Wound Size Research Articles

Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms.

Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2mL of 250 and 500mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-β1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.

A clinical study of the local injection of a freshly manufactured 35 kDa hyaluronan fragment for treating chronic wounds.

This study manufactured a 35 kDa hyaluronan fragment (HA35) by enzymatically degrading high-molecular-weight HA using hyaluronidase PH20 derived from bovine testis. The research then examined the therapeutic efficacy of locally administered, tissue-permeable HA35 in alleviating chronic wounds and their associated neuropathic pain. For 20 patients with nonhealing wounds and associated pain lasting over three months, 100 mg of HA35 was injected daily into the healthy skin surrounding the chronic wound for 10 days. Self-assessmentsbefore and after treatment indicated that HA35 significantly enhanced wound healing. This was evidenced by the formation of fresh granulation tissue on the wounds (p < 0.0001); reduceddarkness, redness, dryness, and damage in the skin surrounding the wounds (p < 0.0001), and a decrease in wound size (p < 0.001). Remarkably, HA35 injections alleviated pain associated with chronic wounds within 24 hours (p < 0.0001). It can be concluded that the low-molecular-weight hyaluronan fragment HA35 potentially enhancesthe immune response and angiogenesis during wound healing.

Impact of foot ulcer-related factors on quality of life in patients with diabetes: Prospective observational study.

Quality of life (QOL) may be impacted by foot ulcer-related factors, with prevention of diabetes-related foot ulcers or more effective early healing helping to improve overall patient QOL. This study, which examined the relationship between foot ulcer-related factors and QOL in patients with diabetes, was conducted as a secondary analysis of a prospective observational study entitled: "Factors associated with the discontinuation of wound care specialist clinic visits in patients with diabetic foot ulcers". We investigated EQ-5D-5L, patient characteristics and foot ulcer-related factors of 73 patients with diabetes-related foot ulcers who visited one wound clinic in Indonesia between August 2020 and February 2021. Results showed that the mean health utility was 0.294 ± 0.371. Factors selected for the multiple regression analysis included inflammation/infection of DMIST, first-ever foot ulcer, and size of DMIST. First-ever foot ulcer (β = 0.309, p = 0.003) and size of DMIST (β = -0.316, p = 0.015) were significantly associated with the health utility (p < 0.001). Significant improvement in the health utility of 15 patients was observed when the ulcer healed (Wilcoxon signed-rank sum test, p = 0.001). In conclusion, not only ulcer severity but also the first-ever foot ulcer itself affected the QOL in patients with diabetes. These results suggest there will be a greater impact on the QOL of patients who develop diabetes-related foot ulcers for the first time, along with the importance of prevention and early healing, through early infection control and wound size reduction.

Management of post-Mohs surgical wounds with a hypothermically stored amniotic membrane: a case series.

The aim of this case series is to present an alternative approach to managing post-Mohs Micrographic Surgery (Mohs) wounds with hypothermically stored amniotic membrane (HSAM). A case series of patients with post-Mohs wounds is presented, with four patients referred for hard-to-heal wounds following a Mohs procedure that was performed 1-3 months previously. All wounds underwent weekly assessment, debridement, and application of HSAM and secondary dressings. Treatment also included management of bioburden, proper skin care and compression therapy for lower extremity wounds. This case series of seven wounds consisted of four females and three males with a mean age of 87.6 years. Mean wound size at first application of HSAM was 1.34±1.20cm2. All wounds closed, with an average time to wound closure of 43.7±27.1 days. Patients received an average of 4.6±2.5 HSAM applications. The four post-Mohs wounds with a history of being hard-to-heal had an average time to wound closure of 35.5±16.3 days, with an average duration of 86.5±32.4 days prior to the first HSAM application. The results of this case series suggest that use of HSAM may provide an alternative approach to managing post-Mohs wounds. In addition, these findings suggest that HSAM may be of greatest benefit when applied early after Mohs surgery.

