microdroplet preparation method that is simple in design, precise in droplet size control and meanwhile independent of the properties of the fluid. Here, we present a piezoelectric-synchronized step emulsification method. The drop formation process can be synchronized by the introduced periodic vibration via the piezoelectric element, which occurs in a specific frequency range determined by a dimensionless parameter. The competing between the step emulsification regime and the piezoelectric-triggered local flow rate fluctuation leads to various microscopic flow behavior. A novel instantaneous jetting regime is found to be responsible for the extended achievable drop size range. By combining the piezoelectric step emulsification and the XY stage control, we achieve facile production of microdroplet and hydrogel microsphere library in well plates, which is promising to be integrated as a low-cost drop-generating technique into bioassay instruments.

The basement membrane regulates the cellular localization and the cytoplasmic interactome of Yes-Associated Protein (YAP) in mammary epithelial cells.

The Hippo pathway, a signaling cascade involved in the regulation of organ size and several other processes, acts as a conduit between extracellular matrix (ECM) cues and cellular responses. We asked whether the basement membrane (BM),a specialized ECM component known to induce quiescence and differentiation in mammary epithelial cells, would regulate the localization, activity, and interactome of YAP, a Hippo pathway effector. To address this question, we used a broad range of experimental approaches, including 2D and 3D cultures of both mouse and human mammary epithelial cells, as well as the developing mouse mammary gland. In contrast to malignant cells, nontumoral cells cultured with a reconstituted BM (rBM)displayed higher concentrations of YAP in the cytoplasm. Incidentally, when in the nucleus of rBM-treated cells, YAP resided preferentially at the nuclear periphery. In agreement with our cell culture experiments, YAP exhibited cytoplasmic predominance in ductal cells of developing mammary epithelia, where a denser BM is found. Conversely, terminal end bud (TEB)cells with a thinner BM displayed higher nucleus-to-cytoplasm ratios of YAP. Bioinformatic analysis revealed that genes regulated by YAP were overrepresented in the transcriptomes of microdissected TEBs. Consistently, mouse epithelial cells exposed to the rBM expressed lower levels of YAP-regulated genes, although the protein level of YAP and Hippo components were slightly altered by the treatment. Mass spectrometry analysis identified a differential set of proteins interacting with YAP in cytoplasmic fractions of mouse epithelial cells in the absence or presence of rBM. In untreated cells, YAP interactants were enriched in processes related to ubiquitin-mediated proteolysis, whereas in cells exposed to rBM YAP interactants were mainly key proteins related to amino acid, amino sugar, and carbohydrate metabolism. Collectively, we unraveled that the BM induces YAP translocation or retention in the cytoplasm of nontumoral epithelial cells and that in the cytoplasm YAP seems to undertake novel functions in metabolic pathways.

Pleiotropic phenotypic effects of the TaCYP78A family on multiple yield-related traits in wheat.

Increasing crop yield depends on selecting and utilizing pleiotropic genes/alleles to improve multiple yield-related traits (YRTs) during crop breeding. However, synergistic improvement of YRTs is challenging due to the trade-offs between YRTs in breeding practices. Here, the favourable haplotypes of the TaCYP78A family are identified by analysing allelic variations in 1571 wheat accessions worldwide, demonstrating the selection and utilization of pleiotropic genes to improve yield and related traits during wheat breeding. The TaCYP78A family members, including TaCYP78A3, TaCYP78A5, TaCYP78A16, and TaCYP78A17, are organ size regulators expressed in multiple organs, and their allelic variations associated with various YRTs. However, due to the trade-offs between YRTs, knockdown or overexpression of TaCYP78A family members does not directly increase yield. Favourable haplotypes of the TaCYP78A family, namely A3/5/16/17Ap-Hap II, optimize the expression levels of TaCYP78A3/5/16/17-A across different wheat organs to overcome trade-offs and improve multiple YRTs. Different favourable haplotypes have both complementary and specific functions in improving YRTs, and their aggregation in cultivars under strong artificial selection greatly increase yield, even under various planting environments and densities. These findings provide new support and valuable genetic resources for molecular breeding of wheat and other crops in the era of Breeding 4.0.

