The minipuberty of infancy is a period of increased reproductive axis activity. Changes in reproductive hormone concentrations and organ size occur during this period, but longitudinal changes have not been well described. The objective of this study was to characterize ovarian growth trajectories and ovarian follicle development during the first 9months of life in a large longitudinal cohort of healthy girls. Data from the Infant Feeding and Early Development Study, a longitudinal cohort study of oestrogen-responsive outcomes in healthy infants, were used to estimate ovarian growth trajectories and describe the presence of ovarian antral follicles in girls 0-9months old. Ovarian ultrasound evaluations were performed on the infants within 72hours of birth (newborn visit) and at 4, 8, 16, 24, and 32weeks of age. Mixed-effects regression splines were used to characterize changes in ovarian volume during infancy and assess the association between the presence of ovarian follicles at the newborn visit and ovarian growth. This analysis included 163 girls with two or more ovarian ultrasounds in the study. Results from the estimated overall ovarian growth trajectory show that ovarian volume increases more than sixfold during the first 16weeks after birth and then remains relatively stable in the later weeks of infancy. Among girls with observable ovaries at the newborn visit (n=133), girls with at least one visible ovarian follicle showed more rapid initial ovarian growth compared with girls without visible follicles. Infant ovarian volume increased to a peak at 16weeks, which was influenced by the number and size of developing follicles. This research contributes to future development of reference ranges for postnatal ovarian growth in healthy, term infants.