Use of Cold Plasma in the Treatment of Infected Wounds

The fact that chronic and complex wounds are a serious problem, both for those affected and for the health care system, has been known for decades. Most chronic wounds can be healed through targeted treatment of the cause of the wound and optimal wound care. This includes not only phase-adapted modern wound care, but also, in particular, preparation for surgical coverage. The correct preparation of the wound bed, also known as conditioning, plays an important role in this. In recent years, the use of Cold Atmospheric Plasma (CAP) has emerged as a promising new option. Here, the wound is regularly treated with a partially ionized gas. According to studies, this active gas mixture has antimicrobial properties and promotes wound healing by activating cell regenerative processes. To test the efficacy of cold plasma therapy, for complex hard to heel wound with difficult conditions, 40 patients with 41 wounds were included in a retrospective, multicenter observational trial in Italy. The wounds were complex wounds of different genesis that had been present for at least 2 months (&gt; 60 days) were treated with cold atmospheric plasma, generated with the CE approved handheld plasma device plasma care®. The treatment, as an add on to standard wound therapy, was performed once a week and for 1 Minute per treated wound area for an intervention period of 4 weeks. In the intervention period, a total of 41 wounds from 40 patients were treated and measured. Two patients left the trial, the others showed an average wound size reduction of 28% within 30 days. Of all wounds, two were completely closed at the end and 10 wounds even achieved a reduction of at least 40%. Analyzed by initial wound size, 43% of wounds smaller than 18 cm² reduction of 40 %. No improvement or worsening of the wound size was only observed in five wounds. The observational trial showed a reduction in the bacterial load and accelerated wound healing. In addition to the bacterial load, improved wound bed conditioning was also demonstrated. Cold plasma therapy is well suited for locally targeted application to promote wound healing. It is an uncomplicated and quickly applicable method with no known side effects or risks at present.

Assessing the Residual Stand Damage after Thinning with Different Levels of Mechanization

Thinning is a silvicultural process in which trees are both harvested selectively and systematically removed from a stand to enhance forest management and ecosystem dynamics. However, this practice is challenged by the mechanical damage to residual trees, and the nature of this damage, particularly on stand damage during mechanized row-thinning and manual selective thinning, in South Korea is unknown. Therefore, objectives of this study were to compare stand damage levels and wound characteristics between three different thinning operations: manual selective, manual row-, and mechanized row-thinning. After thinning, 12%, 15%, and 10% of the residual damaged trees were observed in manual selective, manual row-, and mechanized row-thinning, respectively. Both types of row-thinning predominantly demonstrated damages at the stem and butt region, whereas in manual selective thinning, the most of the damages occurred on the roots. Manual selective thinning exhibited a slightly larger average wound size (207 cm2) compared to mechanized row- (181 cm2) and manual row-thinning (165 cm2). The wound sizes on the stem in mechanized row-thinning were significantly higher than manual selective (p &lt; 0.05). These results may be helpful in understanding exposure to damage among different thinning types and in managing its occurrence in future practices.

Comparison of Fat Graft in Post-Burn Scars versus Platelet-Rich Plasma Regarding Scar Quality and Healing

Post-burn scars occur when burns damage the skin leading inevitable injury. Various techniques are applied for treatments and reduction so f these scares. Objective: To compare the effect of Platelet Rich Plasma (PRP) and fat graft in post-burn scars on the outcome of the healing process and quality of chronic wounds. Methods: One hundred cases were equally divided in group I (platelet-rich plasma) and group II (fat graft), age between 20-60 years were enrolled. The patients were those who were having post traumatic or and post burn chronic wounds which were within the duration of ≥3 months. A 5cc blood was withdrawn from each patient. Fat graft and platelet rich plasma were prepared, applied and compared for up to 12 weeks for their wound size, healing rate and scar quality. Results: There was no difference win age or gender within groups with a mean age of 43.46 and 44.36 years in group I and Group I. The comparative analysis within the original wound size and decrease in it within the 4 weeks’ time followed by 12 weeks’ time presented significant variance with both procedures bringing sufficient minimizing in the size, however a slight better result was presented in group II in comparison with Group I. Average healing rate was higher in group II than group I. Conclusions: Both procedures are efficient in terms of scar quality and healing of post burn scar. However, within the two groups the fat graft is more efficient and reliable with high healing time and rate.

Advancements in vacuum therapy for musculoskeletal wound care: A comprehensive analysis of chronic wounds and acute injuries

Musculoskeletal wounds are challenging to treat. Vacuum therapy (VT), based on the principle of applying negative pressure to a wound, has gained popularity as an adjunctive treatment modality for musculoskeletal wounds. Clinical studies have shown that VT improves wound bed preparation, reduces wound size, accelerates healing rates, and decreases time to wound closure. It is also associated with a lower risk of wound complications, such as infection, dehiscence, and seroma formation. The integration of VT into a comprehensive wound management approach has the potential to improve outcomes for patients with musculoskeletal wounds. This review provides a comprehensive overview of VT for musculoskeletal wound care. The review discusses the current practices, mechanism of action, benefits, risks, and future directions of VT. The review concludes that VT is a valuable tool for the treatment of musculoskeletal wounds, and that it is safe and effective for most patients.