Tailoring the deformation mechanisms in a Ni-Co-based superalloy by controlling γ′ precipitate size

The role of micro-twins in the high-temperature deformation of superalloys is crucial and influenced by precipitates and deformation conditions. This study investigates the deformation mechanisms in a Ni-Co-based superalloy with γ′ precipitates varying from 31 to 122 nm under two compression conditions at 760 °C. The deformation mechanism exhibits a close correlation with the size of γ′ precipitates under 5 % stain, while the formation of micro-twins is relatively limited. Microtwinning becomes the predominant deformation mode when compressed to 20 %. The micro-twins reach the peak intensity for the alloy with 47 nm-sized γ′ precipitate. The optimizing of γ′ precipitate size can significantly enhance the formation of micro-twins in Ni-Co-based superalloys. This strategy of modulating the deformation mechanism through precipitate size regulation offers valuable insights for the design of advanced alloys.

Optimizing fouling mitigation in membrane distillation by controlled microbubble size and concentration

Fouling control strategies play a crucial role in membrane distillation (MD) process, primarily due to the significant limitation of fouling during the scaling-up process. The utilization of microbubbles (MBs) aeration has exhibited promising potential in mitigating membrane fouling when treating high salinity wastewater. Aeration can introduce gas-liquid two-phase flow on the membrane surface, thereby increasing the membrane surface shear force. The mechanism of aeration technology for controlling fouling includes the following points: introduction of gas-liquid two-phase flow; physical substitution of concentration polarization layer; pressure pulses caused by bubbles flowing through the membrane surface and increasement of the cross flow velocity. This research aims to optimizing the effect of air MBs with different concentrations and sizes introduced in the feed side on membrane fouling control, we creatively used the method of releasing pressure gradiently to achieve size regulation of MBs within a certain range for the first time, firstly regulated the generation of MBs by manipulating gas flow rate and release pressure. Under the optimal condition of 40 mL/min and 0.6 MPa, it will yield concentration of 1.58 × 104/mL and D50 Sauter size of 64.69 μm MBs with pure water permeance flux of 12.32 kg/(m2·h). It was observed that higher gas flow rates primarily increased MBs concentration, while lower pressures predominantly reduced MBs size through the method of inline imaging. Experimental results indicated that introduction of MBs can slightly improve the temperature polarization coefficient and mass transfer coefficient under different operating modes. Consequently, further investigations focused on the influence of MBs characteristics on different fouling control. All conditions reach 99 % rejection for inorganic salt and HA. This study provided empirical evidence that higher concentration and larger size of MBs intensify the gas-liquid two-phase flow disruption and exhibit a better shear effect on scaling phenomena, which yield advantageous outcomes in terms of augmenting permeance flux and water vapor production, with permeance flux increased from 40.12 % to 55.42 % for inorganic fouling and increased from 68.35 % to 80.15 % for inorganic and HA fouling. These results were confirmed via membrane surface using scanning electron microscopy and energy dispersive spectroscopy. In summary, this research aided in identifying appropriate operating conditions for mitigating inorganic and composite scaling by implementing air-infused MBs in direct contact membrane distillation (DCMD), which provides scientific and technical guidance for controlling fouling in MD in practical applications.

IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells.