Oxindoline Containing Thiazolidine‐4‐one Tethered Triazoles Act as Antimitotic Agents by Targeting Microtubule Dynamics

AbstractOxindoline containing thiazolidine‐4‐ones linked triazole hybrids (24 a–l) series were synthesized by multistep synthesis and tested for their cytotoxic activity against four human cancer cell lines. Significantly higher susceptibility was observed in liver (HepG2) cancer cells. Among them conjugates 24 c and 24 e exhibited promising cytotoxicity with IC50 values of 1.64 μM, and 2.07 μM respectively when compared to the standard 5‐fluorouracil (IC50=20.8 μM) against the HepG2 cell line. Later on, these compounds changed the morphology of HepG2 cells and significantly inhibited colony formation in HepG2 in a dose‐dependent manner. The scratch assay divulged that these compounds decreased wound size and inhibited cell migration. Interestingly, they induced the cell cycle arrest at the S phase. Moreover, the DCFDA assay indicated ROS production proportionate to compound dose and AO/Eb staining revealed increased apoptotic cell count with higher doses. The tubulin polymerization assay reveals that they inhibited the microtubules and acted as antimitotic agents. In‐silico results of these conjugates also support their tubulin inhibitory properties. Interestingly, these compounds have adequate ADME‐toxicity parameters. Based on free energy calculation results, it was observed that these inhibitors influence the conformational flexibility of the target tubulin protein.

Multimodal imaging device to comprehensively assess infection, oxygenation, and wound analytics-A pilot study.

Wound analytics, infection detection, and oxygenation measurement are the three critical prerequisites for appropriate wound care. Although devices that rapidly detect the above-mentioned parameters independently exist, there is no single point-of-care device that is enabled with all the three functionalities. Through this study, we are introducing and evaluating the performance of Illuminate Pro Max-a novel, rapid, hand-held non-contact, point-of-care multimodal imaging device that is equipped to measure the three wound assessment parameters. Here, a total of 60 diabetic foot ulcer patients were imaged using Illuminate Pro Max to detect bioburden and measure StO2 levels and wound dimensions (size and depth). The results were further evaluated against the current gold standard technique for each parameter, that is, culture test to detect bioburden, a transcutaneous oxygen pressure (TcPO2) measuring device-Perimed Periflux 5000 to measure oxygenation, and paper ruler to measure wound size. Culture tests reported 42 samples as infection-positive and 18 samples as infection-negative. On comparing with the culture report, the device showed 88% sensitivity and 86% PPV in detecting the bioburden. Wound dimensions (length and width) were comparable with the paper scale measurements. Wound depth was also reported by the device. The StO2 map generated by the device depicted the tissue oxygenation levels in various regions of the wound. In conclusion, this novel, comprehensive point-of-care multispectral imaging device can be an effective tool for rapid wound assessment which can help in prompt treatment.

Does Full-Mouth Disinfection Influence the Size of the Periodontal Inflammatory Burden and the Level of hsCRP?

To investigate the effect of full-mouth disinfection on the sizes of the periodontal wound and periodontal inflammatory burden and whether it leads to a decrease in C-reactive protein (CRP) levels. The study included 20 systemically healthy subjects (11 women and 9 men) 30 to 68 years old with localised or generalised periodontitis (stage III, grade C). The sizes of the periodontal wound and periodontal inflammatory burden were measured with the web application "Periodontalwound", which is based on measurements of average tooth cervices, as well as probing depths and bleeding on probing assessed at six sites around each tooth present in the oral cavity. The levels of hsCRP (high-sensitivity CRP) were measured with an immunochemical method. All three parameters were measured before initial treatment and 3 months after therapy. Full-mouth disinfection included removal of plaque and calculus with ultrasonic and hand instruments in one session. The results showed a statistically significant decrease in the size of the periodontal wound (p < 0.001), a statistically significant decrease in the size of periodontal inflammatory burden (p < 0.001), and a decrease in hsCRP levels 3 months after therapy. Full-mouth disinfection leads to a decrease in the periodontal wound and periodontal inflammatory burden size, as well as a decrease in the levels of hsCRP in patients with localised or generalised periodontitis (stage III, grade C).

Physical therapy in burn wound healing: Development of clinical prediction rules to identify the efficacy of pulsed electromagnetic therapy

Many studies have demonstrated the effect of pulsed electromagnetic therapy (PEMT) on wound healing. This study aimed to develop a clinical prediction rule (CPR) to assess PEMT's efficacy in burn healing, potentially enhancing treatment decisions and outcomes. It was a one-group intervention study with 46 patients (21 males, 25 females) aged 20 to 55 years, having partial-thickness burns in the first or second healing stage, and a total burned surface area (TBSA) over 15%. The intervention involved pulsed electromagnetic therapy (Fisioline, Italy) for up to six weeks, with 60-minute sessions at 12 Hz and 12 Gauss, three times per week. The statistical analysis was conducted using the SPSS. The study revealed a significant decline in wound surface area (WSA) post-intervention (p &lt; 0.05). A significant negative relationship was found between wound improvement and both age and total body surface area (TBSA) (p &lt; 0.05), while a significant positive relationship was observed between wound improvement and initial wound size (p &lt; 0.05). No significant relationship was found between wound improvement and wound stage (p &gt; 0.05). The study concluded that pulsed electromagnetic therapy significantly reduces wound surface area. Additionally, age, TBSA, and initial burn wound size are important predictors of the therapy's efficacy in treating burn wounds.

Efficacy of Amniotic and Chorionic Membrane in Facial Wound Healing: A Comparative Study.

Background Advancements in regenerative techniques have been utilized in placental amnion and chorion for a variety of purposes. Their ability to regenerate tissues has led to their usage in tissue engineering, wound healing, and other therapeutic applications. This study aims to evaluate and compare the efficacy of amnion and chorion in facial tissue wound healing. Methodology The study was an observational comparative study conducted in the Department of Oral and Maxillofacial Surgery, involving 20 participants divided into two groups (Group I and Group II).Study groups were selected according to the inclusion and exclusion criteria. A dehydrated human amnion/ chorion membrane was applied to the affected site of each group respectively. Its efficacy in wound healing was analyzed in the first, third, seventhday, and secondweek. Statistical analysis was done using SPSS software(IBM Corp., Armonk, NY). Results Patients treated with amnion membrane showed a decrease in wound size and the wound was completely healed by secondweek with mean scores of wound sizes of 0.00whereas the wound remained unhealed by secondweek with mean of 1.70to those treated with chorion membrane. Conclusion Amnion showed superior efficacy in wound healing at two-week intervals when compared to the chorion. Hence, this could be used in regenerative medicine as a graft to induce healing in facial wounds.

Studying The Interaction of Laser with Human Tissue

A laser is amplified light beam to emit radiation. these rays are used in surgery by accurately projecting laser light to remove diseased tissue or bleed blood vessels by heating the target cells until they explode. The laser is used medically under objective or complete anesthesia. There are many types of lasers such as carbon dioxide laser, YAG laser, and argon beam, and each type of laser has a specific use, and each type of surgery and tissue has a special color of laser light. the uses of the laser in the surgery are multiple. brain and liver tumors can be removed while not to damage the healthy tissue surrounding the tumors. Lymphatic vessels can be closed to reduce the spread of tumor cells and nerve endings to reduce pain after surgery, small bleeding blood vessels can be closed, in addition to removing warts and skin tumors. A general advantage of laser surgery is the absence of bleeding, reduced surgical infections, pain and wound size, and a short hospital stay. The recovery period after laser treatment depends on the type of pathological condition and the patient's health condition before treatment. In the end: the laser has successful surgical uses to obtain good results with minimal complications, and contrary to what is common, its medical uses are specific to certain diseases and not for all types of surgery. In addition, laser treatment requires modern and complex devices and experienced experts in their use

Impact of Synthetic Extracellular Matrices in Combination Therapy with Amniotic Allografting in the Treatment of Diabetic Foot Wounds: A Case Series.