IL-2Rα knock out (KO) mice have been instrumental to discovering the immunoregulatory properties of IL-2Rα. While initially thought of only as a stimulatory cytokine, IL-2 and IL-2Rα KO mice revealed that this cytokine-receptor system controls immune responses through restimulation-induced cell death and by promoting the survival of T regulatory cells. Although described mostly in the context of lymphocytes, recent studies by our laboratory showed that IL-2R is expressed in smooth muscle cells. Given this finding, we sought to use IL-2Rα KO to determine the function of this receptor in vascular smooth muscle cells. Surprisingly, we found that IL-2Rα KO vascular smooth muscle cells had detectable IL-2Rα. We used multiple gene and protein-based methods to determine why IL-2Rα KO vascular smooth muscle cells exhibited IL-2Rα protein. These methods included: genomic sequencing, assessing cells and tissues for evidence of maternal microchimerism, and determining the half-life of IL-2Rα protein. Our studies demonstrated the following: (1) in addition to the cell surface, IL-2Rα is localized to the nucleus; (2) the genetic deletion of IL-2Rα is intact in IL-2Rα KO mice; (3) both IL-2Rα KO and WT tissues show evidence of maternal microchimerism, the likely source of IL-2Rα (4) IL-2Rα is transmitted between cells; (5) IL-2Rα has a long half-life; and (6) nuclear IL-2Rα contributes to the regulation of cell proliferation and size. Our findings suggest that the phenotype of complete IL-2Rα loss is more severe than demonstrated by IL-2Rα KO mice, and that IL-2Rα plays a here-to-fore unrecognized role in regulating cell proliferation in non-lymphoid cells.

Comprehensive identification of maize ZmE2F transcription factors and the positive role of ZmE2F6 in response to drought stress

BackgroundThe early 2 factor (E2F) family is characterized as a kind of transcription factor that plays an important role in cell division, DNA damage repair, and cell size regulation. However, its stress response has not been well revealed.ResultsIn this study, ZmE2F members were comprehensively identified in the maize genome, and 21 ZmE2F genes were identified, including eight E2F subclade members, seven DEL subfamily genes, and six DP genes. All ZmE2F proteins possessed the DNA-binding domain (DBD) characterized by conserved motif 1 with the RRIYD sequence. The ZmE2F genes were unevenly distributed on eight maize chromosomes, showed diversity in gene structure, expanded by gene duplication, and contained abundant stress-responsive elements in their promoter regions. Subsequently, the ZmE2F6 gene was cloned and functionally verified in drought response. The results showed that the ZmE2F6 protein interacted with ZmPP2C26, localized in the nucleus, and responded to drought treatment. The overexpression of ZmE2F6 enhanced drought tolerance in transgenic Arabidopsis with longer root length, higher survival rate, and biomass by upregulating stress-related gene transcription.ConclusionsThis study provides novel insights into a greater understanding and functional study of the E2F family in the stress response.

Seasonal changes in ecosystem health and their spatial relationship with landscape structure in china's Loess plateau

Environmental managers have sought to optimize landscape structure to maintain regional ecosystem health (EH). However, we still lack a full understanding of the effects of changing landscape structure on EH, especially in terms of shorter time scales (e.g., monthly or seasonal scales) and spatial heterogeneity. To address this gap, this study used the vigor-organization-resilience-services (VORS) model to assess the annual and seasonal variation of EH in the Loess Plateau (LP) during 2000–2015, and then applied the geographically weighted regression (GWR) model to investigate the spatial effects of different aspects (i.e., landscape composition and configuration) of the landscape structure change on EH over time. Our results showed that the EH level in the LP from 2000 to 2015 displayed temporal non-stationarity at annual and seasonal scales, and a spatial pattern of decreasing from southeast to northwest. The effects of landscape structure on EH had obvious spatial and temporal heterogeneity, depending on the local landscape composition and configuration. In general, landscape configuration exerted a greater impact on EH than landscape composition apart from Shannon’s diversity index and percentage of forestland area, but the intensity and direction of the impact displayed higher temporally instability and therefore should receive more attention. Furthermore, regulation of the dominant patch size, landscape connectivity and forest spatial spacing is the optimal choice for regional ecosystem management. The results suggest that EH responses to changes in landscape structure in the LP vary by time and place, and that the integrating temporal variation and spatial characteristics can help decision-makers make precise spatial arrangements and temporal adjustments to keep the negative ecological impacts within reasonable limits.