Synthetic extracellular matrices are artificial polymers that are elongated and deposited as a matrix of nanofibers which mimic the native extracellular matrix. RenovoDerm® Anthem™ Wound Matrix (Columbus, Ohio) is comprised of polyglycolic acid and poly (L-lactide-co-caprolactone) which degrade by hydrolysis into a-hydroxy and fatty acids, lowering the pH and promoting regenerative cellular activity including angiogenesis. Amniotic allografts contain growth factors, cytokines, amino acids, extracellular matrix proteins, and hyaluronic acid which are recognized as intrinsic to the wound healing process. Synthetic extracellular matrices alone or in combination with amnio allografts do not have large bodies of evidence which demonstrate their effectiveness in the treatment of wounds. Presently, no prior studies have been performed to assess what impact these therapies may have on wound healing when used concurrently. The aim of this investigation was to assess whether a synergistic effect is produced with combination therapy using synthetic extracellular matrix and amniotic allografting. In this article, we present four cases of diabetic foot ulcerations treated with combination therapy. An amniotic fluid allograft, and/or membrane amniotic allograft, was implanted with a synthetic extracellular matrix dressing over top of the graft(s) at weekly intervals. All wounds demonstrated a greater than 80% decrease in wound size within four applications and achieved more than 95% wound closure after six applications.

Wound healing activity of hydromethanolic Dioscorea bulbifera extract on male wistar rat excision wound models

IntroductionWound healing is a complex physiological process that involves a series of coordinated events that recover structural and functional tissue integrity. The desire to promote nature-based solutions has led to the screening of various plants and herbs for their wound healing potentials with results showing the enhancement of various aspects of the wound healing process. Dioscorea bulbifera possesses a wide range of applications in folk remedies. In traditional Chinese medicine, it is used in the treatment of mumps, goitre, sore throat, gastric cancer and carcinoma of the rectum alone or in combination with other plants. The present study evaluated the effect of hydromethanolic extract of Dioscorea bulbifera (HEDB) on wound size, percentage of wound contraction and tissue hydroxyproline in male Wistar rat excision wound models. MethodsTwenty-five (25) male Wistar rats (180–200 g) were used for the study. They were divided into five (5) groups comprising five (5) animals per group. A circular excision wound (1.5–2 cm) was experimentally induced in each animal following ketamine anaesthesia. Groups I and II served as negative and positive controls and were treated with topical administration of distilled water and petroleum jelly respectively. Groups III, IV and V served as the experimental groups and were treated with 200, 400 and 800 mg/kg of HEDB topically. The diameter of the wound was measured and the wound area and percentage of wound contraction were calculated. Tissue hydroxyproline was determined using tissue excised from the healed portion of the dorsal surface of the animals via coulometric standard procedures. ResultsData from the study indicates that animals in the experimental groups (III, IV and V) had an earlier wound closure compared to the control. Animals treated with 200 mg/kg HEDB (Group III) showed complete wound closure by day 17, whereas animals treated with 400 mg/kg (Group IV) and 800 mg/kg HEDB (Group V) groups achieved complete wound closure by the 18th and 20th day of the experiment respectively. Similarly, all the animals in the experimental groups had significantly increased percentage of wound contraction compared to negative control (P < 0.05). Tissue hydroxyproline was significantly raised amongst the animals treated with 200 and 800 mg/kgHEDB compared to the negative control (p < 0.05). DiscussionEvidence from the study has shown that HEDB possesses potent wound healing activity as the present study has demonstrated that it reduced wound area, and increased wound contraction and tissue hydroxyproline on experimentally induced excision wound male Wistar rat models. These findings provide scientific evidence that validates the traditional medicinal application of Dioscorea bulbifera in promoting wound healing.

Can a prolonged healing pressure injury be benefited by using an AI mattress? A case study

BackgroundPressure injuries are a common and serious issue for bedridden residents in long-term-care facilities. Areas of bony prominences, such as the scapula, sacrum, and heels, are more likely to develop pressure injuries. The management of pressure injury wounds include dressing changes, repositioning, away from moisture, decreasing the occurrence of friction and shear, and more. Some supportive surfaces are also used for pressure injury cases such as gel pads, alternating pressure air mattresses, and air-fluidized beds. The aim of this case study was to determine whether the use of an artificial intelligent mattress can improve a nursing home resident with prolonged pressure injury.Case presentationA retrospective study design was conducted for this case study. A 79-year-old male developed a pressure injury in the sacrum. His pressure injury was initially at stage 4, with a score of 12 by the Braden scale. The PUSH score was 16. During 5.5 months of routine care plus the use of the traditional alternative air mattress, in the nursing home, the wound stayed in stage 3 but the PUSH score increased up to 11. An artificial intelligence mattress utilizing 3D InterSoft was used to detect the bony prominences and redistribute the external pressure of the skin. It implements a color guided schematic of 26 colors to indicate the amount of pressure of the skin.ResultsThe wound size was decreased and all eczema on the resident’s back diminished. The PUSH score was down to 6, as the artificial intelligent mattress was added into the routine care. The staff also reported that the resident’s quality of sleep improved and moaning decreased. The hemiplegic side is at greater risk of developing pressure injury.ConclusionsThis novice device appeared to accelerate wound healing in this case. In the future, more cases should be tested, and different care models or mattress can be explored.

Topical application of hyaluronic acid and amino acids in hard-to-heal wounds: a cost-effectiveness analysis.

The aim of this cost-effectiveness analysis was to estimate the monetary cost required to achieve a gain in health benefit. An analytic model to evaluate the cost-effectiveness of a topical medical device comprising a mixture of hyaluronic acid and amino acids (HA+AA medical device) (Vulnamin, Professional Dietetics SpA, Italy) as compared to standard of care (SoC) for hard-to-heal (chronic) wounds is presented. Retrospective data was analysed from a cohort of patients as well as information from published literature. For each paper, the following information was extracted: number of patients enrolled in each treatment arm and the results of prespecified reviewed outcomes. A total of six studies involving 378 patients were included in this pooled analysis. Findings showed that treatment with the HA+AA medical device has the potential to lower consumption of resources. With regards to wound healing, in both superficial and deep wounds, treatment benefits of the HA+AA medical device included: rapid wound size reduction; faster healing; reduction of dressing changes; reduced infection risk; and reduced treatment costs. Results showed the HA+AA medical device to be 32% more cost-effective than comparators in the treatment of hard-to-heal wounds (time horizon selected=six months). The findings of this analysis showed that treatment with the HA+AA medical device is a valid alternative to SoC care because it is cheaper, and its utility and effectiveness are greater. In addition, the results of the analysis showed a direct relationship between the time to complete healing and the increase in costs (increasing the period of time to reach complete healing increases the costs associated with the treatment).

Potassium permanganate in treatment of diabetic foot ulcer: A randomized clinical trial.

Diabetic foot ulcers (DFU) are a severe complication in diabetes patients, often resulting in significant morbidity and mortality due to non-healing. This study investigated the effectiveness of 5% topical potassium permanganate on these ulcers. A clinical trial was conducted on 23 patients with Wagner grade I and II DFU. Patients in the control group received standard treatment, while those in the intervention group also received 5% potassium permanganate topically. Data were recorded at 0, 7, 14, and 21 days for analysis. Among 23 patients studied, 7 (30.4%) were male and 16 (43.7%) female, with an average age of 59 ± 4 years. Both groups showed a statistically significant decrease in wound size and infection over time (p < 0.001). The intervention group, however, had a more substantial reduction in wound size and infection rate (p < 0.05). Potassium permanganate, when applied topically, is both well-tolerated and effective in enhancing wound healing and reducing infection in DFU, suggesting its potential as a complementary treatment.

Enhancing cutaneous wound healing: A study on the beneficial effects of nano-gelatin scaffold in rat models.

The challenges in achieving optimal outcomes for wound healing have persisted for decades, prompting ongoing exploration of interventions and management strategies. This study focuses on assessing the potential benefits of implementing a nano-gelatin scaffold for wound healing. Using a rat skin defect model, full-thickness incisional wounds were created on each side of the thoracic-lumbar regions after anesthesia. The wounds were left un-sutured, with one side covered by a gelatin nano-fibrous membrane and the other left uncovered. Wound size changes were measured on days 1, 4, 7, and 14, and on day 14, rats were sacrificed for tissue sample excision, examined with hematoxylin and eosin, and Masson's trichrome stain. Statistical comparisons were performed. The gelatin nanofibers exhibited a smooth surface with a fiber diameter of 260 ± 40 nm and porous structures with proper interconnectivity. Throughout the 14-day experimental period, significant differences in the percentage of wound closure were observed between the groups. Histological scores were higher in the experiment group, indicating less inflammation but dense and well-aligned collagen fiber formation. A preliminary clinical trial on diabetic ulcers also demonstrated promising results. This study highlights the potential of the nano-collagen fibrous membrane to reduce inflammatory infiltration and enhance fibroblast differentiation into myofibroblasts during the early stages of cutaneous wound healing. The nano-fibrous collagen membrane emerges as a promising candidate for promoting wound healing, with considerable potential for future therapeutic applications.